pax_global_header00006660000000000000000000000064140672271310014515gustar00rootroot0000000000000052 comment=f0cb3b34907b9fdf204fda3a36f61cf07b50f68f Filtlong-0.2.1/000077500000000000000000000000001406722713100132735ustar00rootroot00000000000000Filtlong-0.2.1/.gitignore000066400000000000000000000002351406722713100152630ustar00rootroot00000000000000# Mac stuff .DS_Store ._.DS_Store # Built files src/*.o bin # CLion .idea/ cmake-build-debug CMakeLists.txt # Pycharm test/__pycache__/ notes gh-md-toc Filtlong-0.2.1/LICENSE000066400000000000000000001045131406722713100143040ustar00rootroot00000000000000 GNU GENERAL PUBLIC LICENSE Version 3, 29 June 2007 Copyright (C) 2007 Free Software Foundation, Inc. Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed. Preamble The GNU General Public License is a free, copyleft license for software and other kinds of works. 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Filtlong-0.2.1/Makefile000066400000000000000000000023071406722713100147350ustar00rootroot00000000000000# Example commands: # make (build in release mode) # make debug (build in debug mode) # make clean (deletes *.o files, which aren't required to run the aligner) # make distclean (deletes *.o files and the binary) # make CXX=g++-5 (build with a particular compiler) # make CXXFLAGS="-Werror -g3" (build with particular compiler flags) # CXX and CXXFLAGS can be overridden by the user. CXX ?= g++ CXXFLAGS ?= -Wall -Wextra -pedantic -mtune=native # These flags are required for the build to work. LIB = -lz FLAGS = -std=c++11 # Different debug/optimisation levels for debug/release builds. DEBUGFLAGS = -g RELEASEFLAGS = -O3 TARGET = bin/filtlong SHELL = /bin/sh SOURCES = $(shell find src -name "*.cpp") HEADERS = $(shell find src -name "*.h") OBJECTS = $(SOURCES:.cpp=.o) .PHONY: release release: FLAGS+=$(RELEASEFLAGS) release: $(TARGET) .PHONY: debug debug: FLAGS+=$(DEBUGFLAGS) debug: $(TARGET) dir_guard=@mkdir -p $(@D) $(TARGET): $(OBJECTS) $(dir_guard) $(CXX) $(FLAGS) $(CXXFLAGS) -o $(TARGET) $(OBJECTS) $(LIB) clean: $(RM) $(OBJECTS) distclean: clean $(RM) $(TARGET) %.o: %.cpp $(HEADERS) $(CXX) $(FLAGS) $(CXXFLAGS) -c -o $@ $< Filtlong-0.2.1/README.md000066400000000000000000000557651406722713100145740ustar00rootroot00000000000000

Filtlong

Filtlong is a tool for filtering long reads by quality. It can take a set of long reads and produce a smaller, better subset. It uses both read length (longer is better) and read identity (higher is better) when choosing which reads pass the filter. ## Table of contents * [Requirements](#requirements) * [Installation](#installation) * [Example commands (quick)](#example-commands-quick) * [Example commands (detailed)](#example-commands-detailed) * [Full usage](#full-usage) * [Method](#method) * [Read scoring](#read-scoring) * [Trimming and splitting](#trimming-and-splitting) * [FAQ](#faq) * [Acknowledgements](#acknowledgements) * [License](#license) ## Requirements * Linux or macOS * C++ compiler (GCC 4.8 or later should work) * zlib (usually included with Linux/macOS) ## Installation Filtlong builds into a stand-alone executable: ``` git clone https://github.com/rrwick/Filtlong.git cd Filtlong make -j bin/filtlong -h ``` If you plan on using Filtlong a lot, I'd recommend copying it to a directory in your PATH: ``` cp bin/filtlong /usr/local/bin ``` ## Example commands (quick) ### Without an external reference ``` filtlong --min_length 1000 --keep_percent 90 --target_bases 500000000 input.fastq.gz | gzip > output.fastq.gz ``` ### With an external reference ``` filtlong -1 illumina_1.fastq.gz -2 illumina_2.fastq.gz --min_length 1000 --keep_percent 90 --target_bases 500000000 --trim --split 500 input.fastq.gz | gzip > output.fastq.gz ``` ## Example commands (detailed) These examples use a 1.3 Gbp read set that was part of a [barcoded 1D MinION run](https://github.com/rrwick/Bacterial-genome-assemblies-with-multiplex-MinION-sequencing). I assessed read identity by aligning the reads to a completed assembly using [minimap2](https://github.com/lh3/minimap2) and [this script](misc/read_length_identity.py).
unfiltered Here is what the reads look like before Filtlong. Each dot is a read and the marginal histograms show the length distribution (top) and identity distribution (right).

The length N50 is 24,077 bp (i.e. half the bases are in a read 24,077 bp long or longer).
The identity N50 is 85.60% (i.e. half the bases are in a read with 85.60% or higher identity).
### Without an external reference When you run Filtlong without an external reference, it judges read quality using the Phred quality scores in the FASTQ file. ``` filtlong --min_length 1000 --keep_percent 90 --target_bases 500000000 input.fastq.gz | gzip > output.fastq.gz ``` * `--min_length 1000` ← Discard any read which is shorter than 1 kbp. * `--keep_percent 90` ← Throw out the worst 10% of reads. This is measured by bp, not by read count. So this option throws out the worst 10% of read bases. * `--target_bases 500000000` ← Remove the worst reads until only 500 Mbp remain, useful for very large read sets. If the input read set is less than 500 Mbp, this setting will have no effect. * `input.fastq.gz` ← The input long reads to be filtered (must be FASTQ format). * `| gzip > output.fastq.gz` ← Filtlong outputs the filtered reads to stdout. Pipe to gzip to keep the file size down.
without_reference Filtlong has cut the original 1.3 Gbp of reads down to a much better 500 Mbp subset. Short reads and low identity reads have been mostly removed.

Length N50 = 36,827 bp
Identity N50 = 88.53%
### With Illumina read reference When an external reference is provided, Filtlong ignores the Phred quality scores and instead judges read quality using k-mer matches to the reference (a more accurate gauge of quality). FASTA input reads are allowed when a reference is provided, and if used, Filtlong will produce FASTA output. ``` filtlong -1 illumina_1.fastq.gz -2 illumina_2.fastq.gz --min_length 1000 --keep_percent 90 --target_bases 500000000 input.fastq.gz | gzip > output.fastq.gz ``` * `-1 illumina_1.fastq.gz -2 illumina_2.fastq.gz` ← Use Illumina reads as an external reference. You can instead use `-a` to provide an assembly as a reference, but Illumina reads are preferable if available.
with_reference With an external reference, Filtlong is better able to judge read quality, and now most remaining reads are 85% identity or better. The length distribution has suffered a bit, however, because when outputting a fixed amount of reads (500 Mbp in this case), there is a trade-off between length and quality.

Length N50 = 28,713 bp
Identity N50 = 88.94%
### With trimming and splitting When an external reference is provided, you can turn on read trimming and splitting to further increase read quality. See [Trimming and splitting](#trimming-and-splitting) for more information. ``` filtlong -1 illumina_1.fastq.gz -2 illumina_2.fastq.gz --min_length 1000 --keep_percent 90 --target_bases 500000000 --trim --split 500 input.fastq.gz | gzip > output.fastq.gz ``` * `--trim` ← Trim bases from the start and end of reads which do not match a k-mer in the reference. This ensures the each read starts and ends with good sequence. * `--split 500` ← Split reads whenever 500 consecutive bases fail to match a k-mer in the reference. This serves to remove very poor parts of reads while keeping the good parts. A lower value will split more aggressively and a higher value will be more conservative.
trim_split Trimming and splitting has further improved the read identity. This is especially apparent at the short side of the length distribution where a lot more reads now exceed 92% identity. Some of these high-identity shorter reads will be parts of longer reads which were split.

Length N50 = 28,407 bp
Identity N50 = 89.37%
### Length priority You can adjust the relative importance of Filtlong's read metrics. In this example, more weight is given to read length. ``` filtlong -1 illumina_1.fastq.gz -2 illumina_2.fastq.gz --min_length 1000 --keep_percent 90 --target_bases 500000000 --trim --split 1000 --length_weight 10 input.fastq.gz | gzip > output.fastq.gz ``` * `--length_weight 10` ← A length weight of 10 (instead of the default of 1) makes read length more important when choosing the best reads. * `--split 1000` ← This larger split value makes Filtlong less likely to split a read. I.e. a read has to have a _lot_ of consecutive bad bases before it gets split. This helps to keep the output reads longer.
length_priority These settings greatly improve the length distribution, but the length-quality trade-off results in more low-identity reads.

Length N50 = 43,877 bp
Identity N50 = 87.89%
### Quality priority You can adjust the relative importance of Filtlong's read metrics. In this example, more weight is given to read quality. ``` filtlong -1 illumina_1.fastq.gz -2 illumina_2.fastq.gz --min_length 1000 --keep_percent 90 --target_bases 500000000 --trim --split 100 --mean_q_weight 10 input.fastq.gz | gzip > output.fastq.gz ``` * `--mean_q_weight 10` ← A mean quality weight of 10 (instead of the default of 1) makes mean read quality more important when choosing the best reads. * `--split 100` ← This smaller split value makes Filtlong split reads more often. I.e. even a relatively small stretch of bad bases will result in a split, giving shorter reads but of higher quality.
length_priority These settings produce the best identity distribution, with most reads now 87% identity or better. Length now has a relatively lower weight in the score function, so many shorter reads are kept.

Length N50 = 14,127 bp
Identity N50 = 89.83%
## Full usage ``` usage: filtlong {OPTIONS} [input_reads] Filtlong: a quality filtering tool for Nanopore and PacBio reads positional arguments: input_reads input long reads to be filtered optional arguments: output thresholds: -t[int], --target_bases [int] keep only the best reads up to this many total bases -p[float], --keep_percent [float] keep only this percentage of the best reads (measured by bases) --min_length [int] minimum length threshold --max_length [int] maximum length threshold --min_mean_q [float] minimum mean quality threshold --min_window_q [float] minimum window quality threshold external references (if provided, read quality will be determined using these instead of from the Phred scores): -a[file], --assembly [file] reference assembly in FASTA format -1[file], --illumina_1 [file] reference Illumina reads in FASTQ format -2[file], --illumina_2 [file] reference Illumina reads in FASTQ format score weights (control the relative contribution of each score to the final read score): --length_weight [float] weight given to the length score (default: 1) --mean_q_weight [float] weight given to the mean quality score (default: 1) --window_q_weight [float] weight given to the window quality score (default: 1) read manipulation: --trim trim non-k-mer-matching bases from start/end of reads --split [split] split reads at this many (or more) consecutive non-k-mer-matching bases other: --window_size [int] size of sliding window used when measuring window quality (default: 250) --verbose verbose output to stderr with info for each read --version display the program version and quit -h, --help display this help menu For more information, go to: https://github.com/rrwick/Filtlong ``` ## Method When run, Filtlong carries out the following steps: 1. If an external reference was provided, hash all of the reference's 16-mers. * If the reference is an assembly, then Filtlong simply hashes all 16-mers in the assembly. * If the reference is in Illumina reads, then the 16-mer has to be encountered a few times before it's hashed (to avoid hashing 16-mers that result from read errors). 2. Look at each of the input reads to get length and quality information. * If a read fails to meet any of the hard thresholds (`--min_length`, `--max_length`, `--min_mean_q` or `--min_window_q`) then it is marked as 'fail' now. * Note that `--min_mean_q` and `--min_window_q` are expressed as sequence percent identities from 0-100 (see how this is calculated in the [Read scoring](#read-scoring) section), not as PHRED scores. * For advice on setting minimum quality thresholds, see [Filtlong's scripts directory](https://github.com/rrwick/Filtlong/tree/main/scripts). * If `--trim` or `--split` was used, then 'child' reads are made here (see [Trimming and splitting](#trimming-and-splitting)). * If `--verbose` was used, display detailed information about the read length, quality and trimming/splitting. 3. Gather up all reads eligible for output. If neither `--trim` nor `--split` was used, this is simply the original set of reads. If `--trim` or `--split` was used, then the child reads replace the original reads. 4. Give each read a final score (see [Read scoring](#read-scoring) for more information). 4. If `--target_bases` and/or `--keep_percent` was used, sort the reads by their final score and set an appropriate threshold. Reads which fall below the threshold are marked as 'fail'. * If both `--target_bases` and `--keep_percent` are used, the threshold is set to the more stringent of the two. 5. Output all reads which didn't fail to stdout. * Reads are outputted in the same order as the input file (not in in quality-sorted order). ## Read scoring Reads are scored based on three separate metrics: length, mean quality and window quality: * __Length score__
The length score is pretty simple: longer is better. A read length of 5 kbp is considered mediocre and gives a score of 50. Shorter reads get a lower score and the score approaches 100 as the read length goes to infinity ([here is a graph of the score function](https://www.desmos.com/calculator/5m1lwd7fye)). * __Mean quality__
A read's mean quality is calculated in two different ways, depending on whether an external reference was used: * If there is no reference, the mean quality is the mean read identity as indicated by the Phred quality scores. For example, consider a read where all the fastq quality characters are `+`. The qscores for each base are 10 which equates to a 90% chance of being correct. This read would then have a mean quality score of 90. * If an external reference was used, then Filtlong tallies up the 16-mers in the reference. Read bases are then given a quality of either 100 (contained in a 16-mer from the reference) or 0 (not contained in a 16-mer from the reference). The read's mean quality is a mean of its base qualities. * __Mean quality score__
Read mean qualities are converted to a z-score and scaled to the range 0-100 to make the mean quality score. This means that the read with the worst mean quality in the input set will get a mean quality score of 0 and the read with the best mean quality will get a mean quality score of 100. * __Window quality__
The window quality is determined by looking at the mean quality for a sliding window over the read. The default window size is 250 but can be changed with `--window_size`. The final window quality is the lowest value for the read, i.e. the quality of the read's weakest point. * __Window quality score__
The window quality score is the mean quality score scaled down by the window quality to mean quality ratio. For example, consider a read with a mean quality of 90 which resulted in a mean quality score of 60. If the window quality was 45 (half the mean quality), then the window quality score will be 30 (half the mean quality score). __Final score__
The read's final score is a combination of the three component scores: * Filtlong first takes the weighted geometric mean of the length score and the mean quality score. The weights are equal (both 1) by default, but you can adjust this with `--length_weight` and `--mean_q_weight` to make one or the other more important. * The score is then scaled down by a factor based on the window quality score to mean quality score ratio. This factor is adjusted by the relative strength of the window quality weight (`--window_q_weight`). * Expressed mathematically:

score equation

The final score which is used to determine thresholds for the `--keep_percent` and `--target_bases` options and therefore determines whether a read is included in the output. ## Trimming and splitting If `--trim` or `--split` are used, then each read can result in one or more 'child' reads. These options can only be used with an external reference (i.e. Filtlong will not trim or split based on Phred quality scores). ### Trim example Consider the following example read. Bases which match a 16-mer to the reference are bold:

Trim example 1

If Filtlong is run with `--trim`, any non-matching bases at the start and end are removed to produce this child read:

Trim example 2

Only the child read (not the original read) is now eligible for outputting. It's a bit shorter than the original read, but its mean quality will be better. ### Split example If Filtlong is run with `--trim --split 20`, then in addition to trimming the ends, any run of non-matching bases 20 or longer will be removed. Using the same example, this results in two separate child reads:

Split example 1

These child reads are much shorter than the original read but are much higher quality. Note that a split setting of 20 was used for this toy example but is very low for a real run of Filtlong – it would be quite aggressive and result in reads being split into very many pieces. A value like 500 is more practical. For an extreme example, this is what you'd get if you used `--trim --split 1`:

Split example 2

Now _any_ run of non-matching bases is removed, regardless of length. The read is split into 3 child reads, each with perfect quality. Again, such a low setting would probably not be practical for a real read set. ### Real read example Here's a real example of Filtlong's trimming and splitting. The output shown is what you'd see if you ran Filtlong with the `--verbose` option. The original read is quite long and has a decent mean quality. However, its window quality is zero, indicating that the read has windows where no 16-mers match the reference: ``` bf09f0e9-d27d-4a18-bced-f2536b62b3e5_Basecall_Alignment_template length = 117786 mean quality = 53.02 window quality = 0.00 ``` The read's bad ranges are the coordinates non-matching start/end regions (because of `--trim`) or runs of non-matching bases (because of `--split`). The child ranges are the inverse, bases that aren't in the bad ranges: ``` bad ranges = 0-25, 71401-71745, 72393-72683, 72742-73049, 77279-77627, 78710-79055, 85575-85947, 86620-86877, 89397-89682, 91451-91782, 94415-94764, 96010-96306, 96604-96886, 98691-99176, 102349-102655, 102913-103286, 103488-103828, 106124-106397, 113277-113581, 117784-117786 child ranges = 25-71401, 71745-72393, 72683-72742, 73049-77279, 77627-78710, 79055-85575, 85947-86620, 86877-89397, 89682-91451, 91782-94415, 94764-96010, 96306-96604, 96886-98691, 99176-102349, 102655-102913, 103286-103488, 103828-106124, 106397-113277, 113581-117784 ``` Each child range results in a child read. Here are the first few of them: ``` bf09f0e9-d27d-4a18-bced-f2536b62b3e5_Basecall_Alignment_template_26-71401 length = 71376 mean quality = 74.09 window quality = 6.80 bf09f0e9-d27d-4a18-bced-f2536b62b3e5_Basecall_Alignment_template_71746-72393 length = 648 mean quality = 15.12 window quality = 6.40 bf09f0e9-d27d-4a18-bced-f2536b62b3e5_Basecall_Alignment_template_72684-72742 length = 59 mean quality = 98.31 window quality = 98.31 bf09f0e9-d27d-4a18-bced-f2536b62b3e5_Basecall_Alignment_template_73050-77279 length = 4230 mean quality = 25.11 window quality = 6.40 ``` And here is the read visualised with child ranges in blue and bad ranges in red (values are length in kbp):

Real read trim split

Trimming/splitting has turned a very long read with some bad regions into some smaller reads (one of which is still quite long) without bad regions. Depending on the output thresholds (like `--min_length` and `--keep_percent`) many of the smaller child reads may fail and won't be outputted, leaving us with just the longest child reads. ## FAQ * __Why is the logo a hot dog?__ * It's a _footlong_ hot dog. Filtlong... footlong... get it?! Not all my Australian colleagues were familiar with footlong hot dogs, so maybe they are a US thing. Leave it to Americans to take a fatty, unhealthy food and make an extra large version :smile: * __Why does Filtlong use a k-mer size of 16 when hashing reference k-mers?__ * Because I can fit a 16-mer sequence neatly into a 32-bit unsigned integer ([like this](https://github.com/rrwick/Filtlong/blob/ce99bc062bb1611f38deb5e7502cfb66b98598ae/src/kmers.cpp#L222-L229)). It also seemed like a good balance between small k-mers where there's more risk of chance matches and large k-mers where noisy long reads will struggle to match. I haven't empirically tested the effectiveness of different k-mer sizes though – might be good to check out for a future version of Filtlong. * __Is it ever a _bad_ idea to use an external reference (like Illumina reads)?__ * If you provide Filtlong with an external reference, then long read qualities will be determined solely based on their k-mer matches to the reference. This is great if your Illumina reads have complete coverage. However, if they have poor coverage (i.e. parts of the genome are not represented in the Illumina reads), then long reads which span the poor-Illumina-coverage part of the genome may be erroneously considered low-quality. * Similarly, if there are genuine biological differences between your read sets, then the long reads may be erroneously considered low-quality in regions of difference. E.g. if your long read sample has a plasmid which isn't in your Illumina reads, then Filtlong could remove long reads from that plasmid. * If you think either of these cases applies to you, I'd recommend _against_ using an external reference. * __Are FASTA inputs allowed?__ * Yes, but only if you use an external reference (with the `-a` or `-1`/`-2` options). This is because Filtlong needs to assess read quality, and a FASTA file contains no quality information. If you use a FASTA input, Filtlong will produce a FASTA output. ## Acknowledgements I owe many thanks to [Kat Holt](https://holtlab.net/) and [Louise Judd](https://scholar.google.com.au/citations?user=eO22mYUAAAAJ) for keeping me well supplied with Nanopore reads. 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I used it to make the scatterplot/histogram figures in the Filtlong README. It uses a particularly strict definition of read identity: all bases are considered and unaligned bases are assigned an identity of 0. So if a read had half of its bases align with an identity of 90% and the other half is unaligned, then the read's final identity would be 45%. Example commands: filtlong {OPTIONS} input.fastq.gz | gzip > filtered.fastq.gz minimap2 -x map10k -t 16 -c reference.fasta filtered.fastq.gz > alignments.paf read_length_identity.py alignments.paf > read_stats.tsv This file is part of Filtlong. Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with Filtlong. If not, see . """ import sys import collections import statistics def main(): read_lengths = {} read_alignments = collections.defaultdict(list) paf_filename = sys.argv[1] with open(paf_filename, 'rt') as paf: for line in paf: paf_parts = line.strip().split('\t') if len(paf_parts) < 11: continue read_name = paf_parts[0] read_length = int(paf_parts[1]) read_lengths[read_name] = read_length start = int(paf_parts[2]) end = int(paf_parts[3]) identity = 100.0 * int(paf_parts[9]) / int(paf_parts[10]) read_alignments[read_name].append((start, end, identity)) print('\t'.join(['Name', 'Length', 'Identity'])) for read_name, read_length in read_lengths.items(): alignments = read_alignments[read_name] identity_by_base = [0.0] * read_length for start, end, identity in alignments: for i in range(start, end): if identity > identity_by_base[i]: identity_by_base[i] = identity whole_read_identity = statistics.mean(identity_by_base) print('\t'.join([read_name, str(read_length), str(whole_read_identity)])) if __name__ == '__main__': main() Filtlong-0.2.1/misc/score_equation.png000066400000000000000000001414771406722713100177720ustar00rootroot00000000000000PNG  IHDRS pHYsXXm7IDATxqu&AA*0R LW@I+R T W};;1iFx$HȽFpuE箍,wBWk{72kߦBg;ʜG]}ǖ9P_r,kSԝmqoԑ]DS_q|/v/]1(||LW]V9)W4^?Tev>qNu[2<PӘsZFBW&(!bYoS(Xk˨p*)H}߫XFhZS&9"¨Fz_(oLbYhogLMH&u)UȄUF%"5D޾Za:=H4*pzs_.]O)E2ۑ်~cQ[_s|nDObدSa`mħaڨYzw)Q'bȩ(zo6*7Ź6){u=T:n'ֽ8q'C= mO{[,Zm5bHu45MOL&kѤa}ux5a3p43eVh&^u SO8ey{>mmtH">ǯpmKXv1<{DP չ=st VԷ.ua;"yke4AâÄ,'սQ }lukQ7>1^8`>տƏ/gJDƙRfg_f=SI7غv?c,s~BXN=^)}>"riz2e&SRvdJQbgL sQ[xM\f|b:yLMX {*L94\y2e6Gre12Xo{*Aq29wL-LHas)>MWSb~&"iRka̮|IJ֦vxmp2?rId D(4딅aEMlItޮx?G|mNx~e&|M}.,Fܥ>cC'j.~]Q>DB⹺h{\{)U̝p&wsAa8.sWxu%9}{W9k ϫ̰Md9{_G,tb'XM\\9Uyضxt&Mל21PHǑ 5e"UIUi:XT_~+WOLr&7'ȹmN$S6Fǚr7bM"LUyyXR0gHO8nϹ} =87tVy$S$ʧ̈́_G] žX;՛t>ivpqhXnrxȿ?k2?/99:<Ѥr,=Oէ6iئxoG̣L=Tr]63+I5}ߟ<rtEb7AqS4~6G6fާH2=0ru@LYUi2igUɔx&lU5t Q~XVP:9pE|ĺ?,u#{Z&6e+^_W۬kc}ɾtQ_6⵮e,秏hNLy1OKfr1P_#.]+f3XW⽩׽>@lTeFVE?cGIJ1"nO*jMUٲ}<&{{ cZhb9i]%hu1IQ^}_k>OxcI&'*=M4ξUr\㕿X.^tU.b6"ہ}mc)2=e^E=}bYw 9c1I6Mv1}:u};>K!ʼ6\ruok_S3<9 KSU)^Ws')0}6`y0_$SL>HNg &~J1< by60_]d0_) )͞dpnV|)>E"aR'S>\d:\H.$ ż(r@%#JpM.eu6rr4/9O)?2 orls|Jx~#-O?x~~JpF|pu|z&qn?˱:GӘ{l?k+=SC}aLإS3s9Yȿs 47{/,$Ňoх1aNk,Oic5g.cPIzOqB&(w`]<G߽ɔEKȱZ V9=sr@ٛ]o&|1 |$S Lúz5{{ >- {ct|$SRPOJoz٠ƚ&we;9_4)Lx41ǗmY#irq Ry711G."e>i&GW,Sxe|֝m1D?9nr<>(8]ky{~6qb+0L+XȮv+Rt+V9s!|s0qel۾3(QL_!/A>`(9_-5alxT_ =#Ɖ =3MeVzcA .n]8"=lK}~F=j=);R⺽Щ=Ty I5tj8.EFL}:n Աzn}{ث3 ɼ` be^[ J=4B!ӟH]bT5BJ7R2Ŀ+)i7bgSok? 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## Example with Illumina reads as an external reference This example uses the same dataset as the previous one, but the qualities now come from k-mer matches to Illumina reads. The qualities are therefore different but the read length information is unchanged. __Command__: ``` path/to/Filtlong/scripts/read_info_histograms.sh long_reads.fastq.gz illumina_1.fastq.gz illumina_2.fastq.gz ``` __Output__: ``` READ SET SUMMARY ---------------- number of reads: 62,313 number of bases: 498,400,580 N50 read length: 16,730 READ LENGTHS ------------ Each ∎ represents 1,044,446 bases 0 - 3799 [45,923,686]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 3799 - 7597 [73,111,261]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 7597 - 11396 [63,135,675]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 11396 - 15195 [49,558,377]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 15195 - 18994 [40,910,839]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 18994 - 22792 [34,441,187]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 22792 - 26591 [28,738,160]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 26591 - 30390 [24,738,026]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 30390 - 34189 [22,096,602]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 34189 - 37987 [18,985,609]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 37987 - 41786 [15,406,318]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 41786 - 45585 [12,755,023]: ∎∎∎∎∎∎∎∎∎∎∎∎ 45585 - 49383 [12,919,019]: ∎∎∎∎∎∎∎∎∎∎∎∎ 49383 - 53182 [ 9,529,903]: ∎∎∎∎∎∎∎∎∎ 53182 - 56981 [ 7,744,484]: ∎∎∎∎∎∎∎ 56981 - 60780 [ 6,403,620]: ∎∎∎∎∎∎ 60780 - 64578 [ 5,626,494]: ∎∎∎∎∎ 64578 - 68377 [ 5,434,617]: ∎∎∎∎∎ 68377 - 72176 [ 3,851,906]: ∎∎∎ 72176 - 75974 [ 3,104,518]: ∎∎ 75974 - 79773 [ 2,723,174]: ∎∎ 79773 - 83572 [ 2,445,516]: ∎∎ 83572 - 87371 [ 1,622,746]: ∎ 87371 - 91169 [ 1,424,798]: ∎ 91169 - 94968 [ 1,018,819]: 94968 - 98767 [ 1,254,227]: ∎ 98767 - 102566 [ 1,000,129]: 102566 - 106364 [ 206,593]: 106364 - 110163 [ 217,310]: 110163 - 113962 [ 561,474]: 113962 - 117760 [ 233,150]: 117760 - 121559 [ 476,864]: 121559 - 125358 [ 248,209]: 125358 - 129157 [ 0]: 129157 - 132955 [ 262,547]: 132955 - 136754 [ 0]: 136754 - 140553 [ 137,751]: 140553 - 144352 [ 0]: 144352 - 148150 [ 0]: 148150 - 151949 [ 151,949]: MEAN QUALITIES -------------- Each ∎ represents 677,887 bases 0.0 - 2.5 [ 67,263]: 2.5 - 4.9 [ 82,815]: 4.9 - 7.4 [ 256,950]: 7.4 - 9.9 [ 749,158]: ∎ 9.9 - 12.3 [ 1,152,658]: ∎ 12.3 - 14.8 [ 1,754,082]: ∎∎ 14.8 - 17.3 [ 3,059,964]: ∎∎∎∎ 17.3 - 19.7 [ 5,408,847]: ∎∎∎∎∎∎∎ 19.7 - 22.2 [ 7,510,681]: ∎∎∎∎∎∎∎∎∎∎∎ 22.2 - 24.6 [ 4,006,337]: ∎∎∎∎∎ 24.6 - 27.1 [ 2,801,778]: ∎∎∎∎ 27.1 - 29.6 [ 2,502,685]: ∎∎∎ 29.6 - 32.0 [ 3,258,647]: ∎∎∎∎ 32.0 - 34.5 [ 3,435,155]: ∎∎∎∎∎ 34.5 - 37.0 [ 4,278,777]: ∎∎∎∎∎∎ 37.0 - 39.4 [ 4,983,333]: ∎∎∎∎∎∎∎ 39.4 - 41.9 [ 6,439,521]: ∎∎∎∎∎∎∎∎∎ 41.9 - 44.4 [ 7,588,641]: ∎∎∎∎∎∎∎∎∎∎∎ 44.4 - 46.8 [ 8,908,943]: ∎∎∎∎∎∎∎∎∎∎∎∎∎ 46.8 - 49.3 [10,001,462]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 49.3 - 51.8 [12,713,431]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 51.8 - 54.2 [16,136,550]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 54.2 - 56.7 [18,115,381]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 56.7 - 59.1 [21,659,142]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 59.1 - 61.6 [23,166,427]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 61.6 - 64.1 [25,919,731]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 64.1 - 66.5 [31,712,427]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 66.5 - 69.0 [36,552,017]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 69.0 - 71.5 [41,159,323]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 71.5 - 73.9 [46,002,774]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 73.9 - 76.4 [47,452,136]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 76.4 - 78.9 [46,485,175]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 78.9 - 81.3 [32,871,399]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 81.3 - 83.8 [15,072,800]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 83.8 - 86.3 [ 3,755,240]: ∎∎∎∎∎ 86.3 - 88.7 [ 961,203]: ∎ 88.7 - 91.2 [ 370,319]: 91.2 - 93.7 [ 36,939]: 93.7 - 96.1 [ 8,536]: 96.1 - 98.6 [ 1,933]: WINDOW QUALITIES ---------------- Each ∎ represents 1,223,100 bases 0.0 - 2.5 [85,617,025]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 2.5 - 4.9 [ 4,646,208]: ∎∎∎ 4.9 - 7.4 [42,058,882]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 7.4 - 9.9 [20,326,766]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 9.9 - 12.3 [12,648,808]: ∎∎∎∎∎∎∎∎∎∎ 12.3 - 14.8 [33,893,701]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 14.8 - 17.3 [24,865,373]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 17.3 - 19.8 [20,757,969]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 19.8 - 22.2 [24,508,676]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 22.2 - 24.7 [24,211,150]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 24.7 - 27.2 [22,883,942]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 27.2 - 29.6 [25,860,478]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 29.6 - 32.1 [21,054,189]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 32.1 - 34.6 [20,389,975]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 34.6 - 37.0 [17,721,925]: ∎∎∎∎∎∎∎∎∎∎∎∎∎∎ 37.0 - 39.5 [16,808,916]: ∎∎∎∎∎∎∎∎∎∎∎∎∎ 39.5 - 42.0 [14,247,451]: ∎∎∎∎∎∎∎∎∎∎∎ 42.0 - 44.5 [16,429,129]: ∎∎∎∎∎∎∎∎∎∎∎∎∎ 44.5 - 46.9 [11,544,406]: ∎∎∎∎∎∎∎∎∎ 46.9 - 49.4 [ 9,910,130]: ∎∎∎∎∎∎∎∎ 49.4 - 51.9 [ 7,622,653]: ∎∎∎∎∎∎ 51.9 - 54.3 [ 5,814,027]: ∎∎∎∎ 54.3 - 56.8 [ 4,865,746]: ∎∎∎ 56.8 - 59.3 [ 3,159,894]: ∎∎ 59.3 - 61.8 [ 2,174,103]: ∎ 61.8 - 64.2 [ 1,399,105]: ∎ 64.2 - 66.7 [ 1,003,958]: 66.7 - 69.2 [ 637,184]: 69.2 - 71.6 [ 508,873]: 71.6 - 74.1 [ 255,636]: 74.1 - 76.6 [ 183,048]: 76.6 - 79.0 [ 145,158]: 79.0 - 81.5 [ 92,925]: 81.5 - 84.0 [ 61,294]: 84.0 - 86.5 [ 46,942]: 86.5 - 88.9 [ 20,743]: 88.9 - 91.4 [ 14,950]: 91.4 - 93.9 [ 5,187]: 93.9 - 96.3 [ 3,171]: 96.3 - 98.8 [ 884]: ``` There is an interesting bimodel distribution in the mean quality histogram. I hypothesise that the larger (higher quality) component is from real reads, while the smaller (lower quality) component is junk. To filter conservatively, I would perhaps just use `--min_mean_q 30`. To filter more aggressively, I would also use `--min_window_q 5`. Filtlong-0.2.1/scripts/histogram.py000077500000000000000000000273111406722713100173400ustar00rootroot00000000000000#!/usr/bin/env python # -*- coding: utf-8 -*- # # Copyright 2010 Bitly # # Licensed under the Apache License, Version 2.0 (the "License"); you may # not use this file except in compliance with the License. You may obtain # a copy of the License at # # http://www.apache.org/licenses/LICENSE-2.0 # # Unless required by applicable law or agreed to in writing, software # distributed under the License is distributed on an "AS IS" BASIS, WITHOUT # WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the # License for the specific language governing permissions and limitations # under the License. """ Generate a text format histogram This is a loose port to python of the Perl version at http://www.pandamatak.com/people/anand/xfer/histo https://github.com/bitly/data_hacks This version of the script comes with Filtlong (github.com/rrwick/Filtlong) and was slightly modified to better suit statistics of long sequencing reads. """ from __future__ import print_function from __future__ import division import sys from decimal import Decimal import logging import math from optparse import OptionParser from collections import namedtuple class MVSD(object): "A class that calculates a running Mean / Variance / Standard Deviation" def __init__(self): self.is_started = False self.ss = Decimal(0) # (running) sum of square deviations from mean self.m = Decimal(0) # (running) mean self.total_w = Decimal(0) # weight of items seen def add(self, x, w=1): "add another datapoint to the Mean / Variance / Standard Deviation" if not isinstance(x, Decimal): x = Decimal(x) if not self.is_started: self.m = x self.ss = Decimal(0) self.total_w = w self.is_started = True else: temp_w = self.total_w + w self.ss += (self.total_w * w * (x - self.m) * (x - self.m)) // temp_w self.m += (x - self.m) // temp_w self.total_w = temp_w def var(self): return self.ss // self.total_w def sd(self): return math.sqrt(self.var()) def mean(self): return self.m DataPoint = namedtuple('DataPoint', ['value', 'count']) def test_mvsd(): mvsd = MVSD() for x in range(10): mvsd.add(x) assert '%.2f' % mvsd.mean() == "4.50" assert '%.2f' % mvsd.var() == "8.25" assert '%.14f' % mvsd.sd() == "2.87228132326901" def load_stream(input_stream, agg_value_key, agg_key_value): for line in input_stream: clean_line = line.strip() if not clean_line: # skip empty lines (ie: newlines) continue if clean_line[0] in ['"', "'"]: clean_line = clean_line.strip("\"'") try: if agg_key_value: key, value = clean_line.rstrip().rsplit(None, 1) yield DataPoint(Decimal(key), int(value)) elif agg_value_key: value, key = clean_line.lstrip().split(None, 1) yield DataPoint(Decimal(key), int(value)) else: yield DataPoint(Decimal(clean_line), 1) except: logging.exception('failed %r', line) print("invalid line %r" % line, file=sys.stderr) def median(values, key=None): if not key: key = None # map and sort accept None as identity length = len(values) if length % 2: median_indeces = [length/2] else: median_indeces = [length/2-1, length/2] values = sorted(values, key=key) return sum(map(key, [values[i] for i in median_indeces])) // len(median_indeces) def test_median(): assert 6 == median([8, 7, 9, 1, 2, 6, 3]) # odd-sized list assert 4 == median([4, 5, 2, 1, 9, 10]) # even-sized int list. (4+5)/2 = 4 # even-sized float list. (4.0+5)/2 = 4.5 assert "4.50" == "%.2f" % median([4.0, 5, 2, 1, 9, 10]) def histogram(stream, options): """ Loop over the stream and add each entry to the dataset, printing out at the end. stream yields Decimal() """ if not options.min or not options.max: # glob the iterator here so we can do min/max on it data = list(stream) else: data = stream bucket_scale = 1 if options.min: min_v = Decimal(options.min) else: min_v = min(data, key=lambda x: x.value) min_v = min_v.value if options.max: max_v = Decimal(options.max) else: max_v = max(data, key=lambda x: x.value) max_v = max_v.value if not max_v > min_v: raise ValueError('max must be > min. max:%s min:%s' % (max_v, min_v)) diff = max_v - min_v boundaries = [] bucket_counts = [] buckets = 0 if options.custbuckets: bound = options.custbuckets.split(',') bound_sort = sorted(map(Decimal, bound)) # if the last value is smaller than the maximum, replace it if bound_sort[-1] < max_v: bound_sort[-1] = max_v # iterate through the sorted list and append to boundaries for x in bound_sort: if x >= min_v and x <= max_v: boundaries.append(x) elif x >= max_v: boundaries.append(max_v) break # beware: the min_v is not included in the boundaries, # so no need to do a -1! bucket_counts = [0 for x in range(len(boundaries))] buckets = len(boundaries) elif options.logscale: buckets = options.buckets and int(options.buckets) or 10 if buckets <= 0: raise ValueError('# of buckets must be > 0') def first_bucket_size(k, n): """Logarithmic buckets means, the size of bucket i+1 is twice the size of bucket i. For k+1 buckets whose sum is n, we have (note, k+1 buckets, since 0 is counted as well): \sum_{i=0}^{k} x*2^i = n x * \sum_{i=0}^{k} 2^i = n x * (2^{k+1} - 1) = n x = n/(2^{k+1} - 1) """ return n/(2**(k+1)-1) def log_steps(k, n): "k logarithmic steps whose sum is n" x = first_bucket_size(k-1, n) sum = 0 for i in range(k): sum += 2**i * x yield sum bucket_counts = [0 for x in range(buckets)] for step in log_steps(buckets, diff): boundaries.append(min_v + step) else: buckets = options.buckets and int(options.buckets) or 10 if buckets <= 0: raise ValueError('# of buckets must be > 0') step = diff // buckets bucket_counts = [0 for x in range(buckets)] for x in range(buckets): boundaries.append(min_v + (step * (x + 1))) skipped = 0 samples = 0 mvsd = MVSD() accepted_data = [] for record in data: samples += record.count if options.mvsd: mvsd.add(record.value, record.count) accepted_data.append(record) # find the bucket this goes in if record.value < min_v or record.value > max_v: skipped += record.count continue for bucket_postion, boundary in enumerate(boundaries): if record.value <= boundary: bucket_counts[bucket_postion] += record.count break # auto-pick the hash scale if max(bucket_counts) > 70: bucket_scale = int(max(bucket_counts) // 70) # print("# NumSamples = %d; Min = %0.2f; Max = %0.2f" % # (samples, min_v, max_v)) # if skipped: # print("# %d value%s outside of min/max" % # (skipped, skipped > 1 and 's' or '')) # if options.mvsd: # print("# Mean = %f; Variance = %f; SD = %f; Median %f" % # (mvsd.mean(), mvsd.var(), mvsd.sd(), # median(accepted_data, key=lambda x: x.value))) bucket_scale_str = "{:,}".format(bucket_scale) print("Each " + options.dot + " represents %s bases" % bucket_scale_str) bucket_min = min_v bucket_max = min_v percentage = "" # Allocate however many characters to the bucket count formed as are needed # for the largest value. largest_bucket_count = max(bucket_counts[i] for i in range(buckets)) bucket_count_chars = len("{:,}".format(largest_bucket_count)) bucket_count_format = "{:" + str(bucket_count_chars) + ",}" # If the largest bucket is large, then assume we're looking at read lengths # and format bucket ranges as integers. If it's small, assume we're looking # at qualitys and format the ranges with decimals. largest_bucket = max(boundaries[i] for i in range(buckets)) if largest_bucket >= 1000: largest_bucket_chars = len("%.0f" % largest_bucket) bucket_format = "%" + str(largest_bucket_chars) + ".0f" else: largest_bucket_chars = len("%.1f" % largest_bucket) bucket_format = "%" + str(largest_bucket_chars) + ".1f" format_string = bucket_format + ' - ' + bucket_format + ' [%s]: %s%s' for bucket in range(buckets): bucket_min = bucket_max bucket_max = boundaries[bucket] bucket_count = bucket_counts[bucket] bucket_count_str = bucket_count_format.format(bucket_count) star_count = 0 if bucket_count: star_count = bucket_count // bucket_scale if options.percentage: percentage = " (%0.2f%%)" % (100 * Decimal(bucket_count) / Decimal(samples)) print(format_string % (bucket_min, bucket_max, bucket_count_str, options.dot * star_count, percentage)) if __name__ == "__main__": parser = OptionParser() parser.usage = "cat data | %prog [options]" parser.add_option("-a", "--agg", dest="agg_value_key", default=False, action="store_true", help="Two column input format, " + "space seperated with valuekey") parser.add_option("-A", "--agg-key-value", dest="agg_key_value", default=False, action="store_true", help="Two column " + "input format, space seperated with keyvalue") parser.add_option("-m", "--min", dest="min", help="minimum value for graph") parser.add_option("-x", "--max", dest="max", help="maximum value for graph") parser.add_option("-b", "--buckets", dest="buckets", help="Number of buckets to use for the histogram") parser.add_option("-l", "--logscale", dest="logscale", default=False, action="store_true", help="Buckets grow in logarithmic scale") parser.add_option("-B", "--custom-buckets", dest="custbuckets", help="Comma seperated list of bucket " + "edges for the histogram") parser.add_option("--no-mvsd", dest="mvsd", action="store_false", default=True, help="Disable the calculation of Mean, " + "Variance and SD (improves performance)") parser.add_option("-f", "--bucket-format", dest="format", default="%10.2f", help="format for bucket numbers") parser.add_option("-p", "--percentage", dest="percentage", default=False, action="store_true", help="List percentage for each bar") parser.add_option("--dot", dest="dot", default='∎', help="Dot representation") (options, args) = parser.parse_args() if sys.stdin.isatty(): # if isatty() that means it's run without anything piped into it parser.print_usage() print("for more help use --help") sys.exit(1) histogram(load_stream(sys.stdin, options.agg_value_key, options.agg_key_value), options) Filtlong-0.2.1/scripts/read_info_histograms.sh000077500000000000000000000030071406722713100215070ustar00rootroot00000000000000#!/usr/bin/env bash long_reads=$1 illumina_1=$2 illumina_2=$3 # Get the directory of this script. script_dir="$( cd "$( dirname "${BASH_SOURCE[0]}" )" && pwd )" # Get paths to other necessary files. hist="$script_dir"/histogram.py filtlong="$script_dir"/../bin/filtlong # Prepare a tab-delimited file of read info. if [ -n "$illumina_1" ] && [ -n "$illumina_2" ]; then illumina_args="-1 "$illumina_1" -2 "$illumina_2 else illumina_args="" fi $filtlong $illumina_args --min_mean_q 1 --verbose $long_reads 2>&1 >/dev/null | grep -P "length = \d+" | sed 's/\s\+length = //' | sed 's/\s\+\w\+ quality = /\t/g' > temp_filtlong_read_info printf "\n" echo "READ SET SUMMARY" echo "----------------" printf "number of reads: %'d\n" $(cat temp_filtlong_read_info | wc -l) total_bases=$(cut -f1 temp_filtlong_read_info | paste -sd+ - | bc) printf "number of bases: %'d\n" $total_bases target=$(($total_bases / 2)) n50=$(zless $long_reads | paste - - - - | cut -f2 | awk '{print length($0);}' | sort -nr | awk -v tar="$target" '{sum += $0; if (sum > tar) {print($0); exit;}}') printf "N50 read length: %'d\n" $n50 printf "\n\n" echo "READ LENGTHS" echo "------------" cat temp_filtlong_read_info | awk '{print $1, $1}' | $hist -a -m 0 -b 40 printf "\n\n" echo "MEAN QUALITIES" echo "--------------" cat temp_filtlong_read_info | awk '{print $1, $2}' | $hist -a -b 40 printf "\n\n" echo "WINDOW QUALITIES" echo "----------------" cat temp_filtlong_read_info | awk '{print $1, $3}' | $hist -a -b 40 printf "\n" rm temp_filtlong_read_info Filtlong-0.2.1/src/000077500000000000000000000000001406722713100140625ustar00rootroot00000000000000Filtlong-0.2.1/src/args.h000066400000000000000000002431361406722713100152000ustar00rootroot00000000000000/* Copyright (c) 2016 Taylor C. Richberger * * Permission is hereby granted, free of charge, to any person obtaining a copy * of this software and associated documentation files (the "Software"), to * deal in the Software without restriction, including without limitation the * rights to use, copy, modify, merge, publish, distribute, sublicense, and/or * sell copies of the Software, and to permit persons to whom the Software is * furnished to do so, subject to the following conditions: * * The above copyright notice and this permission notice shall be included in * all copies or substantial portions of the Software. * * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS * IN THE SOFTWARE. */ /** \file args.hxx * \brief this single-header lets you use all of the args functionality * * The important stuff is done inside the args namespace */ #ifndef ARGS_HXX #define ARGS_HXX #include #include #include #include #include #include #include #include #include #include #ifdef ARGS_TESTNAMESPACE namespace argstest { #else /** \namespace args * \brief contains all the functionality of the args library */ namespace args { #endif /** Getter to grab the value from the argument type. * * If the Get() function of the type returns a reference, so does this, and * the value will be modifiable. */ template auto get(Option &option_) -> decltype(option_.Get()) { return option_.Get(); } /** (INTERNAL) Count UTF-8 glyphs * * This is not reliable, and will fail for combinatory glyphs, but it's * good enough here for now. * * \param string The string to count glyphs from * \return The UTF-8 glyphs in the string */ std::string::size_type Glyphs(const std::string &string_) { std::string::size_type length = 0; for (const char c: string_) { if ((c & 0xc0) != 0x80) { ++length; } } return length; } /** (INTERNAL) Wrap a string into a vector of lines * * This is quick and hacky, but works well enough. You can specify a * different width for the first line * * \param width The width of the body * \param the widtho f the first line, defaults to the width of the body * \return the vector of lines */ std::vector Wrap(const std::string &in, const std::string::size_type width, std::string::size_type firstlinewidth = 0) { // Preserve existing line breaks const auto newlineloc = in.find('\n'); if (newlineloc != in.npos) { auto first = Wrap(std::string(in, 0, newlineloc), width); auto second = Wrap(std::string(in, newlineloc + 1), width); first.insert( std::end(first), std::make_move_iterator(std::begin(second)), std::make_move_iterator(std::end(second))); return first; } if (firstlinewidth == 0) { firstlinewidth = width; } auto currentwidth = firstlinewidth; std::istringstream stream(in); std::vector output; std::ostringstream line; std::string::size_type linesize = 0; while (stream) { std::string item; stream >> item; auto itemsize = Glyphs(item); if ((linesize + 1 + itemsize) > currentwidth) { if (linesize > 0) { output.push_back(line.str()); line.str(std::string()); linesize = 0; currentwidth = width; } } if (itemsize > 0) { if (linesize) { ++linesize; line << " "; } line << item; linesize += itemsize; } } if (linesize > 0) { output.push_back(line.str()); } return output; } #ifdef ARGS_NOEXCEPT /// Error class, for when ARGS_NOEXCEPT is defined enum class Error { None, Usage, Parse, Validation, Map, Extra, Help }; #else /** Base error class */ class Error : public std::runtime_error { public: Error(const std::string &problem) : std::runtime_error(problem) {} virtual ~Error() {}; }; /** Errors that occur during usage */ class UsageError : public Error { public: UsageError(const std::string &problem) : Error(problem) {} virtual ~UsageError() {}; }; /** Errors that occur during regular parsing */ class ParseError : public Error { public: ParseError(const std::string &problem) : Error(problem) {} virtual ~ParseError() {}; }; /** Errors that are detected from group validation after parsing finishes */ class ValidationError : public Error { public: ValidationError(const std::string &problem) : Error(problem) {} virtual ~ValidationError() {}; }; /** Errors in map lookups */ class MapError : public ParseError { public: MapError(const std::string &problem) : ParseError(problem) {} virtual ~MapError() {}; }; /** Error that occurs when a singular flag is specified multiple times */ class ExtraError : public ParseError { public: ExtraError(const std::string &problem) : ParseError(problem) {} virtual ~ExtraError() {}; }; /** An exception that indicates that the user has requested help */ class Help : public Error { public: Help(const std::string &flag) : Error(flag) {} virtual ~Help() {}; }; #endif /** A simple unified option type for unified initializer lists for the Matcher class. */ struct EitherFlag { const bool isShort; const char shortFlag; const std::string longFlag; EitherFlag(const std::string &flag) : isShort(false), shortFlag(), longFlag(flag) {} EitherFlag(const char *flag) : isShort(false), shortFlag(), longFlag(flag) {} EitherFlag(const char flag) : isShort(true), shortFlag(flag), longFlag() {} /** Get just the long flags from an initializer list of EitherFlags */ static std::unordered_set GetLong(std::initializer_list flags) { std::unordered_set longFlags; for (const EitherFlag &flag: flags) { if (!flag.isShort) { longFlags.insert(flag.longFlag); } } return longFlags; } /** Get just the short flags from an initializer list of EitherFlags */ static std::unordered_set GetShort(std::initializer_list flags) { std::unordered_set shortFlags; for (const EitherFlag &flag: flags) { if (flag.isShort) { shortFlags.insert(flag.shortFlag); } } return shortFlags; } }; /** A class of "matchers", specifying short and flags that can possibly be * matched. * * This is supposed to be constructed and then passed in, not used directly * from user code. */ class Matcher { private: const std::unordered_set shortFlags; const std::unordered_set longFlags; public: /** Specify short and long flags separately as iterators * * ex: `args::Matcher(shortFlags.begin(), shortFlags.end(), longFlags.begin(), longFlags.end())` */ template Matcher(ShortIt shortFlagsStart, ShortIt shortFlagsEnd, LongIt longFlagsStart, LongIt longFlagsEnd) : shortFlags(shortFlagsStart, shortFlagsEnd), longFlags(longFlagsStart, longFlagsEnd) {} /** Specify short and long flags separately as iterables * * ex: `args::Matcher(shortFlags, longFlags)` */ template Matcher(Short &&shortIn, Long &&longIn) : shortFlags(std::begin(shortIn), std::end(shortIn)), longFlags(std::begin(longIn), std::end(longIn)) {} /** Specify a mixed single initializer-list of both short and long flags * * This is the fancy one. It takes a single initializer list of * any number of any mixed kinds of flags. Chars are * automatically interpreted as short flags, and strings are * automatically interpreted as long flags: * * args::Matcher{'a'} * args::Matcher{"foo"} * args::Matcher{'h', "help"} * args::Matcher{"foo", 'f', 'F', "FoO"} */ Matcher(std::initializer_list in) : shortFlags(EitherFlag::GetShort(in)), longFlags(EitherFlag::GetLong(in)) {} Matcher(Matcher &&other) : shortFlags(std::move(other.shortFlags)), longFlags(std::move(other.longFlags)) {} ~Matcher() {} /** (INTERNAL) Check if there is a match of a short flag */ bool Match(const char flag) const { return shortFlags.find(flag) != shortFlags.end(); } /** (INTERNAL) Check if there is a match of a long flag */ bool Match(const std::string &flag) const { return longFlags.find(flag) != longFlags.end(); } /** (INTERNAL) Get all flag strings as a vector, with the prefixes embedded */ std::vector GetFlagStrings(const std::string &shortPrefix, const std::string &longPrefix) const { std::vector flagStrings; flagStrings.reserve(shortFlags.size() + longFlags.size()); for (const char flag: shortFlags) { flagStrings.emplace_back(shortPrefix + std::string(1, flag)); } for (const std::string &flag: longFlags) { flagStrings.emplace_back(longPrefix + flag); } return flagStrings; } /** (INTERNAL) Get all flag strings as a vector, with the prefixes and names embedded */ std::vector GetFlagStrings(const std::string &shortPrefix, const std::string &longPrefix, const std::string &name, const std::string &shortSeparator, const std::string longSeparator) const { const std::string bracedname(std::string("[") + name + "]"); std::vector flagStrings; flagStrings.reserve(shortFlags.size() + longFlags.size()); for (const char flag: shortFlags) { flagStrings.emplace_back(shortPrefix + std::string(1, flag) + shortSeparator + bracedname); } for (const std::string &flag: longFlags) { flagStrings.emplace_back(longPrefix + flag + longSeparator + bracedname); } return flagStrings; } }; /** Base class for all match types */ class Base { protected: bool matched; const std::string help; #ifdef ARGS_NOEXCEPT /// Only for ARGS_NOEXCEPT Error error; #endif public: Base(const std::string &help_) : matched(false), help(help_) {} virtual ~Base() {} virtual bool Matched() const noexcept { return matched; } operator bool() const noexcept { return Matched(); } virtual std::tuple GetDescription(const std::string &, const std::string &, const std::string &, const std::string &) const { std::tuple description; std::get<1>(description) = help; return description; } virtual void Reset() noexcept { matched = false; #ifdef ARGS_NOEXCEPT error = Error::None; #endif } #ifdef ARGS_NOEXCEPT /// Only for ARGS_NOEXCEPT virtual Error GetError() const { return error; } #endif }; /** Base class for all match types that have a name */ class NamedBase : public Base { protected: const std::string name; bool kickout; public: NamedBase(const std::string &name_, const std::string &help_) : Base(help_), name(name_), kickout(false) {} virtual ~NamedBase() {} virtual std::tuple GetDescription(const std::string &, const std::string &, const std::string &, const std::string &) const override { std::tuple description; std::get<0>(description) = Name(); std::get<1>(description) = help; return description; } virtual std::string Name() const { return name; } /// Sets a kick-out value for building subparsers void KickOut(bool kickout_) noexcept { this->kickout = kickout_; } /// Gets the kick-out value for building subparsers bool KickOut() const noexcept { return kickout; } }; /** Base class for all flag options */ class FlagBase : public NamedBase { private: const bool extraError; protected: const Matcher matcher; public: FlagBase(const std::string &name_, const std::string &help_, Matcher &&matcher_, const bool extraError_ = false) : NamedBase(name_, help_), extraError(extraError_), matcher(std::move(matcher_)) {} virtual ~FlagBase() {} virtual FlagBase *Match(const std::string &flag) { if (matcher.Match(flag)) { if (extraError && matched) { #ifdef ARGS_NOEXCEPT error = Error::Extra; #else std::ostringstream problem; problem << "Error: flag '" << flag << "' was passed multiple times, but is only allowed to be passed once"; throw ExtraError(problem.str()); #endif } matched = true; return this; } return nullptr; } virtual FlagBase *Match(const char flag) { if (matcher.Match(flag)) { if (extraError && matched) { #ifdef ARGS_NOEXCEPT error = Error::Extra; #else std::ostringstream problem; problem << "Error: flag '" << flag << "' was passed multiple times, but is only allowed to be passed once"; throw ExtraError(problem.str()); #endif } matched = true; return this; } return nullptr; } virtual std::tuple GetDescription(const std::string &shortPrefix, const std::string &longPrefix, const std::string &, const std::string &) const override { std::tuple description; const auto flagStrings = matcher.GetFlagStrings(shortPrefix, longPrefix); std::ostringstream flagstream; for (auto it = std::begin(flagStrings); it != std::end(flagStrings); ++it) { if (it != std::begin(flagStrings)) { flagstream << ", "; } flagstream << *it; } std::get<0>(description) = flagstream.str(); std::get<1>(description) = help; return description; } }; /** Base class for value-accepting flag options */ class ValueFlagBase : public FlagBase { public: ValueFlagBase(const std::string &name_, const std::string &help_, Matcher &&matcher_, const bool extraError_ = false) : FlagBase(name_, help_, std::move(matcher_), extraError_) {} virtual ~ValueFlagBase() {} virtual void ParseValue(const std::string &value) = 0; virtual std::tuple GetDescription(const std::string &shortPrefix, const std::string &longPrefix, const std::string &shortSeparator, const std::string &longSeparator) const override { std::tuple description; const auto flagStrings = matcher.GetFlagStrings(shortPrefix, longPrefix, Name(), shortSeparator, longSeparator); std::ostringstream flagstream; for (auto it = std::begin(flagStrings); it != std::end(flagStrings); ++it) { if (it != std::begin(flagStrings)) { flagstream << ", "; } flagstream << *it; } std::get<0>(description) = flagstream.str(); std::get<1>(description) = help; return description; } }; /** Base class for positional options */ class PositionalBase : public NamedBase { protected: bool ready; public: PositionalBase(const std::string &name_, const std::string &help_) : NamedBase(name_, help_), ready(true) {} virtual ~PositionalBase() {} bool Ready() { return ready; } virtual void ParseValue(const std::string &value_) = 0; virtual void Reset() noexcept override { matched = false; ready = true; #ifdef ARGS_NOEXCEPT error = Error::None; #endif } }; /** Class for all kinds of validating groups, including ArgumentParser */ class Group : public Base { private: std::vector children; std::function validator; public: /** Default validators */ struct Validators { static bool Xor(const Group &group) { return group.MatchedChildren() == 1; } static bool AtLeastOne(const Group &group) { return group.MatchedChildren() >= 1; } static bool AtMostOne(const Group &group) { return group.MatchedChildren() <= 1; } static bool All(const Group &group) { return group.Children().size() == group.MatchedChildren(); } static bool AllOrNone(const Group &group) { return (All(group) || None(group)); } static bool AllChildGroups(const Group &group) { return std::find_if(std::begin(group.Children()), std::end(group.Children()), [](const Base* child) -> bool { return dynamic_cast(child) && !child->Matched(); }) == std::end(group.Children()); } static bool DontCare(const Group &) { return true; } static bool CareTooMuch(const Group &) { return false; } static bool None(const Group &group) { return group.MatchedChildren() == 0; } }; /// If help is empty, this group will not be printed in help output Group(const std::string &help_ = std::string(), const std::function &validator_ = Validators::DontCare) : Base(help_), validator(validator_) {} /// If help is empty, this group will not be printed in help output Group(Group &group_, const std::string &help_ = std::string(), const std::function &validator_ = Validators::DontCare) : Base(help_), validator(validator_) { group_.Add(*this); } virtual ~Group() {} /** Return the first FlagBase that matches flag, or nullptr * * \param flag The flag with prefixes stripped * \return the first matching FlagBase pointer, or nullptr if there is no match */ template FlagBase *Match(const T &flag) { for (Base *child: children) { if (FlagBase *flagBase = dynamic_cast(child)) { if (FlagBase *match = flagBase->Match(flag)) { return match; } } else if (Group *group = dynamic_cast(child)) { if (FlagBase *match = group->Match(flag)) { return match; } } } return nullptr; } /** Get the next ready positional, or nullptr if there is none * * \return the first ready PositionalBase pointer, or nullptr if there is no match */ PositionalBase *GetNextPositional() { for (Base *child: children) { auto next = dynamic_cast(child); auto group = dynamic_cast(child); if (group) { next = group->GetNextPositional(); } if (next && next->Ready()) { return next; } } return nullptr; } /** Get whether this has any FlagBase children * * \return Whether or not there are any FlagBase children */ bool HasFlag() const { for (Base *child: children) { if (dynamic_cast(child)) { return true; } if (auto group = dynamic_cast(child)) { if (group->HasFlag()) { return true; } } } return false; } /** Append a child to this Group. */ void Add(Base &child) { children.emplace_back(&child); } /** Get all this group's children */ const std::vector &Children() const { return children; } /** Count the number of matched children this group has */ std::vector::size_type MatchedChildren() const { return std::count_if(std::begin(children), std::end(children), [](const Base *child){return child->Matched();}); } /** Whether or not this group matches validation */ virtual bool Matched() const noexcept override { return validator(*this); } /** Get validation */ bool Get() const { return Matched(); } /** Get all the child descriptions for help generation */ std::vector> GetChildDescriptions(const std::string &shortPrefix, const std::string &longPrefix, const std::string &shortSeparator, const std::string &longSeparator, const unsigned int indent = 0) const { std::vector> descriptions; for (const auto &child: children) { if (const auto group = dynamic_cast(child)) { // Push that group description on the back if not empty unsigned char addindent = 0; if (!group->help.empty()) { descriptions.emplace_back(group->help, "", indent); addindent = 1; } auto groupDescriptions = group->GetChildDescriptions(shortPrefix, longPrefix, shortSeparator, longSeparator, indent + addindent); descriptions.insert( std::end(descriptions), std::make_move_iterator(std::begin(groupDescriptions)), std::make_move_iterator(std::end(groupDescriptions))); } else if (const auto named = dynamic_cast(child)) { const auto description = named->GetDescription(shortPrefix, longPrefix, shortSeparator, longSeparator); descriptions.emplace_back(std::get<0>(description), std::get<1>(description), indent); } } return descriptions; } std::vector> GetChildDescriptionsPositional(const std::string &shortPrefix, const std::string &longPrefix, const std::string &shortSeparator, const std::string &longSeparator, const unsigned int indent = 0) const { std::vector> descriptions; for (const auto &child: children) { if (const auto named = dynamic_cast(child)) { const auto description = named->GetDescription(shortPrefix, longPrefix, shortSeparator, longSeparator); descriptions.emplace_back(std::get<0>(description), std::get<1>(description), indent); } } return descriptions; } /** Get the names of positional parameters */ std::vector GetPosNames() const { std::vector names; for (const auto &child: children) { if (const Group *group = dynamic_cast(child)) { auto groupNames = group->GetPosNames(); names.insert( std::end(names), std::make_move_iterator(std::begin(groupNames)), std::make_move_iterator(std::end(groupNames))); } else if (const PositionalBase *pos = dynamic_cast(child)) { names.emplace_back(pos->Name()); } } return names; } virtual void Reset() noexcept override { for (auto &child: children) { child->Reset(); } #ifdef ARGS_NOEXCEPT error = Error::None; #endif } #ifdef ARGS_NOEXCEPT /// Only for ARGS_NOEXCEPT virtual Error GetError() const override { if (error != Error::None) { return error; } auto it = std::find_if(std::begin(children), std::end(children), [](const Base *child){return child->GetError() != Error::None;}); if (it == std::end(children)) { return Error::None; } else { return (*it)->GetError(); } } #endif }; /** The main user facing command line argument parser class */ class ArgumentParser : public Group { private: std::string prog; std::string proglinePostfix; std::string description; std::string epilog; std::string longprefix; std::string shortprefix; std::string longseparator; std::string terminator; bool allowJoinedShortValue; bool allowJoinedLongValue; bool allowSeparateShortValue; bool allowSeparateLongValue; public: /** A simple structure of parameters for easy user-modifyable help menus */ struct HelpParams { /** The width of the help menu */ unsigned int width = 80; /** The indent of the program line */ unsigned int progindent = 2; /** The indent of the program trailing lines for long parameters */ unsigned int progtailindent = 4; /** The indent of the description and epilogs */ unsigned int descriptionindent = 4; /** The indent of the flags */ unsigned int flagindent = 6; /** The indent of the flag descriptions */ unsigned int helpindent = 40; /** The additional indent each group adds */ unsigned int eachgroupindent = 2; /** The minimum gutter between each flag and its help */ unsigned int gutter = 1; /** Show the terminator when both options and positional parameters are present */ bool showTerminator = true; /** Show the {OPTIONS} on the prog line when this is true */ bool showProglineOptions = true; /** Show the positionals on the prog line when this is true */ bool showProglinePositionals = true; } helpParams; ArgumentParser(const std::string &description_, const std::string &epilog_ = std::string()) : Group("", Group::Validators::AllChildGroups), description(description_), epilog(epilog_), longprefix("--"), shortprefix("-"), longseparator("="), terminator("--"), allowJoinedShortValue(true), allowJoinedLongValue(true), allowSeparateShortValue(true), allowSeparateLongValue(true) {} /** The program name for help generation */ const std::string &Prog() const { return prog; } /** The program name for help generation */ void Prog(const std::string &prog_) { this->prog = prog_; } /** The description that appears on the prog line after options */ const std::string &ProglinePostfix() const { return proglinePostfix; } /** The description that appears on the prog line after options */ void ProglinePostfix(const std::string &proglinePostfix_) { this->proglinePostfix = proglinePostfix_; } /** The description that appears above options */ const std::string &Description() const { return description; } /** The description that appears above options */ void Description(const std::string &description_) { this->description = description_; } /** The description that appears below options */ const std::string &Epilog() const { return epilog; } /** The description that appears below options */ void Epilog(const std::string &epilog_) { this->epilog = epilog_; } /** The prefix for long flags */ const std::string &LongPrefix() const { return longprefix; } /** The prefix for long flags */ void LongPrefix(const std::string &longprefix_) { this->longprefix = longprefix_; } /** The prefix for short flags */ const std::string &ShortPrefix() const { return shortprefix; } /** The prefix for short flags */ void ShortPrefix(const std::string &shortprefix_) { this->shortprefix = shortprefix_; } /** The separator for long flags */ const std::string &LongSeparator() const { return longseparator; } /** The separator for long flags */ void LongSeparator(const std::string &longseparator_) { if (longseparator_.empty()) { #ifdef ARGS_NOEXCEPT error = Error::Usage; #else throw UsageError("longseparator can not be set to empty"); #endif } else { this->longseparator = longseparator_; } } /** The terminator that forcibly separates flags from positionals */ const std::string &Terminator() const { return terminator; } /** The terminator that forcibly separates flags from positionals */ void Terminator(const std::string &terminator_) { this->terminator = terminator_; } /** Get the current argument separation parameters. * * See SetArgumentSeparations for details on what each one means. */ void GetArgumentSeparations( bool &allowJoinedShortValue_, bool &allowJoinedLongValue_, bool &allowSeparateShortValue_, bool &allowSeparateLongValue_) const { allowJoinedShortValue_ = this->allowJoinedShortValue; allowJoinedLongValue_ = this->allowJoinedLongValue; allowSeparateShortValue_ = this->allowSeparateShortValue; allowSeparateLongValue_ = this->allowSeparateLongValue; } /** Change allowed option separation. * * \param allowJoinedShortValue Allow a short flag that accepts an argument to be passed its argument immediately next to it (ie. in the same argv field) * \param allowJoinedLongValue Allow a long flag that accepts an argument to be passed its argument separated by the longseparator (ie. in the same argv field) * \param allowSeparateShortValue Allow a short flag that accepts an argument to be passed its argument separated by whitespace (ie. in the next argv field) * \param allowSeparateLongValue Allow a long flag that accepts an argument to be passed its argument separated by whitespace (ie. in the next argv field) */ void SetArgumentSeparations( const bool allowJoinedShortValue_, const bool allowJoinedLongValue_, const bool allowSeparateShortValue_, const bool allowSeparateLongValue_) { this->allowJoinedShortValue = allowJoinedShortValue_; this->allowJoinedLongValue = allowJoinedLongValue_; this->allowSeparateShortValue = allowSeparateShortValue_; this->allowSeparateLongValue = allowSeparateLongValue_; } /** Pass the help menu into an ostream */ void Help(std::ostream &help_) const { bool hasoptions = false; bool hasarguments = false; const auto description_text = Wrap(this->description, helpParams.width - helpParams.descriptionindent); const auto epilog_text = Wrap(this->epilog, helpParams.width - helpParams.descriptionindent); std::ostringstream prognameline; prognameline << "usage: "; prognameline << prog; if (HasFlag()) { hasoptions = true; if (helpParams.showProglineOptions) { prognameline << " {OPTIONS}"; } } for (const std::string &posname: GetPosNames()) { hasarguments = true; if (helpParams.showProglinePositionals) { prognameline << " [" << posname << ']'; } } if (!proglinePostfix.empty()) { prognameline << ' ' << proglinePostfix; } const auto proglines = Wrap(prognameline.str(), helpParams.width - (helpParams.progindent + 4), helpParams.width - helpParams.progindent); auto progit = std::begin(proglines); if (progit != std::end(proglines)) { help_ << std::string(helpParams.progindent, ' ') << *progit << '\n'; ++progit; } for (; progit != std::end(proglines); ++progit) { help_ << std::string(helpParams.progtailindent, ' ') << *progit << '\n'; } help_ << '\n'; for (const auto &line: description_text) { help_ << std::string(helpParams.descriptionindent, ' ') << line << "\n"; } help_ << "\n"; help_ << std::string(helpParams.progindent, ' ') << "positional arguments:\n"; for (const auto &desc: GetChildDescriptionsPositional(shortprefix, longprefix, allowJoinedShortValue ? "" : " ", allowJoinedLongValue ? longseparator : " ")) { const auto groupindent = std::get<2>(desc) * helpParams.eachgroupindent; const auto flags = Wrap(std::get<0>(desc), helpParams.width - (helpParams.flagindent + helpParams.helpindent + helpParams.gutter)); const auto info = Wrap(std::get<1>(desc), helpParams.width - (helpParams.helpindent + groupindent)); std::string::size_type flagssize = 0; for (auto flagsit = std::begin(flags); flagsit != std::end(flags); ++flagsit) { if (flagsit != std::begin(flags)) { help_ << '\n'; } help_ << std::string(groupindent + helpParams.flagindent, ' ') << *flagsit; flagssize = Glyphs(*flagsit); } auto infoit = std::begin(info); // groupindent is on both sides of this inequality, and therefore can be removed if ((helpParams.flagindent + flagssize + helpParams.gutter) > helpParams.helpindent || infoit == std::end(info)) { help_ << '\n'; } else { // groupindent is on both sides of the minus sign, and therefore doesn't actually need to be in here help_ << std::string(helpParams.helpindent - (helpParams.flagindent + flagssize), ' ') << *infoit << '\n'; ++infoit; } for (; infoit != std::end(info); ++infoit) { help_ << std::string(groupindent + helpParams.helpindent, ' ') << *infoit << '\n'; } } help_ << "\n"; help_ << std::string(helpParams.progindent, ' ') << "optional arguments:\n"; for (const auto &desc: GetChildDescriptions(shortprefix, longprefix, allowJoinedShortValue ? "" : " ", allowJoinedLongValue ? longseparator : " ")) { const auto groupindent = std::get<2>(desc) * helpParams.eachgroupindent; // RRW: Group descriptions shouldn't wrap as much as other lines. int flag_wrap_width; if (std::get<0>(desc).find("NL") == 0) flag_wrap_width = helpParams.width - (helpParams.flagindent + helpParams.gutter); else flag_wrap_width = helpParams.width - (helpParams.flagindent + helpParams.helpindent + helpParams.gutter); const auto flags = Wrap(std::get<0>(desc), flag_wrap_width); const auto info = Wrap(std::get<1>(desc), helpParams.width - (helpParams.helpindent + groupindent)); std::string::size_type flagssize = 0; for (auto flagsit = std::begin(flags); flagsit != std::end(flags); ++flagsit) { if (flagsit != std::begin(flags)) { help_ << '\n'; } // RRW: I use "NL" as an indication to put a newline before the line. if (flagsit->find("NL") == 0 or flagsit->find("-h") == 0) help_ << '\n'; if (flagsit->find("NL") == 0) { help_ << std::string(groupindent + helpParams.flagindent, ' ') << flagsit->substr(2); flagssize = Glyphs(*flagsit) - 2; } else { help_ << std::string(groupindent + helpParams.flagindent, ' ') << *flagsit; flagssize = Glyphs(*flagsit); } } auto infoit = std::begin(info); // groupindent is on both sides of this inequality, and therefore can be removed if ((helpParams.flagindent + flagssize + helpParams.gutter) > helpParams.helpindent || infoit == std::end(info)) { help_ << '\n'; } else { // groupindent is on both sides of the minus sign, and therefore doesn't actually need to be in here help_ << std::string(helpParams.helpindent - (helpParams.flagindent + flagssize), ' ') << *infoit << '\n'; ++infoit; } for (; infoit != std::end(info); ++infoit) { help_ << std::string(groupindent + helpParams.helpindent, ' ') << *infoit << '\n'; } } if (hasoptions && hasarguments && helpParams.showTerminator) { for (const auto &item: Wrap(std::string("\"") + terminator + "\" can be used to terminate flag options and force all following arguments to be treated as positional options", helpParams.width - helpParams.flagindent)) { help_ << std::string(helpParams.flagindent, ' ') << item << '\n'; } } help_ << "\n"; for (const auto &line: epilog_text) { help_ << std::string(helpParams.descriptionindent, ' ') << line << "\n"; } } /** Generate a help menu as a string. * * \return the help text as a single string */ std::string Help() const { std::ostringstream help_; Help(help_); return help_.str(); } /** Parse all arguments. * * \param begin an iterator to the beginning of the argument list * \param end an iterator to the past-the-end element of the argument list * \return the iterator after the last parsed value. Only useful for kick-out */ template It ParseArgs(It begin, It end) { // Reset all Matched statuses and errors Reset(); bool terminated = false; // Check all arg chunks for (auto it = begin; it != end; ++it) { const auto &chunk = *it; if (!terminated && chunk == terminator) { terminated = true; // If a long arg was found } else if (!terminated && chunk.find(longprefix) == 0 && chunk.size() > longprefix.size()) { const auto argchunk = chunk.substr(longprefix.size()); // Try to separate it, in case of a separator: const auto separator = longseparator.empty() ? argchunk.npos : argchunk.find(longseparator); // If the separator is in the argument, separate it. const auto arg = (separator != argchunk.npos ? std::string(argchunk, 0, separator) : argchunk); if (auto base = Match(arg)) { if (auto argbase = dynamic_cast(base)) { if (separator != argchunk.npos) { if (allowJoinedLongValue) { argbase->ParseValue(argchunk.substr(separator + longseparator.size())); } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' was passed a joined argument, but these are disallowed"; throw ParseError(problem.str()); #endif } } else { ++it; if (it == end) { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' requires an argument but received none"; throw ParseError(problem.str()); #endif } if (allowSeparateLongValue) { argbase->ParseValue(*it); } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' was passed a separate argument, but these are disallowed"; throw ParseError(problem.str()); #endif } } } else if (separator != argchunk.npos) { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: passed an argument into a non-argument flag: " << chunk; throw ParseError(problem.str()); #endif } if (base->KickOut()) { return ++it; } } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag could not be matched: " << arg; throw ParseError(problem.str()); #endif } // Check short args } else if (!terminated && chunk.find(shortprefix) == 0 && chunk.size() > shortprefix.size()) { const auto argchunk = chunk.substr(shortprefix.size()); for (auto argit = std::begin(argchunk); argit != std::end(argchunk); ++argit) { const auto arg = *argit; if (auto base = Match(arg)) { if (auto argbase = dynamic_cast(base)) { const std::string value(++argit, std::end(argchunk)); if (!value.empty()) { if (allowJoinedShortValue) { argbase->ParseValue(value); } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' was passed a joined argument, but these are disallowed"; throw ParseError(problem.str()); #endif } } else { ++it; if (it == end) { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' requires an argument but received none"; throw ParseError(problem.str()); #endif } if (allowSeparateShortValue) { argbase->ParseValue(*it); } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag '" << arg << "' was passed a separate argument, but these are disallowed"; throw ParseError(problem.str()); #endif } } // Because this argchunk is done regardless break; } if (base->KickOut()) { return ++it; } } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: flag could not be matched: '" << arg << "'"; throw ParseError(problem.str()); #endif } } } else { auto pos = GetNextPositional(); if (pos) { pos->ParseValue(chunk); if (pos->KickOut()) { return ++it; } } else { #ifdef ARGS_NOEXCEPT error = Error::Parse; return it; #else std::ostringstream problem; problem << "Error: passed in argument, but no positional arguments were ready to receive it: " << chunk; throw ParseError(problem.str()); #endif } } } if (!Matched()) { #ifdef ARGS_NOEXCEPT error = Error::Validation; #else std::ostringstream problem; problem << "Error: group validation failed somewhere!"; throw ValidationError(problem.str()); #endif } return end; } /** Parse all arguments. * * \param args an iterable of the arguments * \return the iterator after the last parsed value. Only useful for kick-out */ template auto ParseArgs(const T &args) -> decltype(std::begin(args)) { return ParseArgs(std::begin(args), std::end(args)); } /** Convenience function to parse the CLI from argc and argv * * Just assigns the program name and vectorizes arguments for passing into ParseArgs() * * \return whether or not all arguments were parsed. This works for detecting kick-out, but is generally useless as it can't do anything with it. */ bool ParseCLI(const int argc, const char * const * argv) { if (prog.empty()) { prog.assign(argv[0]); } const std::vector args(argv + 1, argv + argc); return ParseArgs(args) == std::end(args); } }; std::ostream &operator<<(std::ostream &os, const ArgumentParser &parser) { parser.Help(os); return os; } /** Boolean argument matcher */ class Flag : public FlagBase { public: Flag(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const bool extraError_ = false): FlagBase(name_, help_, std::move(matcher_), extraError_) { group_.Add(*this); } virtual ~Flag() {} /** Get whether this was matched */ bool Get() const { return Matched(); } }; /** Help flag class * * Works like a regular flag, but throws an instance of Help when it is matched */ class HelpFlag : public Flag { public: HelpFlag(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_): Flag(group_, name_, help_, std::move(matcher_)) {} virtual ~HelpFlag() {} virtual FlagBase *Match(const std::string &arg) override { if (FlagBase::Match(arg)) { #ifdef ARGS_NOEXCEPT error = Error::Help; return this; #else throw Help(arg); #endif } return nullptr; } virtual FlagBase *Match(const char arg) override { if (FlagBase::Match(arg)) { #ifdef ARGS_NOEXCEPT error = Error::Help; return this; #else throw Help(std::string(1, arg)); #endif } return nullptr; } /** Get whether this was matched */ bool Get() const noexcept { return Matched(); } }; /** A flag class that simply counts the number of times it's matched */ class CounterFlag : public Flag { private: const int startcount; int count; public: CounterFlag(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const int startcount_ = 0): Flag(group_, name_, help_, std::move(matcher_)), startcount(startcount_), count(startcount_) {} virtual ~CounterFlag() {} virtual FlagBase *Match(const std::string &arg) override { auto me = FlagBase::Match(arg); if (me) { ++count; } return me; } virtual FlagBase *Match(const char arg) override { auto me = FlagBase::Match(arg); if (me) { ++count; } return me; } /** Get the count */ int &Get() noexcept { return count; } virtual void Reset() noexcept override { FlagBase::Reset(); count = startcount; } }; /** A default Reader class for argument classes * * Simply uses a std::istringstream to read into the destination type, and * raises a ParseError if there are any characters left. */ template struct ValueReader { bool operator ()(const std::string &name, const std::string &value, T &destination) { std::istringstream ss(value); ss >> destination; if (ss.rdbuf()->in_avail() > 0) { #ifdef ARGS_NOEXCEPT return false; #else std::ostringstream problem; problem << "Error: argument '" << name << "' received invalid value type '" << value << "'"; throw ParseError(problem.str()); #endif } return true; } }; /** std::string specialization for ValueReader * * By default, stream extraction into a string splits on white spaces, and * it is more efficient to ust copy a string into the destination. */ template <> struct ValueReader { bool operator()(const std::string &, const std::string &value, std::string &destination) { destination.assign(value); return true; } }; /** An argument-accepting flag class * * \tparam T the type to extract the argument as * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) */ template < typename T, typename Reader = ValueReader> class ValueFlag : public ValueFlagBase { private: T value; Reader reader; public: ValueFlag(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const T &defaultValue_ = T(), const bool extraError_ = false): ValueFlagBase(name_, help_, std::move(matcher_), extraError_), value(defaultValue_) { group_.Add(*this); } virtual ~ValueFlag() {} virtual void ParseValue(const std::string &value_) override { #ifdef ARGS_NOEXCEPT if (!reader(name, value_, this->value)) { error = Error::Parse; } #else reader(name, value_, this->value); #endif } /** Get the value */ T &Get() noexcept { return value; } }; /** An argument-accepting flag class that pushes the found values into a list * * \tparam T the type to extract the argument as * \tparam List the list type that houses the values * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) */ template < typename T, template class List = std::vector, typename Reader = ValueReader> class ValueFlagList : public ValueFlagBase { private: using Container = List; Container values; Reader reader; public: typedef T value_type; typedef typename Container::allocator_type allocator_type; typedef typename Container::pointer pointer; typedef typename Container::const_pointer const_pointer; typedef T& reference; typedef const T& const_reference; typedef typename Container::size_type size_type; typedef typename Container::difference_type difference_type; typedef typename Container::iterator iterator; typedef typename Container::const_iterator const_iterator; typedef std::reverse_iterator reverse_iterator; typedef std::reverse_iterator const_reverse_iterator; ValueFlagList(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const Container &defaultValues_ = Container()): ValueFlagBase(name_, help_, std::move(matcher_)), values(defaultValues_) { group_.Add(*this); } virtual ~ValueFlagList() {} virtual void ParseValue(const std::string &value_) override { T v; #ifdef ARGS_NOEXCEPT if (!reader(name, value_, v)) { error = Error::Parse; } #else reader(name, value_, v); #endif values.insert(std::end(values), v); } /** Get the values */ Container &Get() noexcept { return values; } virtual std::string Name() const override { return name + std::string("..."); } virtual void Reset() noexcept override { ValueFlagBase::Reset(); values.clear(); } iterator begin() noexcept { return values.begin(); } const_iterator begin() const noexcept { return values.begin(); } const_iterator cbegin() const noexcept { return values.cbegin(); } iterator end() noexcept { return values.end(); } const_iterator end() const noexcept { return values.end(); } const_iterator cend() const noexcept { return values.cend(); } }; /** A mapping value flag class * * \tparam K the type to extract the argument as * \tparam T the type to store the result as * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) * \tparam Map The Map type. Should operate like std::map or std::unordered_map */ template < typename K, typename T, typename Reader = ValueReader, template class Map = std::unordered_map> class MapFlag : public ValueFlagBase { private: const Map map; T value; Reader reader; public: MapFlag(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const Map &map_, const T &defaultValue_ = T(), const bool extraError_ = false): ValueFlagBase(name_, help_, std::move(matcher_), extraError_), map(map_), value(defaultValue_) { group_.Add(*this); } virtual ~MapFlag() {} virtual void ParseValue(const std::string &value_) override { K key; #ifdef ARGS_NOEXCEPT if (!reader(name, value_, key)) { error = Error::Parse; } #else reader(name, value_, key); #endif auto it = map.find(key); if (it == std::end(map)) { #ifdef ARGS_NOEXCEPT error = Error::Map; #else std::ostringstream problem; problem << "Error: could not find key '" << key << "' in map for arg '" << name << "'"; throw MapError(problem.str()); #endif } else { this->value = it->second; } } /** Get the value */ T &Get() noexcept { return value; } }; /** A mapping value flag list class * * \tparam K the type to extract the argument as * \tparam T the type to store the result as * \tparam List the list type that houses the values * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) * \tparam Map The Map type. Should operate like std::map or std::unordered_map */ template < typename K, typename T, template class List = std::vector, typename Reader = ValueReader, template class Map = std::unordered_map> class MapFlagList : public ValueFlagBase { private: using Container = List; const Map map; Container values; Reader reader; public: typedef T value_type; typedef typename Container::allocator_type allocator_type; typedef typename Container::pointer pointer; typedef typename Container::const_pointer const_pointer; typedef T& reference; typedef const T& const_reference; typedef typename Container::size_type size_type; typedef typename Container::difference_type difference_type; typedef typename Container::iterator iterator; typedef typename Container::const_iterator const_iterator; typedef std::reverse_iterator reverse_iterator; typedef std::reverse_iterator const_reverse_iterator; MapFlagList(Group &group_, const std::string &name_, const std::string &help_, Matcher &&matcher_, const Map &map_, const Container &defaultValues_ = Container()): ValueFlagBase(name_, help_, std::move(matcher_)), map(map_), values(defaultValues_) { group_.Add(*this); } virtual ~MapFlagList() {} virtual void ParseValue(const std::string &value) override { K key; #ifdef ARGS_NOEXCEPT if (!reader(name, value, key)) { error = Error::Parse; } #else reader(name, value, key); #endif auto it = map.find(key); if (it == std::end(map)) { #ifdef ARGS_NOEXCEPT error = Error::Map; #else std::ostringstream problem; problem << "Error: could not find key '" << key << "' in map for arg '" << name << "'"; throw MapError(problem.str()); #endif } else { this->values.emplace_back(it->second); } } /** Get the value */ Container &Get() noexcept { return values; } virtual std::string Name() const override { return name + std::string("..."); } virtual void Reset() noexcept override { ValueFlagBase::Reset(); values.clear(); } iterator begin() noexcept { return values.begin(); } const_iterator begin() const noexcept { return values.begin(); } const_iterator cbegin() const noexcept { return values.cbegin(); } iterator end() noexcept { return values.end(); } const_iterator end() const noexcept { return values.end(); } const_iterator cend() const noexcept { return values.cend(); } }; /** A positional argument class * * \tparam T the type to extract the argument as * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) */ template < typename T, typename Reader = ValueReader> class Positional : public PositionalBase { private: T value; Reader reader; public: Positional(Group &group_, const std::string &name_, const std::string &help_, const T &defaultValue_ = T()): PositionalBase(name_, help_), value(defaultValue_) { group_.Add(*this); } virtual ~Positional() {} virtual void ParseValue(const std::string &value_) override { #ifdef ARGS_NOEXCEPT if (!reader(name, value_, this->value)) { error = Error::Parse; } #else reader(name, value_, this->value); #endif ready = false; matched = true; } /** Get the value */ T &Get() noexcept { return value; } }; /** A positional argument class that pushes the found values into a list * * \tparam T the type to extract the argument as * \tparam List the list type that houses the values * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) */ template < typename T, template class List = std::vector, typename Reader = ValueReader> class PositionalList : public PositionalBase { private: using Container = List; Container values; Reader reader; public: typedef T value_type; typedef typename Container::allocator_type allocator_type; typedef typename Container::pointer pointer; typedef typename Container::const_pointer const_pointer; typedef T& reference; typedef const T& const_reference; typedef typename Container::size_type size_type; typedef typename Container::difference_type difference_type; typedef typename Container::iterator iterator; typedef typename Container::const_iterator const_iterator; typedef std::reverse_iterator reverse_iterator; typedef std::reverse_iterator const_reverse_iterator; PositionalList(Group &group_, const std::string &name_, const std::string &help_, const Container &defaultValues_ = Container()): PositionalBase(name_, help_), values(defaultValues_) { group_.Add(*this); } virtual ~PositionalList() {} virtual void ParseValue(const std::string &value_) override { T v; #ifdef ARGS_NOEXCEPT if (!reader(name, value_, v)) { error = Error::Parse; } #else reader(name, value_, v); #endif values.insert(std::end(values), v); matched = true; } virtual std::string Name() const override { return name + std::string("..."); } /** Get the values */ Container &Get() noexcept { return values; } virtual void Reset() noexcept override { PositionalBase::Reset(); values.clear(); } iterator begin() noexcept { return values.begin(); } const_iterator begin() const noexcept { return values.begin(); } const_iterator cbegin() const noexcept { return values.cbegin(); } iterator end() noexcept { return values.end(); } const_iterator end() const noexcept { return values.end(); } const_iterator cend() const noexcept { return values.cend(); } }; /** A positional argument mapping class * * \tparam K the type to extract the argument as * \tparam T the type to store the result as * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) * \tparam Map The Map type. Should operate like std::map or std::unordered_map */ template < typename K, typename T, typename Reader = ValueReader, template class Map = std::unordered_map> class MapPositional : public PositionalBase { private: const Map map; T value; Reader reader; public: MapPositional(Group &group_, const std::string &name_, const std::string &help_, const Map &map_, const T &defaultValue_ = T()): PositionalBase(name_, help_), map(map_), value(defaultValue_) { group_.Add(*this); } virtual ~MapPositional() {} virtual void ParseValue(const std::string &value_) override { K key; #ifdef ARGS_NOEXCEPT if (!reader(name, value_, key)) { error = Error::Parse; } #else reader(name, value_, key); #endif auto it = map.find(key); if (it == std::end(map)) { #ifdef ARGS_NOEXCEPT error = Error::Map; #else std::ostringstream problem; problem << "Error: could not find key '" << key << "' in map for arg '" << name << "'"; throw MapError(problem.str()); #endif } else { this->value = it->second; ready = false; matched = true; } } /** Get the value */ T &Get() noexcept { return value; } }; /** A positional argument mapping list class * * \tparam K the type to extract the argument as * \tparam T the type to store the result as * \tparam List the list type that houses the values * \tparam Reader The functor type used to read the argument, taking the name, value, and destination reference with operator(), and returning a bool (if ARGS_NOEXCEPT is defined) * \tparam Map The Map type. Should operate like std::map or std::unordered_map */ template < typename K, typename T, template class List = std::vector, typename Reader = ValueReader, template class Map = std::unordered_map> class MapPositionalList : public PositionalBase { private: using Container = List; const Map map; Container values; Reader reader; public: typedef T value_type; typedef typename Container::allocator_type allocator_type; typedef typename Container::pointer pointer; typedef typename Container::const_pointer const_pointer; typedef T& reference; typedef const T& const_reference; typedef typename Container::size_type size_type; typedef typename Container::difference_type difference_type; typedef typename Container::iterator iterator; typedef typename Container::const_iterator const_iterator; typedef std::reverse_iterator reverse_iterator; typedef std::reverse_iterator const_reverse_iterator; MapPositionalList(Group &group_, const std::string &name_, const std::string &help_, const Map &map_, const Container &defaultValues_ = Container()): PositionalBase(name_, help_), map(map_), values(defaultValues_) { group_.Add(*this); } virtual ~MapPositionalList() {} virtual void ParseValue(const std::string &value_) override { K key; #ifdef ARGS_NOEXCEPT if (!reader(name, value_, key)) { error = Error::Parse; } #else reader(name, value_, key); #endif auto it = map.find(key); if (it == std::end(map)) { #ifdef ARGS_NOEXCEPT error = Error::Map; #else std::ostringstream problem; problem << "Error: could not find key '" << key << "' in map for arg '" << name << "'"; throw MapError(problem.str()); #endif } else { this->values.emplace_back(it->second); matched = true; } } /** Get the value */ Container &Get() noexcept { return values; } virtual std::string Name() const override { return name + std::string("..."); } virtual void Reset() noexcept override { PositionalBase::Reset(); values.clear(); } iterator begin() noexcept { return values.begin(); } const_iterator begin() const noexcept { return values.begin(); } const_iterator cbegin() const noexcept { return values.cbegin(); } iterator end() noexcept { return values.end(); } const_iterator end() const noexcept { return values.end(); } const_iterator cend() const noexcept { return values.cend(); } }; } #endif Filtlong-0.2.1/src/arguments.cpp000066400000000000000000000274161406722713100166050ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #include "arguments.h" #include #include #include #include #include #include "args.h" struct DoublesReader { void operator()(const std::string &name, const std::string &value, double &destination) { try { if (value.find_first_not_of("0123456789.") != std::string::npos) throw std::invalid_argument(""); destination = std::stod(value); } catch ( ... ) { std::ostringstream problem; problem << "Error: argument '" << name << "' received invalid value type '" << value << "'"; throw args::ParseError(problem.str()); } } }; typedef args::ValueFlag d_arg; typedef args::ValueFlag i_arg; typedef args::ValueFlag s_arg; typedef args::Flag f_arg; Arguments::Arguments(int argc, char **argv) { args::ArgumentParser parser("Filtlong: a quality filtering tool for Nanopore and PacBio reads", "For more information, go to: https://github.com/rrwick/Filtlong"); parser.LongSeparator(" "); struct winsize w; ioctl(STDOUT_FILENO, TIOCGWINSZ, &w); int terminal_width = w.ws_col; int indent_size; if (terminal_width > 120) indent_size = 4; else if (terminal_width > 80) indent_size = 3; else if (terminal_width > 60) indent_size = 2; else indent_size = 1; parser.helpParams.showTerminator = false; parser.helpParams.progindent = 0; parser.helpParams.descriptionindent = 0; parser.helpParams.width = terminal_width; parser.helpParams.flagindent = indent_size; parser.helpParams.eachgroupindent = indent_size; args::Positional input_reads_arg(parser, "input_reads", "input long reads to be filtered"); args::Group thresholds_group(parser, "output thresholds:"); i_arg target_bases_arg(thresholds_group, "int", "keep only the best reads up to this many total bases", {'t', "target_bases"}); d_arg keep_percent_arg(thresholds_group, "float", "keep only this percentage of the best reads (measured by bases)", {'p', "keep_percent"}); i_arg min_length_arg(thresholds_group, "int", "minimum length threshold", {"min_length"}); i_arg max_length_arg(thresholds_group, "int", "maximum length threshold", {"max_length"}); d_arg min_mean_q_arg(thresholds_group, "float", "minimum mean quality threshold", {"min_mean_q"}); d_arg min_window_q_arg(thresholds_group, "float", "minimum window quality threshold", {"min_window_q"}); args::Group references_group(parser, "NLexternal references " // The NL at the start results in a newline "(if provided, read quality will be determined using these instead of from the Phred scores):"); s_arg assembly_arg(references_group, "file", "reference assembly in FASTA format", {'a', "assembly"}); s_arg illumina_1_arg(references_group, "file", "reference Illumina reads in FASTQ format", {'1', "illumina_1"}); s_arg illumina_2_arg(references_group, "file", "reference Illumina reads in FASTQ format", {'2', "illumina_2"}); args::Group score_weights_group(parser, "NLscore weights " // The NL at the start results in a newline "(control the relative contribution of each score to the final read score):"); d_arg length_weight_arg(score_weights_group, "float", "weight given to the length score (default: 1)", {"length_weight"}, 1.0); d_arg mean_q_weight_arg(score_weights_group, "float", "weight given to the mean quality score (default: 1)", {"mean_q_weight"}, 1.0); d_arg window_q_weight_arg(score_weights_group, "float", "weight given to the window quality score (default: 1)", {"window_q_weight"}, 1.0); // TO DO: half_length_score: reads of this length get a 50% score (currently hard-coded at 5000) args::Group manipulation_group(parser, "NLread manipulation:"); // The NL at the start results in a newline f_arg trim_arg(manipulation_group, "trim", "trim non-k-mer-matching bases from start/end of reads", {"trim"}); i_arg split_arg(manipulation_group, "split", "split reads at this many (or more) consecutive non-k-mer-matching bases", {"split"}); args::Group other_group(parser, "NLother:"); // The NL at the start results in a newline i_arg window_size_arg(other_group, "int", "size of sliding window used when measuring window quality (default: 250)", {"window_size"}, 250); f_arg verbose_arg(other_group, "verbose", "verbose output to stderr with info for each read", {"verbose"}); f_arg version_arg(other_group, "version", "display the program version and quit", {"version"}); args::HelpFlag help(parser, "help", "display this help menu", {'h', "help"}); parsing_result = GOOD; try { parser.ParseCLI(argc, argv); } catch (args::Help) { std::cerr << parser; parsing_result = HELP; return; } catch (args::ParseError e) { std::cerr << e.what() << "\n"; parsing_result = BAD; return; } catch (args::ValidationError e) { std::cerr << e.what() << "\n"; parsing_result = BAD; return; } if (argc == 1) { std::cerr << parser; parsing_result = HELP; return; } if (args::get(version_arg)) { parsing_result = VERSION; return; } input_reads = args::get(input_reads_arg); if (input_reads.empty()) { std::cerr << "Error: input reads are required" << "\n"; parsing_result = BAD; return; } target_bases_set = bool(target_bases_arg); target_bases = args::get(target_bases_arg); keep_percent_set = bool(keep_percent_arg); keep_percent = args::get(keep_percent_arg); assembly_set = bool(assembly_arg); assembly = args::get(assembly_arg); if (bool(illumina_1_arg)) illumina_reads.push_back(args::get(illumina_1_arg)); if (bool(illumina_2_arg)) illumina_reads.push_back(args::get(illumina_2_arg)); min_length_set = bool(min_length_arg); min_length = args::get(min_length_arg); max_length_set = bool(max_length_arg); max_length = args::get(max_length_arg); min_mean_q_set = bool(min_mean_q_arg); min_mean_q = args::get(min_mean_q_arg); min_window_q_set = bool(min_window_q_arg); min_window_q = args::get(min_window_q_arg); length_weight = args::get(length_weight_arg); mean_q_weight = args::get(mean_q_weight_arg); window_q_weight = args::get(window_q_weight_arg); trim = args::get(trim_arg); split_set = bool(split_arg); split = args::get(split_arg); window_size = args::get(window_size_arg); verbose = args::get(verbose_arg); bool some_reference = (illumina_reads.size() > 0 || assembly_set); if (trim && !some_reference) { std::cerr << "Error: assembly or read reference is required to use --trim" << "\n"; parsing_result = BAD; return; } if (split_set && !some_reference) { std::cerr << "Error: assembly or read reference is required to use --split" << "\n"; parsing_result = BAD; return; } // Check to make sure files exist. std::vector files; files.push_back(input_reads); for (auto f : illumina_reads) files.push_back(f); if (assembly_set) files.push_back(assembly); for (auto f : files) { if (!does_file_exist(f)) { std::cerr << "Error: cannot find file: " << f << "\n"; parsing_result = BAD; return; } } // If nothing is set, then Filtlong won't do anything. Give an error message and quit. if (!trim && !split_set && !target_bases_set && !keep_percent_set && !min_length_set && !max_length_set && !min_mean_q_set && !min_window_q_set) { std::cerr << "Error: no thresholds set, you must use one of the following options:\n"; std::cerr << "target_bases, keep_percent, min_length, max_length, min_mean_q, min_window_q, trim, split\n"; parsing_result = BAD; return; } // Non-positive target_bases doesn't make sense. if (target_bases_set && target_bases <= 0) { std::cerr << "Error: the value for --target_bases must be a positive integer\n"; parsing_result = BAD; return; } // Non-positive min_length doesn't make sense. if (min_length_set && min_length <= 0) { std::cerr << "Error: the value for --min_length must be a positive integer\n"; parsing_result = BAD; return; } // Non-positive max_length doesn't make sense. if (max_length_set && max_length <= 0) { std::cerr << "Error: the value for --max_length must be a positive integer\n"; parsing_result = BAD; return; } // keep_percent must be between 0 and 100 (exclusive). if (keep_percent_set && (keep_percent <= 0.0 || keep_percent >= 100.0)) { std::cerr << "Error: the value for --keep_percent must be greater than 0 and less than 100\n"; parsing_result = BAD; return; } // min_mean_q and min_window_q must be positive. if (min_mean_q_set && min_mean_q <= 0.0) { std::cerr << "Error: the value for --min_mean_q must be greater than 0\n"; parsing_result = BAD; return; } if (min_window_q_set && min_window_q <= 0.0) { std::cerr << "Error: the value for --min_window_q must be greater than 0\n"; parsing_result = BAD; return; } // Negative weights don't make sense. if (length_weight < 0.0 || mean_q_weight < 0.0 || window_q_weight < 0.0) { std::cerr << "Error: weight values cannot be negative\n"; parsing_result = BAD; return; } // Non-positive split doesn't make sense. if (split_set && split <= 0) { std::cerr << "Error: the value for --split must be a positive integer\n"; parsing_result = BAD; return; } // Non-positive window_size doesn't make sense. if (window_size <= 0) { std::cerr << "Error: the value for --window_size must be a positive integer\n"; parsing_result = BAD; return; } } bool Arguments::does_file_exist(std::string filename){ std::ifstream infile(filename); return infile.good(); } Filtlong-0.2.1/src/arguments.h000066400000000000000000000031021406722713100162340ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #ifndef ARGUMENTS_H #define ARGUMENTS_H #include #include enum ParsingResult {GOOD, BAD, HELP, VERSION}; class Arguments { public: Arguments(int argc, char **argv); ParsingResult parsing_result; std::string input_reads; bool target_bases_set; long long target_bases; bool keep_percent_set; double keep_percent; bool min_length_set; int min_length; bool max_length_set; int max_length; bool min_mean_q_set; double min_mean_q; bool min_window_q_set; double min_window_q; bool assembly_set; std::string assembly; std::vector illumina_reads; double length_weight; double mean_q_weight; double window_q_weight; bool trim; bool split_set; int split; int window_size; bool verbose; private: bool does_file_exist(std::string fileName); }; #endif // ARGUMENTS_H Filtlong-0.2.1/src/bloom_filter.h000066400000000000000000000540761406722713100167240ustar00rootroot00000000000000/* ********************************************************************* * * * Open Bloom Filter * * * * Author: Arash Partow - 2000 * * URL: http://www.partow.net * * URL: http://www.partow.net/programming/hashfunctions/index.html * * * * Copyright notice: * * Free use of the Open Bloom Filter Library is permitted under the * * guidelines and in accordance with the MIT License. * * http://www.opensource.org/licenses/MIT * * * ********************************************************************* */ #ifndef INCLUDE_BLOOM_FILTER_HPP #define INCLUDE_BLOOM_FILTER_HPP #include #include #include #include #include #include #include #include static const std::size_t bits_per_char = 0x08; // 8 bits in 1 char(unsigned) static const unsigned char bit_mask[bits_per_char] = { 0x01, //00000001 0x02, //00000010 0x04, //00000100 0x08, //00001000 0x10, //00010000 0x20, //00100000 0x40, //01000000 0x80 //10000000 }; class bloom_parameters { public: bloom_parameters() : minimum_size(1), maximum_size(std::numeric_limits::max()), minimum_number_of_hashes(1), maximum_number_of_hashes(std::numeric_limits::max()), projected_element_count(10000), false_positive_probability(1.0 / projected_element_count), random_seed(0xA5A5A5A55A5A5A5AULL) {} virtual ~bloom_parameters() {} inline bool operator!() { return (minimum_size > maximum_size) || (minimum_number_of_hashes > maximum_number_of_hashes) || (minimum_number_of_hashes < 1) || (0 == maximum_number_of_hashes) || (0 == projected_element_count) || (false_positive_probability < 0.0) || (std::numeric_limits::infinity() == std::abs(false_positive_probability)) || (0 == random_seed) || (0xFFFFFFFFFFFFFFFFULL == random_seed); } // Allowable min/max size of the bloom filter in bits unsigned long long int minimum_size; unsigned long long int maximum_size; // Allowable min/max number of hash functions unsigned int minimum_number_of_hashes; unsigned int maximum_number_of_hashes; // The approximate number of elements to be inserted // into the bloom filter, should be within one order // of magnitude. The default is 10000. unsigned long long int projected_element_count; // The approximate false positive probability expected // from the bloom filter. The default is assumed to be // the reciprocal of the projected_element_count. double false_positive_probability; unsigned long long int random_seed; struct optimal_parameters_t { optimal_parameters_t() : number_of_hashes(0), table_size(0) {} unsigned int number_of_hashes; unsigned long long int table_size; }; optimal_parameters_t optimal_parameters; virtual bool compute_optimal_parameters() { /* Note: The following will attempt to find the number of hash functions and minimum amount of storage bits required to construct a bloom filter consistent with the user defined false positive probability and estimated element insertion count. */ if (!(*this)) return false; double min_m = std::numeric_limits::infinity(); double min_k = 0.0; double k = 1.0; while (k < 1000.0) { const double numerator = (- k * projected_element_count); const double denominator = std::log(1.0 - std::pow(false_positive_probability, 1.0 / k)); const double curr_m = numerator / denominator; if (curr_m < min_m) { min_m = curr_m; min_k = k; } k += 1.0; } optimal_parameters_t& optp = optimal_parameters; optp.number_of_hashes = static_cast(min_k); optp.table_size = static_cast(min_m); optp.table_size += (((optp.table_size % bits_per_char) != 0) ? (bits_per_char - (optp.table_size % bits_per_char)) : 0); if (optp.number_of_hashes < minimum_number_of_hashes) optp.number_of_hashes = minimum_number_of_hashes; else if (optp.number_of_hashes > maximum_number_of_hashes) optp.number_of_hashes = maximum_number_of_hashes; if (optp.table_size < minimum_size) optp.table_size = minimum_size; else if (optp.table_size > maximum_size) optp.table_size = maximum_size; return true; } }; class bloom_filter { protected: typedef unsigned int bloom_type; typedef unsigned char cell_type; typedef std::vector table_type; public: bloom_filter() : salt_count_(0), table_size_(0), projected_element_count_(0), inserted_element_count_ (0), random_seed_(0), desired_false_positive_probability_(0.0) {} bloom_filter(const bloom_parameters& p) : projected_element_count_(p.projected_element_count), inserted_element_count_(0), random_seed_((p.random_seed * 0xA5A5A5A5) + 1), desired_false_positive_probability_(p.false_positive_probability) { salt_count_ = p.optimal_parameters.number_of_hashes; table_size_ = p.optimal_parameters.table_size; generate_unique_salt(); bit_table_.resize(table_size_ / bits_per_char, static_cast(0x00)); } bloom_filter(const bloom_filter& filter) { this->operator=(filter); } inline bool operator == (const bloom_filter& f) const { if (this != &f) { return (salt_count_ == f.salt_count_ ) && (table_size_ == f.table_size_ ) && (bit_table_.size() == f.bit_table_.size() ) && (projected_element_count_ == f.projected_element_count_ ) && (inserted_element_count_ == f.inserted_element_count_ ) && (random_seed_ == f.random_seed_ ) && (desired_false_positive_probability_ == f.desired_false_positive_probability_) && (salt_ == f.salt_ ) && (bit_table_ == f.bit_table_ ) ; } else return true; } inline bool operator != (const bloom_filter& f) const { return !operator==(f); } inline bloom_filter& operator = (const bloom_filter& f) { if (this != &f) { salt_count_ = f.salt_count_; table_size_ = f.table_size_; bit_table_ = f.bit_table_; salt_ = f.salt_; projected_element_count_ = f.projected_element_count_; inserted_element_count_ = f.inserted_element_count_; random_seed_ = f.random_seed_; desired_false_positive_probability_ = f.desired_false_positive_probability_; } return *this; } virtual ~bloom_filter() {} inline bool operator!() const { return (0 == table_size_); } inline void clear() { std::fill(bit_table_.begin(), bit_table_.end(), static_cast(0x00)); inserted_element_count_ = 0; } inline void insert(const unsigned char* key_begin, const std::size_t& length) { std::size_t bit_index = 0; std::size_t bit = 0; for (std::size_t i = 0; i < salt_.size(); ++i) { compute_indices(hash_ap(key_begin, length, salt_[i]), bit_index, bit); bit_table_[bit_index / bits_per_char] |= bit_mask[bit]; } ++inserted_element_count_; } template inline void insert(const T& t) { // Note: T must be a C++ POD type. insert(reinterpret_cast(&t),sizeof(T)); } inline void insert(const std::string& key) { insert(reinterpret_cast(key.data()),key.size()); } inline void insert(const char* data, const std::size_t& length) { insert(reinterpret_cast(data),length); } template inline void insert(const InputIterator begin, const InputIterator end) { InputIterator itr = begin; while (end != itr) { insert(*(itr++)); } } inline virtual bool contains(const unsigned char* key_begin, const std::size_t length) const { std::size_t bit_index = 0; std::size_t bit = 0; for (std::size_t i = 0; i < salt_.size(); ++i) { compute_indices(hash_ap(key_begin, length, salt_[i]), bit_index, bit); if ((bit_table_[bit_index / bits_per_char] & bit_mask[bit]) != bit_mask[bit]) { return false; } } return true; } template inline bool contains(const T& t) const { return contains(reinterpret_cast(&t),static_cast(sizeof(T))); } inline bool contains(const std::string& key) const { return contains(reinterpret_cast(key.c_str()),key.size()); } inline bool contains(const char* data, const std::size_t& length) const { return contains(reinterpret_cast(data),length); } template inline InputIterator contains_all(const InputIterator begin, const InputIterator end) const { InputIterator itr = begin; while (end != itr) { if (!contains(*itr)) { return itr; } ++itr; } return end; } template inline InputIterator contains_none(const InputIterator begin, const InputIterator end) const { InputIterator itr = begin; while (end != itr) { if (contains(*itr)) { return itr; } ++itr; } return end; } inline virtual unsigned long long int size() const { return table_size_; } inline unsigned long long int element_count() const { return inserted_element_count_; } inline double effective_fpp() const { /* Note: The effective false positive probability is calculated using the designated table size and hash function count in conjunction with the current number of inserted elements - not the user defined predicated/expected number of inserted elements. */ return std::pow(1.0 - std::exp(-1.0 * salt_.size() * inserted_element_count_ / size()), 1.0 * salt_.size()); } inline bloom_filter& operator &= (const bloom_filter& f) { /* intersection */ if ( (salt_count_ == f.salt_count_ ) && (table_size_ == f.table_size_ ) && (random_seed_ == f.random_seed_) ) { for (std::size_t i = 0; i < bit_table_.size(); ++i) { bit_table_[i] &= f.bit_table_[i]; } } return *this; } inline bloom_filter& operator |= (const bloom_filter& f) { /* union */ if ( (salt_count_ == f.salt_count_ ) && (table_size_ == f.table_size_ ) && (random_seed_ == f.random_seed_) ) { for (std::size_t i = 0; i < bit_table_.size(); ++i) { bit_table_[i] |= f.bit_table_[i]; } } return *this; } inline bloom_filter& operator ^= (const bloom_filter& f) { /* difference */ if ( (salt_count_ == f.salt_count_ ) && (table_size_ == f.table_size_ ) && (random_seed_ == f.random_seed_) ) { for (std::size_t i = 0; i < bit_table_.size(); ++i) { bit_table_[i] ^= f.bit_table_[i]; } } return *this; } inline const cell_type* table() const { return bit_table_.data(); } inline std::size_t hash_count() { return salt_.size(); } protected: inline virtual void compute_indices(const bloom_type& hash, std::size_t& bit_index, std::size_t& bit) const { bit_index = hash % table_size_; bit = bit_index % bits_per_char; } void generate_unique_salt() { /* Note: A distinct hash function need not be implementation-wise distinct. In the current implementation "seeding" a common hash function with different values seems to be adequate. */ const unsigned int predef_salt_count = 128; static const bloom_type predef_salt[predef_salt_count] = { 0xAAAAAAAA, 0x55555555, 0x33333333, 0xCCCCCCCC, 0x66666666, 0x99999999, 0xB5B5B5B5, 0x4B4B4B4B, 0xAA55AA55, 0x55335533, 0x33CC33CC, 0xCC66CC66, 0x66996699, 0x99B599B5, 0xB54BB54B, 0x4BAA4BAA, 0xAA33AA33, 0x55CC55CC, 0x33663366, 0xCC99CC99, 0x66B566B5, 0x994B994B, 0xB5AAB5AA, 0xAAAAAA33, 0x555555CC, 0x33333366, 0xCCCCCC99, 0x666666B5, 0x9999994B, 0xB5B5B5AA, 0xFFFFFFFF, 0xFFFF0000, 0xB823D5EB, 0xC1191CDF, 0xF623AEB3, 0xDB58499F, 0xC8D42E70, 0xB173F616, 0xA91A5967, 0xDA427D63, 0xB1E8A2EA, 0xF6C0D155, 0x4909FEA3, 0xA68CC6A7, 0xC395E782, 0xA26057EB, 0x0CD5DA28, 0x467C5492, 0xF15E6982, 0x61C6FAD3, 0x9615E352, 0x6E9E355A, 0x689B563E, 0x0C9831A8, 0x6753C18B, 0xA622689B, 0x8CA63C47, 0x42CC2884, 0x8E89919B, 0x6EDBD7D3, 0x15B6796C, 0x1D6FDFE4, 0x63FF9092, 0xE7401432, 0xEFFE9412, 0xAEAEDF79, 0x9F245A31, 0x83C136FC, 0xC3DA4A8C, 0xA5112C8C, 0x5271F491, 0x9A948DAB, 0xCEE59A8D, 0xB5F525AB, 0x59D13217, 0x24E7C331, 0x697C2103, 0x84B0A460, 0x86156DA9, 0xAEF2AC68, 0x23243DA5, 0x3F649643, 0x5FA495A8, 0x67710DF8, 0x9A6C499E, 0xDCFB0227, 0x46A43433, 0x1832B07A, 0xC46AFF3C, 0xB9C8FFF0, 0xC9500467, 0x34431BDF, 0xB652432B, 0xE367F12B, 0x427F4C1B, 0x224C006E, 0x2E7E5A89, 0x96F99AA5, 0x0BEB452A, 0x2FD87C39, 0x74B2E1FB, 0x222EFD24, 0xF357F60C, 0x440FCB1E, 0x8BBE030F, 0x6704DC29, 0x1144D12F, 0x948B1355, 0x6D8FD7E9, 0x1C11A014, 0xADD1592F, 0xFB3C712E, 0xFC77642F, 0xF9C4CE8C, 0x31312FB9, 0x08B0DD79, 0x318FA6E7, 0xC040D23D, 0xC0589AA7, 0x0CA5C075, 0xF874B172, 0x0CF914D5, 0x784D3280, 0x4E8CFEBC, 0xC569F575, 0xCDB2A091, 0x2CC016B4, 0x5C5F4421 }; if (salt_count_ <= predef_salt_count) { std::copy(predef_salt, predef_salt + salt_count_, std::back_inserter(salt_)); for (std::size_t i = 0; i < salt_.size(); ++i) { /* Note: This is done to integrate the user defined random seed, so as to allow for the generation of unique bloom filter instances. */ salt_[i] = salt_[i] * salt_[(i + 3) % salt_.size()] + static_cast(random_seed_); } } else { std::copy(predef_salt, predef_salt + predef_salt_count, std::back_inserter(salt_)); srand(static_cast(random_seed_)); while (salt_.size() < salt_count_) { bloom_type current_salt = static_cast(rand()) * static_cast(rand()); if (0 == current_salt) continue; if (salt_.end() == std::find(salt_.begin(), salt_.end(), current_salt)) { salt_.push_back(current_salt); } } } } inline bloom_type hash_ap(const unsigned char* begin, std::size_t remaining_length, bloom_type hash) const { const unsigned char* itr = begin; unsigned int loop = 0; while (remaining_length >= 8) { const unsigned int& i1 = *(reinterpret_cast(itr)); itr += sizeof(unsigned int); const unsigned int& i2 = *(reinterpret_cast(itr)); itr += sizeof(unsigned int); hash ^= (hash << 7) ^ i1 * (hash >> 3) ^ (~((hash << 11) + (i2 ^ (hash >> 5)))); remaining_length -= 8; } if (remaining_length) { if (remaining_length >= 4) { const unsigned int& i = *(reinterpret_cast(itr)); if (loop & 0x01) hash ^= (hash << 7) ^ i * (hash >> 3); else hash ^= (~((hash << 11) + (i ^ (hash >> 5)))); ++loop; remaining_length -= 4; itr += sizeof(unsigned int); } if (remaining_length >= 2) { const unsigned short& i = *(reinterpret_cast(itr)); if (loop & 0x01) hash ^= (hash << 7) ^ i * (hash >> 3); else hash ^= (~((hash << 11) + (i ^ (hash >> 5)))); ++loop; remaining_length -= 2; itr += sizeof(unsigned short); } if (remaining_length) { hash += ((*itr) ^ (hash * 0xA5A5A5A5)) + loop; } } return hash; } std::vector salt_; std::vector bit_table_; unsigned int salt_count_; unsigned long long int table_size_; unsigned long long int projected_element_count_; unsigned long long int inserted_element_count_; unsigned long long int random_seed_; double desired_false_positive_probability_; }; inline bloom_filter operator & (const bloom_filter& a, const bloom_filter& b) { bloom_filter result = a; result &= b; return result; } inline bloom_filter operator | (const bloom_filter& a, const bloom_filter& b) { bloom_filter result = a; result |= b; return result; } inline bloom_filter operator ^ (const bloom_filter& a, const bloom_filter& b) { bloom_filter result = a; result ^= b; return result; } class compressible_bloom_filter : public bloom_filter { public: compressible_bloom_filter(const bloom_parameters& p) : bloom_filter(p) { size_list.push_back(table_size_); } inline unsigned long long int size() const { return size_list.back(); } inline bool compress(const double& percentage) { if ( (percentage < 0.0) || (percentage >= 100.0) ) { return false; } unsigned long long int original_table_size = size_list.back(); unsigned long long int new_table_size = static_cast((size_list.back() * (1.0 - (percentage / 100.0)))); new_table_size -= new_table_size % bits_per_char; if ( (bits_per_char > new_table_size) || (new_table_size >= original_table_size) ) { return false; } desired_false_positive_probability_ = effective_fpp(); const unsigned long long int new_tbl_raw_size = new_table_size / bits_per_char; table_type tmp(new_tbl_raw_size); std::copy(bit_table_.begin(), bit_table_.begin() + new_tbl_raw_size, tmp.begin()); typedef table_type::iterator itr_t; itr_t itr = bit_table_.begin() + (new_table_size / bits_per_char); itr_t end = bit_table_.begin() + (original_table_size / bits_per_char); itr_t itr_tmp = tmp.begin(); while (end != itr) { *(itr_tmp++) |= (*itr++); } std::swap(bit_table_, tmp); size_list.push_back(new_table_size); return true; } private: inline void compute_indices(const bloom_type& hash, std::size_t& bit_index, std::size_t& bit) const { bit_index = hash; for (std::size_t i = 0; i < size_list.size(); ++i) { bit_index %= size_list[i]; } bit = bit_index % bits_per_char; } std::vector size_list; }; #endif /* Note 1: If it can be guaranteed that bits_per_char will be of the form 2^n then the following optimization can be used: bit_table_[bit_index >> n] |= bit_mask[bit_index & (bits_per_char - 1)]; Note 2: For performance reasons where possible when allocating memory it should be aligned (aligned_alloc) according to the architecture being used. */ Filtlong-0.2.1/src/kmers.cpp000066400000000000000000000160031406722713100157070ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #include "kmers.h" #include #include #include #include "kseq.h" #include "misc.h" KSEQ_INIT(gzFile, gzread) Kmers::Kmers() { bloom_parameters parameters; // TO DO: it might be worth experimenting with these values to see how it affects time and memory usage. parameters.projected_element_count = 100000000; parameters.false_positive_probability = 0.0001; // 1 in 10000 parameters.random_seed = 0xA5A5A5A5; parameters.compute_optimal_parameters(); //Instantiate Bloom Filter bloom = new bloom_filter(parameters); required_kmer_copies = 4; } Kmers::~Kmers() { delete bloom; } void Kmers::add_read_fastqs(std::vector filenames) { std::cerr << "Hashing 16-mers from Illumina reads\n"; int sequence_count = 0; for (auto & filename : filenames) sequence_count += add_reference(filename, true); std::cerr << " " << int_to_string(sequence_count) << " reads, " << int_to_string(m_kmers.size()) << " 16-mers\n\n"; } void Kmers::add_assembly_fasta(std::string filename) { std::cerr << "Hashing 16-mers from assembly\n"; std::cerr << " " << filename << "\n"; int sequence_count = add_reference(filename, false); std::string noun; if (sequence_count == 1) noun = "contig"; else noun = "contigs"; std::cerr << " " << int_to_string(sequence_count) << " " << noun << ", " << int_to_string(m_kmers.size()) << " 16-mers\n\n"; } int Kmers::add_reference(std::string filename, bool require_two_kmer_copies) { int l; uint32_t forward_kmer, reverse_kmer; int sequence_count = 0; // We'll use a different k-mer adding function for assembly hashing and read hashing. void (Kmers::*add_kmer)(uint32_t); if (require_two_kmer_copies) add_kmer = &Kmers::add_kmer_require_multiple_copies; else add_kmer = &Kmers::add_kmer_require_one_copy; long long base_count = 0; long long last_progress = 0; gzFile fp = gzopen(filename.c_str(), "r"); kseq_t * seq = kseq_init(fp); while ((l = kseq_read(seq)) >= 0) { if (l == -3) std::cerr << "Error reading " << filename << "\n"; else { ++sequence_count; // Can't get a 16-mer from a sequence shorter than 16 bp. if (seq->seq.l < 16) continue; base_count += seq->seq.l; char * sequence = seq->seq.s; // Build the starting k-mers from the first 16 bases. forward_kmer = starting_kmer_to_bits_forward(sequence); reverse_kmer = starting_kmer_to_bits_reverse(sequence); (this->*add_kmer)(forward_kmer); (this->*add_kmer)(reverse_kmer); for (size_t i = 16; i < seq->seq.l; ++i) { forward_kmer <<= 2; forward_kmer |= base_to_bits_forward(sequence[i]); reverse_kmer >>= 2; reverse_kmer |= base_to_bits_reverse(sequence[i]); (this->*add_kmer)(forward_kmer); (this->*add_kmer)(reverse_kmer); } if (base_count - last_progress >= 483611) { // a big prime number so progress updates don't round off last_progress = base_count; print_hash_progress(filename, base_count); } } } kseq_destroy(seq); gzclose(fp); print_hash_progress(filename, base_count); std::cerr << "\n"; return sequence_count; } void Kmers::add_kmer_require_one_copy(uint32_t kmer) { m_kmers.insert(kmer); } void Kmers::add_kmer_require_multiple_copies(uint32_t kmer) { // If the kmer is already in the final set, then we can skip the rest of this function. if (m_kmers.find(kmer) != m_kmers.end()) return; // Check the bloom filter. If it's not in there, this is definitely the first time it's been seen. if (!bloom->contains(kmer)) bloom->insert(kmer); // If it's in the bloom filter, then it's probably been seen once before (though maybe not, based on the false // positive rate of the bloom filter. Next we check the k-mer counts. If it's not in there, we say it's the second // time the kmer's been seen. else if (m_kmer_counts.find(kmer) == m_kmer_counts.end()) m_kmer_counts[kmer] = 2; // If the k-mer is in the counts, then we increment its count. If the count is high enough, we add it to the k-mer // set (and remove it from the counts to save some memory). else { int times_seen = ++m_kmer_counts[kmer]; if (times_seen >= required_kmer_copies) { m_kmers.insert(kmer); m_kmer_counts.erase(kmer); } } } bool Kmers::is_kmer_present(uint32_t kmer) { return m_kmers.find(kmer) != m_kmers.end(); } uint32_t Kmers::base_to_bits_forward(char base) { switch (base) { case 'A': return 0; // 00000000000000000000000000000000 case 'C': return 1; // 00000000000000000000000000000001 case 'G': return 2; // 00000000000000000000000000000010 case 'T': return 3; // 00000000000000000000000000000011 case 'a': return 0; case 'c': return 1; case 'g': return 2; case 't': return 3; } return 0; } uint32_t Kmers::base_to_bits_reverse(char base) { switch (base) { case 'T': return 0; // 00000000000000000000000000000000 case 'G': return 1073741824; // 01000000000000000000000000000000 case 'C': return 2147483648; // 10000000000000000000000000000000 case 'A': return 3221225472; // 11000000000000000000000000000000 case 't': return 0; case 'g': return 1073741824; case 'c': return 2147483648; case 'a': return 3221225472; } return 0; } uint32_t Kmers::starting_kmer_to_bits_forward(char * sequence) { uint32_t kmer = 0; for (int i = 0; i < 16; ++i) { kmer <<= 2; kmer |= base_to_bits_forward(sequence[i]); } return kmer; } uint32_t Kmers::starting_kmer_to_bits_reverse(char * sequence) { uint32_t kmer = 0; for (int i = 0; i < 16; ++i) { kmer >>= 2; kmer |= base_to_bits_reverse(sequence[i]); } return kmer; } Filtlong-0.2.1/src/kmers.h000066400000000000000000000032111406722713100153510ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #ifndef KMERS_H #define KMERS_H #include #include #include #include #include "bloom_filter.h" class Kmers { public: Kmers(); ~Kmers(); bool empty() {return m_kmers.size() == 0;} void add_read_fastqs(std::vector filenames); void add_assembly_fasta(std::string filename); bool is_kmer_present(uint32_t kmer); uint32_t starting_kmer_to_bits_forward(char * sequence); uint32_t starting_kmer_to_bits_reverse(char * sequence); uint32_t base_to_bits_forward(char base); uint32_t base_to_bits_reverse(char base); private: std::unordered_set m_kmers; std::unordered_map m_kmer_counts; bloom_filter * bloom; int required_kmer_copies; int add_reference(std::string filename, bool require_two_kmer_copies); void add_kmer_require_one_copy(uint32_t kmer); void add_kmer_require_multiple_copies(uint32_t kmer); }; #endif // KMERS_H Filtlong-0.2.1/src/kseq.h000066400000000000000000000216441406722713100152050ustar00rootroot00000000000000/* The MIT License Copyright (c) 2008, 2009, 2011 Attractive Chaos Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ /* Last Modified: 05MAR2012 */ #ifndef AC_KSEQ_H #define AC_KSEQ_H #include #include #include #define KS_SEP_SPACE 0 // isspace(): \t, \n, \v, \f, \r #define KS_SEP_TAB 1 // isspace() && !' ' #define KS_SEP_LINE 2 // line separator: "\n" (Unix) or "\r\n" (Windows) #define KS_SEP_MAX 2 #define __KS_TYPE(type_t) \ typedef struct __kstream_t { \ unsigned char *buf; \ int begin, end, is_eof; \ type_t f; \ } kstream_t; #define ks_err(ks) ((ks)->end == -1) #define ks_eof(ks) ((ks)->is_eof && (ks)->begin >= (ks)->end) #define ks_rewind(ks) ((ks)->is_eof = (ks)->begin = (ks)->end = 0) #define __KS_BASIC(type_t, __bufsize) \ static inline kstream_t *ks_init(type_t f) \ { \ kstream_t *ks = (kstream_t*)calloc(1, sizeof(kstream_t)); \ ks->f = f; \ ks->buf = (unsigned char*)malloc(__bufsize); \ return ks; \ } \ static inline void ks_destroy(kstream_t *ks) \ { \ if (ks) { \ free(ks->buf); \ free(ks); \ } \ } #define __KS_GETC(__read, __bufsize) \ static inline int ks_getc(kstream_t *ks) \ { \ if (ks_err(ks)) return -3; \ if (ks->is_eof && ks->begin >= ks->end) return -1; \ if (ks->begin >= ks->end) { \ ks->begin = 0; \ ks->end = __read(ks->f, ks->buf, __bufsize); \ if (ks->end == 0) { ks->is_eof = 1; return -1;} \ if (ks->end == -1) { ks->is_eof = 1; return -3;}\ } \ return (int)ks->buf[ks->begin++]; \ } #ifndef KSTRING_T #define KSTRING_T kstring_t typedef struct __kstring_t { size_t l, m; char *s; } kstring_t; #endif #ifndef kroundup32 #define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x)) #endif #define __KS_GETUNTIL(__read, __bufsize) \ static int ks_getuntil2(kstream_t *ks, int delimiter, kstring_t *str, int *dret, int append) \ { \ int gotany = 0; \ if (dret) *dret = 0; \ str->l = append? str->l : 0; \ for (;;) { \ int i; \ if (ks_err(ks)) return -3; \ if (ks->begin >= ks->end) { \ if (!ks->is_eof) { \ ks->begin = 0; \ ks->end = __read(ks->f, ks->buf, __bufsize); \ if (ks->end == 0) { ks->is_eof = 1; break; } \ if (ks->end == -1) { ks->is_eof = 1; return -3; } \ } else break; \ } \ if (delimiter == KS_SEP_LINE) { \ for (i = ks->begin; i < ks->end; ++i) \ if (ks->buf[i] == '\n') break; \ } else if (delimiter > KS_SEP_MAX) { \ for (i = ks->begin; i < ks->end; ++i) \ if (ks->buf[i] == delimiter) break; \ } else if (delimiter == KS_SEP_SPACE) { \ for (i = ks->begin; i < ks->end; ++i) \ if (isspace(ks->buf[i])) break; \ } else if (delimiter == KS_SEP_TAB) { \ for (i = ks->begin; i < ks->end; ++i) \ if (isspace(ks->buf[i]) && ks->buf[i] != ' ') break; \ } else i = 0; /* never come to here! */ \ if (str->m - str->l < (size_t)(i - ks->begin + 1)) { \ str->m = str->l + (i - ks->begin) + 1; \ kroundup32(str->m); \ str->s = (char*)realloc(str->s, str->m); \ } \ gotany = 1; \ memcpy(str->s + str->l, ks->buf + ks->begin, i - ks->begin); \ str->l = str->l + (i - ks->begin); \ ks->begin = i + 1; \ if (i < ks->end) { \ if (dret) *dret = ks->buf[i]; \ break; \ } \ } \ if (!gotany && ks_eof(ks)) return -1; \ if (str->s == 0) { \ str->m = 1; \ str->s = (char*)calloc(1, 1); \ } else if (delimiter == KS_SEP_LINE && str->l > 1 && str->s[str->l-1] == '\r') --str->l; \ str->s[str->l] = '\0'; \ return str->l; \ } \ static inline int ks_getuntil(kstream_t *ks, int delimiter, kstring_t *str, int *dret) \ { return ks_getuntil2(ks, delimiter, str, dret, 0); } #define KSTREAM_INIT(type_t, __read, __bufsize) \ __KS_TYPE(type_t) \ __KS_BASIC(type_t, __bufsize) \ __KS_GETC(__read, __bufsize) \ __KS_GETUNTIL(__read, __bufsize) #define kseq_rewind(ks) ((ks)->last_char = (ks)->f->is_eof = (ks)->f->begin = (ks)->f->end = 0) #define __KSEQ_BASIC(SCOPE, type_t) \ SCOPE kseq_t *kseq_init(type_t fd) \ { \ kseq_t *s = (kseq_t*)calloc(1, sizeof(kseq_t)); \ s->f = ks_init(fd); \ return s; \ } \ SCOPE void kseq_destroy(kseq_t *ks) \ { \ if (!ks) return; \ free(ks->name.s); free(ks->comment.s); free(ks->seq.s); free(ks->qual.s); \ ks_destroy(ks->f); \ free(ks); \ } /* Return value: >=0 length of the sequence (normal) -1 end-of-file -2 truncated quality string -3 error reading stream */ #define __KSEQ_READ(SCOPE) \ SCOPE int kseq_read(kseq_t *seq) \ { \ int c,r; \ kstream_t *ks = seq->f; \ if (seq->last_char == 0) { /* then jump to the next header line */ \ while ((c = ks_getc(ks)) >= 0 && c != '>' && c != '@'); \ if (c < 0) return c; /* end of file or error*/ \ seq->last_char = c; \ } /* else: the first header char has been read in the previous call */ \ seq->comment.l = seq->seq.l = seq->qual.l = 0; /* reset all members */ \ if ((r=ks_getuntil(ks, 0, &seq->name, &c)) < 0) return r; /* normal exit: EOF or error */ \ if (c != '\n') ks_getuntil(ks, KS_SEP_LINE, &seq->comment, 0); /* read FASTA/Q comment */ \ if (seq->seq.s == 0) { /* we can do this in the loop below, but that is slower */ \ seq->seq.m = 256; \ seq->seq.s = (char*)malloc(seq->seq.m); \ } \ while ((c = ks_getc(ks)) >= 0 && c != '>' && c != '+' && c != '@') { \ if (c == '\n') continue; /* skip empty lines */ \ seq->seq.s[seq->seq.l++] = c; /* this is safe: we always have enough space for 1 char */ \ ks_getuntil2(ks, KS_SEP_LINE, &seq->seq, 0, 1); /* read the rest of the line */ \ } \ if (c == '>' || c == '@') seq->last_char = c; /* the first header char has been read */ \ if (seq->seq.l + 1 >= seq->seq.m) { /* seq->seq.s[seq->seq.l] below may be out of boundary */ \ seq->seq.m = seq->seq.l + 2; \ kroundup32(seq->seq.m); /* rounded to the next closest 2^k */ \ seq->seq.s = (char*)realloc(seq->seq.s, seq->seq.m); \ } \ seq->seq.s[seq->seq.l] = 0; /* null terminated string */ \ if (c != '+') return seq->seq.l; /* FASTA */ \ if (seq->qual.m < seq->seq.m) { /* allocate memory for qual in case insufficient */ \ seq->qual.m = seq->seq.m; \ seq->qual.s = (char*)realloc(seq->qual.s, seq->qual.m); \ } \ while ((c = ks_getc(ks)) >= 0 && c != '\n'); /* skip the rest of '+' line */ \ if (c == -1) return -2; /* error: no quality string */ \ while ((c = ks_getuntil2(ks, KS_SEP_LINE, &seq->qual, 0, 1) >= 0 && seq->qual.l < seq->seq.l)); \ if (c == -3) return -3; /* stream error */ \ seq->last_char = 0; /* we have not come to the next header line */ \ if (seq->seq.l != seq->qual.l) return -2; /* error: qual string is of a different length */ \ return seq->seq.l; \ } #define __KSEQ_TYPE(type_t) \ typedef struct { \ kstring_t name, comment, seq, qual; \ int last_char; \ kstream_t *f; \ } kseq_t; #define KSEQ_INIT2(SCOPE, type_t, __read) \ KSTREAM_INIT(type_t, __read, 16384) \ __KSEQ_TYPE(type_t) \ __KSEQ_BASIC(SCOPE, type_t) \ __KSEQ_READ(SCOPE) #define KSEQ_INIT(type_t, __read) KSEQ_INIT2(static, type_t, __read) #define KSEQ_DECLARE(type_t) \ __KS_TYPE(type_t) \ __KSEQ_TYPE(type_t) \ extern kseq_t *kseq_init(type_t fd); \ void kseq_destroy(kseq_t *ks); \ int kseq_read(kseq_t *seq); #endif Filtlong-0.2.1/src/main.cpp000066400000000000000000000273261406722713100155240ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #include #include #include #include #include #include #include #include #include "kseq.h" #include "read.h" #include "arguments.h" #include "kmers.h" #include "misc.h" #define PROGRAM_VERSION "0.2.1" KSEQ_INIT(gzFile, gzread) int main(int argc, char **argv) { Arguments args(argc, argv); if (args.parsing_result == BAD) return 1; else if (args.parsing_result == HELP) return 0; else if (args.parsing_result == VERSION) { std::cout << "Filtlong v" << PROGRAM_VERSION << "\n"; return 0; } std::cerr << "\n"; // Read through references and save 16-mers. For assembly references, this will save all 16-mers in the assembly. // For Illumina read references, the k-mer needs to appear a few times before it's added to the set. Kmers kmers; if (args.assembly_set || args.illumina_reads.size() > 0) { if (args.assembly_set) kmers.add_assembly_fasta(args.assembly); if (args.illumina_reads.size() > 0) kmers.add_read_fastqs(args.illumina_reads); } // Read through input long reads once, storing them as Read objects and calculating their scores. // While we go, make sure there are no duplicate read names. Quit with an error if so. long long total_bases = 0; long long last_progress = 0; std::vector reads; std::unordered_map read_dict; if (!args.verbose) std::cerr << "Scoring long reads\n"; int l; gzFile fp = gzopen(args.input_reads.c_str(), "r"); kseq_t * seq = kseq_init(fp); bool any_fasta = false; bool any_fastq = false; while (true) { l = kseq_read(seq); if (l == -1) // end of file break; if (l == -2) { std::cerr << "Error: incorrect FASTQ format for read " << seq->name.s << "\n"; return 1; } if (l == -3) { std::cerr << "Error reading " << args.input_reads << "\n"; return 1; } else { total_bases += seq->seq.l; std::string read_name = seq->name.s; bool fasta_format = (seq->qual.l == 0 && seq->seq.l > 0); bool fastq_format = (seq->qual.l > 0 && seq->seq.l > 0 && seq->qual.l == seq->seq.l); any_fasta = (any_fasta || fasta_format); any_fastq = (any_fastq || fastq_format); if (any_fasta && any_fastq) { std::cerr << "\n\n" << "Error: could not parse input reads" << "\n"; std::cerr << " problem occurred at read " << read_name << "\n"; return 1; } if (fasta_format && kmers.empty()) { std::cerr << "\n\n" << "Error: FASTA input not supported without an external reference" << "\n"; return 1; } Read * read = new Read(read_name, seq->seq.s, seq->qual.s, int(seq->seq.l), &kmers, &args); reads.push_back(read); if (args.verbose) read->print_verbose_read_info(); if (read_dict.find(read->m_name) != read_dict.end()) { std::cerr << "Error: duplicate read name: " << read->m_name << "\n"; return 1; } read_dict[read->m_name] = read; if (total_bases - last_progress >= 483611) { // a big prime number so progress updates don't round off last_progress = total_bases; if (!args.verbose) print_read_score_progress(reads.size(), total_bases); } } } kseq_destroy(seq); gzclose(fp); if (!args.verbose) print_read_score_progress(reads.size(), total_bases); std::cerr << "\n"; // Determine the output format. bool fasta_output = any_fasta; bool fastq_output = any_fastq; // Gather up reads to output. If a read has been trimmed/split, it's these child reads which we use, not the // parent read. std::vector reads2; for (auto read : reads) { if (read->m_child_reads.size() == 0) { reads2.push_back(read); } else { for (auto child : read->m_child_reads) reads2.push_back(child); } } size_t longest_read_name = 0; for (auto read : reads2) { if (read->m_name.size() > longest_read_name) longest_read_name = read->m_name.size(); } // If --trim or --split was used, display some summary info here. if (args.trim || args.split_set) { long long total_after_trim_split = 0; for (auto read : reads2) total_after_trim_split += read->m_length; if (args.trim && args.split_set) std::cerr << " after trimming and splitting: "; else if (args.trim) std::cerr << " after trimming: "; else std::cerr << " after splitting: "; std::cerr << int_to_string(reads2.size()) << " reads (" << int_to_string(total_after_trim_split) << " bp)\n"; } std::cerr << "\n"; // Go through the mean quality scores and find the min, max, mean and standard deviation. double min_quality = 100.0; double max_quality = 0.0; double quality_sum = 0.0; for (auto read : reads2) { quality_sum += read->m_mean_quality; if (read->m_mean_quality > max_quality) max_quality = read->m_mean_quality; if (read->m_mean_quality < min_quality) min_quality = read->m_mean_quality; } double mean_quality = quality_sum / reads2.size(); double stdev_sum = 0.0; for (auto read : reads2) { double mean_diff = read->m_mean_quality - mean_quality; stdev_sum += mean_diff * mean_diff; } double stdev_quality = sqrt(stdev_sum / reads2.size()); double min_z_score, max_z_score; if (stdev_quality > 0.0) { min_z_score = (min_quality - mean_quality) / stdev_quality; max_z_score = (max_quality - mean_quality) / stdev_quality; } else { min_z_score = 1.0; max_z_score = 1.0; } double max_min_z_diff = max_z_score - min_z_score; // Now normalise each read's quality scores. if (args.verbose) std::cerr << "\n\n" << "Read name" << "\t" << "Length score" << "\t" << "Mean quality score" << "\t" << "Window quality score" << "\t" << "Final score" << "\n"; for (auto read : reads2) { double window_ratio = read->m_window_quality / read->m_mean_quality; if (window_ratio > 1.0) window_ratio = 1.0; double quality_z_score = (read->m_mean_quality - mean_quality) / stdev_quality; read->m_mean_quality = 100.0 * (quality_z_score - min_z_score) / max_min_z_diff; read->m_window_quality = read->m_mean_quality * window_ratio; read->set_final_score(args.length_weight, args.mean_q_weight, args.window_q_weight); if (args.verbose) read->print_scores(longest_read_name); } if (args.verbose) std::cerr << "\n"; // If the user set thresholds using either --target_bases or --keep_percent, then we need to see which additional // reads should be labelled as failed. if (args.target_bases_set || args.keep_percent_set) { std::cerr << "Filtering long reads\n"; // See how many bases have already been passed. long long passed_bases = 0; for (auto read : reads2) { if (read->m_passed) passed_bases += read->m_length; } // Determine how many bases we should keep. long long target_bases; if (args.target_bases_set) target_bases = args.target_bases; else target_bases = std::numeric_limits::max(); if (args.keep_percent_set) { long long keep_target = (long long)((args.keep_percent / 100.0) * total_bases); target_bases = std::min(target_bases, keep_target); } std::cerr << " target: " << int_to_string(target_bases) << " bp\n"; if (target_bases >= total_bases) { std::cerr << " not enough reads to reach target\n"; } else if (target_bases >= passed_bases) { std::cerr << " reads already fall below target after filtering\n"; } else { // Sort reads from best to worst. std::sort(reads2.begin(), reads2.end(), [](const Read* a, const Read* b) {return a->m_final_score > b->m_final_score;}); // Fail all reads after the threshold has been met. long long bases_so_far = 0; for (auto read : reads2) { if (read->m_passed && bases_so_far < target_bases) bases_so_far += read->m_length; else read->m_passed = false; } std::cerr << " keeping " << int_to_string(bases_so_far) << " bp\n"; } std::cerr << "\n"; } // Read through input reads again, this time outputting the keepers to stdout and ignoring the failures. std::cerr << "Outputting passed long reads\n"; fp = gzopen(args.input_reads.c_str(), "r"); seq = kseq_init(fp); while ((l = kseq_read(seq)) >= 0) { Read * read = read_dict[seq->name.s]; if (read->m_child_reads.size() == 0) { if (read->m_passed) { std::cout << (fasta_output ? ">" : "@"); std::cout << seq->name.s; if (seq->comment.l > 0) std::cout << " " << seq->comment.s; std::cout << "\n"; std::cout << seq->seq.s << "\n"; if (fastq_output) { std::cout << "+\n"; std::cout << seq->qual.s << "\n"; } } } else { for (size_t i = 0; i < read->m_child_reads.size(); ++i) { Read * child_read = read->m_child_reads[i]; if (child_read->m_passed) { std::pair child_read_range = read->m_child_read_ranges[i]; int start = child_read_range.first; int end = child_read_range.second; int length = end - start; if (length > 0) { std::cout << (fasta_output ? ">" : "@"); std::cout << child_read->m_name; if (seq->comment.l > 0) std::cout << " " << seq->comment.s; std::cout << "\n"; std::string seq_str = seq->seq.s; std::cout << seq_str.substr(start, length) << "\n"; if (fastq_output) { std::string qual_str = seq->qual.s; std::cout << "+\n"; std::cout << qual_str.substr(start, length) << "\n"; } } } } } } kseq_destroy(seq); gzclose(fp); // Clean up. for (auto read : reads) delete read; std::cerr << "\n"; return 0; } Filtlong-0.2.1/src/misc.cpp000066400000000000000000000027621406722713100155300ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #include "misc.h" #include #include #include std::string double_to_string(double n) { std::stringstream ss; ss << std::fixed << std::setprecision(2) << n; std::string s = ss.str(); if (s.size() < 5) return std::string(5 - s.size(), ' ') + ss.str(); else return ss.str(); } std::string int_to_string(long long n) { std::stringstream ss; ss.imbue(std::locale("")); ss << std::fixed << n; return ss.str(); } void print_hash_progress(std::string filename, long long base_count) { std::cerr << "\r " << filename << " (" << int_to_string(base_count) << " bp)"; } void print_read_score_progress(int read_count, long long base_count) { std::cerr << "\r " << int_to_string(read_count) << " reads (" << int_to_string(base_count) << " bp)"; } Filtlong-0.2.1/src/misc.h000066400000000000000000000017211406722713100151670ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #ifndef MISC_H #define MISC_H #include std::string double_to_string(double n); std::string int_to_string(long long n); void print_hash_progress(std::string filename, long long base_count); void print_read_score_progress(int read_count, long long base_count); #endif // MISC_H Filtlong-0.2.1/src/read.cpp000066400000000000000000000236751406722713100155160ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #include #include #include #include #include "read.h" #include "misc.h" Read::Read(std::string name, char * seq, char * qscores, int length, Kmers * kmers, Arguments * args) { m_name = name; m_length = length; m_first_base_in_kmer = -1; m_last_base_in_kmer = -1; std::vector qualities; // If reference k-mers aren't available, use the qscores to get the qualities. if (kmers->empty()) { qualities.reserve(length); for (int i = 0; i < length; ++i) qualities.push_back(qscore_to_quality(qscores[i])); } // If there are reference k-mers, use them for the qualities. A base is considered to have a quality of 1 if it // is in any present 16-mer, 0 if it is not. else { qualities.resize(length, 0.0); if (length >= 16) { uint32_t kmer = kmers->starting_kmer_to_bits_forward(seq); for (int i = 15; i < length; ++i) { if (i > 15) { kmer <<= 2; kmer |= kmers->base_to_bits_forward(seq[i]); } if (kmers->is_kmer_present(kmer)) { for (int j = i - 15; j <= i; ++j) qualities[j] = 1.0; } } } } m_mean_quality = get_mean_quality(qualities); m_window_quality = get_window_quality(qualities, args->window_size); m_length_score = get_length_score(); // See if the read failed any of the hard cut-offs. m_passed = true; if (args->min_length_set && m_length < args->min_length) m_passed = false; else if (args->max_length_set && m_length > args->max_length) m_passed = false; else if (args->min_mean_q_set && m_mean_quality < args->min_mean_q) m_passed = false; else if (args->min_window_q_set && m_window_quality < args->min_window_q) m_passed = false; m_first_base_in_kmer = -1; m_last_base_in_kmer = -1; if (!kmers->empty()) { for (int i = 0; i < length; ++i) { if (qualities[i] != 0) { if (m_first_base_in_kmer == -1) m_first_base_in_kmer = i; m_last_base_in_kmer = i + 1; } } if (args->trim || args->split_set) { // Look at qualities to define 'bad ranges' of the read. if (args->split_set) { int i = 0; while (i < length) { if (qualities[i] == 0.0) { int bad_start = i; while (i < length and qualities[i] == 0.0) ++i; int bad_end = i; if (bad_end - bad_start >= args->split) m_bad_ranges.push_back(std::pair(bad_start, bad_end)); } else ++i; } } // If we're trimming, add the trimmed parts as bad ranges (unless they are already in there). if (args->trim) { if (m_first_base_in_kmer > 0) { std::pair trim_start(0, m_first_base_in_kmer); if (m_bad_ranges.size() == 0 || m_bad_ranges.front() != trim_start) m_bad_ranges.insert(m_bad_ranges.begin(), trim_start); } if (m_last_base_in_kmer != -1 && m_last_base_in_kmer < length) { std::pair trim_end(m_last_base_in_kmer, length); if (m_bad_ranges.size() == 0 || m_bad_ranges.back() != trim_end) m_bad_ranges.push_back(trim_end); } } if (m_bad_ranges.size() > 0) { int range_start = 0; int range_end; for (auto bad_range : m_bad_ranges) { range_end = bad_range.first; if (range_end - range_start > 0) m_child_read_ranges.push_back(std::pair(range_start, range_end)); range_start = bad_range.second; } range_end = length; if (range_end - range_start > 0) m_child_read_ranges.push_back(std::pair(range_start, range_end)); for (size_t i = 0; i < m_child_read_ranges.size(); ++i) { int child_start = m_child_read_ranges[i].first; int child_length = m_child_read_ranges[i].second - child_start; int child_end = child_start + child_length; std::string child_name = m_name + "_" + std::to_string(child_start+1) + "-" + std::to_string(child_end); Read * child = new Read(child_name, seq + child_start, qscores + child_start, child_length, kmers, args); m_child_reads.push_back(child); } } } } } Read::~Read() { for (auto child : m_child_reads) delete child; } std::string pad(std::string s, const size_t width) { if (width > s.size()) return s + std::string(width - s.size(), ' '); else return s; } std::string pad(int num, const size_t width) { std::string s = std::to_string(num); return pad(s, width); } void Read::print_verbose_read_info() { std::cerr << "\n" << m_name << "\n"; std::cerr << " length = " << pad(m_length, 11); std::cerr << "mean quality = " << double_to_string(m_mean_quality); std::cerr << " window quality = " << double_to_string(m_window_quality) << "\n"; if (m_bad_ranges.size() > 0) { std::cerr << " bad ranges = "; for (size_t i = 0; i < m_bad_ranges.size(); ++i) { std::cerr << m_bad_ranges[i].first << "-" << m_bad_ranges[i].second; if (i < m_bad_ranges.size() - 1) std::cerr << ", "; } std::cerr << "\n"; } if (m_child_read_ranges.size() > 0) { std::cerr << " child ranges = "; for (size_t i = 0; i < m_child_read_ranges.size(); ++i) { std::cerr << m_child_read_ranges[i].first << "-" << m_child_read_ranges[i].second; if (i < m_child_read_ranges.size() - 1) std::cerr << ", "; } std::cerr << "\n"; } for (auto child : m_child_reads) child->print_verbose_read_info(); } void Read::print_scores(size_t name_length) { std::cerr << pad(m_name, name_length) << "\t" << double_to_string(m_length_score) << "\t" << double_to_string(m_mean_quality) << "\t" << double_to_string(m_window_quality) << "\t" << double_to_string(m_final_score) << "\n"; } double Read::get_mean_quality(std::vector & qualities) { double sum = 0.0; for (auto q : qualities) sum += q; return 100.0 * sum / qualities.size(); } double Read::get_window_quality(std::vector & qualities, size_t window_size) { if (qualities.size() <= window_size) return get_mean_quality(qualities); double sum = 0.0; for (size_t i = 0; i < window_size; ++i) sum += qualities[i]; double window_quality = sum / window_size; double min_window_quality = window_quality; for (size_t j = window_size; j < qualities.size(); ++j) { size_t i = j - window_size; window_quality -= qualities[i] / window_size; window_quality += qualities[j] / window_size; if (window_quality < min_window_quality) min_window_quality = window_quality; } if (min_window_quality < 0.5 / window_size) min_window_quality = 0.0; return 100.0 * min_window_quality; } // At the moment, the half-score length is hard-coded to 5 kbp. Maybe this should be adjustable via a setting? // https://www.desmos.com/calculator // y=100\left(1+\frac{-a}{x+a}\right) double Read::get_length_score() { double half_length_score = 5000.0; return 100.0 * (1.0 + (-half_length_score / (m_length + half_length_score))); } // The final score is a weighted geometric mean of the length score and the mean quality. It is then scaled down using // the window quality. void Read::set_final_score(double length_weight, double mean_q_weight, double window_q_weight) { // First get the weighted geometric mean of the length score and the mean quality. double product = pow(m_length_score, length_weight) * pow(m_mean_quality, mean_q_weight); double total_weight = length_weight + mean_q_weight; double final_score = pow(product, 1.0 / total_weight); // Now scale that down using the ratio of window quality to mean quality. double scaling_factor; if (m_mean_quality > 0.0) scaling_factor = std::min(m_window_quality / m_mean_quality, 1.0); else scaling_factor = 1.0; total_weight = length_weight + mean_q_weight + window_q_weight; double window_weight_fraction = window_q_weight / total_weight; double non_window_weight_fraction = 1.0 - window_weight_fraction; scaling_factor = non_window_weight_fraction + (scaling_factor * window_weight_fraction); m_final_score = final_score * scaling_factor; } double Read::qscore_to_quality(char qscore) { int q = qscore - 33; return 1.0 - pow(10.0, -q / 10.0); } Filtlong-0.2.1/src/read.h000066400000000000000000000033771406722713100151600ustar00rootroot00000000000000// Copyright 2017 Ryan Wick // This file is part of Filtlong // Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License // as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later // version. // Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied // warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more // details. // You should have received a copy of the GNU General Public License along with Filtlong. If not, see // . #ifndef READ_H #define READ_H #include #include #include #include #include "kmers.h" #include "arguments.h" class Read { public: Read(std::string name, char * seq, char * qscores, int length, Kmers * kmers, Arguments * args); ~Read(); void print_verbose_read_info(); void print_scores(size_t name_length); void set_final_score(double length_weight, double mean_q_weight, double window_q_weight); std::string m_name; int m_length; double m_length_score; double m_mean_quality; double m_window_quality; double m_final_score; bool m_passed; int m_first_base_in_kmer; int m_last_base_in_kmer; std::vector > m_bad_ranges; std::vector m_child_reads; std::vector > m_child_read_ranges; private: double get_mean_quality(std::vector & qualities); double get_window_quality(std::vector & qualities, size_t window_size); double get_length_score(); double qscore_to_quality(char qscore); }; #endif // READ_H Filtlong-0.2.1/test/000077500000000000000000000000001406722713100142525ustar00rootroot00000000000000Filtlong-0.2.1/test/README.md000066400000000000000000000005121406722713100155270ustar00rootroot00000000000000# Filtlong tests Filtlong comes with a few automated tests to help with development and spotting bugs. Unlike Filtlong itself, which is written in C++, the tests are written in Python3, so you'll need that installed to run them. To run the tests, execute this command from Filtlong's root directory: ``` python3 -m unittest ``` Filtlong-0.2.1/test/__init__.py000066400000000000000000000000001406722713100163510ustar00rootroot00000000000000Filtlong-0.2.1/test/make_synthetic_reads.py000066400000000000000000000125501406722713100210140ustar00rootroot00000000000000''' This script was a quick hack to make simulated Illumina and PacBio reads for circular sequences. PBSIM and Wgsim do not seem to have a 'circular reference' option, nor did it have an option for different depths for different references. This script simply runs PBSIM over and over, each time randomly rotating the reference sequences, adding all results to one file. It's pretty crude - doesn't take command line arguments but instead you need to set constants at the top of the script. ''' from __future__ import print_function from __future__ import division import random import os import subprocess # INPUT AND PARAMETERS source_fasta = 'test_reference.fasta' base_depths = [1.0] # One for each sequence in the source pacbio_depth_factor = 30.0 illumina_depth_factor = 40.0 start_position_count = 10 # The number of times to run PBSIM and Wgsim with random start positions illumina_read_length = 100 illumina_fragment_size = 350 illumina_fragment_std_dev = 50 pbsim_command = '/Users/Ryan/Applications/pbsim/pbsim' wgsim_command = 'wgsim' # OUTPUT pacbio_reads_filename = 'synthetic_pacbio_reads.fastq' illumina_reads_filename_1 = 'synthetic_illumina_reads_1.fastq' illumina_reads_filename_2 = 'synthetic_illumina_reads_2.fastq' # Global var: pacbio_read_counter = 1 def main(): names, ref_seqs = load_fasta(source_fasta) pacbio_reads = open(pacbio_reads_filename, 'w') illumina_reads_1 = open(illumina_reads_filename_1, 'w') illumina_reads_2 = open(illumina_reads_filename_2, 'w') for i in range(start_position_count): for i, _ in enumerate(ref_seqs): generate_reads(ref_seqs[i], names[i], base_depths[i] / start_position_count, pacbio_reads, illumina_reads_1, illumina_reads_2) pacbio_reads.close() illumina_reads_1.close() illumina_reads_2.close() zip_file(illumina_reads_filename_1) zip_file(illumina_reads_filename_2) zip_file(pacbio_reads_filename) def generate_reads(sequence, name, base_depth, pacbio_reads, illumina_reads_1, illumina_reads_2): global pacbio_read_counter # Randomly rotate the sequence and save it to file. random_start = random.randint(0, len(sequence) - 1) rotated = sequence[random_start:] + sequence[:random_start] rotated_file = open('rotated.fasta', 'w') rotated_file.write('>' + name + '\n') rotated_file.write(rotated) rotated_file.write('\n') # Generate PacBio reads with PBSIM pacbio_depth = base_depth * pacbio_depth_factor command = [pbsim_command, '--data-typ', 'CLR', '--depth', str(pacbio_depth), '--model_qc', '/Users/Ryan/Applications/pbsim/model_qc_clr', 'rotated.fasta'] print(' '.join(command)) process = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE) out, err = process.communicate() print(out) print(err) pbsim_file = open('sd_0001.fastq', 'r') # Copy reads to main file, but add the cycle number to the read ID so we don't end up with # multiple reads with the same ID line_num = 0 for line in pbsim_file: if line_num % 2 == 0: line = line.strip() + '_' + str(pacbio_read_counter) + '\n' pacbio_reads.write(line) line_num += 1 if line_num % 4 == 0: pacbio_read_counter += 1 # Generate Illumina reads with Wgsim illumina_depth = base_depth * illumina_depth_factor illumina_read_pair_count = int(illumina_depth * len(sequence) / (2 * illumina_read_length)) command = [wgsim_command, '-1', str(illumina_read_length), '-2', str(illumina_read_length), '-d', str(illumina_fragment_size), '-s', str(illumina_fragment_std_dev), '-N', str(illumina_read_pair_count), 'rotated.fasta', '1.fastq', '2.fastq'] print(' '.join(command)) process = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE) out, err = process.communicate() print(out) print(err) wgsim_file_1 = open('1.fastq', 'r') for line in wgsim_file_1: illumina_reads_1.write(line) wgsim_file_2 = open('2.fastq', 'r') for line in wgsim_file_2: illumina_reads_2.write(line) # Clean up os.remove('sd_0001.fastq') os.remove('sd_0001.maf') os.remove('sd_0001.ref') os.remove('1.fastq') os.remove('2.fastq') os.remove('rotated.fasta') def zip_file(filename): command = ['gzip', filename] process = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE) _, _ = process.communicate() def load_fasta(filename): # type: (str) -> list[tuple[str, str]] ''' Returns the names and sequences for the given fasta file. ''' seq_names = [] seqs = [] fasta_file = open(filename, 'r') name = '' sequence = '' for line in fasta_file: line = line.strip() if not line: continue if line[0] == '>': # Header line = start of new contig if name: seq_names.append(name.split()[0]) seqs.append(sequence) name = '' sequence = '' name = line[1:] else: sequence += line if name: seq_names.append(name.split()[0]) seqs.append(sequence) return seq_names, seqs if __name__ == '__main__': main() Filtlong-0.2.1/test/test_bad_fastq.fastq000066400000000000000000000725371406722713100203130ustar00rootroot00000000000000@test_good_1 AGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCCGAATTCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATTTTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAGTAACGAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTAAGGACTCCCCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAGTGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTTCAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGATTCGCGCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTAGGGGGAGCGCCCTGAGGAGAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCGAGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCCTACCGGAATTCTCATGCACTTCTGCCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGCCCAGGATATGGAAGTTAAGGCTGGAGGAATAGATAACCGTCGTACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCTGATAATGTTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACTCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGGACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATCCCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACCAATCGACGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCGTTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACGATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGGCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATACACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGAGTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGTTAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACCCCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAAATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCAAGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTTAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACGAAGGCATGTCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT 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 @test_good_2 AGCGGGACTCTTCCCGATCGGCCTGGAAAAACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTAGAAAAGGTAGCCAGCGAGAAGGAAGAAGGCCAAGGCTTCATATTACCCCGAATTCTCAAGGTGGATAAGGGGAACTAACGGTTGCGAGCATGAAGCTGGCTTCAATGGCCAGGCGACAGATTTACTCCACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCGGGTTAGCACTCCCTAATTTTATCCAAGTAGGCGGACACACCCTTACCACAGGGGCTCCAATGTTCGCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTCTGCTGACGCGAAGTAACGAGTATTCTCTTGGATTTTAACTGAGCTGTCACTCCGCGTGAAGGATAACCGGCTTGAGTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGATTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTAAGGACTCCCCCCTGGCGTCGAAGCCAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGGACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTCTCGTGTGAGAGATTAATGTTAGCCCTCAGTGGGTCCCAATAAGAACCCACTAAGTAAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCGGCATAAGGAGAAACGTTATCGGCTGTCGGTGTCATCTGCCAGTGCTGGCGAGTCGAGCTTGTAGCACTCATTGAAGGTGGTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGCGCCTTCAGTGCACTGTGACAGCTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGACGCGCTCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTAGGGGGAGTGCCCTGAGGAGAGTGGAGCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACTAACTGGGGCCGTTGGGCTCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCCAGGCTGCTCAAAAGTCTGCGTAGGAGTCTCATCGGGACCTATACTAGCTGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGATTTACCTATGGACATGCGGTGTCCTAATTCATCCTACCGGAATTCTCATGCACTTCTGCCTTTCCCCCGAACATTCTAGTGGTGGGCTCGAGCTGCTGATAATCCACTCCATTGGGTGATACGAGAGGCGAGTTTGCCTTAGTTTGCGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCCGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCACGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTCTTGACGAGTGTGGGCCCAGAAGATGGAAGTTAAGGCTGGAGGAATAGATAACCATCGTACCTCCACTGGACGAGATAGCACTAAATCCTGGAAACGAGACGGATCCGAGAGTTCAGATAATGATCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAATTATAACGTCGCATACCGATTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAATCCCTAGTTCGGAAAATCGTGTCGCCCCTACAGTCCATCACTCAACTTCACTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGGACCCTTGTGTGGATCGTAAACCTACAAGTCCCTGGCACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACTATCCCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACGAATCGACCGCTCTAACTTTTGAATCTGTGGCATGGACCCCATCAAGAGGGACCAGCGTTCAGCGAGACTTCTACAAACTTACGTTACATTATGCCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACAATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGACCTCATATAGAATGTGCAATCGCTCTTTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTTCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTGTAGCCTCATGACTGCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGTCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTATTGAACCACTGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACCATTGAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGCTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGATGGTATAGGAGACATGCCTATACACGTTCGAGTGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGCGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACTCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAAATTAGGAGTCATGAGTCCGGATTGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACGCGTCAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGCTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAAAGCACCCCTATGTGTTGTCGTTTGGGCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATGTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCATTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAAAGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAGATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGTTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGTATTCGTTCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCTGAGCCACTATAAAATCAAGTGCTCTTATGATAGGGATTGCCAGGACGTTTGGAACTGGCTCAGACATTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGTATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTAAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTCTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAATGGAGCCGGACGAAGGCATGGCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGACGCACCGATATTTGGTACTTTTCTTGGATTATTAGCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT + 64466465654456545455456445454545456444644455645446566655656445666645546565456466454564454554456544555654546566544555564665546446545454546465566664456564445644644666666654454466464546466646445556655564465455664666566656555646446456466655656565646645645555455565656656454646464464554546455464466445544456454445554566445564656546566554555456446445464655655464545655664545654464445464554564556455666656444456456654445544566564646564464564544456465445564446455656645646566554544645646566466644444564464555566544464444664544556444655444646546456645546645464645444456656564645654465656556666465556564664455446646545666666564545454665465454454566564565645666554554655655664554566446546646556655555565654465545464444646654446564555554564556566465546566564554554545455644566544545666645566556444654645455464555646464556645546665555566556544555444554666544664665544464664444444544566466644466564645445545544446546465455466566656455446544564456565445564566646566645466445656454465664556565566665666565446456566455664646455646545564446554646545665655545464665455565664646556654645644456546564654554644554545456565664466566664644565466454455455645545655644565666464554444646546464646454446445544544446466445555444455544555654646455664665544655654556666444446555454654665455645655645554445446645454465455466546566666554464454665456456445556466656465564566666666454666555666644545445544456656555465664456454666644454465464654654566545555445565455464544644646445665666456656544664556454655566465446464454464654454665456565566546545546666554445444454564555564665555444556444545654646656566455545554664455546656546666444455664455664444565646656466664555546456565455564466645566454556455665556665656444466665564656444444445654465645664644464565446446656455644555664444654466565465455554665464644444564664565564445444554645656565446546544554554556654665566446644554546646666445654455464556646555655646664456656646556654455455665545545655466555455446565546454564565654655556555446646465455664565556565554445444464454665556664445646456464456565664465456656465666454455446445455455445454665545665446664456646666664544556555655656554444555656654665544446555455464645564455554644556444545555666645446454646455555654465654454444566464645446454654545546565465555444465555656556555566565445545464564556464564646556466464665454556555466566545454654554566456454455544566446454455645646554454446646644445455446665466464544655445646645456446445556455565655465545454566566466465454654564444666455456545555454654446545654654555466644444644665565664465466656556654455655565544644444566446444546646665464546654546566655646546654644546545466656554666464666465656465644545456456644655545664554645656445656666545545554566666656664544544654554644646664445646445466646554444565654566446646664454544664566666555646656555556445446646556566446555545545646646664544545466556645554445565564664546656465545454545564654665454446566556465644564644564655545565465455664545655544464564445554456444545654666446545566454546556464444444466545555564446555655655564445665556644564465564464444546655544546565456545555555566645544656444564655546445465555645456546654654645464466565644454554666465546544464565644656666545564664554654545554545544656656555654544644464444466646455655444564454556556556665655465565544566545665464644465656554556565544444456656656665566655656445456544546644656545566465545644465545445565445554546464555665666544565466665665655456544464444454654444656654465664666556544664665566565565666456465554455455544445646655456455456566446544445466445455656655566664465646666444446654664464454554646565544644665556454656455465644564465455455444545566446665565644466546445665444454666544645554646664666554554446545644665665645544445646444654666645456445545456446656566446456645566665666454656564554555545466654565464554665556554566456445566555555665656665454565664664556556544554545646656554545445645655545554654644444556566555666664454546545644644454446546446546645544456464655444544566644654546645566444466666654654554464555444564446544654655465545464645566555564466444654465646466455646444555566566544644545466465446464664666646644545664545455444645656666656655565446644446445555445456544446656644465564654654466644655466556445656654565656655446665445654656446466556546554445566446446566555465454446446646556545656546464664466445564446455565566564456544564465646565456545464566666654465544666446546466456444454566666665465556664564544664544465465645646454655646454455664655466456645646464464444544445545655655665546466564564644565555564665655664466556566555566656566545445555564444665444455456545646655546654564446645545445445665664444664666565464646665546446656555644456555645666445646456455466555546545656654566644566556666544666564464665454554664565456664656564656545445465465645454665666654556454566655556446464556564544446546644644465655545645565456445456656666565655554466555654544655644544444545466556644664566445464655554446566666464444656444666546665555466564555554446565646455544544555666545556445554565655566446456665645464454656 @test_bad AGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCCGAATGCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATTTTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAATAACGAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATAGACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCATATACAGGGTTGTGCGCTCGTAAGGACTCCGCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAGTGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTTCAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTATGATTCGCGCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTGGGGGGAGCGCCCTGAGGAAAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCGGGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCCATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCTTACCGGAATTCTCATGCACTTCTGCCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGTGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGCCCAGGATATGGAAGTTAAGGCTGGAGGAATAGTTAACCGTCGAACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCTGATAATGGTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTGAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACCCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGCACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTTTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATCCCTCAAATCTCGTTGATGAACCCGCCCCTTCTGTTCCATTACCAATCGATGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCGTTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCCCGGTGAGCGTGCGGTCACGCATTCCAAAAGTACTCCGCGAGCCCTACGATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCGACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGGCAGGGCGTCTTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGAGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATACACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGAGCGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATCATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGATTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGTTAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACCCCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATTTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACACTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCATGCCGTGATCCGCTTACCAAATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGGTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCTAGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACCTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGCCCTTAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCTACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACCAAGGCATATCGACAGATATCTATCACAAGCCAAATACGCGGGCTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT + 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Filtlong-0.2.1/test/test_error_messages.py000066400000000000000000000221651406722713100207110ustar00rootroot00000000000000""" Copyright 2017 Ryan Wick (rrwick@gmail.com) https://github.com/rrwick/Filtlong This module contains some tests for Filtlong. To run them, execute `python3 -m unittest` from the root Filtlong directory. This file is part of Filtlong. Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with Filtlong. If not, see . """ import unittest import os import subprocess class TestErrorMessages(unittest.TestCase): def run_command(self, command): binary_path = os.path.join(os.path.dirname(os.path.dirname(__file__)), 'bin', 'filtlong') input_path = os.path.join(os.path.dirname(__file__), 'test_sort.fastq') input_fasta = os.path.join(os.path.dirname(__file__), 'test_sort.fasta') bad_fastq = os.path.join(os.path.dirname(__file__), 'test_bad_fastq.fastq') assembly_reference = os.path.join(os.path.dirname(__file__), 'test_reference.fasta') illumina_reference_1 = os.path.join(os.path.dirname(__file__), 'test_reference_1.fastq.gz') illumina_reference_2 = os.path.join(os.path.dirname(__file__), 'test_reference_2.fastq.gz') command = command.replace('filtlong', binary_path) command = command.replace('INPUT', input_path) command = command.replace('FASTA', input_fasta) command = command.replace('BADFASTQ', bad_fastq) command = command.replace('ASSEMBLY', assembly_reference) command = command.replace('ILLUMINA_1', illumina_reference_1) command = command.replace('ILLUMINA_2', illumina_reference_2) output_name = 'TEMP_' + str(os.getpid()) command = command.replace('OUTPUT', output_name) try: self.output_file = [x for x in command.split() if output_name in x][0] except IndexError: self.output_file = '' p = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) _, err = p.communicate() return err.decode(), p.returncode def tearDown(self): if os.path.isfile(self.output_file): os.remove(self.output_file) def test_empty_command(self): """ If no arguments are given at all, it should display the help text. """ console_out, return_code = self.run_command('filtlong') self.assertTrue('usage:' in console_out) self.assertTrue('Filtlong:' in console_out) self.assertEqual(return_code, 0) def test_no_thresholds(self): console_out, return_code = self.run_command('filtlong INPUT > OUTPUT.fastq') self.assertTrue('Error: no thresholds set' in console_out) self.assertEqual(return_code, 1) def test_input_reads_do_not_exit(self): console_out, return_code = self.run_command('filtlong --target_bases 1000 BAD_FILENAME > OUTPUT.fastq') self.assertTrue('Error: cannot find file' in console_out) self.assertEqual(return_code, 1) def test_reference_assembly_does_not_exit(self): console_out, return_code = self.run_command('filtlong -a BAD_FILENAME --target_bases 1000 INPUT > OUTPUT.fastq') self.assertTrue('Error: cannot find file' in console_out) self.assertEqual(return_code, 1) def test_reference_reads_1_does_not_exit(self): console_out, return_code = self.run_command('filtlong -1 BAD_FILENAME -2 ILLUMINA_2 --target_bases 5000 ' 'INPUT > OUTPUT.fastq') self.assertTrue('Error: cannot find file' in console_out) self.assertEqual(return_code, 1) def test_reference_reads_2_does_not_exit(self): console_out, return_code = self.run_command('filtlong -1 ILLUMINA_1 -2 BAD_FILENAME --target_bases 5000 ' 'INPUT > OUTPUT.fastq') self.assertTrue('Error: cannot find file' in console_out) self.assertEqual(return_code, 1) def test_target_bases_too_low_1(self): console_out, return_code = self.run_command('filtlong --target_bases 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --target_bases must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_target_bases_too_low_2(self): console_out, return_code = self.run_command('filtlong --target_bases -10 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --target_bases must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_keep_percent_too_low(self): console_out, return_code = self.run_command('filtlong --keep_percent 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --keep_percent must be greater than 0 and less than 100' in console_out) self.assertEqual(return_code, 1) def test_keep_percent_too_high_1(self): console_out, return_code = self.run_command('filtlong --keep_percent 100 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --keep_percent must be greater than 0 and less than 100' in console_out) self.assertEqual(return_code, 1) def test_keep_percent_too_high_2(self): console_out, return_code = self.run_command('filtlong --keep_percent 111.1 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --keep_percent must be greater than 0 and less than 100' in console_out) self.assertEqual(return_code, 1) def test_min_length_too_low_1(self): console_out, return_code = self.run_command('filtlong --min_length 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --min_length must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_min_length_too_low_2(self): console_out, return_code = self.run_command('filtlong --min_length -10 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --min_length must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_min_mean_q_too_low(self): console_out, return_code = self.run_command('filtlong --min_mean_q 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --min_mean_q must be greater than 0' in console_out) self.assertEqual(return_code, 1) def test_min_window_q_too_low(self): console_out, return_code = self.run_command('filtlong --min_window_q 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --min_window_q must be greater than 0' in console_out) self.assertEqual(return_code, 1) def test_trim_no_reference(self): console_out, return_code = self.run_command('filtlong --trim INPUT > OUTPUT.fastq') self.assertTrue('Error: assembly or read reference is required to use --trim' in console_out) self.assertEqual(return_code, 1) def test_split_no_reference(self): console_out, return_code = self.run_command('filtlong --split 250 INPUT > OUTPUT.fastq') self.assertTrue('Error: assembly or read reference is required to use --split' in console_out) self.assertEqual(return_code, 1) def test_split_too_low_1(self): console_out, return_code = self.run_command('filtlong -a ASSEMBLY --split 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --split must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_split_too_low_2(self): console_out, return_code = self.run_command('filtlong -a ASSEMBLY --split -10 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --split must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_window_size_too_low_1(self): console_out, return_code = self.run_command('filtlong --min_length 1000 --window_size 0 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --window_size must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_window_size_too_low_2(self): console_out, return_code = self.run_command('filtlong --min_length 1000 --window_size -10 INPUT > OUTPUT.fastq') self.assertTrue('Error: the value for --window_size must be a positive integer' in console_out) self.assertEqual(return_code, 1) def test_fasta_input(self): console_out, return_code = self.run_command('filtlong --target_bases 1000 FASTA > OUTPUT.fastq') self.assertTrue('Error: FASTA input not supported without an external reference' in console_out) self.assertEqual(return_code, 1) def test_bad_fastq(self): console_out, return_code = self.run_command('filtlong --target_bases 1000 BADFASTQ > OUTPUT.fastq') self.assertTrue('Error: incorrect FASTQ format for read' in console_out) self.assertEqual(return_code, 1) Filtlong-0.2.1/test/test_reference.fasta000066400000000000000000003052231406722713100202740ustar00rootroot00000000000000>reference TGTATACACAACGGCGTCCCCGGTCTAGTAATAGAACCAACCTGTTCTACTTTAGAATACTGTGTCAATTTCTAACACCAGTTTGAAGTAACGAGAGCTT CCATTACGCATCTGCCGTCCGCCTACCGAACCACATTGAATATCGGCACCGAGTCGCCTATACTTGATTCCGAGTCATTACGGGGGCATGTCGTCAAAAA GGCCGGTCGCTCCTAACACCCGAGAGCTTACTATTTCGGATACAAACTTGGAACATACTGCAGGTGCTTCTTTCTGAGAATCCGTTTGTCATTTACCGCG TACGTGGCTAGTTATCAAGACAGGTCAAACCATGAGATGCATTCCGGATGCGTTACCGGTCCGACAACTATCGCGTCACAATCGCTGTTGGTGCTCTACA CAAACTGTCATCGATATTCTGCAACGACAAGTCGACGTAGCCGGTCATGTCGAGAACTACGCTTAGTTACGAGAGCTGGATTGCACTACATAAGCGAGGA CGCCTAGGTACAGGTGTACGGCACCCGATTGACCAAATCATGCCTAGGAGGCCATAGTTCCGGCGCTCGAATTCCAATTTATTTGTCCTAAGAGCCTGTC TTGTTTAGTGGATACTGTCGACCCAGCAGTTTATGACTAGCTACTGAGGGAATGGGCTAGCATCGTGAATTTACCGCAATCAGATAGAGTCACATCAGTG GTCCGTAGTGTCATACCATTGAACCAGGGTCATACTTGTGGAGGGAAGCAATCTAGGGGCTTAGACCGCAGTAGCCATTGTACTCCGGCGAGTAAGGTAC GCTAATAAGGCCTGTATCTTTGAGGCTTGAAGGTAGGAGGACAAAACAACGCCAAACCTGGTGGTAATGCAATTTCCACCATGGCGGCGGTCGACGAGCG CTATATTGTACCGGCCGACGTCGGAGGTTTAGTATCAGTAAAAGATCGGGGCACCCACTGGTCCATGCGACTTCGCTGTCTCTGGGACTGTTGTTACCAC CAGCGTACACGGGCCGGGCCGCGGGATGAATTTTTCCGCTCAGTCTCTCGCGAACCGCTTGCTTGTCAATTATCACCCTTAATGATTGCCCTGCATGGTA ATGACACAACACAAACAATTACACGGACCACAAGAAGAAAATGGTAGGTACGGTGTTCAAGCGATATCCGATACAACACCGCTTGACCAAGAGCAGCCAA TGAAGCTATTCCAATATTGAACTTTAGAAAGATCAGAGCAACCCTATGACGGGATGCGCCGCCCTGCGTTACACTCGTAGAATAAGAAGAAGGTTTTACT TGTGACTTGTGTGGGTAAATATCGTGTCTACTCAAGAGCACCGTGAGCGAAAAAAACGGATTCCGCCAGCTTTGACGACCGCGGTCCAACGATTTTTCCC AGTGCACCATTACAAACAACCTTATGTAACGATCGAATTTACGGCGCAGGAGTTTATATGACGGTGAGACGCGGTTTACGGTTTTCACCGTGGACAAGGG GATCTAGAGCAATGATTTTCTTGCGGTGGGTTCCGCAGCAACTCTGTCAGTACACTGTGTGTTTCTGACTCCGTGCCAGTATCATTTCAGGCCCGGACTG AGACTGACTTGACAGCATGAGTGTTAGAGCTAGACATGTAGCACAACTGAAGCGTACCCATTCTACCATATTTAGTCAATATAGAACCACGGTGAATCTG AGTCCCCGCTACACATTAGTCATGTCGGTCTACTGGCTGCGCTGACGGTCTAAGTCTATTGAAACGCTTTAAGAGTAGGCGTCGCGGGAGGCGGTGTCAG TCACGTACTCCTAGTGATAGCGCTTGAGGGGCGCGACCCAATACGCCTCATCTAGATAGTCCTCGGGAGCGATGCTCTCAGCTAAGCTCGGGGACGCTAG CTTTTATTAGCAATAAATCGTCGTGACGTCGTCGTGGAAGGCAGACATGCGCCACCGCACTTTAGATAGCCGGAAGTGCGTACCGACTATCCCAATGTTT CATTTTTAATACAGGTCTGTCATAGGCCAGTCGTGATAACTGCTGATTCGCCTCGTAGTAACACCTGCAGTAAGAATGGGTTGCAAAGGAAAAGAAATCT AGACTACGCTCGATACGGTAATCCAACACGTCTTGCCATCACCTAAGCGGGCCGCCATTGAATTCCATCCCCCGATCTCGCGAGCCGTCAGGTCGTTGAT ACCAGAACGCTATTTACTACCGGAAGTGCAGACAACGTTCCACGCGCTTAGGGAAGCTCCTTAACCGCCTTTCCCCCGAGGGTATCGGATTACCGTTCTT TCAGAATAGCCATTTAGCGAAGAACCCTGTTATGGTCTGGATCTATCTGGGCTGCCAATTTTTCGCACATAACACACGTACTTGTCGCTGGGCTCGGCGT TCGGCTATTTATTGTTACTTACGGCAAGAATTTTGGTTTCTGACAAGACTAGTTCCTTTGCGTAGTACAGCAACTACGAGGGTTCTACGGCTTGTGATGA TACAATCGCGATCGACGCTCGGTACGGAATATCCTAACTGCATCTCCGTCAATTCTAGAGCCTGCGATATTAACTACAGTGCTTCCTGTATACACTACGC GCTATAGTTGGTATGAAATTAGACCTTCATGCGACACTCTGCCCGGAAGAGGCGGGGACCTGGACATGATCTGAATCGACATCCAATGCTGTCATGAACT GCTAGAAAAAAGTATAGTCAACACAAAAATAACAGCCCGTTTTTTGACCTATGGGTGATTGCTGACCCTAGGCTAGGAGTGGTCCCTCCTCAGCCTAATA TATGCTTTAAAACTAAACGCACGCCTATTTCATCGCCATTTAGTGATACAGGTAGATAGGTCTGCCGAGAACCATCGAAGCAGGGACCTGGATGTGTGGC GCTTCACGGACAAAGGGGGTGAGCGTTCAGTAGCTGCATTGTACTTAAATGTACGGAGGTACAACCGTTTTTCCTCCATTATCTGTGTGGGCGAGGATCT AGCGTGGGGACCGCCATAGAACTAGAGAATGTCACTCGAGGTACCCTTGCCCGCATTACCCCACGTCGTAGGTCCTTGTTCACACCGGCGTTTCGTCACG GTTCCTTACGCAATCGGTGTTGCTCGCGGCCGCTGCGCTTCATATAGGTGGGCACTCGTACTAATGTGCCTCTAGTTCGTATGTTTTTCGCGTAATATCC GTGGTCCGCCCCAGGATGATAGCTGCTCAGGTCCCTTCTAGAGGGCTGTCCCTCTTGTACTAATCGGTGTCTTCTAGCCAATATCGTCTATTACTGCCGC CGACAAGCGACCGTTCTTGTTTGACGGACCGGTAGTTAGTAACCCCCAGGAACCGGCTCCTGAGTACGGCTGCTAACAGAAGCGCACCACATGATATGTA CATATCTTTGCTATACCGAAAGTGGCCAAACACACATCGGAGTTACGGGATCGTGTACTAGCCCATGCGGTGTCAGGATAAAATCCGAGTACTACAAAAG CCAGGGTAGAAAAGGGGGGTGGCACTAAGCTTCAGTGAGAGTTCTGAGCTTGAGCCAGGGTTTTGTGAGAGTAAGATTCCATACAACCACGCCATCTAGC TGTGCCGGTACTAAGTAGGTCCTTTGCCCACCACACTCGCCGGAAATCCTTAGTGGTCTGGCACTTGTGAAGGATGCATGCTGGAGACTTTACCTCGCGA GCGTGTCCGCGGGCGTCTTTTTGTATAGGATCGCTAGAAGGCGCACACTCAATACATGCTAGACTTTGAGATACGCCGACGGAGAACTCTCTAGTCTTAG ACGATAGGTATAGTGTGCTAACTGTGCCACGGGTCTTATTTTGGTTATCAATATATGACCCTCGCATGAATGCGTTGCTTTTTGCAACTCCCCTGAGCTC TTCAAGTGCGCGTTATCGTCACGTAAGGTGAGAAACTCACAAGGCAAAGGGGTATAGAGCCGTAGAGAAGAACAAGTTGCATTCCCGGACTCGAATATGG GTACTACAGTACCAAACTCCGTGAAATGGATCGACCGAGGGTATAGGAAAGCGGCCATGCGATCGGCGCCACACCGAATCGTGACATGGAAGGAGCGCTC CAAGTCACGGTATGGGAATTCAGTTAACCGATCTCAGCGCAAGTGTTCTCAAACTTACACGTGGAGCCAGGCCTGGTCAGTGAGACAGAACTCTAAGTCA CAGAAGCTATAACATGGATGGTTTAGCGCATATCTCCTAGTATATTGGCATTACGTTATCAGGACCACCTTACGGTACCCCGGAGTGTGTTAGAGGAAGA ATTGGCATAGTTCCCGACCATCAAGTAGACAGCCGGACGCCAATAAACTAGAACTCACATTCCTTGTAAACACACGGACCCAGGACTACATTGAACCGGG CAGACGCAACTGCGAGTCCCCCCCAGCCAGAAGACACAACCTTGACCTGGAACCACCTCCGGTCTGCCCCAGCACCGCAATGCAAGACGAGCTGTAAGTT CAGGTCACTATTTTCTACCCACGCTCGCCGCGCTGTCAGGTCATAGTTCCAGGGTACATGGGGGTATTTGTCCGCGATGGAAGCTGGAGTATTTGACCCC GCTTTTAACTCGGCATGGCAATCGCTCATATTAATAGCCGGATAAGAAGGCACTTAAGGTTTACTGGCCGAACTTACTCGTATTTTGCGATTTTTAGCAA AACGCGCTGAATCGTGTTGTTTCACCGTCGTTGGGGCGTCCCTTCAAGAAGCTCACATGTAGCGCGGGTCTCTCCAGAAGATCGCTCTAAAAGAGCTGCA AACAAGGGGAGAAAGGGCCAGAGTCTGCTTCTCGGCTCATCTCCGTAGGAGCGATGTCCCGTGGGGTGCGGTATATGAGTGCCCCACAGAACCCTAAGGT TGTTCACGAGCTTCGGTGCTTTCCAACCAGTTCCTTACAGTAGGTCGCAGATTACAATATTGTGCACTGCAAAACCTCTTAGGCGCGATTAAGTTCATAA GCAATTTTTTCGGGCTCATACCCGCCGGGGGTAGTGACCGTATTTAATCCTATAGCTGCTGCTTCCACTGCTGGCTCTTCCAGATGACCGATCTCAGAAC CTATTGCCACGTAGCTGTAAGAGTGTACAGACGAGCGCGAACCGTTAACATTGGCGCGAGAGCACAGTCTGTGAAAGCGAAATTTATGTAAGTCACTAAA AAACCAAAAACCAAACTCGACCAGCAAATCCGCCGGACAGTACAAGAGTACCGTCACCTTTATCCCTGCCAGCATCTCTGCATCCTCATAAACTCCAATT TAACGCCAGCTTTGCTTGGCAGTTGGAAGGCGTTCGTGGAAGTCGAGATCGTGTCTTAGGAGGACGTGGAAAACGAAAACCTGCATAGATAACATAATAC GCCCCCATTCTGGACATGCCGCATCCAATCACCTAAGCCGCTGGAGCGAGTCCCGTAGATCTCTGCGTTCTTTTCTCGCTATAGCATAGGTACGCATAAT AGCGCTTTGTTAGTAGTGTGCATAGCTAAGCAAATATGGACACACAGATGTGCGATAGTTAATTAGGACCGCGTTCTGACTCGGCAGGATTTCATGATCC TAACGGCTTCCAGGCTCGGGAGCCTTAAGTATCCTTAGGCACCATTTGCCATACCATTTTCTTATTCGATGCATACGAGCCGAATGTATCTCAACGCTTG AAGGTGCAATTATCGGCCCCCTGGTATCTAATCGATCATTACTACAATTGTACCCAGTCCCCATGCTATACAGTAGGTGACATGTTCTCCCAACACTGTT GACCCTTGGCTGTGCCCAATGGGGGTCTTAACATACATCTAAACAAAATCCTTAAGGGCGTGGAAAATCGCTTGTAAATTTGACGAATGCAGGATACTTG AACGGAAGGTTATAGCTACAAACCCTCAATCAGACGGTATACCCTGTGCCACCTCATTAAATGGTTGGACTTATCTCAATAAACGGCTGCTTGTGTCATG TCAAGACCCTCGATTTTGGTGCCCCGACCCCCGCCGGATTCGAGATGGCGAACGGTGTCCTGCCAGTCCGCGACCTTGCACTAGCCTTTGTGACAATATA CTACCCGTGCGGTTGGCGAAGGGTGTTATGAGGTTAACATGCAAGGCCTCGGATGAACGGCGGCAGTAAGCTGACTCTCGCGGACTGTCGTCCTCCTCTC CGGGGTTCCTCAGCGCTGGGGGTATCCCCACCCGGGATCGTTATGCTGTGTTCTTGCGCGGCCACACATGTAGGCGAAATAGCACCAGGTATATGCGTGA CGTCACAATTGATGCCGGCGGTGTTTCGTCCATTATGGGAATCTCGGGACACAGGTACGTATTACCTACCGTAAGAGGTATATTGCGTTATTGAACCCCT ACATCTGCCACGCCGTGGTCATTTGAGATCACTGCCCGAGACTACGCGTAACCACCTTGTTTAGCCGGATACTCTAGTAAACTTCCCAACGCGACCGCTA GCAGAGGGCTCGCTATGGATCCTAGATAGAAGTTTGTCAATTCTGATCAGAGCACACCACCGAGGCAGTAAAACGGCGTTCATTTTACCCGCATCATATG CGGCAGAGGATAGGCGAATGTAACGCATGTAAGCCGAAACGTATGTCGAGCACCCTGGACATTCCGGGCTACATTCATCATATAGGCCTGACCTGGTTCG AAAGGAAGGGCGGCGGGGGTCCTATGTTTGACCAACTAGAAGCAAATGTGATGCTCACTACGGTATTTATATATATTCTACAACATTAGTCTGGGTCTGG GGTATTCCACTAGCCAAACCCCAATCAGTGCGTATTAATGATTGTGATCGTCCACCGTCGGCGGTACGAGTTCTTTTAGATTTCGTCTCTCAGCGGCGAT GTCAGGGAATACATTGTTACCTATCACTTGTATAGGTTTTGCGCATAGGTCAATGCCTTGATCTGTACCAGGCGTCCGTGCTAGGTATAAATTGCGCGGT GTTAATGTTCTGAGGATTGACGTCCGAGACTATCATGGGTCTAGGAACGACTGAGCGGCATCGTATAGACGATGTTTTCCGTGCAACATAGTACAGGAAC GGGCTAGAATTTAACTGTCTTAGACTATTTCTTGCCAGCTGGGGCCCTACGGGGTGTTCCCGACGTGGTTAATCAGAGTGTTAAGGGGGTATCCCTGCGA CAGGGCTAGTATGCGTAAATGAACCGGGTAGGTCTACCCGGCAACCAGCAGCCCCGCTGGCCCCTCCTACGTGTACACAGTGCTCGCTATACATTGACCG CAGTCGCCTGATCAGTCGCGCTAAATGTGCGGTCTACAGCGACTCGAAATCTCACGAATTACGAAGGATCAGATGGCTCCCGGCTCGCTGCAGGACGCAA CCGTCAAAGCCCTGTGGGAGTCGTCTAAGGGTCGGTGGGATGATAACAGTTATACCTCCTCATCATAGTACTTATGATGTCCCGGTGATCTCTTCCTACA CTTTGTACAGAGCGATCGTCAGGAAAAGTAAGGAACAGAAGGTTCCAGCAACTTTTCGACGTGATCTGAAGGTCAGGATCCTCGACGAGGGCGAATGCCT ATGGATATATGTTCTGTCTCTAAAGTGATTGATAGAGCTCTTAGGCCGCACGCCCGTTATGCATTTATTGAGGCCTGATGAGCACTGAAACGTTCTCGCG CGTATGAAGCGGCCGCATCAACGACTCACGTGGTGGAGAAGCCACTAGGTCTGGAGCGATGAATCGCAAACTAATAGCTACAGGGGTTTCCAAGAGGCTG ACCACGGTGGGGTCGTGTGCCATATCTGATCATCATGGGGACAGAGAGTACGTGGGAGAAACACGAACCAGATACCCGATTCGGAAGGAAGTGTTTCTTA CAAACCTCTCTTTCACCTTAAGTCGTTGATCACTACGTCCACTTTTAGAGCCTTGACCCGTTACCCACCCTACTAACAGTAGCAGAAGGATCAGGGGCCA CAGTAGGCGATAATTGCAGTCGCCTCTCAAGTTAATTCATCATTCATCTAGTCTTAGGTGCGACGACTCTAGCGAATATACACGCTTACCACGACTTAAT AATGGACAAGGCGGTCGAACATCCTCGGATGAGAGAGTAGCTGTTAGATGTAGTTGCGACGTTAGCACGCCTAGGGAGCAATGCCGCCGCCACTGTATCC AAGGTCCCAAACCAGCTAGCGTACGCAGACGTCCGTTAAAAGCAGTGTAAAGGCGCTCGTTGGAAATTACGCGCACTCTCCTTTGAGCACTTTATGCGTA GCCCGCGGATCTCGAACGATTGGTTTAGAACATTGGGTAGTAAGGATATCAAGGAGGGACCCTAGACTGATGCATCAACTCCAAACGCAACTTCTATACG AAGATGTTCTCACAAAATATAAATGCTAACCTCTCCCTCCGGCGAGGGGCGCCGTATTGAATATCCGAACTGCGCCACGTAGTCCGCTTAGTCAAGGATG AAGCCCAAGAGATCCAAGCGATCACCAAGGGCTGTTAGGTTCACGCCCGATGTTAGACTGGGTCGTCGGACACTTCGCCCCTCCGTGCTGCCCTATATCA CACCTGCGGACATCCATATTATGATCAACGGGACACGTTTCTCGGTTCTTCGGGACTGTTTGACATTAGAGCCCGAGTGTTCAACTAATCCTGAAGATGC AGTCGATTGAACATCGTTTTCTCGTGCATCAGTCGTTTGGTGTCACTGATGAGCTACCGCCGCGCCGGTAGACATCCCGGAAGGAAATTGGGAAAGAGCA GTGTTCTTCCGGAAATAATTAGACTCGAAAAACTCTTGTCTGATATAGTGTGTCTGTTGCATTATTTTGCGAGATCCCCGTTTCTACTGTAGCAGGGTGC AACGCAGCATTAGCGTCAGTCTTGATGTATTATTCATTGCGGCGGTAGGCGTTCACCATCTGCTTTAAGCTGCGTTAGCTGATTGTACTACTTGCTGCAT TATATGTGTGACCGTGTTTTTCGCTAGTACTCATTGGTCACTCGCCCCAACTCTCGGCTCTTACCGAGGGTATCCGGAAGTGCACAGTACGCGAACGAAC AGTCCTTATGCGCTCTGCCGTGCTGAGGCAGCAGACAAGACGCACGGGGTGTTTAATCCGCAGATTGGTCTGAAAGCGTTAAGCTTCTAAACAGTCTGTT TTTTTTGAACCTGGACGTGGAATCAGGAGCCGGATATAGTTTACACCAAAAGTTCTCCTAGGATACAGGTGCTCCTATTTATCCGTCGGCCGTGTATCCT GGTTCAGGGCATAAGCTATCTACCTGAACAGCCTAGAAGTGCTAGGCACGAGGGAGTGCCTGATAAACGAGCTCGCTGCAGCTTATAACAATCGACATCG TAAGCCACTGATCTTCTCGCTCTCATGAGTGGGCACGCTGTCGTAGCGTCCCGTACGACGCCTGGCTAACTGAATTCGGAGTTCCCGGCAGAATAGTCCT AACCGTATGTGAATTACCGGGAGGCGGGAGATCTCGTCATGCAGTAGCGCCCGGCGTCTTCGTACGGACTAGCCGCTGGAAGACACGTGACAACGAACCT GTGGAGGCCGGGCTTCAAATGTAAACATTTTCGCTGAACGCGCGTATATACCAAATAAGATACAGTGGCCGTGTGTAACACATCCTCTACATCAAGATGA AGAGATCGAAATGGGTTGAGGTGTCGCAATAAAATGCAAATAAAGTCGTAATTTGCTCGCCTATACAAGGCGGGATGCCGCAGTGACTGAGCCAGTTGAA GCGCCTGTAAGGGTCTGGGTGGGGTCGCAGGGGTCAAATTGAACGTTCACGTGGTTGGCACAGTCAGCAGTGCAGAAAAATATACTACCACGCGTTTGCC GCTCCACTGCCACGCGAAATTCATAATGTCGCGTTGCTTTTCGATAATCAGTGGTTTTCACCCGTCTTAGCTCTTTAATTGAACCCTTTTATACGGGACG TCAGACCGCAATAATACCGTCTGCACTTCTCTGATACTAGGACTCGACCATTCCTTTGCGAGTCTGAATTCTAAACATTCGGTCTTCCGGCATAGAACAT GTTCAAGATGAATCTGGTTCCCATTCCTCGATTGCGTCAACAGAAGTTGGCGCGATTTCCTGGACCGGCCCTAACGTTTGGTGACAGTGGAGCCTTCTCC ATGCACTGTAAACAGAATTCTTGGGGGCTCCACAAAGTACAAAAATCAGCCGTTAATCGAGACCAAACAGCTTCAAAGCATAAGTAGCGCTACCGTGTAG AATAGGAAACACAAGCAGCTATAGAGATTAGGCCGAATCGTACCCATTCGCATTGAATCTCGTTTCGTAGTTCGTTACCGAGAACCAGTCGTGAAGTGGA CCCCGCTGCTCGTACCTCGGGGTGCTCAAATCTGATAGGCTGCACGCCTTGGAGTTACCAACGAAAATATATAGGAGCTTATTTTTAATCGCAGAGAGGT TCAGATATAGCCCAACGCCCACAGGTTACCGCAGGTCTTATCGGTTAGAGTAATACATAAAGATATGCTCGTCGGTTGGATTTCTCCGTGGGAGAGATGT GCAGTGCCTCGGGGCTCCTCTATCCACATGGGAGCGGACTGGAGAAGGACGCCACTGCGCACTGTTCCTCTAGCCGCCTGTTGGGATATTGGGTCGGGCG ACCTAGACTCCGCTACCGATGGGACCGAACAATGTTGCCACCTGGATGGGTGGACTAGAATTTATCGGAAAGTTCGCAAACGCTTTTACGTTGGAAGTAA ATACGTGCAGGGTATAAGAATGCTAACGAAACCCTCACGACACTATAATACTATGTGCAGACTCTTCATAAGGTGTGGGAGACGTAATGGCGACTATGGT ATCCCTCGCAAAGCATTGAGCTGCGCGGAGGATGATTGATTACGATACCGAACCCAAATCCTGGATCTGATCCGCAGGTATACAGCCTTTCCAACGACCT AGAAATTTACGAATGCGGCGCGAAACGACTAATGGATAACGACTATGTCACTATGATTGAGATTACTGCTCCCTTGTATGTCGATTTGGATTTCCTTAGG CTTCCATGCGTATTGCACCCACGTCTAAGCTATCTTACGTTATAGGTTGGGGGACGGCTAGGTACACTAGCCACCTCTAGATCAGATTGCATCAAACCCG TGAGCACTCGTCCTCAACTTAAACCTGCCACTTCCCTTCCTCAACTGTACTCGTACTATTTCTGATTATAGCCAACCGGCTTTACCCTTTAGTCGGAATG CGTCTACGAAGGGGCACCCGATACACGGCCAGCCAAGACGATTTGCCCGGTCGGACTTCGTCCCACAAGTCCCTGACTTTTACCATCCATATCCTTCTGG TATACTTACCGCAAGAACCGAACAATTAAACATTAGTCGTAAGGCAGGGCTATTCACTGTGCCGCCGCGGGCGATGGGCTCCCCTCTACCTACGTCGCAC AGAGTCGTTGTTAGGGGAGAAACATTCATGGAGGGTCCGTGTACCCGCTCTCGAAAATGAGGAGAGTGGTGAGCCCCACATTTCTTGAGTGGTGTAGCTT ATTTTACCGTTAACGCGTCTTATGTCGTCTTACAAATCATCTTACGTGGTGGGAAGGCTGGGCCGTGGACTGTCCCTGCACAGTGTATTGCACGACGAAA CTAAACTACCGTATAACCATTAACGAATATCTCGGCCAGCATATGCGCATCATCGCATTTAAGAGACTCCATTCCGCTGTAAGTAACGAGATACTAAAGG TGGTTGCACCACAATTAGTGTGGATGACGCGCTGCGCGTTAGGGTTTGTGATTTAGCGTGACTGTAGCAGATTTTAGGATCCGCGCCTAGTTGGGGAACT GAGAATCTCTGAGAATAATTGTGTGTCGTCATCTTCAGTTGACCTGCCTTCAGTGCGTAGATGTGTGTTTCTTCTTGGGGAGCGCCTTTGTATTCCTTGA ACAGTAGACCCGCCGCGCCGTTTATTGGCCGTATTTTCATCCTGGTTCTTCCTTACGGGGGAAGGCTGACGTCCTGGATTTCGGACGACATTCACACGCG ACGCCGTTCCTTAAGTTTAGTACGATAGTCGAGATCGATCAACAGGCCTGAGTGAAGTTTGCGGAGGCAGATAGAAGGCCCTACTGATCATTTTTTTTAC CTTGCGCTGAGTTCGCGTACTTCCTCCTGAAACTGTCTCAAACGCACGATGCCTCATCTTCACGAGTTCGAACTACTCAGACCACTTTGCGGAACGCCCT ACCTCCGTGCATCCGAATATATGAGAGAGTACGTAGGAACGAGTCCAGTGGTGCTCACGCCGACGGGCTAATGTGACGGCCCTGGGCTCTATAGGTATAC CCAAGCCACAGGAATATGGGCGGTATGGGCAGCCATAGGAAGGGAAGCCGATGGGCTCCTATCGTAATATTACACGGCATGAGCTACCATGGGGATGTGT GGTCTCACCTAGCTGGACCTCACTTTTAGCGTCTTCCCTGCCTTTCGCGCCGGGCTCATACGCTTGGGGTTTATCTCGACGGGCAAACAGATTCTCGCCG AGGGGATGTTCCACGTTCGGAGCTCCCAAAGCTAGCCCCGGAGTCACGTGCCACACACGACGTTTGCCATTGGACCTCCCCCTCCACGAAGCGACCAGCC CACCGGTTCTTACGATCTAGGTTTCGGAGTTGAGGGTAAACGACGCAACCTGTTAGTGATGAGCTGAGGATCTTAATCTGCCAAAAGGAAGCGTTGGCTT GTAGAATCTTAACGACCCCTCCACATTTTATGGGCAAGGATATTGGTGTAGGTACAATATTGCCTAAGGTAACCTATGGCAGATTTGAGATCTCAGGAGT CATATTGTCCTGGATACATCAAATCATTTACCCCGTAGCGGCGGTAATCTTGCCAAGGTTGGCGCCGCTCCTCACAATAGTGAGTTTTCTGCCCGTTTTT ATTGCGCCGAGATGTCCTATACTGGATTTACATCAAGGAGCGCAGCTTATTGAGGGCGTGTTAGGAACTTCAAATGTCAGGCAATGGGCATCTTGCTCGA TTTTGGCGCGGGCGGCATTTATTTTAGCATGTATACCGCTTGGGGAGTAGCACTTCGTCATGCTTACCGGGTTTTGACTCGTATACTAGTTTTCTATGTC TACCCGAGGCCATCATATGGAGAGTTAAGATGATCCCTCGAATGGAGAGAGCGGTAAGATGTGGACTGTCCTGGGAAGTTCGTCTTGAGCAAGTTATTGG GGAGGCCCAGAGCGACTTCGATTTACGGCTATCCTCCGGTATTGTTGAGTTTTAAGGGCTAACAGGCCGGCCTATATCTTCCCGGCGGACATGTGGGCTG ATCAGTAGGCGATCATACTCTCCACACGTATTGCCCGCCGCATGACTTTTCAATCCCTCGGCTGCACCTACATTATCTTAATATTTTTCCAACACTGTCA GAGGCTTCTGACGATAGCCGTACGCAGGTGATCGCTCTACGCACGGCCCTGAGATGTGTTACCTGAAAGCACCTTTCGTGCCGAGTCTACTAATGCACGA TCTTTGTCAATTGGCTTACAGCGGCTGGAACGGGCATATATACCTTTGACGGAACGGCTATCACGGCCCACGACCCTCACCTACGGAGCTAGCAAACACT TTATCGGAGTCAGTATCTCGGGGTTCCATACGCACACTAGCTTGCAGTGCTTCGTACCAAATCGACCATATACCCAGACCTAAACTAAAGGGGGCTCGAT ACGTTAGTATTCTGTGTAATCCATACAAGTTCCAAGGGACACCCCTGTGAAGGCAGCCAGGAAGTCGCTCGCCTACGAAGTTGCTATTGTTGCACCCTCG CCGAGCACAGCCTACGGTAGTTTTACATCCACTTATGCGACGGAGACGAGTATACAGGAGTGCTGTCCCTTCATCGTCCCTCTTAGCATCGTGGGTGGTG CCTCGCAGTGGTCCCGTCCAAATAGCGGCCCAGTCGGGCCATTAGAGACACTCCTTTTCCGACCGGCGAGTGTGCGCCATTTATATCCTACTGGGCTGAA CTTTCAGCTCTTCGAGGAGTTGGAGCTAAGACTCAGCAACCTATACGTCCCTTATTTAGTCTACTGCGCGCAATGCACCCAAACCTGATCTCTTCACATT AATGTTTCGGACTCACGTTTTGTCTACCTAGTTGAGTCGAAGCAGTACTTGCGGCGATCCCAAATCACGAGAAACTCGGATTAATTGGGGCTCGTGACGA TTTTCTTACTTAAGACAACATCGCGGTCAATTCTTTTGGATCTAACTACCAGCCTTGTCCAAGATAAACACGGGGTTGTCTCAACGGGTCACAGCAGCCC CATTCGAAAACGCGGACATTTGTTAGGCACCCGTTCACGAGTAACCGCTATGTCGTGCCAGAGCTTCCTACCACTGCGCGCACAGACCTTGCCACGGAAC CTACAGTAAACCCAAACTGATCCGTGACTGCATGCTCATTGGTTCTTAGTGCTATCAGGTGGAGCGTCCCTGCGCAGACTAGAGCTAGGTGATTTTGTCT AATATATGAACCCGTACCATCGCTCTACCGTAACCTGTGCGTGGACCCTTCGAGTCCCACGGGTCATCCGACCCCCCCGACGATGACATGCCCTAATACT ATGCCCAGGATCCCAGTACGCCATGATATCGCCCTACGTCCCGCAACACAGAAGCCTTGGTCTAACTTTCCAAAAATTCGTGACCATACGCACATATGCA TTAAGCGTTACGCTCGTGTCCAAGAGGGGGACACTCCGGTCCGTGCTCCTCCCTATGTCGTTGCCAGCTCAATGCGAAGCAAAGGGTTCGTCATGAGAGA TTCACGCGCATTAAGAGGGGCTTAGCTCTAACAGGAATCTGACCATCTACGTCTGGCAGATCTTAGAACAATGCACAACGATACGTGGTGGCCGGGGCGA AGCCTTCGGATACATTTCAATGACGAACCCGGACAAATCAGAGACCTGCTGATGGGGAAGTTTAGTCGGCACAGACTTGGGAAATATAGCAACGCTGTAA CCTCTGATGTGTTCCCAGGGTGATGTATTCTCTCTGTAGTGTAGTCCATCTTCGACCTGCTATATATTACGATTTGTGTCCAAACACCCCTTTAGATGTT CGCGCCGAGGTGACTCGTACGGGGATTGTGTCTGGTGACCGTGCTTGATCGGTGGCACGTGCATATGGAGCTTACGTGTATTTGATGTCCTAGTTGGGCC CCTTCATATGGTATCGCAGCGAGACCAGACAAAAAACCAACGCCTTAACTCACAGATCCCGCGATTCGGCACCGGTCCGATGAACGGTAGCATCTTATGG CTCGTCGCAGGCCTGCTACAATTTCGATGATGGCCTAGCGCTTTTCGTTGAAACTATGATCCGATGTATGATGAATTTGGTTGAATAGGTGTCGCGGGTT AGCCGGACTGTCCATTCTTCTGCGAACACAGGACTTGACCAACCTGTGCGCTTATATGGGATTGAAGATACGTGCATCAGTTATGGAATAAAGTGCGATC GGGACGTATACATCGGACTCTAACAAGAAATTACTATCGGTACGCTGGGCACCTATTCCGTCCACCAACGAATTGGGCTCTGCTAGGTAGCGTACCCCCG CACTGCGTCCAGTCACAAGATATGTAGATCACAATCTCTTGTTAGACTATGAAGATTTATGTGTCTAGAAAAGCAGTTTGCTATACTACGGCGTCCCATT GTCATGCGACCAACCCTCCTCAGCTACGGCGGGAGGCCACCAAACGTTTTAAGACATGCGGACGGCAGGTCTCCAGTGGTCTCCATTGATGGCGCAAGAA CAAGTCTAGCATAGACGCGACGTGTGCATCTGAGGTGTTAGTGTCCCCTGTTGGCGTGCGGAGACAACTATAGCAATGCTCTGGAAAAAATTCTTACCAT CTCACGTTCCCAGTGGGACATCGCGGGCATTGACTTTACGACCCGAACCGTGTGTCGGAGCGAGATAAGGACCACCGGTTCTCGCGCTATCTCGCGGGGC GAGTGCACTTATGTGGAGTCTGGATGTATGCTCGCGCATATTTGTTTAAATCCGGCTAGCGGGTTACATGTAGCCATTTAGAAGGAAAGACCTCCGTGCG ACGCTGCCCTACTCCTCACCGTGAGTCCAAATAATTCACTTTGACTCGTCACGATGCATAGCGTCCCAGCCCTGAACTCTAGGACTTTGAGACAAATTCG ATCACACGAGCCTTCCAGAAGAGCGGACATAAAAACTACGTCACGCTATCGGGTCGCCCTCATTGTCGGACGAGACCCTCTGCGATTGACTAGTGTATGG TTTGATCTACGCTGTTCGAGTTGGGGGTACAAAGCAAGTTGTCGACAGCTCCGAACTCGAACTCCGCCTCACTAATGATAAGTGACGAGCCTTCTGCGCG CAGACCGGAATAGGGATCCTTGGGTAAACGAGACGATCGTGCGCGCCTGCGAACGTTCTTACATGACCCCACTCACAGTAATCTCTGAATCTCGCGGGGT GGGATGCGTTTCGTGCCCGCTGTTTGCTTAGGCATGAGATCCCGCGCCGTCGTTCGCGCCTCGAAGACATATCGATGCGCATAGGGCGACTCTGCGGGCC AATTTGCGGGGTGCACGTCTCGCCAGGACTGCCGCTAATAGTAGCCCATACTGCAGGAGGCAACTTATGGTGAGAAGAATCACTCCTATTCTTAAACGAC AAACGCACGTAAATTGGTTCCACAGGACCAAGTGACGGGACCACCTTTTCCTATCATAGCCTCCAGGGCCCAACGGGCGTCTGGCTATTTCAGGCACGTC ACACCCCACCTACTCTCGGATGCGAACTCTATTATGAAATAGTCGCTCGGATGTTTGTATAGATGCCAACTGTTGATCTCGTCCGGCCAGATCGATGGAA CCTGACCCTGCGTCGGGAGCTAGATTTTATTACTAGAAATCGGGGCCACTACTGGAACATTGACAGAGTTTATGGACTCTGTAAGCTTAGACAAGCCATG GACAAAAACCGAGCAGTTATGACCGACGTGCCGGGTCTTAGGACTCATCAGGATATCTACATTCGCAGGCTGGTGAGCAATAGCTAATATTGAAGGTGGC ATAACGACAACGATGCCTGTCATGGCTCTAGTGAGGTTAGCCCTGATGGACTTCGCAAGCATTATATTTCAGACGCGGATGCTCAATTGACAAAAATTCG GCCTCGACGCTAAAAACCCACCATCGGTCAAGCTCGAAGTCGAAGATATTTCTGACGGTCCGTACGAACATGTACGGTGGGAAACACGGTCGTACGACGT TAGCGGTAATGGTAAACGGGACAGGTTGATCGGTCAGGCGCTTTACACATTACACATAATAGTCTTCCCCTAGGATATATGGTGATTGCGGCTATCTCGT CTGTGCTTGCTCCACGCCCATGGTATGGCCTCGTTATTCGGATACCAGACTGGGCGCTTTGGCCGTCTTACGCTTATCGGGCGTTCCGTGGTTTCCGCGG GCGAGTCCGTCTTCCCACGAGGATCACTCAATATGTAACTGGCCACTGTATCGGAAGCAAGTTGGTTTTTAACGCCGGAGCAGCTAACCCTACGGAGTCT AATCATCACGACTGGATTTAGTACCTCGGTAGCTGTCCTGTTATCGACTACAACTTCTGGGGGCAAGCACACTGTCATGCATCCATGCTCGGTTCTCCTA CTACGTTTGGTAGTCGGGGCCAACAGCGCGGACATCCGAACGTCGTCTGAGCATTTTGCGCTTACAGATGAACGGTACAGCAGGTGGGAGTAGGATATCG TTGACCATCTGCATAGCGCGCCACAAGCTGCTGCAGCATGAAGTACAGTGCCGTCGCTGGGGGCCGCGGTATACGTCCGTTCTACCATAGATAGCCTTCT TCGATTACATACTTGGAGGGGGTCACGTCACTAGTCATGGAGCCGGCCCGGAACGACGTGCAGTAACTATATAGATGCGGGGTGCAACGACACTTCCCAG CTATGGAGCATTGTGACGTTGGTACAGGTCATAAATAGTAGCATATATCAGTGTTAATCCCTATCAAAGGATTTTGCCGTGAGGGAAGTGCAGATAAACC GAAGAGGACATATACACAGTCATATTCGCATAGTTTACGCGGTAAGTCATAAGAGCTCCGATCACACACCAGTGTACGCACCCCGATCATACTCATGGCC ACCTAACTGTGGGTTTGGGTCAAAGTCGTACGAGTTGCCCAGGATAGGGAATCACGTACGGTGTGGCGTATTTTTTAGTCGCAACGTCACGTGCACAACA GTCATGAATAAGCCAATCCGGTCACCGTATTTACGTACTGTAAGTAGCAGAATATAACGGCAAACTTCCGTTGCATTGCACGTCAATACGCAGAGACCTT GCTAATGCGAGGTAACACCAGTTTACGGCCTACCACCTTACGATCCATGATACTCACCGCCATCCCGTTTTTAGTAAATGATCCTCTAAATGGTCTACAT GAGATGAACCACATCCTCAAAATAATGGTAGGCATTCATCTAACTCTTCGTGTCAATAGAAGCCGGTTTCCGGACTGCTACTGGGGCAGTTATTGATGAA GAGGGAATCGCCATCAGTATTTTACTGAGGAGCCCACGGGTACATATGTAGGTATGGCCCCTCTTTACGCGCTCAGTTTGGACTCGTCGGTGTTTAATCG GGTGCGAGCACCCATGTTTTTTATATCCGCGCAAGACCATAGGCTGACTTCGGTACACACACGCCTCGGTGAGCGGGTTGGACTAAATGAAGTCATGGGA TAGTTATACACTGCGAGTCGCCGGCCCCGAGCATTTATATGCACTATGTCTCAATCCATCGCATCCGCCTCAATGATATCTGACAAATCTGGCCATTTAT GGTGGAGTAGCGGACTTACTAGCGATGCGTAAATTGGTTGTTCCGAATCCAGCATCAGACTGACTCAAGAGGCTAAGCGAACAAGTATCTCCAGTATGCA GCCTATGCCATTGTCGGCAATTCTGTCGCAGTGATTAGTTCACAATATGTCCCTTCTACCATGGTCCTCGGCCGTAACGATCATCCGCTCATGACCCCAA TCGGAGTGTTATACGTTATGAAGAAAAAACACGGCCCTTTCAAGGCCAGGTGACGAGATCAGTAATAACCTCTGCTCTTTCCCAACACAACCGGGTCATG TATACTATGTCGTGGAGTAGTCTCCGCGGTCGCATTTATGCATCGATGTTTCCTTTTTACGAGATTGAAAATAAAAGTGTCTTAGTACGTGGACTACGAA GGAGTCAGGCAATACTCGTCTCGCACAGACAAGGATCACCGTGAAAGAGCGAGGATAACCCTCTTATGGGATTAGAGTAAAGTCTTCCACCGAACACGCG CAGGGATCTACAATCATATTACACCGTCCTTCCTTTAGAATCCCTGGGGAACCGCCGACAGTGCATGAATGTGACCACACTGGTAAGCTTATTATGAAAA CACACTTAGGTTTCCAATCTCAGGAAACGACGAACAAACTCTGCACACAGAGTCCGAGTAGTATTAGCCGCATAGAAGGCAGGGAGGTTCGTGCCGTGTA ACGAGGACGTAAAACACGTTGCTACAATAGCTGGGGGCAGCGGATGGACGAAAATACGCTTCTCACGCAATTTTTCTGGTGGAATGATGATGTTGTCTGA CTAGGTCAACACAATTAATCATAACGCGTCGGACAGTCCAACGACTGGCGCTGTTATTATCGGACTGGAGTGCCTATGCTCAACCGCGGACCGACGCTAG CAACCCCCATTAACGTACTTTCCGCCCTGGTAACTACCGGGGTCGTTCGCGCGGGTTGCACCTTAATTGCACCTTGGTTTCTATGCTAGGACTTTGAGGC CGATAACCCTACACTGACCTCAGTGAAGAGAACGCCCTGCTTATATCTCTTTCAGGTCTGATTAGGACCGTAGTCCTCCAGACTGCATATGGTTTGGTGA CTGATGAATTGGGATGGGAATGAGCTCGGAGAAAGTTAACATAGGGGGTATAGTTCTATCCCGGTTTTTTTCATGAATTTGCCAATGATCCAATCTGGTG GACGGAATACGCCACTCTACTCAAGTGTAATCCCCGAGAGGCTCATTGACCGATCCCTCTTAGTCCTAGGTATCTTACCCGAGTATTCCTCCGATTCGAA CAGCTTGCACGTGTGGGCACACATGAACTGTATGTAAACAGCCTCTTTACGGATACGTAGCCAGGGTCACGGCTTAACGGCATGAAAATGAATGGCCCCT CTGGCTACTATTTACCGTTAGACGCGGACGGTAGAAAATTATGGCTTCCTGTACCCGGATCCAACGTGTCCTCCCCTATCAACGGAGTCCTATTCTTTTT AGACGTATGGTACAAGGTCATCTTTATAGCCGGCACAGGTCACAAACCCACAACAAGGGCTAGTACGCTGCTTGAGCCTGCGTGTGGGAAACTATACGAA TGAGTAAACGTGCTACACTATGGCAAAGAATGAACAAACAACTCGAAATTGTAGGGCCACGATTTAACGGAACGAGGTCGGTACGCACGAAGTCGGGAAA ACGTCCATCAAGGGCACTCCAATCTCAAACCATCTATCCACACTTGCAAGGTCGTGGATGCATGTCTTATCCTAATCGTGTTGTCTAACGGGTCGGCAAC AGGGGGTTGTCCGGCCCATACACCGCCTCCGAAGTTTCTCGTCGGGACGTGGGCACGCGGGATAATTGTCGACCGGAGAACGAGTAAGCGTAAGTCGCCA GATTGAATAACCGTACAGGTTAGGCAAAGTGAAAACTAGAGCCCAATAAGGCACAGCTGCCCATAGCATTTGCTCGGGCCTTGACCGTCCAATCTTGTCT AGCCAAGCGTGTTCTGAGCAAAACTTGCGGGTGGCAACTGGAGATACAGATCCTGATTTTTGAAATCGAATCTCTCCGTGCGTCAGCTGTAAAGTCTCGC GGTTCCGCCCCTGTATGATCCTGAAGCGCGCACAGATAAGTTCTCGCGTGTCGCACAGGTACGTCGACAGGATGGGGGTCTTAATTATATACACTGACGA GCTCATCCAGCAGCCAAATTTTGGGCGGTGTAGCAACCCCTGCCCGATGAACAACAGTGGTCATTTTCTCACCTTTGCTGGGGTGGTATAAAGTGGTTCT CCTTCATTAAATATCAGGCTAGCGGGATACACGTTACATTATGAAAACTGACCTTTACCGCCGCCTACATCTACTGGTCCTGTTACTGTACTTATCAACT CAACTAGTAGAGTTCGTCTACAAGGCTAGCGGCAGGCCAATGCCACTCGACACCTCCTGTACCTTCATTTGCCGACTGAGCTGAGCGCAACATCTCGATA ATATTCAAGATAGAATAAAGGGCGCCTAGGTAACTATAATCGGTCCGGACCAAGGAATGGCGCTATCTACATCATTTCGCCTTCAGAGAGGGCATCAACA AGGCTACTAATTTTCGTTTCAGGGGCTACTCACATGCAATACATGGAGAGCGAAACAAGTTCGAACAATTTACCCGGCCTAGATCCCCCTAGATCGCCAA GCCTGACACAGGTTTCGTCGTCACACCGTCGGTTTTGCAGACACGGGGTTGACCTTCTCGGGGGGTTGGTATAAATAGGAGGCCGCAGTCTTGATCCGAC CTCCGAGCAAAGCACCACGCGGGCGGCCTCTAGATGAGTATTGCGAATGATAATAGGGCTTTCGTTCGGCTAGTTGTGGCTAACCAACCACAGAATTGAT CGGTACCGGCGGGCCGCTCGTAGCTCGGATTCAGCCTATGAGGGCGGGTGAATTGTCAGCATTTCTACTTGAAGGTCCGCCGATAGGTCAATTAGTTCGC TGCGACGTATTTCCCAAGACAGTCTGAACACGTTGGTAATGGCCTAGAATTGCACCCGGGATAAGTGCGAAGTACGCTCCCTTTAGTGATAATACTCCGG CCTATCCAACAGGGCGTCTGTAGATGAGGTTCTAGGGGCACAATCATTTTCCACGAACGCATGGTCACGGGATCATGTAACACCCTCTTCCTTCCAATGA GCGAACTGCTTTACGATCTAGGCGGGGCGACAGTCCGTGGGAATTATTGTTTAGTATCGAGGTTTTCGTAGGACGTAAAGCACCATCTGTAGTAGGTAAC GCGTACATCGGGAAATCGTTACGAGAAAAGCCGAGGCCCAAATGATCTATACAGACTTAAACTGCGCCCCAAGACTTGACTGTTACGTTGCGGAAAACGA ACGTTTAGCCGCCGCCCAACACTAACATTGGTAACGCTCCTTGACCTGTACTCCGCCTCTCAGTTGAGGCACGGGTAATTGTACCGCTATGGCGTCTCCG GGGAATGTCATGTTCAGTGCAGTACAGCTAGGGGCTGCAGAGCATTACAGGGATCGGGTTATAGGTTGCGTACGAAAGTTGCGAGTTAATGAACGTAATG AATCGCATAGCTAAAAGGTCAGGAGATCCTTAGTGCATTCCGACCGTAAGCTAAGAGGAGGAGGTCACGTGGCTCTGACAGTGCTTCTGGCCCCGATCAT ACAGCGAAAAGTATAAGTTATCTAGGTGACCATAGCGACCAGACTGAACTTTTGTTGCGATCTGGATACCCCCTATCCGCAAACAGTCATCTCACGCGGT GCCGGTCGGCAAGGGTACCGTACGGCGCAATGGAACTTGCGGCCCGGCCACGGTCGGGAGGCACTACATTCCCATACGGTGTGAAGGTTTTATAGTTTTT ACTCAAGGCTACAAACGTAAGCTCAATCTCGTCCGTTCGGCCTATGAAACGGGGTCCATATCATGGTACAGCAGATCAAGTTGTACTTATACCCGCTTAG TAACGTATTACGTTGGGTCTCAAACTTAGTATTAACAACGATAGAACGACAGCGAGCGTCGTGTTAACTATGGACCAAGGAGAAAGGGATTGATCAAGCC TAACAAGCGTCGCTTGACCGACTTGAGGTGCGATCGTGCTATCAGCTACTTACGGAGAGACAGAGTTATCGCAACCCAGCATCGACGGACGTCCAGGCTG ACTTCCGTATAGACTTCATGGTGTGCTTTTCCGGCTCCTTAAGTCGTAGTCGTGCCATTTCGGTGCTTCATATTTAGTGTGAGCCCCCGAACCATACGCG AAGGTCCACAGGGAGAGGACGATGTGCTACAGAATGCTGAATTTGGCTGACTTAGGCCAGAATAATGGATTAAATTCTGGGAAAGTCAGGTCAAACGACC CATATCGTCGACTTGCCCAATCGAACTTCGCTTAGTAAGTAGAAACGGCGTTCATGCAAACATTTTTAATATTGGGAGTACGCCGGGTGGGGCTTAGTCC TTAAGGCGCGATAGGAATACAACATTAACTTTTTTAATGGAGTGTACGGCAGACTAGGCTACAACATATGTTACGTGCGAGTGACTTGAGAGTTGCGCTT AATGAATGGTACGGCAACATCAGCACACAATCACTCTTTTTCTAGTGCTCGGATGAGCGTGGCAAGCTGTTCATGGTGGTTGAATGGGGCGATCGCCACC TACCGGTAATTGGCGGGTATCGGATACATCCCAGGGCCCTTCAGGGCTGCTCAGTCGCGTGCTGCGGCTCTCTTCACATGGGGCTTCCCTATTATCTTCA TGGAGCCTGGATATGAAGAGCGCGTGTGCGCTGTGCGGCCCCATAACTTCGTGGGCGGCGTAGCGAGCTCACCCCTGCCGCCATGAGGGTTCCACACCAT TCCTAATCTGAGCACTCGTTGCGCTTAAGCTGCTAGGTACCAAGGGTTGCTGTAAAGACCCCAACAGCGCGCAAACCACACGATGTCACGCGGGCAAAGT GGTTGGAGGTTAGCAGGTCACCTGGGAATATAGTCAGAGGGATCCGGACAAAATGAACCATCGGTCAAGAAGAAGTGCTTGGGCGATCGGAGGCGCTGCC TAATTACTGCTGGTACCGCGCCCGTGGGAAATGCAGTAAGCTTCATGACCACCCGGTCCAAGCGGTAATGGTAGTTCAGAAAGAACACCTCGTACATTTT CCATTAGATGCGCGCTGGCCTTTTTCTGCCTTAGCGGCGGCTGATTCTGGTAGACTTTCGGGAAGACTGCTTGTTTATGAATTGGAAAAGATAGGGAGGA TCTATCGTCCCTGGGCCTGATGTCCTTCCCTTAGCGGAGGATTTTAGTCCCAATGTCTTTTGTTAACTCGCGTCAAGCCAAAGATGTTAACCCAGGCGCT TCCCAACCCTCAGCCCTACGCGTAGTACACGACAGATAGCCGCTTCATCTTATCCCGTGGACACGTAAGTCCAGGGGGATCATGCGGTTCCCTAGAGTGG ATAGGTAGGCTTTATTGCTGTCTTGTGGCCCGCTCTTTATGGTTGTCGATCACCACGAGTTTTTTACTACGGTAACATGTCTACGTTTGAGGTGGTAGCA GCATGCGCGACGTCAGGGTGGCGACTAACGGCAGATAATTCATCGCAAAAGCCTTGCCGCGTGAGTTTGTCTTAGGTGAAGCGCAGCTCACTTGAAGTTA CTGATTTACCTTACGTTTACGTAAAAAAGTCAGTTAACTTCTCATTGTTGTCGCAATGAGCAAGAGATCTGCTCACTGCTAATAACATCTTCGCTGTAGT TCCTATGGTGTCGAGTTTTTTTCACGCATAATGAACGTGAACACTTCACTTTATCTGAGATGTGAAAGGATGGACCCCACAGCGGTCGCGCGTCCGCGCC CTCTGACTCACCCCCGACCAGCTACCGGGCCAGCCTCTTCGGTAGTTACGTCGACACTTAAAACATGTATGTAGCATCCTCGCCTGATTCGTTGGGCCTC GATCTTGAGTCGGCTTTGAAGCAATTTTTTGTTATGGCCAAAGGCGTTTTGGGGTTGCGCAGTTATGTCATGGCCCGCGACAGTCGTTGAAGCCATATCT AGTGGTATCCTTAATACGTTTAGTATGGGGTATAATACAAATTCAGTGATAACTTCGAGTAGCTGCTTCCGACTGCGTTCGCGCACAAGAAGATCACAGA GCTTCGCTCGTTACCGGGGCCACTAGTGGCAAGGATTCGTAAGGCACAAGCCCGCTCTGACAAAACGACATCCCCGCAACCTCAGTCCGCAAGGCGCCCG GGTTTCAGGATCTGAAGACAAGATGTCTATCAGATAACCTAGGGATAACTCCCTGCTTTGCACAGGCTCCCAGTTCACCAAGCAAAGCATCAGTATCCGC CCAGGTATTTACCGGCGGTCGATGGCACCTAATTCATTGACAGCGCTCGTTGGGAGGGTGGACTTACTCCATCGAAGTTTTGACCTGGTTGGGAGTTCCT GGACTTCCCGACACTCCTTACGTACCGACTAGGTGACGTTCAATGATGCTCCGCGATCCTTCATGGGGCGCTTCCTAACCAACTTACTTTTTAGTTTTCT TCTAAGAGGATTTTTAGTTAATACTGCTAAACGTCTGGTACTGATGACATTGTGCGCAGGGGAGGATGCTCTCGTCCCCCGGCGTTCTACCAGTAGTGTA CGACACTGCTCTATATTGGACTTTTGTTTCGCGGGGATTCGCCTAAATAAGGGCTACTATTGGATCCGGACATGTACCCAGATCAGACCCCGCGGCGAGA GGCATGTCCGAGGTCCCCTCCACGCGGTTCACGGTAACCGGTGTCGCTCCGCTGTCAATTGAAGGTGGTAATAAGCGCACCTCTGATAAAACCGCCTCAT ACGATTATTCACTACTACCGTACCTTCCGAGATTACATTAGTGTTCCTGTGAGCTTTTTAGCAGCTGGGTGCGCGCCGCAGAGTCTATATCGGTGTCCCT CCAACTCTGACGACCCAGCTAACCGATCTGACGGTTGCCCGAATCACAACTCCAAATGCTCAGCTCTTAGACACAGAGTGGAAATCGATCTCCCGTAACT CGTGCTGACGAAAGAGAAAAAATGCTACTCTGGAAACGACAGGTACATGCGTCGGCAGACCAAATCGCTAAGCCCCGACTTTGAAGTTACTGTCCGCTAT AGTATCATAGTAGCAGAAAGAGAGTAATTGTGGCCATACACGTACTCATACTAGTAATTGTGAGTTATAGAACCAGCCGATCGCAGACGTGTGCAAAAAT TGCACAATGAACGTCGTGTACGACCATCTCGCATATGTTCCAAGGTGTAGAAGCCTCCTTTAACGACACTGGAAGCCGACCCCACCGCTGACTCGAAGGT CCTTACTGAACGATCCTACCGAATTTGAATGTACCGCGCTCTAGAGGGGGGATTCAAACTGAAGAAAGCCTGTCTCTCGCTTTAGCTACACTGTAGCACT TCGATTGAGGGTTTCAATGGTCAGTCACTTCGCTGGTCTTCGGCGCCAAGACTAGACTTTACTATAGCCCGTGCAACGCCGATGTCATCGCCGCACGTAT CTCGGACCATGCAATGGAAAGTTATATCCACAGACGACAAGAGAGGAGTGTTGATCGATAATGGGATCCGCCGGCGGTGGTTGCCCATTTGGATGTCGAG TCGAGAGAATCACATATACACATTCGGTCCGTACCCGCAGACACATCCAAATAAGGCCCTCCTAGTAGCTTGGAGGATAATCTCTACATTGTGCGTGAGC TTTTTCTCGGTTATCATGATGGGGGGCTAGCTGGAGCGGAGGGCGTCTCGGACGAGAGGTGAGGCATGAGTACGCCGGATACTAGAGGGCAGATTGACAA GAACACGCGGTAAACTGGTGACCGTGAAGCAGCGCTTTAGGACGGTAAACCCGTCGAGCTACAAGGTCTAGAAAACTACATCGTCAGCCCCGGGTGCTGT CGGAGGTGCATAGTGGCGCACGCGGGCTGACGCGCATCTAAAGTCCGATGATGGAAATCCTCCTGATGTACATTCGGTGTTTGGCAGCCCTCGGGTGCCC CGCTGCCACCGCGCCCGCTCCCCGCAAAACCCGAGGTTTTGGGAAAAGCAGTTAGATGGCTCCATAAAAGTGACTCGGTCCCCTTCAATGGCACTTGTTC GTAGTACCTACAGATGGTGCAGAGAGACGTGCCTGCATTGATCTCATGCTTGGTGACCCAGCGTGTCGGCACGATACGCATAATATCCGCTGCTAGTTAA GGTCATTTGACATGACCCTGCATTTACCTACGCTAGCCGGAACGTCGGTGATCGCTCTTCGCACAATCGCCTCATAAGTTGTAGACCTATCCAAGAGTGA GTTACGAGTTGGCTAACAGACTATTCCTCTGGGGGTATTCCCTGCTCTATAATGTCATTAGATGCTCTGAATGTACTGGTCTGACCGCCGACAGATAATT TAGCCTTTACCTGGTCTCTCTGATAGCAACGAATCCTCTCTATGAACGGCTATTTCAGTACGGTGAAACCGCTATATAAGGGTTAGTGGGCTTCTCGATG CAGCTATAAGGTTAGAGATTCCGGGATTGTCCGGAACGGTGGCCAATCCTTTGGTAATCACGTACGCCATTATAAAATAACCCCTCTCAGGCGGGCGTCA ACCTTCCCCAATATATTAGTTTATGTTTGGGAAGCTGATGTGATATACTGGGGTACGATCGTTCTCGAAACTGTTAATGTTGCCACACTCCGGCCACAAC CGATTGTTTTGGAGGAACCTATGACGGAGGGGGTGTAGGGGATCCACGCCCTTGAGGACAGACCACTGGACAGAATACATACCTGTACTTGAGCACCAAA GATGGTATAACAAACCGCCTATCGGGGCCTCTAAGCATTTTTACTTGAATTCGAGCATCCAGAGACGTTGTTATCTCAGGATTTAAATGCTTTTCCTCGA TGTGCACCTCTAAACGAGGGCCGTAAACGCAGAGTCGTCATGTAAATTGGTGTGCGAGCGAGGATCAGAAATACGTCCTATACCTCGCCCGACAGCCGCG CACTGGATGATACACAGTCCTAAAATGTGAGTCTCACGCCCGGAATATGGCCCGAAAAATGCGGTCGGGCTCTAGGTAAGGAGCATGATTTAATCTCTAG TTAGTATGAACCAGGAGGGGCACCCCCAGGTCGATAGGGTGGGGTCGGTGGGGTTCCAGAGGGATGTTTCGCACGGACTTTGGTGCGAAAGGCCGTTGAT GGCCCCGCGACTTCATTGACCTTTCCTCTCAATCAACAATGTAATTTTACGAACGTGTTCCTCACGACTCATCCCCGATCTAGGGTCTACTAGTTCCCCA ATCCCATGGCGGACTGCCAGGGAAATGACCCCATGGAATTATCCTCCGGTCCTCGCCCCCATCAGATCATGCTGAACTAAGGACGATACTCCAAGGTTAG GCGAAGGCCTGGTCGCCCTCACAGCGTGTACGATTTCTTACGTCGCTTTGCTGTAAGTGGTCTCGTGAGTAGTCGTCTATTGTTCCTCTTGCCTACCCTG GTTGTACTCGTAGGTGGGCCTCTCACGCACCGAATAAAAATTATTCGTGTGTACCAATTGTGATTAGGAGGCAAGGTAGTATAGGGTCGACAATTTCGGT GAGTATTACCAACGTATATCTGCGACGATTAGAAACCAAATGTACCCCATGACGTGCGAATCATGTACGCGCGTCTGCCAGGGATATAGTCAACGGGACT AAGAGTTGATCTCGGCAGTGTTGCAGTTTCCTTCGGACTACCACGGGTAGTGAGTGATCTAACTGCCGTTAAATCTCATAGAGGTTCAATTGGCTGCCCA CTACCTATAGATCTAAGTTTCCGACGTCTTGAAGGGTTAGGTTACCCCGCAAGAAGGCTGAATGACATTGTCCTTACCCGACCAGTATGGGCTGGCCAGT GATCGCGCTATGGCTTGTCCGAAGACCGCTACGCTACCACCGAAATCGGCGCCAGATACCTTAATCTAAAGCCGCCCACCAGGCCGTGCGTGCACAGGTA GTGTGCGAAACAAGCATCGTCGAAAGTGATTACAATGTTACCCCGACCAGCCCTCGGCCCATCGAGCGTGCTGTTCTTCGGGAGTCTAGCCTAATAGCCT CATTCAAGTGAGCTGGCGTGGCCACTTGAATAGGGTGGACCTGACACTATATCACAGCGTAATCCCATAGAGAAACGGCATCTAGAAGTTCAATAGTGTA AACCTGCTCGCGGCCATGAGCCTAAAATTCCGGTAGCTGTGCGGTAGTCAAGAATCTCCTGCGACCTATATCTTGTTCATGGTTCACCTCCTGGTTGGAG CGCCCACACTAAGACTGAGCTAATGTTTAGGTCTACGGTACAGTTAAGGCGTGAACTCCTGTTGAGCTACGGACCTGATGTAGGATAGTGAGTTTACTTT GACGGTGGGACCAGTTTCACGGCCGGGCGACTTACATCCTGATCGATACTAGAACTCCTTTTTTTCCTCAGTGGATATGAGTAGATTGGGAGCCACGTGG CTCGGGTTGTCCTTGCTTAAGTCATACGCCGGGGCTTTACCCAGGTCGGACCCCAGTTGTATGTCCCCGTGGTCGATGGACGGGCGGGCGGCTCTTGTGT AACTTTAGTGACCGGCATCAGAAGATCGGTTTCTTGGACACCATCCACGTGCTACTATAGATGAGCCAATCATTGATCTAGGGGGATAGGGTGGTCGCTT GGATTGCTCTAATTTTTCTAGGTCGCCCAGGTGGTGACCCACCAAGTTGAGTAGTCTACCGATGAACAGCGGTCGCCGGGTCTCATTCTTCTAGCGGCGG TTCAGGAGATACCTGGCCTCGGCATTCCGTGTCTCTACCGAATACGGCTGGTACAGTCGACATTCCTCCAACGCTCCATAATCTCCATGCGTATATTGCT AATCGCCTGCCGTGTTGAGGTTAATCCATTGCCCGCACCACCGGTGATTACGTTCCATACCACCACCAGCGCTACCCGCCACCTGCAGCCAACCTGCAAG CGTTGCATTCTACAACGTTTTTACGCGTTCCGGAGGAGACTCTCACCATTATGTTGCGACTCACTTTCTAGAAGTTCGTCGCCAAAACTACTATACGACC ACCTACAATCATCTCCCCTATGTAAACGTTGGGCAATCACTAGCCTAGTTTGTTCAACAATCACTCCAGCAGGGCTGGGCGTGTAGCCGCATACAATCAT ATCCCCGCCAAAGAAGGGTTCTTACTTACCTGCAGGACCATGTAGCTGTGCTCCACTATTTTCTCCCCAGCCTAAGACTTAAAGTAATATGGTGTGTGGT ACTATCAGGCTGAAAACGTGCGTATATTTGTTGCGGGCAAGCTCTGAGCTCTAAGGCTAGTACTCTTACTGGACACGTCTGACTAGGTAAATGACTAGGT TAGAACATTACGCAGTGCATAAGCAGACGCCTTCGAAACTTCCCCGACTTCTGCCGGCTGCGAAGACGAAATAGCATAGCTAGCTCCATAAGAAGTCGAT GTTCTGTCCCGTCCCGCTTTCTCTCCGAAGAAGGGGAGGTTTCGGTCTGTCATTGGAGTGCATGCTAGGGGGAGTTCGAGAAGGAAGCGCCGCCCCTACG CCCGGGCCGAAGGTTCTTCTTTGGTATCGAAACTTGCGTAACAGTGGCGCGATTGATGGGCTGCTACCTGCAGGTGCGTGTATTGCTGAAAGCCCTTTCG TCGGCTACTTAGGAAAACGAGTTCTTGCGTGCATTCATAGATATGATGGTTGGGTAGTATTATTGTTCCCATAGCGCTGTCACGACACGTCAAAACCTTA TCGTCGTACGTTGAAACTCCTAGCGTCGTGACCTTAATCAGGCGAACTCCTGATATACCGCTCCGGGCGACATGGGTGCATTTGGGCTTCATCTTGCCCC TTACGCATGTACCGACCCCAGAGCGGCCACATGCTGTGATTGGACCAGCACGTAAAACGACTGCTCTAACTAGGTCCACCCACCGTAGGAATAGCGCCTA AATGCACCCCCTGTATTAAATACGTCAGTACCAAACGGGTAAAAGGCCTTCGAAGGTGGCAAGAATTTGACTTGCATTGATTGTAACCCACGAGTGTAAT GAATTACGTCAACAGGGGCCGGATCCAGGCATAAAGGCCCTGTCTTCACCAATGGACCGTATTGTCCTACATCAGACGTTACTGTTTTATATGAATGTTT CGCCTTTTTATATTAAGGTCGTATTGGTTAGCACTTGGTGCAATGGTTGGCACTCAGAACCGACTAGTTCGTACCCCAAGTTCTTCAGTGATACTGTTCA CAGAGCGATCTACTACAGAGGGGCCTGAAATAGCTCGTATCCCCGAAGGCTCAGGAGGTGTGCACCAAACTCGAAGCCAGGTCTGTGGGCGATAATCCAT TATTTTTGTAAGTAGGCTGGCTTTACCCAGCGGAGGCGAATGATGTGTCCATCAGTGCTTGATACTTCCCTAAACTATGTGCGCCCTGACGCCAAACGAG CTTCTGCTTCGGTTCCTCGGACGCAGGCCGGAAGGAGAGAATGCTCTGGATATCTCTTGGGCGGTAGCAAATAAATGGATGGATTTTGGAGCGCACTTCC GATGAGGGTTTCCTCCGGTATCTCAGTGTAACCATTATGAATACCTGTGACACGTAGGCGGCTGGCATAGAGGGTGGGAATAGCTCCGATCCATGTGCCC AAGTCTTTAAGAGTACAGGTGACGTCATTACTCGCAGACGTTTTTACCTCTGGTTTTGTATTCTACATCAAAATGCGCGATATAACCGCGTAGGACAGGC AGGCTGAATCCTTCGATACACGTTTGTATCGCCGCATACCCCAACAAAATCCTTCGACCGGGGCCCCTCTATCCGGCTTGAACCTGTAGAAATTGCATTG TTTGCATGCATAACTACGAGTGATTAGTGCATTTCCAGTAAGGCGATGAACTTTCGCTAGCAGGAGCCTCGTTTATGGAGTCAGCCTATCAAACCCAGCA TGCTGGTCCTCCGAACCCGCGAATCGCTAAAGCGAACACATCTGACGGATCTCGAGGAAGACGGTGCGTTTCTAGGTCCAAATTAATACGGGCCGTGATT TGAACTTAGCAAACACCTTGAAGCTATATCTTGAAGTCTCAATTCGGTGCTGTTGGCGACGTACGCCATTTAGGTAGGGTTTTCATCCGTTGACGAGACA CGACGATAATCGTCATAGTGCGACCTAAGCCGAAGTAAGTAGTGGCCCTCCCTGCATAACAGTATTCCTATCGAAGTACCATCAAACCCAACGACAGAAT GAGCCGGAATGGACGTGATTGCTTGTGCTCAGAGAGAATCATGGCCAATGCTGCTAAAAGACTGGACTCGTCTGTTTTAATACCATACCGCTGGCCCTGA CCCCATCAGTATTCGCATCCAACTAATCAGGTATTTACATTATCACGGTAGACATGGGCCTAAGCAGCAGGTCCATCGCCTTGGCTGGGCCTTGCGAGTG AGAGCGCTTTAGATGTTATGAGGCGCTCAAATCTTAAACACATGTCTGTCGTGATGATACCAAACGTGAAACTAATCGAGAATGGACTTGTGTGGAGTAA TGAACCAATAAAACGACAAAGAACCCTGGCTTTTAGCTCTTGTCCCAGGTTACCCGGTTCGCTCCCTGGTACTCGCACTCATCAGGTAGTTCTACGCCCT CCAGCTCATTATGACAACGACTCTAAAGAACTATGCACAGGTAAGCTCAATGGGTTGGCAACGCTCAGCCTTTGGTGCAAACTTGCAACGGATCAAGCCT AAGCGGGGCTGTCTTGTCCAATGTTCGGGTCCAGTACCATGCATTCCATATTCTTTGGTTCCACCCTCTTACCTCACAACTCTCTTCCTTCCTTCGGGAG CATACCTACTTACTTGCAACTTCGATACCATCACATTTCTGCAAAGACCCTTGTACGCATATTGGGGCAAGCAGAGAGCGGGCCATCTAGGCCTTACTCA CCACTGCCACTTGCCTTACTACATTCGCATTCCGTACCCGGACGGGGACACTAATCATAAGGCAGATGGTCGATAGAAGGACCGATCTCTCTCGGCCTTC CTAGGCCTATTCTATGGTCACCGTGGAGAATAGCAGATCGAGATCGCGTGTAGGAAACTAGATAACCTGCAAACACACTTACCTTGCACAGGCCAGGCGA TCCATATGGCGGTTTAAGTGCCTAAATCCCAGCGATGCCTTCATTTGGTCATACGAAAGAATAGGTTCATACGGAATAAGCACACTGTTCGCGCACGTTG AGCTCCTGCGTCAGGAGATAGCCTTATGGCGGGCATACGAGCTGTGCTTTTCCACCCGACTCAGAATGTAGTGAGCCAAAAGTGAACACTGTTGTCAGGT TTCATTACGTGCCCTGCCAATCTGTCGGAGAGTGATGGGCCTCGGTGGCGTATATTCCATAACGCGAGTGTTGTTCCTAAGAGGGTCACGCGAGTACCTT GAGTTGTCTTCAACCCGCCCGGTTGAGAGGAAGCGGATTCAGCGGCATGCGGGCCACGTATTTCCCCCAGGCTGAGGCCCTGGCAATGGGCGGGCCCGAC GTGTACCCTGTGCTGGTTGCAGGGGTTTTCAGCGCTATCCTAATAAATGGACGCTTTATTCAGCATGGGTATCGCACTTGGTTAGTCTATCCTGGGGTAG GTGCTGTTTCAGGGGTTGTTGACTGTACTAACAGCTTAACGTACGGGGGCGTAACAGCTCTAATGGTAGACAAGCAGGTCGGCCACCTGTGTCCTAGCGC TTAACTGACGTCCTCCAGCTCCCGTTATCCACCCCACCCAGAGCACTCGATAATCAGCCTCGCCTAATTGACTCGGGTTTGACGGTAGCTAGATAAGGAG CGAGTAAGGGGACACTATTATATGAGTTTAATGGCCTTGGCCATCGTGCGCGCGGGCGACGGAGACTGTTTTATTTGAGGACTTTCTTCGGTGAATTACG TTGTCGTACAGTGACCGTACACGCATTGGATGCTAATGTCTTCCTTTCGGGACGACACCCCTGACGTACCACAGCACGAGGCGCCACATCACACGAGGAC GCCGCCGAACTAAACTTCGGGGACACGTGGTCAAACAGATTTGCGCGGGAAGGTTCCCCGCCCTAATGCCCGAGGGGTCAGAATCTCAAGTTATATCCTG ATCGGTGTCGCGCGCTTTCAGTTGTCGCGGGTCGCGTATCGCACGAGGCATTTGTTTTCGCAAGGTCGTTCGTGGGGTGACCTCAGTGAGCTGCTACCAG ATAGGGCGTACAATATACCGATCCCACTTCTAGATAGTCTCAAGCTTAATCGCAACCGGAGTCGTTTTTTTTATAGGTAAGTAAGACACCTCCTCCGGGG CAGGGAGCCTGGGGACGGCATCGGGACAGGATGAAACGGTTCGGCATTTACATTAACGCGTGGAGTGACCCATGATTTAACTCGTAGATACAAGGTACTT AGTGGTCCACTTCTATCGCCTGGCAACTAGTAGATTACTGAAACGGGAGGTATACGGTTAAATCTTAGGTAGAAGTCTGGATCCCCTTTCCCGCTCCGGG GTGCGGGGGACTCAACACAAAGGCCACCGCGAAGCCGGTTCGATACTGGCCGGAATTACTTATGTCCCACTGCCGTCTCTACTCTTAGTCCCTTTGGTAA GGAAAATTATGACCGAGTTATCCACTTTGCCCTCCTAGTTTGCGTGCTTAGCGAACTCGCGGTATGTACTTGACGATGCCGCTTTCACTCGGCGTTTCTG ATGTCCAGATGGTACAATTCGTACACGTATCACCGCCACAAGCAGCCTAGCCGATAGCGTTTGTCTCCCTCACCGATTAGAGTAAACAGAGCTGCAGCTG CGTAACAGCCTTATTTGCTGCCCGTCATTCTTCCTGTGATTTATTAACCAGGTCTAGTGGAGGCATTCGCTTTCGAAACTAATAATAGCCACCCGCCTTA TCCAGATGACTGCGATCCGCAGAGCCGCCTACTATTCCGTCCTACCCTCGTAAGGCACGGCTAGGTCAAGTCAATACATTCCCCGTACCCTTGATGTTGG CTATTGGCTTCTCTAAAACTGTGGTTGTGGCCAAAGGCGGTAGCCACAAAAATTTATCACTCATATAAAGGCGAGTTTTTAGCAAGATCCTGACGTGTCA CCCTAGGGAATCACAGTATCACTGACTAGCTGCCCCCCGTAACAGGCCAGAGTTGCATCCCTGGCCCACAGCTGCAGTTATTTCCAGCACTTAGTGATAA ATACGGTAGGGGGTTGCGCTACAGTCAGGACTCTACAGGAAGTCGGGCAGGCGTGTCACGCTGACTAGTTATGGAAAAGCACTTGGGGCGGGCAAACATC ATCTGCTGCGCGAAATACATGCGTAATCTCCCGTCGGAGTTTTCTCACTGAGCCGTTTATAAGATCTTGACTAGATCTCCTCTCACCCTATATACTGTCG AGTGTCGAGCTCGCATGACCCACCACAAGTATGTAAGGAGGAACCATTTATCGAGGATTGCCATAGATGTGATATGCTCGTTTCCTTGTGAGCACTACTT GGCAAACAGAATGTTCAAACAACTATTAGGCCTCGGATTTCGATGGCTTAGAGAGTGGTAAAAACAAAACAAAAGCAGACCACTGGCTGTAGACGCGGCG GAAACCTACCTCTGACCGGGACGCACAGTCATAGCGCCCTTTCGCAATCCATACTCGCAAGTGACGGTCGGATAAGATCATTCAATACTATCCGATTTCA AGTTAAAGTGAATTGAGTCTACGTTGCACTGATGATCCAACTTCCTACTTGAGGTAAATGACAATACGGTCTAAAGCCTTCTGATCGCCCGCTATCCTGA GGACTAATAAGAGCTATGACTTTGAGGGAGCATTAACGGACTTATAAAATTTATACACCCAATCTTCTAAGCAGTCATTGAGAATCGTCCGATATGCGGG TACACTTCGGGGACCGGTTATACCTACATCCCTCAGGACATGTTGCGACCTCTGGCACCATGCCAACAACTTACTACTCAGCCCTTACTATACGGAGCTT CAATAAACTGCCGTTTTCACGTATTACGAGGGACCTCATGAGCCATACCTACCCAATTGGATGAGCAGGACCCGTCTTCCCTGCCCGATGACTCCCACGT CACTGTTACATGGCCTTTCGATTGGTTGCTATTGAGTCCGTTTTCGCCTGTGCGAAACTGAGAGGACCTACAACACTCCTCCCAAATAGTGTCCGTGGCT GGTCGTGGCGATGACTTAGTATGCGCGGGTCTCCTTACTAGGAGCATCGCCCGCAGATTGCATTCACAGCGAATCAACTTGAATTCTCCACCCAGAGGGG CCTCAGTATGATTAGTTCTCACTCGAAGGAGGTTCTGAACAGCCCCCGGCCAGAAGCGAACAGTAGTAGTCCGGGGTCAGGAGTCGGAGTTTCCAGAGGG GTAGGTAACATTGAACGGACGCCGCATCTCCGTCTCTGAGACTATCCGTTATAGTGGTAATTAAGGACTCAGACCTAGTTCAAAAGAAGGTCATCCAGGT CCTACGTTGGTGCGCAATTCTCCGAGTACGTCGTCGCTAGGTGATACAAAGAACGGTATCTGACAGACAACTTTTACTGAACACAGCAATATGTGCCTTT CGCAGCCTAAAATGCCTAGTCTAGCGACAGGGATAGTAAACGCTATTGAATGGACTCAATACGTTCCGAACACAATCTTATCGGGGAGACTGCGATGAAG AGAAGTTAGTTTCAGAAGCGATCGAGTCGAATTGTGTTATTGGCTCTAATGCATGAGTTCATTTATAGCACGTCCCTGCTCTCTGTAATGAAGTTCTACC GCCTTAGCAACGAGCGAAGCGCGCAAGTTTTTGGCCGGCACTATCATGCTATACCGTGAAGCGCCAACTAATGCGGGGGTATTACTAACATACCTAATAA GAAGCGACATCTCTGTCATGCACCGAAAGTCTGGAGGATGGGCGTCAAGCAGTGTGAGAGGGGGAACCAGTCTAGGGTACTTTCCAAGGGATACGCCGCG GATAGATTTGCGTCCTGGATAACGTGGCCACCACAAGATTACCGTGAAGCTGTGTAAGTAGCGACGAGCGGGCTTTACAGATGCCTCGAGTTGTTTGGTG TATTCAAACTCTTATTGCTGGATAAACTGTCACTTCTTCGTAGCACTACAATTACCGACGACGATACAGGTGACATCGGGCGCTGATTAGTAATGAAATG CAGTTAAGTTTTCCCTTTGCCCTCTACGTAGTGCGAAAGCTTCGACTGGAAACATAGACGTTGTAACCCCTTCGACAGAGTCGTATACCGGAATCCAGAA ATAGACAATCGTGGGCCGGTTCGCGGGGAAAGGCAGCTCGCTATAACGATAGCTTACTATTTATAGCGTCGGTTCCGGTACGTATCCCGACCGAGGATGA TTGGCCGGGTACCGCTCAGACCAACTTCGTAGACTGCTCCATTGGCTACCTCCAGTTGTTTCGTGATGGGCACGCATTACTCAGTTGTTCCACACGGGCC AATGAGAGTAGCCCACATGGGCCTAGGCGTAGTCCCAGCTCTTTGCCGGATACTATACGCAGCGATGCGTTAACGTGGCAGCAAAAAAATCCGCAATCGT ATTCGGCCTCATAGAGAACTGTCTATGAAGGGGGGAAAATACAATACTTGGCATCTTCCCTAAGTTTGTCAAAAAATGCGTTTTACGTTGCAGCCATGGA AGTCAACGAATTAGCCTTATAGGTTTCACTCTTGCAATGTGCCTAACTATAATCTGGGGCCTGGGTCTGTGATCCGCGCACATAATGAAGGAGACAGGGC TGCACGAACGAAGTTAGTTCATGGTTATCATAGTGCAGAACCGCGAGCTCCTTGGCGGCCGACCCCTGTAGTCGACGATAGTGTGCTCTTGCCGAACCTG GAGCCCACGACGAATCGTGTCGACCCCTGGTCGGTCGCGGCTCCCTCTGTAAAGGTGAGTTGTTGACAGGTGAGGGTCGAAAGCGACCGGTATGATGTGG AACCTTCGCAACCCGTGAGGGGGTTTCACAGCTAAGTTCATGCACCAGTCTCACACGTGCAGCGCCCGCGCCCCCAAATGACGCCGTTCTGACTCCGCTC CAGCGTCCGACCAACATGCACAACTAGGCCCAAGCACCTTGAAGTTGAAGCGTGGGCGTCAATGGAAGCTCCTGACGATGCGATCCTCGTCCACGATGAG AATCCTCTGCCTTGCGGCGTGCCTGCTACTCACGATCGCCTCTGCCAGGGACGGGCAACTGGGGACTCAAAACACTTCTGATGGATAGGACGAGAACCAC CAACATACCTAGATTCATTTGTAGGGCGCAGAAGAAATTATGGGCAACAGAAGCATTCCCATCGACAGCGGCGGAACGACCTAAAGGTTGAATCGCCAGG GTTTCGTAATCAAACGGCCATACACGTTAGAGTGTGAGAGACCCGTAGTATCCTACTCACTGCGGCTTGAGTCAGACTCAAAGGCCCCGTCATCGCCACG TTTTGGGAAAGGAGGAACTCAACGCACGCCTTGCCTGAAGCATAAGTACGTATTAAGCTGGAGCAGGGATGCCTCGTTTGGGTAAATGTTTGCTTGTGCA GGATAATACGAGAAGATATTGCCTAAGTACCTAATGCCATATCGGTTATGTTTAGATGCAGGTTTGTTAACCAGATTTCCGTTGACTGTAGGTTCCCGAA CGTTTCAGTTCGTCTGGGTACTCATCAATACCCGGCTGGGCGATTCTCCAACCATCAGTCTACCCGGAACCCGTGGGTTCCGTCGTGAGTACCAATTAGG ACGACTAAGAATACGGCACCCGCCAGAGTCCCAACTGGAGAGCAACGGTCCGTGTGTCGAGGGAACATCTCCTACTTAGATTTAGAATGGAGGGGAAGCA TGGTTTCCCGGCTAACAACCATAACGAACAAGTACCAGGTTAGCTTGGCACCCGTGATTCTTCGAAACCCTACCGTCATTCCGTTTAGGCAGGTACATCT TGCTAGTCGAGTCCGGTATGAACCTATGCCTCGAGGCCCCCCTGTCGAGACGAGGTATCGACCGCTTGTGCTCGTTAAGACACCAGAAGCTTAGGACAGG TGTCCAGACACCCAACTTGACGTTCAAAAGCGGCGGCCCATCCTGACACCCCGCATCTTAGCATCCCGCGCCAGTATTCGCTATGGGTTTAGGAGTCCTT GTTGACGTCCGGAGTTTGATGTTTATGCAGGTGAAGAAGCAACTGATTCTGCAAGCTGTGACTGCACGCTGTCTCTGATGCCTGCCTACCCGGCTAACGA TGCCTTCGGGGTCGGTATTTTCAATAGCGATATCCGTTTTTTCGGTTGCACCTGACAGAATTTGGAATTCTTATGCGGGTAATAGTGTCTGTACTCCACC TTCAGGTTGTCTCAAGGCGACTGAGGGATATACCATAGCGCACTCAACCCCTAATTCGCCAGCAATCTAGTCCGATATTTGACAAGATGCATTTGGTCCG CGTGTCGTTAGCGTCGTCCTGCATCGGTACGCCAGATACGGAGTTAAACCCTTGGATCGCCAGCTTCCAAGGCGCAATGCTACTCAACTATGTGATACTG AGCGCGGTATGTCTCGATATCCACTGACCTAGACTTTGAAACTCTGTCAGTAATGCTCAAACACGTCTTCCCATCACCGGCAGCGAGTTAACCGCTTATT CCTCGATACCAGGATGAGGTGCGCTTTTAGTGACCTTAGTTGTAATCTCCATCTCATAGAGCACGTGTTATGGAATTACTGACTATCTGCTTCAAATGGT GGCCAGCCCACGTGCACGGGGTAGTGGTTCGTATTAATCCTCCCGGGTTTGTTCCTCTACGCGTCAGAAATGGAGCCGATATCCACAAATGATATCTTAC CCAGTACGGGGCTTATCTTGATCTTAGTCAGGCTAATTGGGACATTGTCAAAACAACAATCGTGCCCCATTGTTTAACCCAGAGTTCAGAACTAAGCAGC GACTATGCTGAAACCTCGGCGGAACAACCGTGCCCAAACGCTGCAGCAACGGTGTAAATTCGACGTTTGGTGTACATATTCCCAAACCCATCTAGAGCGG TGCGTTATTATGCCTCTACGAGACACGTGGGGTAGGCGAGTTTCGGCCACCAAAGCTGAGATACCCATCAAGTTGAGCAAAGAGAGACTTTGTACCCGAG AGTGTTATAAAAGGAACGCAGCGCAATACCTCTCCGGCACGGATTGAACGAACCTATCGGAGCATATGTTCGTTCACATGGGTGTTGCTGAATGACTCAA CACTTGATACGTTTATACGCGTGTTTAGACGCTTGTACGATCACCCACCAGAATCTGCTTCGATCGCCTGGGTACTTGGCATGTCCAACTCTGGCTCCCC GCGCGCATCCAAGCACACAAACATGGAGGGGTGCGTAATCCGCGCACAGTTTATGACTACCGGTCATCTCATACAGGGAGAGCGCATACTGTGTTAAGCT AGCATAGTGCTTCTCAAACAGGGTCCCCAGAATGTACTACGGTAGTTATAAGCTACAATGTTAGTGAGTACCTGTCCCGACAGAAATCCACTTACGTCTG CTACTAGAGGTTGCAAGATCTGAAGTGGATGATGTACCGCGTCAGCGGCTACTATGTGTTATTTTGATCGTGGCGGTAAGTACCGACTGGTAACCAAGAT ACACATTAAACTGACGAATGATTGACGCGTGTAAATCCCGCGAACCTCTGAAGCTTTGGTTTACGTCCACAACCAACGCTCGCCACAAGCGAATACGTGC GGCTCTTTAACATTAAGTCATGTGTTCTGGCACAGTAAGCGGGCCCGCATCGCTCACGCTGCATCACCGGTGGACTGCTGTGTCACTGAAGAACCAACAG TAACAGAAAGGACTCCAGATATTTGGCGAGATCGCCATAACAGACGCGTATGGTGAAAGGTTCCTTCTTACCCTACATTCGTCAGTCCGCATGTAATGTA AAAAATGAGAGTTTCCGACGTCGTAGAGACGCATCGTCAATCCCGTTTGGAAGACTCTGAAAAATTAGGCGCAAAGTTGATTGTACGCTTACTTTGTCGG TGGTGCTGTCATAAATGAGCACTTACGGGTGTATGTCCTTTTTGAGGTGCAAGCCTAAACCCACTAGAATGACAGGCCGAGTGTGTCGGACGGTGCCGCT TCAATACCGGGTTCCAGGAATGCCGAAGCGTCCTCTCTGCGATATGTAGCTACGCGGTCGGGGGCAGATATTCACTAGGTTGTCGTGGGCCAAGGTTCTA AATACCTAACCGTTCAGGAGCGACAACGCCGAATAGACATTTATATATTATTTAGATTGCCCTGTTATCACCTGTCAAGGCTGACTCCTTGCCATAAGAC GACAAAATACATTACGCATATACTACTCAGGGTGCGACCAGGCTTAAACCGAGGCAGAACCATCCATTGCTTAGGCGATCACTAAGACACCGTCGCAGTA TAAGTCTAGCCGGTCGCGCGGCAGCCTACTTGTTCAAAGCATTTAAGTGCTGGTGATATCTAGAAGGCTCACGCATTGAAATACGGCTCTCCCTCAAGCC AGGTGACTCCTCTTTTTTACTAGCACCTTAGTTGGACAGTAGAAGCTCTTGAGTTTGGTGTGCGCCGGAGTTACAGGGGTAACACCATGTGGGGATTTCG CGCGACATTAGCAAGCCGGCTCCCCATCTAACAGCACACTTGCGACTGTCGTGAAACAGAGTCGGCTAACATACTTCTGGAAAGTTCTCGTCTCCACACT GAGACTGTCCGAGGTAAAGTATATTTAGGACCATGTTAACCCCTCGGGCCCCGAACATGCTTTAACGAGATGCTGACCCCAGAGCTCTGCGAACAGTTTC CTCTAGGCATCCCTCTAATGGGCTAAGCTCCAAAATGTAAGGGACACTAACTTTCCCCCGGCTGAAACAAGATTTCCTCGGTGCACTCTTAGCTAAGGGT TCTTTAATCCTCCTCACCGAGAGTACATATCGCTAGCTTAGATGTCCAACTGCGTAGACATACACTGAGTGGGTTTTAATCTCTTGTTGCTCTTTGAGTC TCAGCTCGTTTTTAACTAAGCAGCTGAAAACGAATACTTGCTCACAAAGTAAAGACTCTACCGCTAGGTCCTGTATAGTCGAAGTTAATCCCGTCCTACC CACCCGCAAGTCGGGATAATGTTCCGCCTTTTTCTTACACAGCTTGTCAACCGCATATTTCGCGAGGCCGTGGGTGGGCGGTTGATGCCTGTTGGTGGAG TAGCCTATCAGACGGTTAAGTTTCAGTGTCCCGTTGGTTACCAGTTTTGAGTAATAATGGTCTATTAAATTGCCAGGGAAAAAGGCGCGATCGAGCTCTC AATATGGTCAGCCCTTTGTGAATTCAGGAGTATCTAGATTTTATTGGGGGCGTTAGATCGCTGGCAAATTATTTTCCAAAGCGATCTACTTTCCATGCTC GGTAACACGCCGCCGCAGAACGATATTCGTGGACGGATGTAAGAGGGTTCCCGATCGTTTATGTGCGGACATTATGGTTCCTCTTGAATGGGTGAGCTCT CGTTTCATACTAACTTACTCCTTCAAAGGGACAACAAGTCTTCAGGGTCGCGCCACTTGTTTAGTATCTCCAATCATGGGATGAATCGGGACGACACATG GTGGAAACAGACGTTTTGGTGTTGTTCGAATGGAATCCCGCTCTGGGACACGTCCCAATAATTGCAAGTAGAATTCGTGCTACCGCCTCACTGCTGATCG TTCCTGTTGCGGCGTTCGACTTAACTTATACGTTCTTGAGCCCGTTTATTCCCGATTATTGTAGACTACGGAATATTGTAATCGTTAACTGGGCCGATGA ATCTGGCCTGTGTAACTCTGTGGTCAGAAGTCGTTTATGAATACGAACTCCACGCCTATTATATTACCTTTCTACAGTTAGGGCCTCGTGTACGAAAGGG TGCAGCATCCCAATAAGCGGCCCGGCGCTGTCGCGGGTTCCGACCGATTCAAATGAACACTTTCGATACGGGCCCGCAATGATAGCTAGAGAACCTGAGG GACCTAGCCCACCGGGCCCTCCTCACGCTGAATGAGTTACTCGCGGCGTCCTCCTTTATTGCATAGTTGTAATTATCTTCGCTGTATCGTGCCTTGTAGT ACAGCTCGGATGCCGTCTATTATGGCTAGTATCCGCACCCAGATAAAGTAAGCCAGGTAGGGACTTCAATAGAATGGTCAGTCCGAAAGACCCTCTAGTG TCATGTCATTCCTTGTCTCACACCGGTTATATCACAGACATTTTACAAATTCCGCGTAGAAAGCAGTTCACCTTAGGGAGGCTTGGGATATCGGATAGCT GGGCCATGACCTGTAATCCGTTGCAAACTAGTCACGGACTGTATTCCTGGGAATCGCCAAGTTTAGCCCACCGGTCGGGAAACACTATACCAGAGGCCGT AACTCCGAAGGCTCCGGGGCACTAGGAATCGTCGGGCATGCTGCACTATTGTAACCTCATCATTCAATAGGTAAGAGTGATCGTCCCATACTCCAAACTA GCTCGCTTATGGTGAATTGGGTTCGTTTGTCTATCTCAGTCAGATGTCAGATGTCGCAAACTTATATGACATCTCGGTAATTCGGGCCCGACTGTAGCCT TCTGGACTCAGAGTCATTAAGCTGAACCCAGAAACAGCATAACCGCTGCTAAAGAGAAGATTTCCGCTAACGCGTCCCTTTTGGAAACTGAGTTCGGGGC CGTCTTACTATTGTGTTTATAAAGCTACGATTGGTTACACAATTGATTGTCGACTCGAGTGGCAGATGAGTTATACAAGACTGCCCAGAGGCCCGATTTC CTTCATAGTTTCCTGTACATAGCATCTCATCCAAGATGAACGTCACTGAGAAGTTCGTTCCACATTCGGCAAACGGGTTCCTCGGAAGGCGGCGTCAAGA ATAGTCCAGTCACCGGGATCCTGCGAAGGTTGACAGATGCTCGACGCCATAAGACAGACAATCGCGATACTATAAATCATTCACTATAAGAAAAAAGCCT CCCCTGTCCAAGTGCCCAATGTATGTCTTGCGCCAACTAGCACATGTAAATCTCGCCTGTGTTGTCACTTCGATGGCTGAGCCACGTGGGCGGGAAGCCA ATCAGGCAAGCAACGTGTATAGGCAAATATGTCTGGAGTGCGTGTGCTGACCCATCGGCGCGACTACAAAAGCGTCTTACGAGATTAGGTGCTACGGAGG GGATGTATATTGTATAATGAACCGGACTTAGGAAATTGTCTGGGGAATGAATGAGAGTTTCTATCAGGGACCGCAAAGTCCGGCACTGAAGTAGCACAGT CAGAGGGGCCGGACGTGTCAATACTGTTTAAAGTCTTCTTGAATAGCTACGCTGGCAATAGGCGGCGCGGCGAGAGCCCCATGTTTATTGGACCGGCGGC ATGCAAGCCTGAGAGTGAAACGTGGGGCCGGGTCTCTAGCCTAGAGTTACAGAGAAGTATGAGTAAGCAAGGGTCGGAACGTTGTTGTATTCAAGGGAGC GTGTATCGGAGACACCTAGGACCGCGAAGTTTATTTGTGCGGTACTGCTGACTCAAAATGTAAGATTAGACATGAGTCGATGATCTGCATACCGGCCGCC TAAAATATGTTGCGAATACGGCGTGCACTTCCACCAGACTCATCGGGGCCAAAACTAGTGTCATTGATAATATCGACGCCTGTCACGTCTTGTTAGCTCC TTGGACGGTGGTTCTATTTCTTGTGCAACATACCGACTAGCACCACTACCCGAACCACGGAGATATGTATAAATCGCCAGGCGAGAGAGGAGAACATAGC ACGGGCAGTGGTCGTGAGACAGATAGATTGGAGAATTTGTGAAATAGACACGCTTTATAGAAACACGTTTCGTAGACGATAGTGGGATAGCCACGTATCA ACGAAGCTTGGGATCCCAGGGTGCGTAACGGGTCTAGGGCTATTTATATGGACGAGTTCGGCTACCGTAGAACCGTCAGTCATCATCGGATAATCGGTTG GGGGGAGACAAACTACAAATCCTTCGAGGCTCATGACAAAACAAAATACGGGTACATATTAAGTCTGATTAGGTTAGGTTTTAGTTCCGCAGGGGTCACC CGAGCCTGATGAAACACCTGGGGCCTTTACGATATGCAATGAAAACTTGTACGATCATCATGCGTATATAGTAGGGCGAGCTAGGAACTGCAACTGAGAG CAATAAGGACAGGGAATACGTTGTGATCATCCATGGATACAGTGGCTTGACCACGACCTCGTACACGGGAGCCTACGACGGAGGGCGTCGCCGGACGTAA TACCCGGCGGAACCCAAAGACTCCTGGCATATGGTCTCCCAAAAATGGCGCTGATCTTGACGTCGGTTTAAGAGTATTCCTCCAGCCATCATTCCTCGTA TTTTGGGTGGTGACTACCCCTAGGAGCCCGATAAGCATGTGGCGTTGCTTAACAAGTTTTTGAACTTTATTATCTGCGCTCACTTCCGACGTCGCGGACG TAAACTGTTAACCCCGTGAGGGTGTTCTCGTTGAATTATCCGGTCGCAGTGCAGTATAAACCAGGAGGTCGAATCCACTTTCCGCCTATCCCGTACATCT TCGTGGAGCGCACGGTTGTTGTACCGAGTTCTGCGAGAAAGATCCGCCGTAAGAATAATTTGTTCTTGGTTGGCACGAGACCTGCACACCGCCAATCCCT AAGGAGCGTCACTTTATTTTTAGATACTTAGAGACGAGTGTCTTACGAGATATGAATCATGATGAGACTCCCCCGATGGAGTGCGTGCAATGACACCAAG ATCCAGGGCGTTCTGAACCTAGTAATAAACAGACAGGTAAGAACCTCAGAGCTTAGCACTCAGAACTCCGAGAGCGACCCAGCTATAGCACTTCTGGGCC GGATGCCGTAGGAGGTCATCTGTTATAAGGCTACCCGTAACTTACACTAGTCATTACGTTTGTATTTTTCCGGGTTGACAAGAACGCCCATCTCACCCCG GCTTGTATTGCGCCGTCAGGAAGGTTGCGAAAACCTCAATGCAGGCCTCAGCGCGGCGAAAGGCGCGACCGTGTTCACAATCACATAGCTATATGCGCTA ATAAGATTGGGGCGGCTCTCATTTCGAGAGCCGGATCTTTTGTTTGACAGACGTTGTAGTTGGTAGTAGCTACGATGTAGGCAACCACAGGGGCTCCGAA GTGAGTAGGGACTACTTAAAACATTTCCGGGGAGTAGAAGTAACGATTTGGAATACCGGGGAGGGTGGGCATCCGTCCCTTGGTGAAAATCTCGCAAGGG TTACACGACATTGAAATTCACCAAAAAATTCGCGGCGTATAGTAATCTTGCCCTACTTTGCTATGCCCTACTAGTGGGGGACTCAAATCGACTGGGTGCA GTCGGGAATAAGTTCAGCCCCGAGCCCGGGCCCACTCGTAGAGGCGCGCTCCCTTGGGTCTAGAGACATGAAGCGCACTCACGGTCCACAGTTTTGGTGG AAACGCTGTCAATACTACGTTCACTTGCCCATACGGTTATATAGGCGTTCGGAGCGCGATTGCAGGTGGGGATATAACCACAACGCGGGGGTGAGGACTG AACATCCATACCGGCTTGTACCGCGTGGGATCAAGACACTTGCTATCTGATCTAGCTTTTCGAAGTATTGCTCGGTGAAAGTAAATTGTGCCAGGTAGAC TTATCACGTCCTGATACGGGAACATGGACTTGTTACTGCGGCGTTGAAATCAATCCTTGCTCTTCTGCCATGTGTATTGTGTTTCCACTCTGTATTTTGC CAGCGTCGTACATGGCACAGCTTTGACCCGTTGCAATGAGTAGGATATACAGTGTACTCGCGATTAAATGGTGTCACATGCCTTGGCGCCATGCGGTATA GTTCAGGCGCTCGTATTCCGGACCCGCTTCAAAATGGCGGCAATTGGTTGTCGCTCCCCGCCCCAATGGTATTGTGCCTTGTCACGATTCACGTTCCGTC TGATGAATGAATACTTCCACGTAGCACGCCTGCATTGCAGCTCTTTCCGCGAAACGTGACTATCGCGGGCCGGGTATCCTATTAAAGGAAGCACCACCTT TAGCCTACGCTATAGCCAGATCAGCCACAAGACGTTTGACAAAGCGAATACCATGACATAAAGTCCGACAGGGCGGCAATGAGCTCAATATATTATTATT ATGGACAGATTTTTAAGGTACGGCTTCAAGTCGATCGCACAACAGTGCTGCTTCGGTTCCCTGGAGCAGGCGTACAAGTACAAGCCTTTTAAGGAGACCA AGTTTATGAATATCTTGAGGCCCCCGCTCAGCAGTACCATTTATACTAAACGGAAGTCGCCTGGACAGTTAATTCGTACCTATATAGCCTGCTGGTAAGC GGCAAACCGCTGTGTCTGTACCATATTCAGGGAGTTCACGTGTCGATACCTCGAGTGCGGGAGCGCACCCGGGCGCCCTAATCGTGGTCGCCCTAGGTCT CCTCTACTATTGGGAAGCCATAACGCATCCTCCAGGGTTTGCCGGTAGCTTCCTTTGGTCGGTTAGCACTTCACTAATTTCGCGGTACTATTCAGTTGAC GTCTGGCTCTCAAACGTTCTGGACTGTTACTCTTGCGGAGTTTATGCCCCCGATCTTAATTCGAAAATAGGCTTCAAAACGCTCCTTGTCAGGCCAGATA TTCGTGCTGTCGGTGGCGCTGCAAGCGCAATACTGAACGCTCGCAGGTCCGATGGGCCCCCTGGTCATCTGTTCATCTTAGCAATAGAGGTCTTAATAGC AAGGACGTTACTATGTATCCTGCCCAGCTGAAGAAGTTCATCTCTGCTCACGTGTGACGCTAACTCGATCCATGTTAGCTCACTAGCGTTAGGCTGGGGG TCTCTGACCACCATGACTTCTCATGCGGGAGGGGCCGGCCCGATATTCATTGGGTCGTCGAAGCCCCTGCTTCTGTGACGACCTCGGTGGCCCAAAACGC ACTGGGATGCAATCGGCTTTCCGTGGTCTCCCAGTAAAAACCGCCAGTTATTCTAGTTGCTGCTCGTTCCCTAGGTGGTTAAAGGTCCCCAACAACGAAA TGGGTGAGTCTTACATTACGGACCTGGAAATCTACAAAAGCCTACGTACAAGTACGCACTTGGTCGTCAATGCCCTAGGCGGATGGGGACCCCCCGCCCT AGGTCCCAGATCCAGCTTCTTTGACGTCACATGTGCCATTATGGACTCTGTTCCGATAGGTCAATAGGAAGAATCACTCACTCTGATAAAAATTGACACC GCTGGGTCCTAGCTCGGCAAATGCAATCCCCCGGATAAGTCAACTCACGGGGCTTGCAACATCGCTTTGGAGAACGACTGATAGGCAACCGATCTGACGG ACACGGCGCTGGAAAACTCAGGCATTACTCACCGTGCGAAATTCCTTTCCTAATTTCTTCTAACGGGACCTCCACGATTGCTGTTTTTGTATATGTGTTG CGAATGTTAAAACAGACAGGGATCAGACCTCTAGAATAATGGAACATTTAGGCCAAAGATCGCAGCTGCATTTTTTCCTCTTAGCTCTCGCACCTCGTTT AAGCATCGCGGAGCCTGAACGTATTCACTTGTATCCTCACCATTCTGCGTCATTATCGAGTGACCCCTGTCGAGCCAACGCGAGCTCTCAGTCTCGCGAA TTCCGACGTTAACCAATAGTCTTAAGATTTTAATATATTCCGGAAGGTGTGTTACCCATTGATCACCAACGGTATGGGTGAAAGCTGTTGTAAAAGTCAG ACTAGTTTAGAGCGAACCACATGGGGTCACATCTCATTAGAGAAAAGGACACGACGGCGCCGCTTGAGGCAAGTCTTGTGGGCTGTATGGACCGAGACCG ACAACCACTTTCTAGTCATGGTTCTGAAGTTTGCATAGGTAAGGCATGCATAATTTAATCAGGTCTACTCTGCCGTTCGGAAAGGGTTGGGGGACGCATG TACCACAAAGGCCGGACAACCCAATCATACGCTGCAGCGTACTTCTCCGTTCATTTTGCGACTATTAGAGACCCACGACCTGAACAACTCCGATTAAGAG ACTGCGGGCCATATCCTGCATTGTCCTGCGCGTTACACATTTGTCTCTGGCCTCATCCACAAAAGATCTCGCCGTAAGAGCCGTGCAATTACAATGACGT TAGCACTCCGGCTCTTACTTCGCCTCCGGGTTAACTGTGCAGGGGAATCCTGGAGCATAGCCTGGCGAAAGCTGTCCAATTACGCCCCTTATGCGTTTCA ATCATGCTAAGGTAAGGTACTTATTCTTGTTAACACCTCCTCCCGGGCCGCTAACTGGATTAAAGGCGCGAGTGCGGTCGAGTTGCGTATGCGAAGAATT TCTATTGTACTTTGTGAGACCCGATCCTAAGGGGCCGTCAAACTCCGTCGAGCGAATGAGCATGCAGACACTCCCATTCAACGCCGGGCCAATCAGGCCA TACGCTCATCTAGCAAGAGGTATACATCAGCACGCTTGGAAGCCTTTCCGATAGGATGCGCATTAATGCTTGTAGTGGAACCCTAGGCTAGTCCAGTCGT GTGACCGCATCTCCTCGTGAGTGGACCGCGATAAGGAAACGTGCAGGCGTCGCAAACGATACCGAGCATCAATGGTCTGAAGGGTAGCAAAAGGTCAATA GATGCGCTCTGCTGTGTAACAGGAAGCTCGACCATGCCGTCAAAATATGTTACACCCGAATGTATAATCACTCTTTTCAGATTTTCTTACATAAACACGA AGCGACTGCTTCGGATGCAGGCGTGATGTAGCAAGCCTTTTACACGCGCAATTAGAGCTTGCTGTAGTTGTGGATCCACACCGTTTGAGTGCTTTTGCAC ATGTCAACGCCAAGGGAATCGTCGCCTGGTAAGGCTCCTCATTCGGTGCATTTCTACACAAAAGATCTTAAGCTTAGAACAAATCAAAGGAGTTCATCGG ATAGTACGAGAGCACAAGGGTACTAATCCGCTCCACGGCCATGTGTGCTAAAATCGACCAGTGCCAACAAATACCGGGAGGGACGTCGCATACTTTGATG GGAGGGAAAAAGGGTTTCTCAATAGATGTGAGTTCGCTCATGAAGCGTGGAAACCTCCAATTATATGTATTCCAGTACCCTCCCGACTGGGCCAGCGAGC GGGTTATTAGAGCGAACCTAGACAGATCTCCCTATGTACCCAATACGCCATCTGCTGAGATTCCGGTTTGTGTGACACGGCGAGCCTAAGTGCTCGTGCA GGGCCCCCTATACTTCGAGTTAGCTCACATGAACGGATGACCGATGCAGTTTCGCATACTAGGTGAGGTGCCTACGCAACTAGAAGGGATACGCTTATAG CTTCCCTGGACTTCACTAATCCGCGCAGTATAGGGTGTTATGAGAAGGCATGACTCTGGCATCCATGGATGGTGGAGGGAATTCACCAATGTTTAGTGTG ATCACGACTTCTCCCGCAAGTCCTGTTGCCCCTACTGCTCTTCTTAGGCGGCAGACGTTCCTGCACCGAGAGATTGCTGAGGATTAGCCTCGACTCTGCG GGTTGAGGAGATTCGGCTTATACCTCGCGCTATGATAGGGTAATGTTTACCCCGCATTAGATCACTCGCCAGTCGCATGCTATGCCTCGAGCGTGGGAAC TGGATGGGATCACCTGTCCCGGCAGGCCGAATTAGTTATGCCCGTTGAACGACGTAAACTGCCGTACAGCTCATGATCTAATATGTCCACCCGAGCCCGC TCTGGGTGATGAACGGTCAAGGAGGCAAAATAACTTACGAGACCAGAAGCCCGATTACGTGGTCTCTTAAGCACTCGCACGAACCACCGCCGCCGCGGTG GTACCCGATCCCGAGCCCCCTACCTCGGCGGCACGAAACACGCCGTAGCTGTATATAAGTGCCTTGACCTCCTCAACGGGGTCTGGTCCTGGGCCTCGGT CTGAAATTGCCAGTGTTCAGGGCGAGTGCCATGTATGTAGGGCCACTCCAGTGCACATTGGAGAAAAGCAACTCATATTGCCATCGGGTCCGATGTTCCC ACGAATAGAACAGCCGGAGACGCAACATCCCACTATAAACGGTCACCTGGGCCGGTCTTGCCTACAACCAGCGGCCGTTCTACGCGGATAACTATGTGAC ACGCATGTTTGCCACGGACCTGAGCTTAAACCTTTCTCGAAGCATACCCTGTGCCTATTTAATCTCCTTTAGGCTATCCATTTGTTATGACCCCAAAGCC CACCAGTTATTCTCTTCTCGAACGTAACGCATCAATGCGACCCCCTCTGATGCCTTTTTCGATTTTCCACCTCTAGGTCTGGTGTGGATAACAATTAAGC TAGACGCTACCTGGGTTAGCCAGTACAATCACTTCATCCCCACTTTCTTGTCACAGTAATGAACCATTTGAGGGGTTTTCTCGTTTGGTCGGTAAAATTC GTGGAACATTCTCCCGATCCAGCAATGGTATGAAGGCGCCGAGTCGCACATGCCGCTCAGATCAGCTATGTCCAAAAGGATCGTTCGTTTATGCGCGGCC TACTCCTCTTGGAATAACACTTTTGGTCCGAGAACGGCAACGAATTAAAACCCCGTATGCTCATTTCTTCGATAAATCCAAGTTTACATTCAGTGTCATG TCCAAGCCACAGCTGCCATGTATGCCAGTCTCTGAGCCTATCCGTCGATTCGACAGGTGCGCCCAAATTCCGTTTAAAGATCGCGGACCAGCGGTGTGCG ATTAACCACTATTCAAAGACATCATGGCAAAAACCCTTCCGACCCATCCCCGACCCGGTTCAGCACGGGGCGCGGCGCGCGGGAATCTCTTAATGGCCAT TTTACGGACAGGTAGCTATGCAAGGTGCCGAGGCTGAGGATTGTGGTTTCTCCTATTTGGGGACTGGTTCCTATAATGAGCGACTTTGGTTTCTGCTTCA AAGCTATAATGGTCGGCCTGACACTAACGGTCCACAACTCGTATAAAAATTTTGTTCGGCCCCTGGAAGGAATGAGTAATCATACGCTTGGTACTGCGCC AACCAGCTTCGGTACGAAGGAAGGAGAAAATCGCGTTCCACGTTCTCAATCCGACAGCTGACGGCTGCGAGGTGTTGCCGAACCTGGCTTATGCGAACAC CTAATAGAGCTCGTAAGAGGACGGCACCAGGGGTAGGGTTGTCATTAATAGCTCCTATAGACACCACGTCAGCTAACAGGGGTACGGAATGGAGGAGCTG GCTCCAAGTCCGGTGACCGACTATTGCACGCTCCATGCTCCCTCCGGCCATCCTCCTACGCGACAGGCGACGCAGAGTAAGGTAGTATCATATATAATCT GGACGTAGTTTGGATAAGTGGATATCGCAACACCTGATCGACGAATCACGACTATCAATGGCGTGTCCCATGTCCACCTGGCCTCCCAGAAAGTGACGGA CGCATAGCCAAGATCGTACTAACGAGATGCAATTCCTCATGATGACCTACCTAAAGCCATTGGGGACCTATATGTCCGGTTTCTTTAGGAACCTTTGCCC ATCATTACGGCTCGCTTAGACGTCGCCTTAATGAACCCAAAATACGAGGCAATTGCTCAAACCTACATACGGGTTCATTAATGTGGGGGCTCGTTAATCG CAAACGTAAGAAGAATTCGTCAGCACGATGCAGTCCTGACGGGGGCTATCTACGTGGATCAACTGTTCAGATCCTGTATACAGAGAGTCACTATGGCGGG CCGGTCTCATGAGAGCATCCTGTTAAAAGTTTTTATGCCCAGTCAAATTAAGGGCGCGGCTAATAGCATGGTATTCCACCACATTGACGAGACGAACAGA CAGGTGCAGGCCCCATCCGACGACTGTCCGGAGACGGAAGCCTTCCCTTACCCGCACATACCAAATCTCTCGCGATTGCACCAGGTTAATTACCCTGAAG ATCAGGCCGACATTGTAACTCCCTTTTATTTCGTGGTCGAGTCCGCTGCATATACACCGCGGGCAACAGCTGCTCGGAGTGATCAGCTGGTTTATTGCAA CACCGTACCTGTCGTTATCTGAAAGCAGACTGCTACGGAGAGAATCAAGACAAACACGCACCGCGCACACTCCATGGTTTTTAACAGTCTGAAGCCTCTG GGAGCGGTCAACTGATAGAAACGTAGCACTAGGCAACCTTGCTACGCGACATGCGTCCAGGCACGCATAATGTAGAGCGAAGTTTGTTTTCAACTAGCGC TGCAGCCCTCGCGGCTACCTTTACGGTTCAGGCATTCTCCCTCGGTCGCCAATAGAAATCGGGCAACTCGCGTAAGCGCCTATCACCGGAGTGAAGAATA TCTTTTACGCCATCCGTTCCCGCGGCTCAGAATGTAATGTCACATACAGACTTCGGCTGTAGGAAGGCGCCCTTGCACCAATCCACATGAAATAGGGATC TGTTGTCAAGCGTATTAGAAATGGCTGTGGAGGTTACGCCCTATAATAGTGGTGGGGCACTCATCAAATATTTGCCGCGCAAAGAGTACACGGTAACCTC CGTACCTTTGTACTCAAGATCACCTTGTGGAGTTGCTCGCCTCAGTCTTGCGCAGCGACGAGTACGACTGAAGGTTCCTAAGCTCTGGAAGAGATACCCC GCTCGACCCGTTAAATAGAGTGTTACATGCGCAGCAAGATGGAAGTCCTTAGATTGTCCACCAGGAAGACGGCTTCGATGGGTAAACGTGAGGATTTGAA CAATCCGCCATGCATCCGCGGTCTAATCTCTTCGGAAACTATTATCTGTGTACTTCAGTGACGCTTCATACCCGCTGCCTAGGTAAAGACTCACACTGCT ATGGGCAGCGGCGGCGGCCCTCAAATAAGAGTCTGTGCCCTGGCTAGCACTGCACTTCGCACGCAAGTTTCATCCAGTAACGGCGCCGATAGTCCTATTT CGCAGCGCTCTCTATTGTCCGGCGTCCGGGGTAAGGATTTGCAGACATCCCGCCTACTATGATACTATGACTCCTTGTATGTCCTGTCTCAGTTAATGGT TAGACATGTTGAGATGACTGGTTGAACTCAGTAGGTTATGCTAAGACTCCATCAGTTTATTCAACAATTAGAATTGGATCGAAGGGGGATACCCGGCAAT GAAGAAGAGCCCGTTAGCCAAAAATAAGGTAGCAACTACAAAGTAGACGCGCAGAGCTAAATAGGTCGTACTTGATATTGCAACATGACCAACCGATGCA AAGTCCGCTCCTGCATATTCGTATAGGATGGGGGCCTCGGGGCCACGAAATGGCTCCATGCTTCGCAATCACCGCACCTTTAAGTAATCGCCGCGTATTT CCAAAAAGCCGGCATGGGTGCAGATTAATTGGGACTTTGTCGAAGACGTTACACAATCCCGCCAACTCGAATGGTTAATGTTCAGCGGATACACGCTCGC TGGCGTCCACGGATCTATCGCTTTGCTCCCGCTCGGCTCCCGAACGACGGGACTCGAGTAACCGATCACTCCGCTTTTCTCAACCTCAGCTTTTGGTGCC ACAGCAGAGTGACACTCTCATGTTGGAGTATGGGGGTCCCTGCTAATGACCGCGGACGACTAGCAGTTATTTTCTGCGCGTCACTCAGAATAGCGATTGT ATAAGCAAGGCAACCGGCGAGGTCGATAGCGTGTAGCTGCTCGGACGAATCTATATAGGTAGGGGCCTGCCTGTTCTTTCCCCACCCTGTATAGCAACAG AATAGGCTGTCCTAATTTTGGGCCCCACACTTGGAATACCTGTAAAATCTTTAGCTTGGCTATCGATATATCATGTAACGAGCTGTGAAGTACTTTATTC AAGAGAGCACCGTGGGACCGAGATAAGATCGTCAAGGACGTTTACCAATTGAGCGTTGGTCTTATCACTCCGATCGCAGAACTATATCTGGCGATAGTGT ATCCGGTAGAGTGCTCCCATTCTAGTCTGTAAGAAATTGGAGCACGACTTTTCCAGTCATTTATCGAAACCGGCCGGTGGAGTAGTAGTAATTACTTTAC TCAATACGCTTTGACCTGATTCATGCCCCCAAAAGTGGGGTACGTGAGTCTAAGTACCTGTGGAGTGCTTTTCCGCCGTACATAGTTAAGTATAGACTTA TTCTACAGCTACCCGCGCGGGGGAGAAAGGGTCCAGAGTTAATTGTCTTTATAAACATCATGCTGACTGTCACCGCCGTAACGGCTTTGCCTGCTGCGTG CAGCCGGAACTAACCCGGACTTTGCTGCTGACTCTATACAACCTAATTTAATGGGGATCCATGGCATACATGTGTACTAGCAAAATATCCCACGTCGGCG CCCTTGCCTTGAGCCCAAGGCCCCAAAGGCACCTGCCGACGTTTCGCGCTGAGGAAACCGATTACAATTTCGCATTTCTCAGCCCCGCACGGCGGGATTG TCCATCAGCTGCCAAAGGTTGCTTAGCTTGCCATGAGCGACAGAGAGCCTCTACTCCGGGATTAGGGGCCAGACTGGCTACACTTGCACACAACACGATC ACGTCTCCTTAGTGCGCTTACCTTTCGGTTGCACACTAAGCTTTGCACTCTAATGTCAGCTCGTCTTCGGTCGTGGCCCCAGTTAAAATGAAGACCCGAC CATCGTAAGGCGGAATCTAACACGCCTAGTATCCGTTACACATCGAAAAGCCTAGATTGAGAGTAATTGCAGAATGGCCCGTCCGATCGACCCCTGAAGA GTTACCCTCGAGACGGCTTCCAGATGAGGGCCCATTCACCTGCTCTCCATGTATAGCCGAGTGCCGTTGCTATCCTGTTCTGAGCAGTCAACGTAGCCCG CGTAGAACCGACCTTTCACATCGTTTTCTACCGTCACTGGAGCAGCCTATGCGTGTCTCGCTACTTGTCGCTCCCGGCGAGGATCCAGCATGATCACAAT TTCGTAGATCAACCCGGTTGGTGTGCGATGTTTTCAGCTAACTTCATCCTAGTTCAGGGTGTTTTGGTTCATGGTCGGATCCTGTCTTAGAATAGTGTTC TGACGTTACTTCTTCGCGTAACCTGCCTTATCGGTCTTCAGCGGTGAAGACCATCCAGACGCCCCGTACGAGGGAGTCAAACCAATAGTCCTACTTCAAA ATGATGTGTCCTAGGGTTTCATGAAGACAATTTCGCTAGCCCCTGGCATATTTTCGGTCCAGCGCGATAGCAAGGAGGGACGGCTGTCGGCGTCCAAAAC GAGCGAGCTGCTAGTGGGAACCTATATAGACGTGCCATAACTGTTTATTGGTCACAGTGGGTTTGTTCGACCCATGACATGGATGGCCACGCCTCAACAT GCCTGCTAGGAAGCTGTGGGTACAAGTTACCTGTCTCGCGGTTAATAAAGCCGTGTCACCGATTATAAATGCCATCTGAACCTCGTGATTATTGCGTCCG AATACATTTCAATGTGTTACCGTGCCGCCCCATTACTGAATACCAAAAGTGCCCGTGACAGCAGCTAAGTACAAATCAGATAAGGCAATCCGAACTCTAC CCAACCCTCAATTCTTTCAAAGGAGAACTTGGTAATGGATCCCATTTCGAGCGAACTCAGTTGTGGCAAATAGTTACTGCATCGCCTTACGCTGAACGTC TACCCACTGTCGTTACACTGACATTCGTCATGAGCGCCGTTCCAAAGCAATGTGCAGTTCACCTGCATACAGCTCCCCTAATCCTGCTGATTCGCCAGTG TATTAGTAAATGATAAGGCTAGATCGACTTGACGTGTAGCATTGAAATTTCTGTTTCAATGGGCCACTTAGAATTAAGAACCGGAGATTGCTAAAAAGAC AAGACGGAGTGAGCTCTCAGCCCGTCTCGAAGTTTCTGTATCAATTCCGATGACAATTGAAGTTAGAGCCAGTCAAGACGTCCTTACATGATCAGTATAC TGGCTATATCCGTAGGATCTAATTCAGTCAGTAATTTATGCTGATAACCTCCGGCAAAGTATACCTGAGCAACAATTTAACGCTGAGGGGGTAGAGGAGT TAGTGGATTTCCCGTAGTCTAATTAGTCTCGTAAGGCTCAATTGTGCGGTATCGCTTACTGTTTGCTCACGAAGGGCCTCCCAAATTCAAGGCAGAATAT ACTCGGACGCGAGAGCCCATGTCCTCTCCAGCCATTGGACCAATTTCAGATTAATTAAGCAAACCGACGAGAGGGGCGTATGGCTCAGGAGAAACTCACT AATGCCCGAAAAGGTTGATTACATACTGAAGTCACTAAAATTTAGCCAATAGTGCGACAACTAGCGGTCTAAAATGCCTCATTAGCGAGTACTTACTCTG GTTTTGGTGCCATCTATAGTCTGCTGGCTCTCGGAGACTAGCACAGGGGTAGCTGCGTGGGTCTATGCACTGTTGAATTTCATCAGTTCCGTCATGGGTC GATGGGAGTGATGAATCTTTCTACTGGCTATGAATCCACATCGAGTTTAAACAGTACACGATCTAGTCGATATAACAAAAGACATTAAATTAAACATTAA GACCCTGACTCATATCAGATTCTGCGGCAACGGGAATCGGGTTTACCAAAAAGCGCATTGGGGCAGTGCGTGGTCAAACACCCATTTTCGGCGCGGTACT GAAAATTCTTGACCAGACATAGACAATTGACGAGACGTATAGGATGCCGAAAGACATCGGTCCACGATAGCGATAATGTAGCTCCCGGATGCGGGCTTTC GACAACAGGTCCCTGCACGGCTACGGTCTAAGGCCAATCGTTAAGTTCGCCACGTGGACGTCTCCCTGGGAGTGCCGTTGGCATCATGTCCATTCTCCGG AGTCCACGGATAGATTTATTTTGGCTTTATGGCAGTCAAGGTTCACCTACGCATAAGTTGTCGTTGCCATCCTGGGCTGGGACTAGATGTGCAGACAATC GTAACAGAGACTTTCCGGGTTTTACAACGCGCATTCTCTTGCTGCAATCGCGGACTACGTCACCCCCGTTCATGAGCAGTGCCTAAGGACTAAGGACACC AGCTTTCGTCTACGCGACTTAGTTTGGCACCTTGAAGTCAGCAGCAATATTAGCAAACGCTGCAACTGGCATTAGAGTAAGTGGATACTCGATATTGGCC TCAGGCAGGTCCCATTAGACAAAAGAAGACATAGCACGCCGCCCTGTGGTCCAAACGGTTCCAAGGGGAAAAATGGGAGTGTATCTGCTTTCGGTGGCGG TGCTCTGAACGTACACGTGTAACGTTTAAGTCCTTCCGACCGTGGCGTTTCTCACAATCCTCTTTCGCGGTGCCTTAGCTCCGAATCCCATCGGCAGACA CGCGGGATGAGCCTTGGCCGTTATACCCTAACAGGCCCCCCCTACTATATACCCTTTTCCATTTAACTATTCACCAGCAAGGCACCGAATTACACTAAGA GGGCTGGTTCCTCCCTTGTAGTCTTCGTTGGCCGTGTATCTAGGAGGCATTGCAAGCGAACACGCATATATGAGGGCTGGACGCATTCGCAGCGTTTGCG GTAGGTGTCCTGAGCACATGTTTCGCCACTACGAAACAGCGCGGATTCATACCTTTCACATCAGAACGGAATCCGGCTCCCGTTTTACAGGAACAGGTGC TATGGACTCGGCACAGACTCAGGTCCGCAAGGGCGTGAACCCCTAGTGGTTGAGGTTGTTCATATATACTTGTCCTTTCAATCGTCAAAGCCGAACTATT CTCGGCCCAACAGGCAAGGTGCAACGGTAAGATTTCAAGCGGAAGTATTCAGAAGCATTTACTAATCAAAATGTCTGTCTCTATTCTGGAATCCGGCGTG AGGTATGAACAACTCAGGCGACGCGCGCGGCGTGAGTTAACGATTCCCAAGTATTGCATTGGTACTCGCTCTGGATTGGTTGGTTAGAGACACCCGGGGG TGAAACAAACCTCCCGATCTGTGGTAACACCTTATTACCTGCGGTAGTTCAGATATCATCTCCGCGGGCAGTATGTCTTCTTGGTCTGCCAGATGCCAGT TAAACCCCAGGGTAGCATAGCGGACAGTCGTTGTCGTACGGAAGATGTGGACTAGAGTTTTGCGCGAACCCATCTGCGGGCAGTGCTATTCTCACCGGAT AAGCTTGGAAGATGTTTGGTGGCGACCGCATGACCTGAACATGCTGGGGGCTACACAACGGCGTGCTATCCGCGCCAGAAAGTGTCCCCGGGATCCTAAC TAGCCTTTGAAGAACTAGTCGAGTCCTCTTAATGAGAGGTCGACTCCGGTCCATTAACAGCGCAGCCCCAAACGGTTCACCACTTCCACAGCCCGACACG ATAATTCACCTATAGCGACAGGTTCTGAGCGCTACTAATTGGTAAGCATGTTTATTTACGAATTTTCGAGTAACGGTACGCGTGAGATACGATGTAGAAC CCTTAGCAATCACAGACCGAAGGAGTACCGACTTCCTGGATTGACTAAGGATTTATACAGCAGGAGTCCGATGCGGAAAAACGGGCCAGGCTCCATTTAA TGCGAGCAAAAACCTGTCCCACCTTGAGTCGAGGATTACGAAAGAGTTGCAGCGCAAGCCGTTCACGCTGATATAAAGTCCAGCGCTGAAGACGGTGGGA CGCCCAGGTAGTACAATTCTTGCTGAGAGCTTCGTCCTTGCAACGCATCCGAGTCCAATGATCACATAGTTGCAGTGGGCAAGCGGGCGGTGAGCAAAAC AAACAATGCTATGGGTCTTAACGAGAACAGTCCGCCGTGGTTTTAGTGGCTCTCCTGCAGGTCACCGGTCAGCGTTAAGTACTGGCGTCAGCGTGAATGT ACAGATTCCCTATGGTTGGCGAGTGATGCCCACCGTAGTACTATCGGTTAATGTTAGGAAGAGCGTAGAAGTGCCTACCTTGGATCGGGTATGGAGGGAA AGAGATCAGCAATATCAGGACCACGTGGTACTAGGGTTGGCGCGCGTATAACCCTAATCATTAAAACATCGCAAGGATAGCAAGATGATCTGAACGGGTG TATCACGTATCCCGAGTAGGTCAGTTTTAAGGACCACTGCATTGGACCTTTCGCGATAGGCTCAGGATCGCTAACTGTTATTAGGTGCGTAAAATGCAAG ATCTGGACGTCCTAATGCACGCATGGCGACGACGAACTGGGGGGGGAATCCCCGTTGGGGGCAATTATCTGTGGAAGCCCAGACTTACACCTGTCCCCCA ACGCTTCCTTGGTGGCGGCGGTGGTACACTCGTGAGGTTTGCGTTAACCAAAAGACGGGGCATTTGACCACAGGATTTGGCGCGCCGATTGGGTTGTCCC CAAAATGGATACGCGGCGCGACTGAGTATTGAATGCAAGAAGACCGAGACAGCACCGTCTACATGCAACCCTATAGCATTCTCTGAAGCCACCTAAGGAA TAAGGGCATCATTCATATCTCTAAGTACTGTAACTCAACTGACGCCTGGCCGATAATCGTCTACGTAGCAAATTCGCGCGTGGAGTCATCAACAAAACTC ATAGCTGGGCGCGAAGAACCATTGGGCTAGATGTACGTGCACAATGTTAAAATTAGACGGCTGCATACAGTTACTTATACAAGCTTATTGGCAAGGTAAC GTCAAGTACGAAATGGATATACCGTGTCCCTCTAGTAGAGTGATAAAACCGTACAGTACATTGATCGTCTGTACAACATCCGCACCGAAGTTCGCAATCA TTTGCAGGCGGCTAGATTATAGGCGTCCCTTTGTAGAGGGCTCACCCCTAATTTCCTATTCGAGGCGCCTGGGTGACGGTACAGGGGTTCACACCGTGGG ACCCTACGTTCACTGTAACCGTTGTCCAGTCTTTGACGCTTTAGTTCTGCTGTGCACTCGGTGGAGTGGGTGTCCTATTTCATAGGATATACGCACAGAC AGCTGCGTTCCAAAAACCCCACCAGACCGGGCCACAAACTCGAACATAAAGTCTTGTACTCGCAAGTACTATGATATCAGTGCACACAGCTCGGCTATGA CTCATTCCCGAGCTAGCGAGTCCATTGGTTCAATAGCTGGTTCATACTCCGCATTAACGACTGTACGGTGGCCCTCGGAGCCCGATTGGCCGGGTTGAAG CAGCAATACTTTAGAGCTGTTGGTTCGGCGCCACATGTTTGATTTTATATCCAGGGTGTCGGCGCGAACGGGTGGATGTCATAGTGGTTTCCCATAGTGA GGTCTCGTGTCGTCGCCTTGGGGGCTTGATGTAAGACTATATGCACAGGCATCGACAAATAAGAGGCTCTTAATCGCTAGCGTCCCCCTACTAGTTCGAT TAAAAAGACCCTACTGTATCACAGTCATGCTTGGGACGAGCTGCTCGTCATTAGGGGAAGTGTCTGTGGCCTGTCATTTCGCGGGGCCGAGATCTATGAT GCCTTCTTCAGGGCGAAGCGAAAGCCGCGGGTTGCGAAGGGTATACGCGGGCTCCTGCGACGTTGCAGAGACGGTTTTAGCACCTTCATTGAACGTTACA GATCTTAATCGGGACACAGCCTCCGAACGTAACTCACAAAAATAGCAAGCCGTTCAGAATAGCGCAATGTAACCCCGAGCGTAAGACTTATTGGTCTTGT AGGGCAGCTCCACAAATCAACAGTCGCGGAGTGCGAGTCATGCTGCGCATGTTCGCTAATATGGATACGCAGCATGGGTGGTATAATGGCAAGCTAGCTA CGCACTAAATTTCGGACGCAGGCTTTACGGCTGTGAGATATGTCCAGTCCGAGGGGGTGGCGCTCCTTACGGCCGTTCGTCGCAGTGTCTAGCTTCAGAA ACTGAAGAGTCGTTCACCGAAGGGGAGCGGGTATCCTTGGCTAATTATCCCAAACGCACTATCAGGTGTACTTCGCCAATAAATTTGTAATTGCCCGTTG AGCGTCTGTGGCTCCAACAGGCCCACCAGTGACTCGCATCTCTTCTTGATAGTCGTAGGTTCCAAGTACCGTAGCTACATACCCGAAATACGAGCGCCAT ACTGCAGGGGGGTGGTTCTTGGGGGAGGAAAGTCGTATTGGACGCGGTTCAATTCTCCCCCGTGTGCATGAAACAACGCATTTCTTATGAAACCCTATTG GTACAGTAACTGGGAAGCGGGAGGGCAGAGAGACGACCAGGATAGAGGCGCTTACGCCCTCGTGTATCTGCCCAAGAGCAGGGGAGGGATAGGACGCTGA TTACCTACAAATTATCAGTGTGGCCCGGCTGTGTAGAACTAAGGGTCCGTTCCGCTAAAATTTCCGTCGACGATCGACCCGTGAGGGGAAACGAATGTCC TGCGACATACGAGTGGACAAATGGTCTCTTCCCCTTACAGAATCTATAGTCTACAGAATATCTCATTAGCCTTCCCTTCCTGAAGCATAGTGGTTTCCTC TAGAACTGTCCGCACGAAACAGAATGCGGAGTGTTACTTCAGGCCTGATCCTCCTAATCTTATAGATCGTTCGGCTTGGCTTTACCTCTCCACAATACGA GCCACTGGCTTGCCGAGCTACCGTCGCCGGAGGCAGACGATATTACGTTTCTCAGCGGGCCTCTCTATTATTAGTCCAAACACCTTACGAAGTTATTATT ATGCACATGCTAGCCTCCCTACTCGTAGAAATTGTATGGGTATCCCATTGGCGTTAGGACATCACTAACTGAGCTTCTCAACGTAAACGCATCTCCCCGG GTAAAAAACCACAACTTGAAACGTCCCAGTCGGCGTCAATTGACCTCCCCCCCGACTATCCTCACATGTCTCATAGGCCTATACTGGAGTAGCCTACGTT AGAGTGGAGCATGATGTTTTTTTCCTTAGGGAACTACAGCTCGGCTGACACGCTCTTAGCGTTCGTCGGGTACCGCACCATGGCGCGTCCCCTAACGCTG GTTGTACGGCTTACTCCGTATTTACGAACCTGGCAGCTTCGTCACACATCTCGCATAATCCCTCATCTTCGGTGGTATATCGGCTAACTCAAGTGCCGGG CAGTAAAGGGAGACCTATTGACACTGTGCTATACGAGTACAGAAAGAGGGCTCACTCTGTTATGCGCAAATATGGCCCACTAACTGCCAAAGACTTTAGG GTCTACCTGCCATGGGCCAGCATAGCAAACGCAATTCAGAGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGC TGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCC GAATTCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGG ATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATT TTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAGTAAC GAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATAC ATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTA AGGACTCCCCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTA CCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAG ACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTC GCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAG TGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTT CAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGATTCGCGCCCGCGCCGCGTCCACAGGGGG ACTCGGCGAAAAGTACAAAAGTAGGGGGAGCGCCCTGAGGAGAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCT CTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGA ATCCCCTCGGGTTCGAGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGA TGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTT CTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCCTACCGGAATTCTCATGCACTTCTG CCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGA AACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAAT CCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAG GCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGC CCAGGATATGGAAGTTAAGGCTGGAGGAATAGATAACCGTCGTACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCT GATAATGTTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGC TCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACTCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTA CTGCTGAATGGACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATC CCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACCAATCGACGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCG TTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACGATCGACCTC TAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCG ACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTT GAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAA TGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTC GGGAATCCGTCGCTGGGCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAG TCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTG TGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACA CCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATAC ACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAA CGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCG CGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGA TCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGAGTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGT TAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACC CCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCT GCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACA TGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAAATGATAAACTTGGTGA TAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCT TAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCA AGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACC CCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTT AGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACGA AGGCATGTCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTG TGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTTGTCAGCTGAAGGACTCCTCTGAAGAGTTCTCGCCTGGCCACAGTGCCTTCAATACAGAGTT AGGTGAAGCTTGATCAATGGATCCAGTATGTGACCAAGTAACAAGCTCTGGGACTGCTTTGGCGCTGCGACGAGCTCGGTTGATTTAATGCGACCAACTC TCCAAAGGGAGCAGATATGGTACAGTTAGTGAGTGCAGAGAGAAAGTCCGTGCGGCCCTGGATTTTGGCCTTAACGATGCTAGCGTTTCTTGCGTATCGT GTACGCGGGTCAACGCCGTTTGCCCCTGCGGTTAGGAAGGGGATGAAGATTTATGCTGCAAAAACGGTTAGACGAGCTATCCATGGGCCTAGTAATACCG CGCTTACCCCCTATCTAGTTAAAGTAGAAAGTACGATTTAATGAAATCGACCCTATTTGCACAGGTGATACTGGTTTCAGCCACGAAAGTACAGAGGATA TTTGCCTTGCTGTTTCTAAATCGCGTGAGGATCTAGGACCCTCTCTACGCGTCTCGATTAGGTAGGGGCCACCCCGAGTAGGTTGACCGGGCGACTTTGA TTTATGTAAGACTTGTGGCATCTTTTGATTAGCCAATGACCGCCCCCCCTAGCGGCCCCTCAGGATTTACCTACCTCGTCCATTGATCGAACACTTGATT GTAAACCGTACCACTACGCTCACTAAAAGGCCCAGTGAAGCACCTTCATCCCCGACATAAAAAGTTGAAAATCCCACCAGACTGACCGGACTTTCCAAAC AATAACACGTTCTGGGCTTCGACGGCTGAACAGTGAGGTAATGATAATTGCGTTCGTAGACACTGGCCTGTTGCGTTAAAGCGCTGTGTCCAGAGGGCCG GCCAGCGCTTTTGCGCTCGAGCGAGGTTAGTTATGCTCCTTTTTCGGACTGCGTGGGCAGATCACCTTGAAAGATGCGTGCCGCGGCGCTAAAGTAAATC GTCTGAGACAATCGCCTGGTAGCGGGGTGCAGGCTGAGTCGGAACGCCATAGCAACAATCTTTGCCTTTACTCTCTCTACCAACTCGAGGCGATGGCTAC AACTGCACCAAGCGTGCTAGCCCCACGAAACATCGGCTGAGGATTCGGATGCTGCTAGTATACCAGGGCGGAATTTCCCTGGCATCGTAAGTGGGCTCTC CTTGTATTTCTAAATGTTGGATTGGATAGCTTCAGCAGATAAGCCGGAGCATTTTAGACGGTTTATGGCGGCGGGGTGAGCAGAGTTCAAGTATCGGGCG TTACTAGATTCAAACCGCTTGTTCCGTTACCACGAAACGCACACCGCCAACAGTCCACTCGCAAACAGGAAAGCCCGGCACCCTGCAGGTTTTCTGGGAT AACTTTAGGAACTGCTACATAGACTGTACCTGTTGTTCCTGTCACCGAAAAAGTGCTAGGGACCATTGAGATTGACCCTAGCGGCTGATGCGACTGCGGC GACTCACTGCCGGCTCGTAGGACATGCCGCTCTTTGTAGCAGATTATCCGGTCGCAGGCGCTTCTGTGCTCGTGTCAACTCAAGTAGTTGACTTGTTTGA TAGCACTGACTTGGAAACTCTCCAGTTTTCGGGATGAGTAGGTGACGTGACTCGTGACTGGATGAGGTTTGTAGGGAGCCCCGAAGAGCGTCCTTAACTA AAAGATTAATTATCCTTTCAGAACCTTAAGCTGCGGTTGACTAATCCGCTACAGCTCCCGGCGGGCCCCCGTAGTATCAGGTAGTGGTATGTATAGGTCC CGCTTGACCGCTGTGGATGTGCCACCTTGTCAAAATAGATGAATGTCCAAGGAACGGGATTCAACCCGCTTGTATGGTGCTGCCACTTCTGTGTTCTTCA AGTGGATCGGCCTCGGCTGTTGTTTTAACTGAGTCTAGGTAAGTTAGCATTCGAGGAGTAGATCACCTTCCGAAAAGCACGCTAACTGACCTTCCCAAGG TCTGGATGTGGGCAGGACTATGTCTTGGGTGCATCTGGATCCTTAGTGTAGCATGTCCTTCAGAACGTGTCTTCCAAAGAAAACTGCGCACGATTAATGA ATCGGTGGCCCCTGGAAATCTTAAACGGATGTTGCAGTGCCTTTTATAGATATCTTCGCCGGCGTAAAAGTCGTGCACAGGTCCGGGATTGATCCTGCAA TCAATGCCAACCTCGGCAGCTTCCAATGCGGCGGGCCCGTCACGAAATCCTACACTCAACCGCAGGTCAACGTAGGAGGGTCTTGGACACAAGCACACGT ATGCGAAAATTTACGAGCGCTGACGTCACGACGTAGTCCAACGACAGTCCAAATCATCTGCATATTTTTGCGCTCGGGACTGCCGGGCGCCCAAATAAAC AGTCTTCCTCGGATCATTGGCTTCCAGAGTCCCCTCAGGAGACGTACTCTCTGAGTGAGGCACGCTCTGTGGCCGTTTCACAGCCAATGTTGTACTGGTT ATGTAGCTGGATTGTTTAGTCTAGACTGATGGAGTTTCGACTGCCCATGGTACCTGTGCCCCATAAATGAAAGGGTCATGGAAATGGTGTTGAGGACTAT CCTCCACCCCCTGCGCGCGAACTATTGAAATCTTCACTTGCAAAGAGAACAAGGTTGTGCTGTGAAACACAGCGATATTGATGCAAGGTCTCGAGAGAGA ATAAGGTCGAGATTGGCATGCAGTCACAGCAAACTCTCTTTCGGGAAGTTATTTGTCAGCTACGGCGTGTGTTCTAGCCCCACCTTGTTACCGTCGATAC CTATCTGTGACCCCTACCGGTGCCCGCTTCGATCTCGGGTATCCCTTGCCCTATGGTCGGCATCACGAACGTCGCAGTCGGTTGGAAACCGTCACAATTC GCCGTTCGTAGCAGCCAGGAGAGGAGCGGGGATCTGTCTTTGCTTATAAGTCATGCTACTGGGGGCCGAGCGTCACATGAGAATGCAACTAACTTCGTCC TATGGTCCACTACCAGCTTTGTGTCATCTGGACTAATAGACTAAACTTTGTAATGAAACCCTTGCGCCGGAATTGTTGTGGTTCTAGGTATTAGCACAAG ACCGGCGGATAACTAGCACTTCTCCGCCGTCATAAAAGGAGCTACGGCCTTCAATGCTCCTGTAGGGTATTTGCGGTTCGTCGCGTAAGATTGCAGACAT GACGGCTAGGCGATTTAGTGCAGGATCAAGCATCGAATCATAGGGCGGAACGTGGACGGGTCTGGCGCGGGCGAAGGCGCGTTTTTAGTTACCCAGCGGG TCCTGCGGGAGAATCGAGGTAATAAATTCTTTTACACTTCTCTCTTTACGGTCCGATATCACACGCCGCACCCGTGCTTGGGTTCGTCCCTGCAGTCCCT TCAGGATCCCTTATCACGCTTAGCTCAATGTACTCCAGTCACTCGGCTGACACATCAATCGAGCCCATAGCCGACCAACTCCCAGGAAACTAACTCGGAC CACTACATCGCAGCTGGTTGGCATAACTCGAGCCGTGCGCTTTGACCCAAGGCCGAGAACTGAGTAGATTGGACCGGACGGACGAAGTTATAAGCCGGGA TGAATATACAATGAGGATTTTAAGCACGTACGTTCGCGCGATCTAGATTATAGCGCGTTTGGGCAGAAGATCCCAAAGGCACCAGCTCTCTTCCCCCGGT TCTACACTAGTCATTCGGTGCCGCACTAGTTTACGCGGCAGGTGTTTCAATTTTGCCAGCAAGATATTGGTGATTGCATGACCCCGCACACGTTCATTGA TTGCAACCTAATGGGTGCTAGACAGTGTAACACCCGGCCACGTGCTCCAAACCAACCCCGACATCTCTTAGAAAAACTTATTCTGGCTAGGGTCGTGCCC GAGGGAAGGAGGGATTATCTGTAAAATGGCCGGGGCCACTTTATCACTCATTTTATAGCTCACGACTAGACAAAATGGCGAGCGCCATCGGGGCGGATGC ATATATGTAATGTGTCGAAAGATCTAACGTAACGGCAAACCCAAGAGCGGCACTTGGAAGGAAAAAACGATTAGGATCTCGACATCGCGAATGAGGTAAG AGTGATCCCTGAAGCTGAACGGCCCAGGGTGGAATTAATGTTCCCCCTAAAAACTCGCGCCACAGTACAATTAGCTTAGTGGGTTGGCCCTGCCGACGTC CACGCTTTGAAATGGGGCTAGCAGCCTGAGAAGCCCAATAATAGGTCAGATACGTTGCTATGTTGATAATGCGTACAACTGTTCGCGCGTTCTTCACCAA TAAACTGGCGTAGACAATTCTCAGTTCAGGACTCCGATGTCAGAATCGCCCTCCGAAGGCTATTCGCCTTGTTGGAACACTGGTCGTTCTTAGAACCTTG TATACGTCATTAGACCGATTAAATTGGACGGCCATAGGGCGACTAACAGATTGGGCCAGGCTACAAAGCTGTGGGCCGGAGCGAAAGGTCGTATACAGGT TTCTGTTGATGAGAGTAGGGCACGGCCCTCGTGCTGGACCTTGCTTATAACCCTTCTATCGGTCAGCGTGAATGTATTTGTGACCTATGACTATAGAAAC CCTTAAGACTCGACGGCTCCCAGTACAGGATCGTCAGCCGGACACACTCATGCGTAAGCGAGACGCCTAATCGGGAAAAGATTGAATCTATCGGCTAGCC TCTTCGCCGGCACCGGGCAGTCTAGCGTGCGTGGTAGAAATCTGCGGAGCAACCGTAGGCCGGGGTATTGTGCGCTTTGTACAGCGCGTCTATTGGTCTA TGAGTAGCCCTTTATTTAGACAGGGTTAATTCTCGTCCGATGTGCGCACCTCCCATAACCCGTAATCCGGATAGAATTTCTGCGTCGTCATCGATGATCG CGTGCAGTTGCCTCGATGAAACAACCAGCCGATCCAATCTGCGCATTGCTGAAATGATGTCCCTATTTTGGCATACAGGTAAACAACAACATCTCCAAAA CAAGCTAGGCAACACGTGACCTGCTATCCTTAGGGGACGGGCCCCGCGTTAAAAGGACCGACTTAGTGAGTTTGAAGAATGTGTGTATAATGCCCATGTT CACGATTTTTGCTAAGGAATGTGAGAACGTACATAACGAAGAAAGAACCATTGTTTTCCTAGCTAAAAAGCCGCCATTGACCCAGCGGCAAACTTAGCCG TTGGAGGAGAGGACCGCATATTGTTGTGAAACCATGCCTGTTGTAGTTGTGTTTCATACTTTTGAGTCACTTTTCTATTCAATTGTGGGACCGTAGGCGC CGCTCTCACTCTCTGGGCCCGCAAAATTGGAACGCGTTAACGTCTCGTCCAGAGCCAGAAAATGCGAAAATATGAAGTGTGATTCAACGGCCGCCGAAGC ATAAACTTCCACATCTTCACCCACGTGCTCCGGATTCAGTCCTTGTGAGACGCTAATTGGTAAATCTGATCGATAACTAGTAATAAGAGTGCTTGTCGTT TATTCCGTCTAGCGCCAGGTAACGTTAACTAGCATACCTTTTCTCTTTTCTAGACATGATCACGGAGCCCTAAAGGTGTCTTTGGTAGTAAGATTACGTC AGTATATGGGAGCCTAGAAAACTCTGCCAAAAGGTTAAAGGCGCGTCAACCTTCGGCTCAGTTCGGGTACATCAAGTAGGTAGTACCCCCACTCAGTCTA ACCTATGAATGGTGTGCACGCAGCTTTCCCACGAGACTCAACAGGATAGTAGTAGTGAAGTCACGTTATTGGATATAACGATAGAGCACCTGCTGGCTAC ATTATGTGGTGCGGCAAGAGAAGAGAACTGGGGATTAAAATCGTGCACTTCGATAAGTTCTAGTACCAGACCACAACGAGTTGCGGGGGATTCTCACCGG ACCAGCGCGTTCCGCATCCGGTCTAGGGTAACTTAATAACTTTCGGTTTCATGACAGGTGTAGAGAGGGACCAGTCTTTAATACCGTACCACTCCCCCGG CGGCCGAAACTAGCCGGATATGTCTCATTCCGCTACCATCTCCACAGGCAAAGCCTTCCAATTATTTAGTAGAAGGATAGAGATAACGAGGCGTTCAAGC CGTCATGTTCTCCTATAACTGTATGGCCACGGTCCAAACCTGCTCGCCTAGGTTACTAATGTGCCTGCAGACTTACAGGTAAAGCCCCCATGGCAAGCAG CATAGAAACCTAATCACGACGGAAAGCGGTTCGGACAATGCGTAACTCCATTACATACCTGTATACGGTGTGCCCCAATGGAGGGGTGCGGACATGCCAT GAGACATTCACATGCATTATCTGGTGGGGCGGTTTCACTGAGATCCCTTGTAGCGCCAGATCGTGTTGAGGCGTTGGGGAGGTCACACCTCGCCGCACCG TTACGTCCGTACATGCTTCTAGGCATACAGGCCGGCCCCGCTACCAACACGGTATGTTTCCATCGACAATTAATTCCTACAATGGAAAGGCTTATACGCT AGTGAAATACATGGATCCCCCGCTGTGACGCGCGTGGAGCGAGGTCTAACCTGGTAAACGTGAGTCGGCAGTCACTGCGCACTCCTGCGCGGATTATTCT TAAGCGGCCTGTGATGATAACACCAAGGGCGATCCAACGACAAGTTGCAGGCGGTTACAGTGCCGTGTCTTTCAGTCGACTCCTGCTATGCGGTCCCCAT GGGTTTGTAACCTTTCATAGTTTGTATCTAGCCCGTAAAGCCACGCCTTCAAGATAGCATACCTGCACGTGAATGACACATGGAATCCAATGAAAACCAC GACATACCTAATCACAATATCTGGGTGGGCCCTAGCTATCGCTGGGAGATCTAAGTTGTTATTCTCAGAGCTATTTGTTACCTGCCTATAGGTAAGACCA CTCCGCTGCTACGTGTACCTGTGGCCGAGTCGGCTAAGCTAAACTCCAGGCACAGGCTAATGAGAACTACCCTGGGTACTCTTATAATCCTGTGTATCAA GGCGTTCTATTACTCCAGTTTCGGGCAATCCCACGACGGCCGTTCGTGATGCAATTAGGAGGGCAGCAAACTAAGCTGCCATCCTAGTAGAGGGTGATTA TTAGCAGAAATCTGCTAATGTACCCTACAAGCGTCTAGTGATAGCGGTTGCCGCACGTGTGGTTTTAGGGCTACCTCAAGTCTGCATTCGCCAAACTATG AAAAACAAAATTTCAAAGTGACGACGAGATTTCTTTCTACACGGGTAGGCGCGCCCTAGGGAATCCAATCCTTTTCAGGTACTTCTCGCAAGTGACTCAG TTACCCAACGAAAATATTACGTGGCGAGTGCCAGGGTGCGAGTAGGCATAGCTACCTAACCCATCTACCTGCCACGCTGGCCACAAAAGCAATAGCGTGC TGTCCATACGTGGCTTTCCCGCCGTCGCCTTCGAGTGTAACCGGCCGAGCGTGTCTACCCGCACATGTGGCAGAAATAGAACTATCCAGCGAGTCTCAGC GTCCGCGCGTGGCGATACAAGACCATCGTTATTGCCAGCTTTGTTGCCTATCGATCCATTGTAGGAAGTGGGAGCGCCTCATGTCTACTTCTCCACGTTT CGATCACAGAACCCAGCTGGGTAGAGTTCAGCATGCAGCGCGGTGCTTTACGTTGATGTGAGTTATGACGATTTACCTGTATAAACGGGTGAAGGCCCCA AGTAATGAAGGAACCGGGAACACGGAATTTCCAGTTAAAACCTAACCCTCCACAACTAGCGGATTCCTAACGAGTGCCTTCTGAGTTGCACTCAACCGCG AAAAGTCCCGCTTGAGGACCACGGGTGGGATGCACGACCCGCTGAAGTACCCTTGGACCGGGCACCCTTTTCCTCCCCTTTATGCCTAATAAGACAAGCT GCGTTGTACCTGGTCCCGACTCCCGACGGGCCCGACACCTTTTCTTCAATCTGGGCTTCCCGCGCCCTGTTTCGAACGCCGTCAACCCTCAAGCTTGCAG TTTCACTGATGTGTCTTGACGCTATGTGTTACTTCTACGAGCATCTGAAGTCTTTACGGTACGGGCGTATGTGCCTAAGCCTGGCCTTTCCAATGTGGCC AAGGCTAACTACCGGTTAGCGAGGTCGAACGCACGACTGTGACAAAGCGCCATTAAGTGGTGCAAGGTAGCCGTGGGGGTCTAGCTATTATCGCGTACCA GGCCTTGTAGCGAGCCCTTACCAGACCTCTATCGTGGGGCCTCTTGCCTTCAACTGCCGATGCGGCTATAATAGAATTACTTTGATTAGATAAAGCAACC CGCGCGTGTCAATTGAGATAGCATTCCTTTCGCCTCCACTCTCACATCGATCGATGGTCAGTACCGATACAGATGACAATGCTACACTCTGGCCATCCGC GATCAACACGCACCAATACCATGAGAACCCTACAAAAACCAGACCCTGTTGTCAAACCGGCTTTTTTATATGAGCGTAGGCGTATTTTCAAGCCTCCCCG AGTTTTCAAATCACGAACACTGGTTACATACGCAGCCGAGTTGTGAAATTCCAACTTCTATGTCATGTCTACGAACAAGTGTCGCTGAATAAACACCGGG ACCTATAAAGGTACAGACGTGTGTCAGTCGACCCGACCTCTTACTACAAAGCTCCATAATATTGCTGTAACCACTTCACAATTACGTCATTCTCGCGCGG GACGCGCTTATACGTCGGTGAGGTCGTGGCGCTATTAGCGACTGTTCGTGTCAAGCAGCGTCCACCCGGCCTCTCATAATCTGCAGCCGTGCAGCCGACA TAGGACACTATTCCCGAGATGGTGGCCTCGAGAGCCGGGCCCGGGGTCAGTCAAGGTTAGTAAGGACACGGGAGGAACTCTTCCAATACTCTCCTTCTCG TTTCGGGGCTTACCAGCTAAGTCGGCTTAGCTAAGTTCAATTCCATGGCTCATGTTGAACCGCAGTTCTGTAGGAGGTTGAGTTAGCCAGTTGACCATCT AAAAAACTTTGTGCGGTCACTAACCCGACTTAACAGCGGGCACCGTAGCTTTATGCGGTGGCCACACCTGTAGCTATTGAACAACTGTGTATCAAAAGCG CGGGGGCGTCATTTATCAACACTACTAATGAACCTATCACAGTCAGTTACAGGAAATTTATATTCAGGACTCCGACGTGCGGATCGTGGAGGGTGTCGTT CTAGGTCGTGTTTGTCGTAGGGGATTGACTCTATCGGTCTTAGTTATCTTTGGGCGAAGAATAAGGGGGTCCGGGCGTTTGACCGTTCACGCGTCCGCGC TCATGGCCAGGCTGATTAGGAGGACATTTCAACCATGGGAGATAGAAGGCTACTCGTCAGGCCGAGCAGAGGCAGGCGGACTTAGCCTTCTTGAAAGTTT ATCTGGCATGGATTAGTAAGTTGTCCGTTAAGCACCCGCCTCGTTTTATGGGAGCCCCTGGCAGAGAGATTAAGCGGTCATTCTAACCAACCCCAGACAG CTCTTTTTTGCTAGCCGAGATTTGGCATCGATAGGGATACATCGTGTCTTACCCGACGAGTTATACCGTGGTCTTCGTTATCAAACATCAAAGTCCCGCC CAGGGAACGTAGCTTTTTCGCACCTGCTTATTGCCAAGGGCGTGATCTCCGCGCAAGCTCCTGACGTACAAGGTAATTTTACGTGGGGAACCTCTCGTCG AAGACGGTTACAATGGTGACCGACCAGCACGAGCATTAAGGGAATGCCATGTACCAATCATATGGGATCAACTAGGACTTTTCAAGACCTTCCCCTGCAA GTCAAGAAAACTGACTCTCCAAGCCAGTAGGTCCTTGACCCTGGTGTACGGATATGGCAACTGCCCTAGCTGTTCAGAAGGCCTGATGATAGATCATTTA CCAGATAATGTCTCTGGTAAGTGCCGCTCATAAGAGATAGACCTGACGTTAAGAAGAGATACACGCCCGGCGGGCGTTAATTGGTAGGACTGAGAGACAC CCTGGGAACAGAGCGCAAAAAATCTGTGCTACAGTACGTAGATGTTTCCCAAGAACCATGTGAAGAGAAGACGGGGAAAGCGTAATCTCGCAATTGAGTC CTGAATCAGTCCCTGCGTGTGAAGATAACTGTACACTACGCGACGAATCTGAACCCTCGTCTGTTGGCTCCATACGCAAGGAAATTATGCACGAGCTATC GTCAAGATATGACAACTTTAAGCTACGCGAGTCCCCTAGATCCGCGAAATACAGTACTTCCCCGATTATATGCCTATGCCAATCGGACGAACGGCCCCTT AGTTAGCACGTGTAGACACTTCTCCCCAGAACCTAAAGAAGGCGTCGGGAGCGTTGAACGAAGAGATCGGATGTCCTCGGGTAGCTCGCTTAGACCTTAG TGATGGTACTAAGTGGCTATGCGCGCTCTCAGCGTTAACGAAAATAACGACTATGATGAAGTCAAAAGACACTAGCCTGAAGATTCGCGGGGGGCAAGCT CCTTTCTGTCCCACAATTACCACGGGCGAAAGATCGTATCTAGCCTAAACTATCGGACCATACGTCTCAGTGCTGGACAAGACGAACGAGAACCTGCTGT TCCATCTGCGACCGGTCGTTGGCAAACGCAGACTAACCCATACGCGCTTGATGAACATTGGGACTGTGGCTTTAAAGGGAGTGGAGTACCTAACGCCATC ACTGCTTGACTAAAGTCATTAGACCAGCGTCTCTACCCGACCAGGGTTCGGCGGCGACGGCGATAGAAACTTCTGCGTATGGGTATCCAGCTCTGGAGAT ATAGTGCTGATCCATACGGCAGGTTGTGTAAACCCCCTCTAAAGCGTAATGGTCGCAAGAAGGACATGCGCAACCGAGTCCACCTGCTCCGTAATGTTTG AGAGAGACGTCATTGTCTCTGCTACAACTACATCGCTCTCACGATCGATCTATTGATTTAGACCTTTGTTGAGGAATAATTCAATCTGCAGGATACGGGG CAATCCAGGAAAGGAGGGCGATTTTGCTCAACACTTAAGAACGATCACGCAGTACTAACCAGCGGAAGCGTTGGTTACTGACTTCCGATTCTACAACCAC GGGCCACTCGGTGTATACGGAAGTACGTTTGGTGAGGGTCGGCTCATCTAGGTCTGTGCATTCTGGAGAAATGCCAAACGTCACGTCTTCATCAACCCGG GCCGATAATAGTCTTATTTACGTCCGCCGGTTTCTCAACACTCAGTTACAACAGGTCGTTTTGCGCGAGGTAAAGACCCAAAATTGAGTACGCACCAAAA CCGATCAGGAGCACTTGTCGCCGAAATGATCGGGTTCTAGCCTCGTCGTATGGTGGTAAACTTCAGACGTACAAGGTGAGTGTTTGGCGCCCAATCTAGC TGGCGGCGGACTCGACGTGTTTATTCCAAAGAAGGACGTTCGCCATTGTGAGAGTTTATATTCGCGCTGAACCATAGCTTTTGGGGGTGTTGAAAAGATC ATAGTACATACCGAGATCGCGCGACACGATGGGGGCAGTTACTCTTTACAAAAGAGTGTTACCGTTCCTAAGGGTATGTTGTTGGAGCCGAATTAGGGTA CGCAACCGCCAGGGCGGTGGGCGGCACTAAAACTGAACGTTTCTCGAACCCGTACACGCCGGATGTTGTCTTCGCGACAAGACGGTAGAATTAATGCCGT TACAGGAGTGGGTGAATTTTATGAATCCAAAAACGGCCAAACAGTCAACGCGTCTACGTCCGACAGTCGGTATAGTACTAAATGAACTGACACGTCTCTG ACAATAGACACCAAAGAATCGGCCATGTGCGCCGTACGGGACTGTGGTTTTGATTGCGAACTGCGTTAGTAACAGCCAGAGCGCACTTGGCAGAGCGTCT CGCACATTAAAAAACTAAAACGCTGGGCACTTAATTGGAATGAAGCTGGGCGCGCCGGATGGCGGCGCAAATAAAAATATACCATTGGGGAGTGAGCAGT TAAAAGCGTGAGTTACTGAAGTGCAGCGAGGAAGGCGTTAGGAGGTTCAATGCCTACCCAAGTTACAGAAATTGTTCTGTTACGTACCCCTGGTTGTATT ACGCATGAGGCAGCCGGTGATGCGTCGTCACATATTATCCTCCGCTCCGCTACTTGCGCTGTTAAGACCTCGTGCTCATCATGACCGCGAGGACATCCCC AACTCTCAGTTGGCAGGTTCTTGCAATCAACCGACTACGTGAGCTACCTCGAACCCTGGAACATAGAGACTAGAGCTTAACCCAACACCCGCAGTATGGT TAAGCCACCCAGAGTCACGGATGACCTGACTGTGGGTATCAGCATCACACCCAATACATCTCTGTTTCCTATTGAGCTCTTCGCTGGGTGGAACTTATAC CGTTTAGGAATCTGCCGAGCTTTGGCCGTGAAATTCGGGCCAGCGTGGCGTTCATGCTACCAAGAAATTGTGATATGCAGGACAGAATAACTCTAATTGG TTTAATGTCTATATTTTAGTACCCACGCCCCGTGGTCGATACTGCACAGACTAGTTTCTAAATCGGGAAGAGCGAATTACGAACAGCCATAGCCAAATCA CAACTACTGTTGCTACATGACAACCTTTTACGCGAAGCCCTGACGACAATCGGTTAGCATGGATGCTAAGAGGAAGGACGAGCCCCGGGTATTCAATTGG GAGAAACATTACTGTGTTCGGCTAGAAGCTCGTTCCACTGCCCCCGGGTCCTGGAGTAAGGTATATGCATGGATAGAATGGGTGGGACGAAGTAGGAACA ATTATGAATTCCACGAACGAGTTTTTACAATACACCGTCACGGAGTATTTGGCCCATTCGGCTATGGTAGACGTTTGTGTTAGGTCAGTCCAGATTGTTC ACAAAACTCTTCGAGACGACTTGAATTGCCCGTCCCATCTGGGGAATAGGCGAATGTAACCGCACGCGGAGACCTCCGGGAGACTTCGGGGACACTGGGC GATTGCACCGGAGTAACTTGGTCTAATCTGACTTCCCTTGCCGGGCACACGGACTGCGCAATGCACTATAACAGCATTTATTAGGCGTTTCAAGGCTAGA TGGCCTCTGGTGATATTCGTGATATTCTTCTAACATTTCAGTTAGGTTGTCTGTATATAAAAAAAGGGTCAAGATAATCGGGAATAGCATGCGTGGTATC TTGCGAGCTCCCCCCTTATTTTCTATAGGAGATTGAGAGGAGGCGAGTAACCTAACATACGAGGGGGTAGTCAATAGAAAACTGTTCACAGATTTCGTAT TAGACTCCATGTTTCAATGGCTTCAGTGAGTAAGCGCAGCTACTCGAGGCCCCGTGTGCCAGGAGTACTTGATCGGGAACCTCATGCCCTACGCCGCCAG TCGTTAGTCCGGCTCAAGTCTCTGGGTAAGTTAGCAGCATTCAATGTTAAGTCTTAGTTTTACCGTATTGGGTTTATATCCTAAGTCAATGCTGTATAAG TCGGCCCCTTCGGAGTATCATGCTTTGCGTTAGTCCGATTCGTGTCCCTACGCGGATCTTTGTATGGGCTTTGCTCAGTCAGGGTGATTTCCGCTGCTAG CGGGTCATATCTTCAATTGTAATATAAGGTGAGCGTACTGCTTCCCTACCACAAACTCACACTTCGGCACGGAGCCGTCAGCAAAAGTTTAGTAGGCGTA GGACCTCATCGTCGTTGTACGGTAGTTCAATGACGCAAGGTGTGAATTGCTACAGGTGCGAGAGGAGGCGTGTTATTGCTGCTGCGAAGGGTAATATGAA GTCGCCTTTTCAGGGGTTAATTAGGGCTTCTTGAGCACTGATGGCGGTATGTTGATTATAGATCGTTACACGCGGGGTCCTTATAATTGCACCCCACGGT GTGATCCGCTAGATGTCTATCGCCTTATCTTTTAGTATAGGTGTCACAAGCGTGTGCCTCCATGGCCAAAACATGGCTAGCGAATCTGGCTTTAGTTCGC CGAAGTTTTAGCAGACAACAAGCAGCTGTAGGCGGGCGTTAGTGCTGGTAATGAGCGGCGGGAAAACCTGTCCGGCGGCTATGAGCGGGTTGTCTCTTAG AGTCCTGCACTATTACGCCGGGTTGTAACTAGTAATTTGAGAAACTTTTCTATATTTGGGAGCCCTTGCTCAAGGCATACGCCTTTGCCTAGTTAACAAC TTAATGTATGTTTTGCAATCGAGTGAAAGCGACTCTCACAGTGGCAGGAAGAAAAGTTCGAGGTTGTTCTCAGATCCCGCTAGCTTCTTTGTGATGCTCC ACTCACGAGCTGCCCATCGGGCTGGAAGTTCTCCATTGCGGAGCCGCACTACCGGAAACAATTGCCCCAGATCATCTGTTGGTCCTATGGTAAAAGTAAT GATAAAGGATGATAAGATTCAGCGAGGCTGCTGTAAGACATGGCTCCTGGGATTACGTGATTCCGCGAAGATTCTGTAGACGGTTAGAACAGGAAGCATG TGTGGCAGGCCGTGCTACAGTCTCATCTGCGTTCCGTTAGCAGCCGCATCTGATTTGTGAACGAGGTGCGAATCTGACCAGTAGCTCAGTTACCAATGTA ACGATCCTAGGAGCTTCGATAAGTTGTCGCTAAGCCATTTTCGAACTCACGACCTTCGGTTATTATTCCCCGAAACCAAAACTACATATTTATCATTTGA AATTTGCGTATCCTCCCGCTCTAAGGTCGAGCGAATTGTATTGCAGTATTGCTCCCCCGCAAAAGGCGTCCAGGGTATTGCGAGGACGCGTTCGCCTACT CCGGCCCAGATGAAAAACCTGTGAGTATTTCACTTATGTACATGTAGACTCGAGTACAACAGCGTATACCGTCTCATTTATCGGGGCGGCTAGACGGCGC GCGGAAACCTGCACCAGGTCAGTGGTTCCAGGGACGGGTCTGCACACCAATGGAAGAACAAATCGAGAGCTCTCACCCATGCTCTGTATAATGAGTCAAA CCTCGGAAGGACTGGTTTTCGTTTTCAGCGAGTCCTTCGTTCGTTATTCAGTGCCAACCTTCGAGGACTAGCGAGTCCATCTCAAGTTGTATACGACTTT CGTTGACACACAGTCGGCTAAAACATATCCGGTGCTTGCCACCAAGTCACGACTTAACTGTGCCGATGACATATTGTACCTTGTGATGCGGTGAAACTAT GATCCGTCCTCGAGCGCTGAGGATGGTGTAGGCGGGCCCCTAAGAACTTCCTCACACTACACTGTTCGCGTGCACCGGTGCTGACTCATACCGCATCCCC ATGACCTCCGTGTTTTTTATCGTTTCACGGTTGGCTAGAATGGACTACCAACTAGATAATTTGTCTGACAGTAGATAGGCAAGAGGCGTTCAAAACAAAC ATTGGATATCGTAACGTGTCTCTACAATCTGATATACGTGCCGACAGCTCACTAAACTGCGTCATATGAACAGGACGGCAGCTGTTGACGAGGGGACGGG TCGAGAAGATGCCACCAAGTGTATGCTCTACACGTTAGTGTCTGATAGTATAAACTTACGATCAATACATACTGATATTCTTACGACTGGATACCAAGTG TTTAGTTTCCAGGCCTTAGCCACTTACGTAGATATCCTTAGTTTAGTCCATGCGCACCCGGTGTTCCGGAATGTTCCCGTATCTTTACAGTCACCACTGC GCATGAGGAGAGATTAGTCACTTATCAGTATAGTACTGACCTAAGCGCCTGTGACTCTCGCCCACTGTTGATTTAGGCCATTGGACTGGGGCGGACTCGT CGATGATCCGACAGCTGCTACTCTTACCCCCTGGGAATTTGACCAGTCTGTGTAAGGGACCTGTTGGTAGTCAATTATCCATTGGTGGTTGCTTTCGCGG CCACTCTTATTAATGATACGCTTGCATCGCCTTGACTCCCTCTCACTCTAACATTCGCCTTTCCGAGTCCGACCTTGGCCATGCATTGGACCCGGCCTGG TGGCGTAGCAACGGCACTTATTGATAAATCTCTTCTCCTTGCGGAATTGAGTATAGCTGAACTGCATCCACTCCTATTGCGGTACTCGAGATGCATTGGG CCACAATGTACCATGTGTTAATACTGGGGGAACTTATTCTTTCCGCTTATGTCTTACAAGGGCGTGCCATCACATGTGGGGCATACGGAAATTATAGGTC TGGTGCCTGTGTATATAAGTGTAAGAAATTATCATTGAAGAAATGCCCGGACGCAGTGCAGCAGGTTCTAATTCCATATATTTATTATAAGCGGATGAGT CCCCCAGCCTGCTGCGGAGTCTTGTTGGCGTGAGCACTTCTTATTTCAATGACGACTCTAGAAACAAGTATACATCATTGCACTTATAAATCTTCAGACC CGGCAACTAGTTCAATCCGTCATTGATCTAGCAGGCTGCCTAATCGAACTGCGCTCACGTCGCCCACCATCGACCTCAAAGGGAGTCAAGCTGTAGGATG TCTGTTGCTGCGGCGGTGGCTACCCGTAGATCACCGATTTCATCGACGCACCGTTGAAAGTCCCTCATTAGTATACAAAGTATATTAAGGAGTTGTAAGG TCTGTCTGTCCGTCCAGAACTACCACGCGACGATCGAAAATCGGGCTATTCGACATCAACTTCCTACCGCGCGCCGATTCCTAATCTGAAGATCAAGGAA GGATCGGTAATGCGCAGGCCAGCGAAGCTCTCTTTTCAGGTTATCACTATCTACCCGACGTATTAATGTTCGCCGTTGATCCGATCAACTTCTGGTATAC TTCAACGGGGGCCGGTCATAACAACATAGTCAAGTAGGCTTCCCGTAGTTAGCAGCTTACGGCAGTCCCAAGACGCGAGCACTCATCTTAAGTTCGTTAT GTAGAGCCTATCCTGATCCGCTCCGGTATGGGTAGAGGTATATTGACTCGGCTGTGTTGACGCTTTGATCCTTCTCGATATCTATCAGACAGCCCAGCTG TAGTCACCATCACACTGCACCACGTAAATGGCAAGGTGTAGCAGGGGTTCTGATATTTTGGCACGTAAATATGCGATTCCGGTGGGTTCAGATTGAATCC GACGATTAACATGAGATGCTTCATAAGAGCTCGACGTAAACCGCGCTCTGTAGGTCAGACTTCTGACGCTCAATACGCGTATGTTCAAGCACCCCGCCCG ACCCTTTGTGGTGTCCCTCACAGGCGTGTTTCTGAGCAGTAGTCCGGCTCGCTTGCATGCCTCGAGTCTTAGAAGACTCTCTCGTATGTTAGACGTGTCC AGATATGGACTCTCGTGTAGTCCTGGTTGGGTGGTGGCATATACACCCTACGAGCTTGTTGGGCTCGCGTATAACCCCAAGGGTGAAAATCAGGAACAAC CATATACCATAGCGGGCTGCGAGAAAGCTGTTTTGCCGGAAATACAGTAGTAAGTTCGTTTAGCCCGTCGTACGAGCGCGATCTTAGGGCATTAGGGGGT GTAAGCGGAAAGCTGTCCACCCATGTACCTCATAGGACTATCAGCTGTCAATGATTCGGCGTTTGGGACAACAAAAACACGCGCCCAAGTCTAGGCCATA CCGCTATAACTCATTCTTCGACGCTCACAGGGTTCGTAAAAGCGGGAGAGGCTTACTACTTGGCTTTAGGTCGTATACCGTAATGAGCCTACAATGATTT ACACACGATTATGATTCTCGCCGCACGATGGTTGCAGACATAAACTCCCGAAAACCATTCGTAAACTACCGCTGGCTTCTGGCCTGAGCCATTCGTCGCG ACTATGCTCAGCTAATGTGATATGGCTCCACCAGCATGGAAATAGATCGTCTACAAAATGACGTGGCTGCAACTGTATTTGAGATCGTAAGGGAACGTAC GCGCTTAGCTAGGAGGCAACGACCTCTTCCCTGGTGAAGGCTCTGGCATCGGACCCCACAAAGCTTCTTTCTGCCCCACGCTATGAAGCTGCCCTCGTCT TATTGCATAGTCCCGTGCAGGCAGTCCAACGTCGCTGAGTTTGTGCGTAACTATTACCGCCTGCGCTGCAGTTACGCACACTGGGAGCTATGGTGCTACG ATCCGTGCGTACCCAGAGGACTCGTTGTCCTCGACCCTAAATTCGAACGCCATATACCTGTTAGTTCGAGGTCGTTGGCGGATCTCGTGAGTCCTTAAGG TTGTAATAAAAAGTGACTACCGTTGTTGCTTAACTGGTTCTTCCAATTGACTAGGGGCGTCTTGTGTAAGATAATGACCTTCACCGCACCTGGCTCGGTA CACCCATGACCTCACTTACCTACTACGTAGCCGATCTCTACGTACCATTCTCGTGAGCCCGCAGTTCTGAGCCCCTCTCCTCACTGAAGTCGTTCCTACT TTGAGAGTACTGTCTATCTAACGGCCGCATCCGTAAGCCGACTAAACATCGGGGGTTGTATGTGGTTCAGGAACATAGCAACCAGAGATACTTTGCTTGT GCCGGTTGACTTATAACGATCCATTTCATATCAGATTACCTGGCGTCGAAATCTAAATATCGAATCCTTCGTAGCCCAAGTCTAGCGAAACTCCAGTGAC CAAGGACGTATTACGACAAAATAGGTGGTACATTAGGATCCTTGAACAGGCGAGAACGCCAAAGGCATACTGATGGCTAAATGACCTTGACCATATGCCG CGTTGTCGAATGCGAGTGGAAAACACCCAGGCCTAGCTACGGAGACCTTAAAAACCAAAATATTATAGGACGCATGGTTGCTCCCCGCCGAGTTGCGCTT CACCGCGTCACTTTGTTGATATATTTGAATGAATTCTCTGACTGCCAGAAAGACTCATGCACTGCTCTTTAGTAGTGCAGGCCGAGAAAATTCCGAGCGA ACCCTGTCCGCGCCTTCCTGACACATCTTTCCTTCCGGAGTGGGCTTAGTATGGCTTCCGAATAGCCGAGCTACGTTGTTTTGATAGGAAAACCGCCAGT TGTTTATAAAAATGGGGTGCCCTATGGACGTCCGATTTGGTATCACTGACTATCTCCGCTTCACTGTACGGGGGATACACCAGAGTAACTGGGCAATAAG TGCCAAAGATTAGTTGCGAACGACTCACGTTCATGGTCAATTCGTTATCCTTAAAGTTATTGCCTGCTACCATAGCTTATCAGAACGAGCGGCCCTTTGA GTCGCCCGTGAGAAGACCGATTCCAGACTAGCAATATAGATTTGTGGGGTTAAAAGGCTGCCTCCGTGTAGTCTTTCAAAAAAGCACGTAAATCTGTAGT GGGCCTTTGGATATTGTAGGGGGAAAAGCGGAGACATTCAAGAGGCAGTTTTTTGCGTTTGTATGGCAGGCCATTTTAGAGCCTTTAAGTGGCACATAAC CGCGGAAGCTTGGAATGGAAACGGGATCTAAGTCGCATTCAATTTCTTGATGAGTCTGGCGTTCGGTGGCGTGGCTCTTAGGTGAGAACGACTGACATAC CCTGCTGCTGAATCCGTGAGAAACCGAACTTGGCGCAATCTTTAGCAGGCGTACACATGGGCGCGAGACGTGCGGTAGCGAGCAAGAGCTGCTAAGTGAG TCTCCTCCAGTGTCTTCGAGCGCCTGGAAGTTCGGCCCCCCTACGGAGTCGCGCTCTCGCAGATTGCAGAGCACAGTATTCACCACTACTGTTCTTATTT CTTGCACACAGTGCCATCACGCTCACTTCTATAGAGGCTACGCCGGAACGTCCATTCGCAGGAAATCTCTGGCATGCTCCGGTGAGGCAGGTGGTCCCAG GGGCTGCTTTGCATAACTACCGGCTCCCAGCGACGCACGCACTCTGAAACAGTTCCCCTCTTTCAACCTGTTTCTACAGTAGTGCGCTGTGCACTTTCTC GTAGGTACCCCTTCCTTATCACGGAAAGGAATCGGACGAAAACTCGTTCTGAAGCGCTGGGACTTCCCCGGGGGCACACAGCCATTGGAGTATCGACGAT GGCCGTCCAGAGTCTTAAAACTATAGTTGAGTCCAGGACGGTGGACTCAAATACGCAACGATGAGGACTACCCGATTGAGTTAAAGTAAACGTTGAAATT GTTTGTGGTGCGTGGACGATTCTCCTCAGCGGACACGTTAACGGGCCATGGTCCTTGCTGCGGTTTTCGTCGGGGTCCAAGCGCTACGGCCGATTTTTGG GGGCCAAAGGTAGCGATGTTTAAAGCCGAGGTTAACGCTCTGTGGAGACTGGACGCTGTAACTTAGCATCGGGGCCTGATCCCGGGTGTGCAGGACCCCC TGCGTCTGCGTTCACAGACCTGTCGAGGGTGGAGACTTATTCCGAAAGTTTCGATATCAAGATGATGGAGGGCCAGAACCTTCCACCACCAGATGAGTTG AGGCTGAACGCGGCCAGTCTTTGGTATGTCATGACTTCTTACTTTTTACCTATTCTTGGCCTCACCTCTTGGCCATCTCCACATGAGATCCTTTGCAAAT CGTGAGGCGAGAAATCCTAAAGGGGCGTGATGCAGAGGCGCAAGCACTTGTTCGCGTCTTGCTCCAATCTGACTAGTCGTTGAATCGATTTAGATGCCGA TCCTCACGAATTCAAGAGATCTATCTTTGTTGAGTATATCGCAGTATGTTTCATGGAACCGGTGTCGGCTTTAGAGTGCAAAATCTCCAGCTTCTTCACC TATGTCGAAGGCAGGATGGTCTCATACCCGAAGCGTATTTGAAAACGCCTTTCCGATCAAAAAGAAGAGCACTCACACGGTGAATTCCACCCCTTGATCA CTACGAGTCGGTCCCGCCCTTATCAATACGCAATCTCTTACCGTCGTACGGTACTCGAGGCCCACCTCGGCGTTTCAGGCTACCTTTGTCCCTATCTCGC TAGGCGCTTCGGCCGCGCGTCGCGTTGTACGCCCTTCCATGCGTCAGTATAGGAACGGGGTCAGCCGGCTTTACTTCTAATCTGGGGAGGAAAAATGAAG ACATGCGGTAGAACAAAAAGGTTGGCTGATCATTGCCATAAGTGGTGTAAGATCGAGGGAATGCCCGGAGTAAAAGTGCTGCGCGGTTGGATCGCAGGCC ATGGACCCGCCGCTGGCTCCCTACAGTAAATAAAGCCTTAGCGTAGAGTGGATTTTCGCTTCTACCGTCTGCGTCGGGATGTCAGGCGCCACTGGGATCT TACGTTGTGGCTTTAGAACGGGAGCAATAAAACATTCACCATCGGAAAGAGCGGGCCAGTGAGCTAATAGATGTTATATCTATATAAGTACCTTGAATTG AGCGGACAGCCTGTACGGGGAATGAGGTTCCGCTAAGATACGGCCCACGCGGTAAGCTTCAACTGATAACAGCAGACGTGCTCGCATTTCTATTTCTATC CAGTGACTAGACGAGCAACATTTGCGGAATTTCTGCTCTAATCCCTATAGGACCACTAGTTACATAATTGGTGGACGCGAACCGGCCCCAGCGAAGCACG CCGCTTCTGTTTTACTCTACGCGAGTTGCAGTGACTTTGCCGCCATCCCTCGAATTTAGTTGAACATACGGGGGCCCCCCTATCCGCTCAGGAATTTCCC CAGGTCCTCTCCGAATACATAATGCACTCATTAGGTACCTTGAAATTGATCCCTTCCTGCATTGATAGAGAGCCGTCCGGGCCTTCCTTCTAACGAATCC AGAGATGCGGTTGCGGTTATAAATTATACGCTCCGCTTAGACGCCAGACAGCCCCGGACCTAGCATCACGGGATCCCAGGAATCGACGGGTATGGTCATA AGGTTCTGCACCAATCGGTAGGTTGGGTTCCGCTCGCGGTGCAATTAATGTGGAGCTTGATTGCGGGTTGGGTATTTTTCACCAGGCAGTTTCTATGTCT AAGATATTTGAGTGACTCTCGATGGTCTCGCATTTCCCACTAAGATCTTCGGGTTCCTTCTAACGCACTTGTGCGACTGAGACCAGCTCTGGGGTAAGGC GGACGTCGAGACAAAATTGCCCATTCAGGGGACGGCAATGTAGCCGCCAGGTTTCCGGTAGATATGGCTAGGGCTCGTGCATAATGATGGTCTTGAACTG TTTACGACCCCCTTTATTAAGGTCTGCTCTGATCAGCGACCGCCTAGTGAATCTATTTTGACCCGGCTCGGATGCAGATCCGTGGCTTCTTTTTTAAGCG CAAGAGCGGTAAGACAATAAAGTTATAGATTCGGTTGACCTGCTTCCTCTGGTATGGACATCAAATTGCCTTTTGAGGGCTCAGTTTCACTAACCTCAAC CACCCCGAGAAACGTGGCCAGGACAAGCGTCCTGAGACGTGAACGGACTGCGTCAGCCCCGAGGCCCCCAGTAATGAGTTCAATTGGGCCAGACGAGGCC TACGGTCCAGCCCGTACTCAAGCATTTTGTCATTCTGTCCGTTCGAACCTGTGACGCCTCATCAACTTTCTACGTAACCCGCCTTCGAGTTAACTAGGAT CCGGCAGCCTAGAATGCTAGGCCGGCCGCCCACAACAGTACCGGCGGTTTCCGTTGATGCGTGCGGGTAACACAACACTGCTTGGCCATTCCTGTGTCAT GAGCATTTTTTACACACATGGCTTGTAGTTGGACGCTAGCCCACTGAAGAACGCTGAAAAGTGTGTTGATCCTACCTGGTGCAAAAGGATAAGCAAGATT CCCATGTTATCCGGCCACATAGTCTGTTGGTAAGAGTGCTCCTTGCGCAGGAACCCGCCTAAATCCTTTGCGGCTAGCCTTGTATCTGTAATCCTGCAGT TGAGTATTTTCCAAGGAGCTAACCATGCAGAAATCACCATGACATGGGCATCGAGTAAGAAAGGCTAGCTTGCAGACTGTACTAGAATGTCGCCTTTTAA GGAGGTGAAGCGTCGGCTTAACGGTGTACGTGTGTTGCCCGATAATGATTGCGGCAGGGGAGAGGGACATGGTTAGGTCTCTGATACTTTTAGTGAAAAG TCCGCGCACACCTAGTAAGCGCTGATGACTTGATTTCGTCGTGAATCATCAACAACGCACCGCGCCGAGAGCTGGTTGCATGTGAGCGCACGTATCGCTG CCATGTACGGCATCCGATGGTCAATTGATATCAGCGCGCAGGCCTTTTATCGCGACGGCATTCACTCGGATATCGATCTGGATCCAGCTAGCGCTTATGG CTACAATACCACACCGCTTGCATATTTTTATACCTTTTCGGAGTCGTTTGCCAAAGCTCAACTCAGCCCTACGCCTTTTGTTCCGTAGCCTCACTGGGCT ACCCCGAGGTCTACGACACCTACCTCTTAAACCGGAAGCAACCCGATACCGAGATGCGTGCCGAAGGCCCGTCACCCGGTTTGCATCCGGGCTACTTAAG ACAAATCCTCCCGTGCTGCCTCTCAAAGGGGATCTCGTATTTCGCCGACGCACGGTGCGGCTCAGTGCAAGCTACGAGTGAAATCGTGTCATACCGTTTG GTGATTAAAGCTCCCGATGTTGGGGTCACCTGTCGGCTCTTTGTCTACGGTGTACGGACTTGTTTTTGTAGGAGGTGTCTACCACTCGAAGCAAAACGTA TCTGCTCAATCAACGCATGTGTGTTTCTATGGCTCGGTTGGTTGCTATGACGAGCAAGATGTGGCCCGTACTGACCCACATGCTTAAAAAACACCAGGTA TGTCTCGGGACTAACGTCTACAGTCTGACCGATTCCGCTTAAACCATCCACATATGGTTCAGAGTATATGTAACCCGCGCTGTCACGGCGCTCGATGAGG TCTGATTTCCTAGCGGTTCTGGGATGCACCTGGCAATAACTCGGATGTCCTACTATTTAGACACTCACCGTTTTAACTAGGATTAATTTACTAGTTCGTC GACTGGATGCCGCACACCGACCGGCGTTCCTGCTATGGACACGGCTATACTTTGCGCACTGGCGTGCCCCACCAGCTCTACCCATATGGTTGGAACACTA AGGAATCAATAGAGGCTGCCGCTCCACACGATCCAAAAATGACCTTCAGCCTCGAGAGTAAGCGCCCGGCACTTCTGAAGCTAGAACAACACAAACACGC CGCGTCGCTAGGTTGTGTATGCAATGTGTTCTCATCGCTCTCCAGCCGTAATAGGCGTGCGGGCTTTTTGCTACGATGGCGTCGAAGATTCTCCTCGCTA CCGATCGATCCCGCTGCCACGGAAGCCCTGCCCTGTTAGTGGCATGGTAACCATTGATCTCCTTCTCGGCCGTCACTTATCAAGGGTTTTCATGACGCGC ATAGCATTACTGAACTACGCTACACCCAACGTGCAGTACTCCGTTTCACATGGGTCGCGTTAGAGAGACCTTAATCGGCATATAACAACATCCAGACCTA CGTAAAGCTCCCACCGCGCTAAATGAGAGGCCTAGGTTCCCGATGCTGTTCAAGTCTGGACCCTACTCGCCCCACGGCCTTATCAAGGATTCCATCAGTC CGGTTTAACGCTATAATCTGTTCAAGCTAGAACAAAGGGGCGGGCGAAGCATACCTCGCCAAGTTACGTTTGATTATCAGAGCATCTGCACAACTTTAAA GTTTTAGAACACCCGAGACTGGTATCAGATCAGTATGAAGGTGGAAACTTCTAGTACCCATAAAAGATGGACAACTCGTCTTACATAGCTACGGCGCCCG CTTAAAATAACGTATTACACTAAGGTAGGCATAGGGTAATCGGTCAAAGCGGTTGCGTAGTTGGATGAATCGGTTCCAATAGAACAATGCGATAGTAAGC TCTCTAGCCCCTACGCACTTATAACCCGCAGACCTGTAACGTTGGCTCACCGTGCGGCCTACTCTAGGTCAGCAGACGTTATCCGACTGAGCTAATACTT CGCAAGGGAGGTCTAGTGTATAACTGATCGACTTACAGGGTAGCAGGTGCTGAACAGCGGTAGCCAGCCGAACATAAGCCAGATTATAGAGGTCACGGAT TAGTCAGACCTTTTGTACACAAAACAGCACAAGTGACCCGGAGTATGAGTCATTGGCGGATAAGAGTCAGCGTGAGTAGTCAAGGAATCGCCAATAAACT TATTGTTCTGATACTACGGATCGGTCAGTTTGCGCACGATCGAGCCACTCTCGTCTTGCGTAGAAAGGCTGAGTAGTCGTGAAGAGACTGGGCTACTTAG AGCGGGTACTAAGATTATCAGTTTACAGAAAAAGTCTTGTAATCATGACTCCAGCTCTAAAATTGCGATTATGACATTAGCCTCTACATAACGTCTAATT TACCACTAGGCTATGGAAGGAACTTTTCAATTAGATGTACACTGCACGTGAGTCGCGTTACCGATCTGTTGATCCAATGGGCTGTTTGCTCTACGCGGTC AAGGTGGAATTCTCGGTGGACGTGAAGGCCGGGTACGTTTTTAGCCAGAGGTAGGTGCCGTTTGTAGAAGTTACCAAATCCAATGGCACTCGTTCCCCTT TTCCCGTAGTAGGCCGATTATTGACTTAGACGCCGAGTCCCGTGGTGCGACAGTCCATCAGAAAATAAATGTACTCATCCGACAGGAGAGTTATCACGAA ATTCTCCATAAAATGACCCCACCACCGTCTGATCCGCTGTTAAACGTTGGTCATCCACACTAAGTTACTGGAGTAGGGGGGCATCCGCCTAAAACGCGGG TACATCGGAGTACTAAAACACTGGAATACTCCTCTAACTAAAGAATCAGGGAAATATGTACACGGAAAATGTCAATTACTCAGATATTTCCCAGCAAGGG TACCTCTCGTACGCTTTGGGGTCGATTACGTAGGAGCTCTAATCCGTGGATTATTTCTGCCAGGTGAGGTTACCCCAACTAAAATCATCTGTGTCCCGTA TGACCCCTATGAACTCTGCAACCGGTTCCCGAACCGCCGCATGGTTGGGGGACAAATATTGACTCGCCCGCGATACGTCACGACTTTATGAAGACTTCTT ACATAATTGTATACTATCGTGCCACTCATCCTTCTAGGGCGTACTATATTTACGCTTCCGACAGTTGTGGCGGATCGAATTTTATCAGTGCGTCATAATC ATAGCCAGGTTGAATGGTTTCACCCCGACTTGTCAGAGATCTAAAATAGGGATTCAGGCAATAGTCTCGACCCCTGGTGTCCTACTTGATAGAGTACGTC TTCCTCACGAGAGGGGATCTTCTGGGCTTTCCCCAGCGCGCGTGACTGCGACGAGGACGCACATTGACGACCCTGGGTATACTGCCGTTTTAATTCAGCG TGGAAACGGCTGTGTGCATGGATTGCAGCATACACTGGCGTGGAGGATCTGATTTATGAAACATGCGGAAGAAGGAGCAAAGGTACTGAATGCTGACGAC TTGGTTATCCTAAAATCACCAGAAGGGGTCGCAACCTTCTGTATCTAAGAGAATGTTGGTATGAGTGAAGAGGCTATACTTCTCAGTCAAATTTGATTTC CTTTATCAAGACGGTGACCAGACTTAAACAGTGAGGCAGCTCATCACTGCGTGCCACTATCGAATCGGCGAGGCCGCTGACGTCGATAAGATCTGACCTC GTGTGTCCGTCGCAGTTCACCATTATCATCAACCAAAATCGAAATCACGATATGTTAGCAGGTGCATCTATTATAGTGGAAGTCTTTCAATTAGTCCCCC TGCAACCTATACGCAGTCGAATTTAAAGTCTAAGTCTATTAACTTTATAACCTTGCAAGCTGCCTTAGTAGGTTACGTGGATTCCCGTCCGAACACATCA TTAGCTCAGATGGAGACCTGGCCTAGGGGACGAATTAAGGGGATCGTGTACCGTGACGTAAGACCACCTCTGAGAGTAAGCGCGTACCATACGCAGCGCC ATGATCAATACCTGCGGTCCAGTTAAATCCTATGGCCATTGGCTAGACGTACAAGGGAGTGTCTTTCGAATTAAGCAAATTCTGCAAGCCGGTAGGGTTT AACTTCAACCGTGTCTGGTGCGGGTGAACGGGGGAGGCAATGGAACTGTGTTTCTGACTAAAGGATAGCAGGGTCCTAAAAACAATCAAAGGTTGATCAC GGATTCGACGGCAGATGATGGTCATGATGGAAGAGTTGTGGATGTGCATGCCCCTACCAATCCTTCTTATCTTCAGAGGTCTTACGTCTAGTTGTGAGGA GGACCGCATGGGATGATGCGGCTATTTACAATTAAATATAGTATCTTAACTCTCGACATCGCAAAAACACTTCAGTCAGTTCATGCAGCGTGAGCCCGAT ATCCACCACCAGCGAGCGGCCCTGGCCCGGCATGCTCGAAACTGTGATAGCAAGCCTACTCCAGGAGGATCCAAGAATGGAGATTCCCACCACTGTGTCT CTAGCACGTGTATAACTAACACTAGCATGGTACTACCTATCTTTTCGGTGCAATGTGAAATTACATACCAGCAGTCTGGGAGCTCGACAGGCCATCACTT GGATATACAGTCATCCTGAGAGCAGTCCTTGCTATTAGATCATTTTTCGACTTCCCTTATCTAGAGGCAACATATGACAGCTTCATATATAAAAGATTGA TGGACCCGCAACCATAGGCTCGTAGCTTGTGATTTTGTCTAATATAGGCTAGTCGAATTTAAGGTTTAGGTCCAGCCCGAGTTACCTTATGAAAATGTCC CAGAGATCCTTCGCACCGATCTGACTTTTGGCGCTAGTGTCAGTGCGCAAAATGGACGGCGTCGAGCGTACAAGCCGTCGCATGCCGTCAGGAAGTGGCA GGTCGACAATCTGGATTAATTGAGGGAACTAGAGCACATCTCAGGGCCAGCCGCTAAATTACTGTACGTCCCCATCCGTCAGACGGCGTGATCAGTGTTT TCAGGATGTTCCAGGATTCATAGTAACTGGATAAATTGCGTGCGCCTGGTTCCTGTTCCCCTTGATAGTTCTCCCTACGGTCCTATCCCATATCCGTCTT GTGGTGTCCGCGAGCGTCATTTGAATCGTGCAGGGCACAGGCAAACATTCACTCATGTATCCAATACTATTGATGGCCACGCTAGCCGTTTATTCGACTA GTCATCCTGACGCACAGGGGAGCTACGAAGCCTCGGGCACACATGCAAATAGAAATACCCACTTAGGCTACGTACAGAAGGAGTAAGTGAATGAAGAATC TCATGATAGTGCTTTGCTCAGGCGGACCTCTAGACCCATAAGGTGACTAGCGTGGAGTCAAACGACCAGTCGTGAGCCACTGTCCACGTGACCATCCTTG GAGTTACTATTGTAATACTGGGTATTGAGTTATTCGTCCGACCTGTAGCGGAAGAGTAGTGTTATCACCCCCGATCTCCTCCATAGTCGTTCGAAGGAGG ACTAACTGGTTTAAGGCCCCCATATGACGTTCATTTGGTTTGCGTCTGGCGAGGGGTGGATCCCCAAAGAATGGGGAACAGTAGTCATCACACTTTGAAA AGCTACACTTCTGCGTGCGCGTGAATGTTCGATGTTTCTGGAATCTTAACCTATTTCTGAGAAGGTCATGAATCCTGCAACTCCATAGCCGTCTCTACAA TCGGCCCTCTTTGTTACGACCCGTCAGCGAGAGCCCGCGAGCCTTGCTAGTAACAAACCGCCCCGGTCCGCGCCGTTTCTATCATCAGATGGCCGTATGA TCCGGCAGATCCTCCAAACCACAGTCGTATCCTATCACGCCTCCCTTAGCTGGGTCCCGTGATCTGTCGATGCGGCCACTTATTTAATCAAATCCAATTG ATGACGAGAGAAAGGCACCATTAAAGATTACAAACTGCTGAACCCTGGGGCGTAATTCCTTTCTTAACCATTTGTGCGCTGTCGAAGTTTACGAGGCCGC GGAGCCTGCTGCCGACATATGACCCCCGTGATCTTTGCTATATTCGGCTGCCGCCCGCTAACCGTATTAGTCGCCACTTTAGGCGACAAGTATTCCTGTA CAGGCTGGTTCTTTCTGCTATCATCACACCGTGGGAATCGAGAGCCGGAATCGCTGCTGCTTCCAAAGGAGTGATGTTTATTTGACAACAGGGGTCCATA GGGTGTAGTAGAATTGTCCCGTTGGGCTTTAATTCGGGCTAGAGCCTTAGTCTAGGTTGAGTACATTTGATACACAACTTATGGCTCTAACGTCATCATC TTCCGCGCGCCTTCCGTTTCGGGCACTGGGTTAATTATTGAGGTCGAGGACAAAGACGAGTGGAGGTCAACCTTATCAGTATGATCTCAATTTATATATG CACAGCGCCAAACACCGACTCAAGGTACGCCACTGGCCACTCGGCACGCGGGCTCAAACTCAAACCAAGGTGACGTGGTACCCAGACTCCTAGATTGCAA GACTATCCCCAATGCTTTTGGCGGTCTCATCTACTCGAAAATTGTCTGTGCGGGGGCGTGGCATTTCTCCCACCTGCGGCCCTAAGGAGGAAGCATAGTA CATCACTGGAAGCGACAAATGGACATGTGAATCTTTTGCCAGTATCTGAAGCGCTCCCCGCAGCCTTACCAACTTACAACTAAAGGCACCTCGAATCGCC TGGCGACGGCTCATTCGCGCTTCGATAAGGTCCTATAATCTAGGTGGTACCCGTACTCACAGTACGATATCGTTGGCCGGTAGTGAATAATCTACTATTC TAAACACGACTCTGGACTGTTTTGTAAATGGACACCGCCATACTTATTTGACTTCCTTTCACATTGTAGATGGGAGCCCGCTTGCGGACGGCTTTTATCG TTTGCACAACACAGGAAGGATCCATACTTATGGTGATTTTTTCTAACTACGCTGCATATGGAAAAGAGTCCAGGTGCTTGCAGAAGCTGCAAAAAAGGAC GCCACGTCGCCCGTAACGAAGGGCAGGGCGGGTCTTTGATATACGCTCGCCATCTTGAAATAGGCTTCCCACGGACTGAACGGCGCCGCGCTCGGCAAGC ATTTCGTGCCCGTCAAGGAAGCGCGTATCTATGGGTCAGTATTAGCATGTGACAGTTGACTTTGAGAGCCACAGCGTCTCACCCCTCGGGTCCCATAGTT ATTTATCGACTATGGCCCGTGCAAGCAGTTTCCTTAGAGAATCGTTCAATTCATCCTTGAATATCTTCGTGACTATTATCACTAGTAAACAGTGCTCGCT CCGTTCTCCACCGCCTAGCACCGACGGTATTACCTCGGTACACCCCTACTCCGAAATGCACAATTACCATACTTCCATGCTCAGGTGCCATCGTATGAAG CCTACGCGTCTCGTAACGGGACAAGCCCTGAAATTACGGGGACAACACGAGCACATTTGTGTAGCTATGCCAGATGGGAATTAGGCTCATTCGGAAATTT GTTGCGAGAATCTTTCCGTCCTGATGATTGAAGGGAATGGCAACGGGGAGATTCTTTTCTCGCCGCAGGAAAGAATGACCGGTAACTTGAGGTCCCGCAA 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 >test_sort_2 AGCGGGACTCTTCCCGATCGGCCTGGAAAAACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTAGAAAAGGTAGCCAGCGAGAAGGAAGAAGGCCAAGGCTTCATATTACCCCGAATTCTCAAGGTGGATAAGGGGAACTAACGGTTGCGAGCATGAAGCTGGCTTCAATGGCCAGGCGACAGATTTACTCCACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCGGGTTAGCACTCCCTAATTTTATCCAAGTAGGCGGACACACCCTTACCACAGGGGCTCCAATGTTCGCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTCTGCTGACGCGAAGTAACGAGTATTCTCTTGGATTTTAACTGAGCTGTCACTCCGCGTGAAGGATAACCGGCTTGAGTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGATTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTAAGGACTCCCCCCTGGCGTCGAAGCCAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGGACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTCTCGTGTGAGAGATTAATGTTAGCCCTCAGTGGGTCCCAATAAGAACCCACTAAGTAAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCGGCATAAGGAGAAACGTTATCGGCTGTCGGTGTCATCTGCCAGTGCTGGCGAGTCGAGCTTGTAGCACTCATTGAAGGTGGTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGCGCCTTCAGTGCACTGTGACAGCTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGACGCGCTCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTAGGGGGAGTGCCCTGAGGAGAGTGGAGCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACTAACTGGGGCCGTTGGGCTCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCCAGGCTGCTCAAAAGTCTGCGTAGGAGTCTCATCGGGACCTATACTAGCTGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGATTTACCTATGGACATGCGGTGTCCTAATTCATCCTACCGGAATTCTCATGCACTTCTGCCTTTCCCCCGAACATTCTAGTGGTGGGCTCGAGCTGCTGATAATCCACTCCATTGGGTGATACGAGAGGCGAGTTTGCCTTAGTTTGCGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCCGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCACGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTCTTGACGAGTGTGGGCCCAGAAGATGGAAGTTAAGGCTGGAGGAATAGATAACCATCGTACCTCCACTGGACGAGATAGCACTAAATCCTGGAAACGAGACGGATCCGAGAGTTCAGATAATGATCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAATTATAACGTCGCATACCGATTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAATCCCTAGTTCGGAAAATCGTGTCGCCCCTACAGTCCATCACTCAACTTCACTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGGACCCTTGTGTGGATCGTAAACCTACAAGTCCCTGGCACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACTATCCCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACGAATCGACCGCTCTAACTTTTGAATCTGTGGCATGGACCCCATCAAGAGGGACCAGCGTTCAGCGAGACTTCTACAAACTTACGTTACATTATGCCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACAATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGACCTCATATAGAATGTGCAATCGCTCTTTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTTCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTGTAGCCTCATGACTGCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGTCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTATTGAACCACTGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACCATTGAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGCTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGATGGTATAGGAGACATGCCTATACACGTTCGAGTGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGCGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACTCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAAATTAGGAGTCATGAGTCCGGATTGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACGCGTCAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGCTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAAAGCACCCCTATGTGTTGTCGTTTGGGCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATGTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCATTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAAAGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAGATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGTTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGTATTCGTTCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCTGAGCCACTATAAAATCAAGTGCTCTTATGATAGGGATTGCCAGGACGTTTGGAACTGGCTCAGACATTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGTATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTAAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTCTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAATGGAGCCGGACGAAGGCATGGCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGACGCACCGATATTTGGTACTTTTCTTGGATTATTAGCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT >test_sort_3 AGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCCGAATGCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATTTTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAATAACGAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATAGACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCATATACAGGGTTGTGCGCTCGTAAGGACTCCGCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAGTGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTTCAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTATGATTCGCGCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTGGGGGGAGCGCCCTGAGGAAAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCGGGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCCATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCTTACCGGAATTCTCATGCACTTCTGCCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGTGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGCCCAGGATATGGAAGTTAAGGCTGGAGGAATAGTTAACCGTCGAACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCTGATAATGGTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTGAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACCCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGCACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTTTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATCCCTCAAATCTCGTTGATGAACCCGCCCCTTCTGTTCCATTACCAATCGATGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCGTTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCCCGGTGAGCGTGCGGTCACGCATTCCAAAAGTACTCCGCGAGCCCTACGATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCGACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGGCAGGGCGTCTTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGAGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATACACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGAGCGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATCATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGATTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGTTAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACCCCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATTTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACACTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCATGCCGTGATCCGCTTACCAAATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGGTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCTAGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACCTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGCCCTTAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCTACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACCAAGGCATATCGACAGATATCTATCACAAGCCAAATACGCGGGCTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT Filtlong-0.2.1/test/test_sort.fastq000066400000000000000000000725431406722713100173530ustar00rootroot00000000000000@test_sort_1 AGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCCGAATTCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATTTTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAGTAACGAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTAAGGACTCCCCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAGTGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTTCAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGATTCGCGCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTAGGGGGAGCGCCCTGAGGAGAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCGAGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCCTACCGGAATTCTCATGCACTTCTGCCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGCCCAGGATATGGAAGTTAAGGCTGGAGGAATAGATAACCGTCGTACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCTGATAATGTTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACTCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGGACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATCCCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACCAATCGACGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCGTTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACGATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGGCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATACACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGAGTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGTTAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACCCCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAAATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCAAGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTTAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACGAAGGCATGTCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT + ,,++,+**++**,*,,,+***,+*+*+*+,*,+**,*+++*,++*,+*,++*,+,+,+,*+++*,*+**,++*++****++,**,*+,*+++,*,,,***++,*,*++,+,**+,++++++**,++,,+*,+*+,*,***+*+++,,,**+**+++**+*,,*+,++**,,,,*+,,*+**+*,+,*+*,*+*+*+++,,,,,+*+,++,+**,*+,+,+**+,,++*++***,*,+,,,,+*+,*,*,**,++,+*,++*,,,*+++,+,*+,,*+*+*,,,,,+,+++,**,+*,+++++,,,,+,++++++,,,,*,,*,+*,*++*,+,+,++,,+*,*+*+,++*,+*,+*++,,++,,,*++**,,,,+**+*,++++++*,,*+,,,,,*,*+++**++,*,*,+++**,,*,*+,*++,,,*,+*,,,+*+*++*,***+,,,,++*+,**,*,,*,+*,,**,+,,,+*,***+,++,,,***,+++,++,+,**+**+++,,*++,**,*,+*,,+**,*,*+,,,++,*,**+,**,*++,*+***,,,,+*,+,+*,++,+*+,,+*+,+++*,,,*+*++,+,,*+++*,+*,,+,+,*,++*+,*,**,+**+*+++*+++,,,+*+,,++*,*+,**,,**+,++*,+*,**+,+,,,*++*++,*,,**+,+***,+++++,,,+,,**++*,,,,*,**+,,*,,*+*,*,**+,,,**,,+,,**++*+*,,**,*,,+,,+*++**+*+***+,*+,*+,***+,*,**,,,,+,*,++,+,+,++,*+**+**+*+,+***,++,+*++,,,,+*,,*+++*+*+*,**,,,+,+,++,**+,*,+,+*,**+,+++,*,+,,,**++,*,,++***,+,,,+*++,++,***+*++*,++*++,*,,+**,,++,,*+,+***+*,++,***+,,**,+*+,,+,+**++*,**,*+,,**,,+**+*,**,+,+*+,++***+**,,+**,,++++,,**,*+*,+,+,+++,,++,+***,*,,*,**++,,**,,+*,+,*,,,,,,*,**,+,+,++*,,,+,+,*,*,***+*+++*+,+,*++****++,,,++,++,+*****+*,,+++*+**,**,,,,*,**+++*+,+,*,+,*+*,+*,,,+,++,**,*,,+,*+*+,+,+,*,++*+++++,*+**+*++,+,,********+,++*,****,,++*,*++,*+,+*+,,*+++,**++*,,++*+**++**+,+**+*+++,++**,+,+++++*+*++*,**+,+,,+,,,+*+**,*+,+**+,+*+*++,+++,,,*,*+++*,,+,+++*+,,+,,+**,*,+,+,*+,++****,*,,*,,,+*++,+,,+,+**,**++++,,++,+,,*,*,,*,,,,*,*+,+*+*,***+***+++,+*+,,,+**,+,+,*,+,,*,,*,,,,****,,+*++****++,+**+*,*,,++*,,+***+*+++,**,*+*,+*****+*,**,*,**,,+,+++*,*+,+**+*,+*++,**++*,*,+,,++,****++**,*****+++,,,++*,+*+,*+***+*,**+**++,*++,+++++,****,+*+,,,*+**+++*+,*****++,,**,++*+,,+,+****++,+*+**+*+***+,,,+,**+*+*****,**,,,*+*,*,+*,*+++***,,+*++,,,+++*,*++,+,*+,,+,++**+,***+****,,+**,+*,,+*+*+*+,,*,+*++*,**+++++,+,,,+,+***,+*,**+,*,,,,,**,,++,+,+**,,+*****+++,++*,+,+,,+*+*,,+,*,+*+,*,,,+***+**,++++++++++**++**,++,+*+++*,+*++**,*,+,+++++,*+,+++++*+**,,,,*+*,++,*,+*,,*+*+,*,,,*+**++*+**++,*+*++,+,,,,+,++,*,+*,*,,+,,*,,*,+,,**++,,*++,*,,++*,*++,*++***++*+++,,**++*,+***+**+*,**++,*,++*++*+**,*+,+*,*+*,,**,,++,,,*+**+*,*+,,,+**,**+++*+++,,,*++,+,+,**+***,,+,,*,*,*+,+,,,***+,,++,,++++,++,+*,,+***+,,*+**,*+,,,*,,+,,**++++++++*,+,++,+*+,,+,**,+*+,*,+++,*,+*,**,+**+,*+,,,+,+,+,,,,++,,*,*,+*+*,*+++*,,+,,+*+***+,++,,+*++,+,,,+++,***,,*+**+,*+,+,,,*++,,,+++,+*+++++++,*,**+*++++****,*+*,**,+***+***,,*,+*,,+*,+,+,*+,**,*,+,,,*+*,,*****++,,*+++,,+,,+,,,+,***,+++**+*+++,+,,,,,**+,+,*,++,***+++*+*,+*,+*,*+,,++,+,*,,+*+*,,+***,+,,+,+*,*****++,+,+**,+,,*,,+**,+*,+*,*,+,,,*,+***,**+**+*,,++,***+,**,,,,++++,+,+*,**,,,++,*,*+,,+*,,,+++*,,,+*++*++*,*,,,,,,,**,+,,,+,+,*+,**++,*+,*,++*,,,**+++******,,**+**,,*++*,*,,+,,*,,,+,,**++,+*+*,+,*,,,,,***++*+***,,,*+,+**,**+*,**,,+++*,+*+,*,*+,+,**+*,,,*,*+*+*,,+,*****,*+**,,*+,,++**++,,*,,*+*,**,*+,,,+,*+*,*,*+,**,,,++*,+,+,,*,,***,*+***,++,***,*,,*+++,*++*,,,,,*++,**+,,+,*,*,***+**,*,+*,,+++*,*,*,*,,**+**,,,+****,,*,,+,,*,,*,*,,,*++*,,+,,+***,*,,+,++**,+*,++,+,,,,,*++,*,++*+******++++++,+**,+,++,*+,,,,+,++*+,***,,+,,*,*,**++*,,+***,+,,*,+*+,,*+**+,,,,+++**,*,,**++**+,,++*,*+++*,+**+*,**+,,+,,,,,+*+**+*++***,++++,***+,,*,,+,,++*,+*,,,**,,,++,*++,,+*+,**+**,**++*++,++*,+,+*+*+**,,,++,++,+,**++,*,*,,,**+*+**,+*+*+***,,+,+++++*+,++++++,,,,,*,*+,**+,+*,,++,++,+**,*+*+,,*,*,**,,,,*++*,+*,*+,*++,*+,*+***,*,*****++,,,*+*,*+**,,*+**++**,,*,,++,*+,+*+,,++++,*+***+++**,*,,*+,*,+*,+,**+++**,,**++++,,*++,+*+,++++,,,,,+,*,,+,*,,,*+*,,+,+*,+,+*+++,*,,+**,******,,,*,+,+**,,,*,,,,+*,+,*++++*++,+**+*,****,,,+**+,,+++*,*,*,,,+,++*,*+**+,,++++,*****,***+*++,+**+*,*****,***,+,+*++*+**++,,**+,+*+*,+,,,*+**++*+*,*,+,+,,++,*++**++,++,,,*+**,,+,,,*,*+*,*,*,,,*,+++++,+*,*,+,*,,,+++,*,,**++,++,**,+*+*+,,++**,*+*,+*+**,*+*,*,**,,*,,+***+,***,*++**+,,*,,,,,,*,*,**,+*+*,,++,+++,+,,++,+***,*+,+,++++****+*,,,,,*+++,+,*,+*+,*+,,+,++++,*,,***,,+*,**,****,,+*+*,+,,*,**,****++*,,*+*,+*+*+,+,,,*++**,,++*,++,++++*+*,,++**+,,,,,**+,,*,,*++**+**+,+,+*+++++***+**++*,,++,+,+*,++,,,+++,*+,*,*++*+,***,++,,,****++*,*+*+++,,+,+*+,*,,*,+++,,*,+**,,,,+++,,+,+,+****+**,,+,+**,*,,*+,*,,+,*,,,+,*,+*+**+++,++*,*,+,++*+++,*+,++**+*+***,+++++**,,***+,+,*++***++*++++,,*,,+++,+***,+,++*+,,,,+*++*+,++*+*,*+,++*,,**,++*+*,+**,,++,*,*,*+*+*+++,*+,,,,,,+,++*++*+*++,,,*+*+*,,**+,**,**,*,,,***,+,,**,*+,+,,+*+*+*,,+*,+,,*,*,,,+,,,*+*++**,+,,,++*+,,,,,*+,*+,*++,***+*+,++,+++*,,**+**,+,,**++**,++*+*++,**,,,*+*+++**+*,+,,,*,,,*,****+,*****,*,++,,***+,,*,*++*,,++,,*,**+,+,,+,++*,++*,++,+*+++,,,,,,*+,,,,,+,+,+*,,*,,+,*+,*++*,*,*++,**,+*+*+++*+,*,***,,+,*+*,*,,++,,++,**,++**,*,,+++,*+,+,,**,,,****++*,+**++,*,*+*+*,,,,*,+,++,,,*****++*+*,,*+**,,*,,+*++,*++**,++,,+,*,*,++,*+*+*,,**,,,+,,,+**,+,+,,++*+*,*,,,*,,,*,***,*+++***,+,+,**,+,+*+,+,*,**,,,,,,,++,*,+,,++,++*,*,+,+*+*,*,,,******,**+,+,*++*+++,,,,*,*,++,***+,+,*,+++++,,,+++**++,***+,*****,***+*,*+*++,**,,,,,*+*,+,,*+***,**,+,*,,,,,**,,+*,**,,*+,***,**++++,*+++**+***,,+*,+*,++*++++,+*,+,**,+**,,**+,+++++*+**,+*,++,**+*+++,**,,+,*,+*,+,++,,,* @test_sort_2 AGCGGGACTCTTCCCGATCGGCCTGGAAAAACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTAGAAAAGGTAGCCAGCGAGAAGGAAGAAGGCCAAGGCTTCATATTACCCCGAATTCTCAAGGTGGATAAGGGGAACTAACGGTTGCGAGCATGAAGCTGGCTTCAATGGCCAGGCGACAGATTTACTCCACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCGGGTTAGCACTCCCTAATTTTATCCAAGTAGGCGGACACACCCTTACCACAGGGGCTCCAATGTTCGCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTCTGCTGACGCGAAGTAACGAGTATTCTCTTGGATTTTAACTGAGCTGTCACTCCGCGTGAAGGATAACCGGCTTGAGTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATATACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGATTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCGTATACAGGGTTGTGCGCTCGTAAGGACTCCCCCCTGGCGTCGAAGCCAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGGACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTCTCGTGTGAGAGATTAATGTTAGCCCTCAGTGGGTCCCAATAAGAACCCACTAAGTAAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCGGCATAAGGAGAAACGTTATCGGCTGTCGGTGTCATCTGCCAGTGCTGGCGAGTCGAGCTTGTAGCACTCATTGAAGGTGGTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGCGCCTTCAGTGCACTGTGACAGCTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTGTGACGCGCTCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTAGGGGGAGTGCCCTGAGGAGAGTGGAGCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACTAACTGGGGCCGTTGGGCTCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCCAGGCTGCTCAAAAGTCTGCGTAGGAGTCTCATCGGGACCTATACTAGCTGGCGATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGATTTACCTATGGACATGCGGTGTCCTAATTCATCCTACCGGAATTCTCATGCACTTCTGCCTTTCCCCCGAACATTCTAGTGGTGGGCTCGAGCTGCTGATAATCCACTCCATTGGGTGATACGAGAGGCGAGTTTGCCTTAGTTTGCGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCCGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGGGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCACGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTCTTGACGAGTGTGGGCCCAGAAGATGGAAGTTAAGGCTGGAGGAATAGATAACCATCGTACCTCCACTGGACGAGATAGCACTAAATCCTGGAAACGAGACGGATCCGAGAGTTCAGATAATGATCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTCAAATTATAACGTCGCATACCGATTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAATCCCTAGTTCGGAAAATCGTGTCGCCCCTACAGTCCATCACTCAACTTCACTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGGACCCTTGTGTGGATCGTAAACCTACAAGTCCCTGGCACTGCCTGTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACTATCCCTCAAATCTCGTTGATGAACCTGCCCCTTCTGTTCCATTACGAATCGACCGCTCTAACTTTTGAATCTGTGGCATGGACCCCATCAAGAGGGACCAGCGTTCAGCGAGACTTCTACAAACTTACGTTACATTATGCCGTTCGCGGTGAGCGTGCGGTCAAGCATTCCAAAAGTACTCCGCGAGCCGTACAATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGACCTCATATAGAATGTGCAATCGCTCTTTGTCGCAAAGCCACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTTCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTGTAGCCTCATGACTGCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGTCAGGGCGTCGTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTATTGAACCACTGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACCATTGAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGCTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGGGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGATGGTATAGGAGACATGCCTATACACGTTCGAGTGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGTGTGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATGATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGCGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACTCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAAATTAGGAGTCATGAGTCCGGATTGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACGCGTCAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGCTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAAAGCACCCCTATGTGTTGTCGTTTGGGCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATGTCGCCATGCAACGCACCCATTTCAGGACTTTATGTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCATTGTTCGTTAGGTTAAAGTACATTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAAAGGATAAACAGAGCCATGACATTGGCACAGCGTGCCGTGATCCGCTTACCAGATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGTTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGTATTCGTTCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGTTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCTGAGCCACTATAAAATCAAGTGCTCTTATGATAGGGATTGCCAGGACGTTTGGAACTGGCTCAGACATTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACGTAATAGAAGAACCGTATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGACCTAAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTCTATTCCAACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAATGGAGCCGGACGAAGGCATGGCGACAGATATCTATCACAAGCCAAATACGCGGACTTGCGGACGCACCGATATTTGGTACTTTTCTTGGATTATTAGCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT + 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 @test_sort_3 AGCGGGACTCTTCCCGAGGGGCCTGGAAATACCACGGATTCCAATAGTACGTTCTGTTAGCTGGGTGTCTTTATGCATGCCAAGGTTCGAACCTCCGGCCATGTTGGATGTTGAAAAGGTAGCCAGCGAGATGGAAGAAGGCCAAGGCTTCATATTACCCCGAATGCTCAAGGTGGATAAGGGGAACTAACGGCTGCGAGCATGCAGCTGGCATCAATGGCCAGGCGACAGATTTACTCTACTTTCCGCTGCTTGTTCTGGATTGGGATGTTAACCCTTTGATCCCACAGAGGTCAGCGGAGAAAACAACGTCTAAATATAGTCGGACAGACTGTAGCGACCCGGTTAGCACTCCCTAATTTTATCTAACTAGGCGGACACACTCTTACCACAGGGGCTCCAAGGTTCTCTCATTTCCGTGGGTGTAGCAACGTCCTATTCTTATGCGGACGCGAAATAACGAGTATTCTCTTGGATTTTAACTGAGCTATCACTCCGCGTGAAGGATAACCGGCTTGACTAAAGGAGACGGTCAGTCGCAGAGGGCAACGCGCTATAGACATTCGGTTTATTGAGTTCACCCTGGATAGTGACGCGGCCCTGGTTGTAGGTCGGGGCAAACTCTGAAATTGCTACTCATATACAGGGTTGTGCGCTCGTAAGGACTCCGCCCTGGCGTCGAAGCTAGTTCGACAAAATGGCCCCGGAAGACGTTATCTAGATGAGTCTGGGTGGCTTTCAAGAATTCGCCTATCTTGGTACCTGGCCCTAACGATCAGACAGGCGCACTGCTGATGAGGGCATGTACCGACCATACGGTTTTTCTTCAGGCAAATGCCCGTTCCGGTGTCCGTGGTCAAGACTCAAAATCTCCCCTAAGGCTGTCACGAATTAGAGTTTCGTGTGAGAGATTAGTGTTAGCCCTCAGTGGGTCCTAATAAGAACCCACTAAGTTAGCCTCGCCGGTACGCAGACCTAGAGACTTGTTGGTGGGCAGCTTCTGCGCTGTTGGCATTCCTGCATAAAGAGAAACGTTATCGGCTGCCGGTGTCATCTGCCAGTGCTGCCGAGTCGAGCTTGTAGCACTCATTGAAGGTGCTGTGAGCTGCACGCCTCCACTAATTTAATCCCCGTCGCAAAGTTGGTCAGACCAGGAGCCTTCAGTGCACTGTGACAACTGGAATTCCGACTCCGGAAAAGTGCAATGGGTGTCCAGTGGACGACTTCTTATGATTCGCGCCCGCGCCGCGTCCACAGGGGGACTCGGCGAAAAGTACAAAAGTGGGGGGAGCGCCCTGAGGAAAGTGGATCGTGCCGTCACCGGGTGACGGGTACAAGAACCTACTACTAGTGACGATGCTCTTAGATGGAGTGTAGCACGATCTTCACGGATAAAATAATGAAACAAACTGGGGCCGTTGGGCCCCCGAAGTATATGAACTGCGGCCATGTATGTTATGAATCCCCTCGGGTTCGGGGCTGCTCAAAAGTCTGCGTAGGACTCTCATCGGGACCTATACTAGATGGCCATGCGGAATACAGCGGCCTAGCTTTTATGCGATGTGCGGGGATAATTCTAATAGTTGCGGCTGGTCCATAACCGTGGACGTCGTATATTGGCAGACCTGCACAGTTGGTCCGATTAGACATACAGCACCCTTCTACATCCTGTTGTTCTGGAGAAGCCCACTCTAGTAGGACGGTTTACCTATGGACATGCGGTGTCCTAAATCATCTTACCGGAATTCTCATGCACTTCTGCCGTTCCCCCGAACATTCTAGTGGTGGGCTCGGGCTGCTGATAATCCACTCCATTGGGTGATACAAGAGGCGAGTTTGCCTTAGTTTGGGCAGGTCATGAAACCAAGCAACCGCATGCTTAGCTGGCTAATTGTTCGACTCGGCCACCAGCAAACTGTCGAAACGCGTAGAAAGTACAATTCCGTAAGCGAGTTCGGAATCCAGACCCGCGGCAATGCCCTCTCTTCCTACGCAGCGACCCCCTGTTTACACAGACAAGGCCCCGATGGCCAATCGTGAGTCTCGACGAGTGCCATAGAGGCGCCCTACCGGCGTCAGTGGGGGTGATTAATCACTTCACTGGTTCGCGGTGGGCTACGCGCTCACCTAGCTTCCCCGTAGTGTATTGACGAGTGTGGGCCCAGGATATGGAAGTTAAGGCTGGAGGAATAGTTAACCGTCGAACCTCCCCTGGACGAGATAGCACTAAATCCTGGAAATGAGACGGATCCGAGAGTTCTGATAATGGTCGAGATGGGACAACAGGCGAATCTCGCAAACGTGCTTGAAAATATAACGTAGCATCCCGCTTATGGGCTTTATCGCGAAACCTGACAGCGCTCCCTCAAACCCCTAGTTCGGAAAATCGTGTCGCTCCTACAGTCCATCACCCAACTTCAGTTGAGCAATAAGTTTAGGGCGCGTCACTCTATCTACTTTACTGCTGAATGCACCCTTGTGTGGATCGTAAACCTTCAAGTCCCTGGGACTGCCTTTTCTTGCGATGATAAGATTAAGTGTTTGCCCGTTTGCCAACCATCCCTCAAATCTCGTTGATGAACCCGCCCCTTCTGTTCCATTACCAATCGATGGCTCTAACTTTTCAATCTGTGGCATGGACCCCATAAAGAGGGACCAGCGTTCAGGGAGACTTCTACAAACTTACGTTACATTATACCGTTCCCGGTGAGCGTGCGGTCACGCATTCCAAAAGTACTCCGCGAGCCCTACGATCGACCTCTAGTATACTCCTGGTGCGGAGCAAACGATTTGAGCAGGCCTCATATAGAATGTGCAATCGCTCATTGTCGCAAAGCGACAACCAGTCACGCCGTTCGTCGACTACCACCCACTTCGTGGATGTGCGCCAAGTCTGTCTCCAGCTCTCAAGACTCATTGCAACTTGCCAATAACCTCAGTGCCTTGTCACTTCGAGTTCTTGAGGAATAGTCAGGGTCCTTAAACTTCATCGACTCGGCACAGTCTCTAGCCTCATTACTTCCCATTCCGGCCCGATGTGGAAGCGGTCCGGCTACTCAAATGCTCCCGGAGACCGAAGATTTCGGGACCTTTCGTTGTCAGACGCCAGTCATTGTTGCCTCTAACTGCATTGGAATCTGGCCCAAATAGAGATTGACCTCGGGAATCCGTCGCTGGGCAGGGCGTCTTACTCTGAACGGGACATCACTCCCGACCACCGGGGGGCGTCGTAGGTGCAGCAGACTGACAAATCCGTCCCAGTCGACTTATGTTCGCATGTCGTTATTAACTGCCTTGTACCTACGGTATGTACTGAACCACCGAGATCAGGGCAGGTTGAGCAAATTCATATCTCAAGGTGTGTCGAGAAAAAACGATTTAAGCTCTAGACCGGGAGTGCTGGTCAGAGTGTTCTGCTCACAAGGAAGCAATTCGGTGTTGGGTACCAATCAGAAATCACACCGTGGCTCTGAGCTTAATCCCTTGAGTTGGGCAACCGGGAGGACCAAGAGTACCGGCGACGGCCTCTGATTCTTGACGGTATAGGAGACATGCCTATACACGTTCGAGAGTAGTGACAGCATAGGATGCCTCAACGCTTTTACTCACGAGTGAGCGGTCCAATCTTGATGTCTAGACATGAGTTTTTCTAACGAGTGAACGGTATCCTATCCGCCCTCTGGATCAGCGGTTTCGGGGGTCCCTATCATGCTGTTCATATGCTCTGTCCATTGATTTGAGCCTACGGTATGAAGCACTCGCGGTGAAAAGGTTGCTGTAACCCGCGGGTGGTTACGATCTAATCTTCTGGTACGAAGGCGTGCCGTTACGCCAAAACTGGTACCTTGGTGTTTTTGCTGATCTCCAGAATTTTTGCTGTACTATTCCAATTTAGGAGTCATGATTCCGGAATGTCATTCCGACAAGCAGTATTCCCAACAGAGAACACTTGCATACTCGTTAATGATGAACACCTCTAATACGGAGACGCACCAGGTCTCCTAATCCCGGTTGTTTGCGACTCTGTTGTATATGATCAGAGAAAGGCGTATAAGAGCACCCCTATCTGTTGTCGTTTGGTCCGGACTCTTTAACTTCGGACCCAGACTCCCCCATTTCGCCATGCAACGCACCCATTTCAGGACTTTATCTGAAGCCTCTGCAAGTAGTACAGATCGCTTGCCCTCCTTGTTCGTTAGGTTAAAGTACACTTGACGATCACAGCCCCCATGTTCTTCTTAAGTCCTGGGCCTGCAAGACATGGTAACACTATAACCAATGCTACGTAATAAACGGATAAACAGAGCCATGACATTGGCACAGCATGCCGTGATCCGCTTACCAAATGATAAACTTGGTGATAATAGCAGTTTATTTGTAAGCCGTCGCGTGACAGAGGTCTGTAACTCTGATGAATTCCGAGCGCGCGTCGTCTGAAGTTGGTTGGCTAGGATTCGTCCTTAATGCAGTCTAAGCTGCCAGTAGCCACGAGAGGTAATTCACAAAGGTGGTACGCTGCTTCACAGTTCCAGGATGTAGAGTCAGAACCACTATAAAATCTAGTGCTCTTATGATAGGGATTGCCAGGTCGTTTGGAACTCGCTCAGACAGTCGCCGTCATAGAAATACATTACCTAAGCCCACGTGATATTGAACAGACCCCGCTATGGGATATAGTATACGAACCTAATAGAAGAACCGGATAACCTTGGTAAATATCTTCCAGAACGGGTCCGGCCGTTTAGCGTGATTTCAGCCCTTAGTGTCAACTTCTCTACTTGCTGGTGGATAAGTATTACCACTTTTATTCCTACCGGTAGACTACGCCATGAGTGTGCGAGGACGCAGTGGAGCCGGACCAAGGCATATCGACAGATATCTATCACAAGCCAAATACGCGGGCTTGCGGAGGCACCGATATTTGGTACTTTTCTTGGATTATAATCGTTTGGTAGGCCTTGTGGTAACCATGTGAGCGTCGTGGCGCTCGGAACTACTTT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 Filtlong-0.2.1/test/test_sort.py000066400000000000000000000440371406722713100166620ustar00rootroot00000000000000""" Copyright 2017 Ryan Wick (rrwick@gmail.com) https://github.com/rrwick/Filtlong This module contains some tests for Filtlong. To run them, execute `python3 -m unittest` from the root Filtlong directory. This file is part of Filtlong. Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with Filtlong. If not, see . """ import unittest import os import subprocess def load_fastq(filename): reads = [] with open(filename, 'rb') as fastq: for line in fastq: stripped_line = line.strip() if len(stripped_line) == 0: continue if not stripped_line.startswith(b'@'): continue name = stripped_line[1:].split()[0] sequence = next(fastq).strip() _ = next(fastq) qualities = next(fastq).strip() reads.append((name, sequence, qualities)) return reads def load_fasta(filename): reads = [] with open(filename, 'rb') as fastq: for line in fastq: stripped_line = line.strip() if len(stripped_line) == 0: continue if not stripped_line.startswith(b'>'): continue name = stripped_line[1:].split()[0] sequence = next(fastq).strip() reads.append((name, sequence)) return reads class TestSort(unittest.TestCase): def run_command(self, command): binary_path = os.path.join(os.path.dirname(os.path.dirname(__file__)), 'bin', 'filtlong') input_path = os.path.join(os.path.dirname(__file__), 'test_sort.fastq') input_fasta = os.path.join(os.path.dirname(__file__), 'test_sort.fasta') assembly_reference = os.path.join(os.path.dirname(__file__), 'test_reference.fasta') illumina_reference_1 = os.path.join(os.path.dirname(__file__), 'test_reference_1.fastq.gz') illumina_reference_2 = os.path.join(os.path.dirname(__file__), 'test_reference_2.fastq.gz') command = command.replace('filtlong', binary_path) command = command.replace('INPUT', input_path) command = command.replace('FASTA', input_fasta) command = command.replace('ASSEMBLY', assembly_reference) command = command.replace('ILLUMINA_1', illumina_reference_1) command = command.replace('ILLUMINA_2', illumina_reference_2) output_name = 'TEMP_' + str(os.getpid()) command = command.replace('OUTPUT', output_name) try: self.output_file = [x for x in command.split() if output_name in x][0] except IndexError: self.output_file = '' p = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) _, err = p.communicate() return err.decode() def tearDown(self): if os.path.isfile(self.output_file): os.remove(self.output_file) def test_sort_high_threshold_1(self): """ With a high input threshold, all three reads should be outputted. """ console_out = self.run_command('filtlong --target_bases 100000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 100,000 bp' in console_out) self.assertTrue('not enough reads to reach target' in console_out) def test_sort_high_threshold_1_assembly_ref(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 100000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 100,000 bp' in console_out) self.assertTrue('not enough reads to reach target' in console_out) def test_sort_high_threshold_1_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 100000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 100,000 bp' in console_out) self.assertTrue('not enough reads to reach target' in console_out) def test_sort_high_threshold_1_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 100000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 100,000 bp' in console_out) self.assertTrue('not enough reads to reach target' in console_out) def test_sort_high_threshold_1_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 100000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 100,000 bp' in console_out) self.assertTrue('not enough reads to reach target' in console_out) def test_sort_high_threshold_2(self): """ With an input threshold of just over 10000, all three reads should be outputted. """ console_out = self.run_command('filtlong --target_bases 10001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,001 bp' in console_out) self.assertTrue('keeping 15,000 bp' in console_out) def test_sort_high_threshold_2_assembly_ref(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 10001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,001 bp' in console_out) self.assertTrue('keeping 15,000 bp' in console_out) def test_sort_high_threshold_2_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 10001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,001 bp' in console_out) self.assertTrue('keeping 15,000 bp' in console_out) def test_sort_high_threshold_2_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 10001 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,001 bp' in console_out) self.assertTrue('keeping 15,000 bp' in console_out) def test_sort_high_threshold_2_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 10001 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,001 bp' in console_out) self.assertTrue('keeping 15,000 bp' in console_out) def test_sort_medium_threshold_1(self): """ With an input threshold of exactly 10000, only two reads should be outputted. Read 1 is the worst (by Phred score), so just reads 2 and 3. """ console_out = self.run_command('filtlong --target_bases 10000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_2', 'test_sort_3']) self.assertTrue('target: 10,000 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_1_assembly_ref(self): """ With a reference, reads 1 and 3 are the best two (instead of reads 2 and 3). """ console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 10000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 10,000 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_1_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 10000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 10,000 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_1_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 10000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 10,000 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_1_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 10000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 10,000 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_2(self): """ With an input threshold of just over 5000, two reads should be outputted. """ console_out = self.run_command('filtlong --target_bases 5001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_2', 'test_sort_3']) self.assertTrue('target: 5,001 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_2_assembly_ref(self): """ With a reference, reads 1 and 3 are the best two (instead of reads 2 and 3). """ console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 5001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 5,001 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_2_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 5001 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 5,001 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_2_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 5001 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 5,001 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_medium_threshold_2_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 5001 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1', 'test_sort_3']) self.assertTrue('target: 5,001 bp' in console_out) self.assertTrue('keeping 10,000 bp' in console_out) def test_sort_low_threshold_1(self): """ With an input threshold of exactly 5000, only one read should be outputted: read 2 (best by Phred score). """ console_out = self.run_command('filtlong --target_bases 5000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_2']) self.assertTrue('target: 5,000 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_1_assembly_ref(self): """ With a reference (instead of Phred), read 1 is the best. """ console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 5000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 5,000 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_1_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 5000 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 5,000 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_1_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 5000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 5,000 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_1_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 5000 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 5,000 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_2(self): """ With an input threshold of 1, only one read should be outputted: read 2 (best by Phred score). """ console_out = self.run_command('filtlong --target_bases 1 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_2']) self.assertTrue('target: 1 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_2_assembly_ref(self): """ With a reference (instead of Phred), read 1 is the best. """ console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 1 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 1 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_2_read_ref(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 1 INPUT > OUTPUT.fastq') output_reads = load_fastq(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 1 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_2_assembly_ref_fasta(self): console_out = self.run_command('filtlong -a ASSEMBLY --target_bases 1 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 1 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) def test_sort_low_threshold_2_read_ref_fasta(self): console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --target_bases 1 FASTA > OUTPUT.fastq') output_reads = load_fasta(self.output_file) read_names = [x[0].decode() for x in output_reads] self.assertEqual(read_names, ['test_sort_1']) self.assertTrue('target: 1 bp' in console_out) self.assertTrue('keeping 5,000 bp' in console_out) Filtlong-0.2.1/test/test_sort.txt000066400000000000000000000005571406722713100170500ustar00rootroot00000000000000These reads all come from the same sequence in the reference. test_sort_1: Phred scores around 10 (worst), exact match for reference (best) test_sort_2: Phred scores around 20 (best), error rate of about 3% (worst) test_sort_3: Phred scores around 15 (medium), error rate of about 1% (medium) Quality order by Phred: 2, 3, 1 Quality order by reference: 1, 3, 2 Filtlong-0.2.1/test/test_split.fastq000066400000000000000000000553501406722713100175140ustar00rootroot00000000000000@test_split_1 TCATTACGTTTGTATTTTTCCGGGTTGACAAGAACGCCCATCTCACCCCGGCTTGTATTGCGCCGTCAGGAAGGTTGCGAAAACCTCAATGCAGGCCTCAGCGCGGCGAAAGGCGCGACCGTGTTCACAATCACATAGCTATATGCGCTAATAAGATTGGGGCGGCTCTCATTTCGAGAGCCGGATCTTTTGTTTGACAGACGTTGTAGTTGGTAGTAGCTACGATGTAGGCAACCACAGGGGCTCCGAAGTGAGTAGGGACTACTTAAAACATTTCCGGGGAGTAGAAGTAACGATTTGGAATACCGGGGAGGGTGGGCATCCGTCCCTTGGTGAAAATCTCGCAAGGGTTACACGACATTGAAATTCACCAAAAAATTCGCGGCGTATAGTAATCTTGCCCTACTTTGCTATGCCCTACTAGTGGGGGACTCAAATCGACTGGGTGCAGTCGGGAATAAGTTCAGCCCCGAGCCCGGGCCCACTCGTAGAGGCGCGCTCCCTTGGGTCTAGAGACATGAAGCGCACTCACGGTCCACAGTTTTGGTGGAAACGCTGTCAATACTACGTTCACTTGCCCATACGGTTATATAGGCGTTCGGAGCGCGATTGCAGGTGGGGATATAACCACAACGCGGGGGTGAGGACTGAACATCCATACCGGCTTGTACCGCGTGGGATCAAGACACTTGCTATCTGATCTAGCTTTTCGAAGTATTGCTCGGTGAAAGTAAATTGTGCCAGGTAGACTTATCACGTCCTGATACGGGAACATGGACTTGTTACTGCGGCGTTGAAATCAATCCTTGCTCTTCTGCCATGTGTATTGTGTTTCCACTCTGTATTTTGCCAGCGTCGTACATGGCACAGCTTTGACCCGTTGCAATGAGTAGGATATACAGTGTACTCGCGATTAAATGGTGTCACATGCCTTGGCGCCATGCGGTATAGTTCAGGCGCTCGTATTCCGGACCCGCTTCAAAATGGCGGCAATTGGTTGTCGCTCCCCGCCCCAATGGTATTGTGCCTTGTCACGATTCACGTTCCGTCTGATGAATGAATACTTCCACGTAGCACGCCTGCATTGCAGCTCTTTCCGCGAAACGTGACTATCGCGGGCCGGGTATCCTATTAAAGGAAGCACCACCTTTAGCCTACGCTATAGCCAGATCAGCCACAAGACGTTTGACAAAGCGAATACCATGACATAAAGTCCGACAGGGCGGCAATGAGCTCAATATATTATTATTATGGACAGATTTTTAAGGTACGGCTTCAAGTCGATCGCACAACAGTGCTGCTTCGGTTCCCTGGAGCAGGCGTACAAGTACAAGCCTTTTAAGGAGACCAAGTTTATGAATATCTTGAGGCCCCCGCTCAGCAGTACCATTTATACTAAACGGAAGTCGCCTGGACAGTTAATTCGTACCTATATAGCCTGCTGGTAAGCGGCAAACCGCTGTGTCTGTACCATATTCAGGGAGTTCACGTGTCGATACCTCGAGTGCGGGAGCGCACCCGGGCGCCCTAATCGTGGTCGCCCTAGGTCTCCTCTACTATTGGGAAGCCATAACGCATCCTCCAGGGTTTGCCGGTAGCTTCCTTTGGTCGGTTAGCACTTCACTAATTTCGCGGTACTATTCAGTTGACGTCTGGCTCTCAAACGTTCTGGACTGTTACTCTTGCGGAGTTTATGCCCCCGATCTTAATTCGAAAATAGGCTTCAAAACGCTCCTTGTCAGGCCAGATATTCGTGCTGTCGGTGGCGCTGCAAGCGCAATACTGAACGCTCGCAGGTCCGATGGGCCCCCTGGTCATCTGTTCATCTTAGCAATAGAGGTCTTAATAGCAAGGACGTTACTATGTATCCTGCCCAGCTGAAGAAGTTCATCTCTGCTCACGTGTGACGCTAACTCGATCCATGTTAGCTCACTAGCGTTAGGCTGGGGGTCTCTGACCACCATGACTTCTCATGCGGGAGGGGCCGGCCCGATATTCATTGGGTCGTCGAAGCCCCTGCTTCTGTGACGACCTCGGTGGCCCAAAACGCACTGGGATGCAATCGGCTTTCCGTGGTCTCCCAGTAAAAACCGCCAGTTATTCTAGTTGCTGCTCGTTCCCTAGGTGGTTAAAGGTCCCCAACAACGAAATGGGTGAGTCTTACATTACGGACCTGGAAATCTACAAAAGCCTACGTACAAGTACGCACTTGGTCGTCAATGCCCTAGGCGGATGGGGACCCCCCGCCCTAGGTCCCAGATCCAGCTTCTTTGACGTCACATGTGCCATTATGGACTCTGTTCCGATAGGTCAATAGGAAGAATCACTCACTCTGATAAAAATTGACACCGCTGGGTCCTAGCTCGGCAAATGCAATCCCCCGGATAAGTCAACTCACGGGGCTTGCAACATCGCTTTGGAGAACGACTGATAGGCAACCGATCTGACGGACACGGCGCTGGAAAACTCAGGCATTACTCACCGTGCGAAATTCCTTTCCTAATTTCTTCTAACGGGACCTCCACGATTGCTGTTTTTGTATATGTGTTGCGAATGTTAAAACAGACAGGGATCAGACCTCTAGAATAATGGAACATTTAGGCCAAAGATCGCAGCTGCATTTTTTCCTCTTAGCTCTCGCACCTCGTTTAAGCATCGCGGAGCCTGAACGTATTCACTTGTATCCTCACCATTCTGCGTCATTATCGAGTGACCCCTGTCGAGCCAACGCGAGCTCTCAGTCTCGCGAATTCCGACGTTAACCAATAGTCTTAAGATTTTAATATATTCCGGAAGGTGTGTTACCCATTGATCACCAACGGTATGGGTGAAAGCTGTTGTAAAAGTCAGACTAGTTTAGAGCGAACCACATGGGGTCACATCTCATTAGAGAAAAGGAC 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@test_split_2 TCATTACGTTTGTATTTTTCCGGGTTGACAAGAACGCCCATCTCACCCCGGCTTGTATTGCGCCGTCAGGAAGGTTGCGAAAACCTCAATGCAGGCCTCAGCGCGGCGAAAGGCGCGACCGTGTTCACAATCACATAGCTATATGCGCTAATAAGATTGGGGCGGCTCTCATTTCGAGAGCCGGATCTTTTGTTTGACAGACGTTGTAGTTGGTAGTAGCTACGATGTAGGCAACCACAGGGGCTCCGAAGTGAGTAGGGACTACTTAAAACATTTCCGGGGAGTAGAAGTAACGATTTGGAATACCGGGGAGGGTGGGCATCCGTCCCTTGGTGAAAATCTCGCAAGGGTTACACGACATTGAAATTCACCAAAAAATTCGCGGCGTATAGTAATCTTGCCCTACTTTGCTATGCCCTACTAGTGGGGGACTCAAATCGACTGGGTGCAGTCGGGAATAAGTTCAGCCCCGAGCCCGGGCCCACTCGTAGAGGCGCGCTCCCTTGGGTCTAGAGACATGAAGCGCACTCACGGTCCACAGTTTTGGTGGAAACGCTGTCAATACTACGTTCACTTGCCCATACGGTTATATAGGCGTTCGGAGCGCGATTGCAGGTGGGGATATAACCACAACGCGGGGGTGAGGACTGAACATCCATACCGGCTTGTACCGCGTGGGATCAAGACACTTGCTATCTGATCTAGCTTTTCGAAGTATTGCTCGGTGAAAGTAAATTGTGCCAGGTAGACTTATCACGTCCTGATACGGGAACATGGACTTGTTACTGCGGCGTTGAAATCAATCCTTGCTCTTCTGCCATGTGTATTGTGTTTCCACTCTGTATTTTGCCAGCGTCGTACATGGCACAGCTTTGACCCGTTGCAATGAGTAGGATATACAGTGTACTCGCGATTAAATGGTGTCACATGCCTTGGCGCCATGCGGTATAGTTCAGGCGCTCGTATTCCGGACCCGCTTCAAAATGGCGGCAATTGGTTGACCGAGACACTTAGTGCTATGGGGTCGCATAATTGTCATATTCAGATATGTGATGAATGAATACTTCCACGTAGCACGCCTGCATTGCAGCTCTTTCCGCGAAACGTGACTATCGCGGGCCGGGTATCCTATTAAAGGAAGCACCACCTTTAGCCTACGCTATAGCCAGATCAGCCACAAGACGTTTGACAAAGCGAATACCATGACATAAAGTCCGACAGGGCGGCAATGAGCTCAATATATTATTATTATGGACAGATTTTTAAGGTACGGCTTCAAGTCGATCGCACAACAGTGCTGCTTCGGTTCCCTGGAGCAGGCGTACAAGTACAAGCCTTTTAAGGAGACCAAGTTTATGAATATCTTGAGGCCCCCGCTCAGCAGTACCATTTATACTAAACGGAAGTCGCCTGGACAGTTAATTCGTACCTATATAGCCTGCTGGTAAGCGGCAAACCGCTGTGTCTGTACCATATTCAGGGAGTTCACGTGTCGATACCTCGAGTGCGGGAGCGCACCCGGGCGCCCTAATCGTGGTCGCCCTAGGTCTCCTCTACTATTGGGAAGCCATAACGCATCCTCCAGGGTTTGCCGGTAGCTTCCTTTGGTCGGTTAGCACTTCACTAATTTCGCGGTACTATTCAGTTGACGTCTGGCTCTCAAACGTTCTGGACTGTTACTCTTGCGGAGTTTATGCCCCCGATCTTAATTCGAAAATAGGCTTCAAAACGCTCCTTGTCAGGCCAGATATTCGTGCTGTCGGTGGCGCTGCAAGCGCAATACTGAACGCTCGCAGGTCCGATGGGCCCCCTGGTCATCTGTTCATCTTAGCAATAGAGGTCTTAATAGCAAGGACGTTACTATGTATCCTGCCCAGCTGAAGAAGTTCATCTCTGCTCACGTGTGACGCTAACTCGATCCATGTTAGCTCACTAGCGTTAGGCTGGGGGTCTCTGACCACCATGACTTCTCATGCGGGAGGGGCCGGCCCGATATTCATTGGGTCGTCGAAGCCCCTGCTTCTGTGACGACCTCGGTGGCCCAAAACGCACTGGGATGCAATCGGCTTTCCGTGGTCTCCCAGTAAAAACCGCCAGTTATTCTAGTTGCTGCTCGTTCCCTAGGTGGTTAAAGGTCCCCAACAACGAAATGGGTGAGTCTTACATTACGGACCTGGAAATCTACAAAAGCCTACGTACAAGTACGCACTTGGTCGTCAATGCCCTAGGCGGATGGGGACCCCCCGCCCTAGGTCCCAGATCCAGCTTCTTTGACGTCACATGTGCCATTATGGACTCTGTTCCGATAGGTCAATAGGAAGAATCACTCACTCTGATAAAAATTGACACCGCTGGGTCCTAGCTCGGCAAATGCAATCCCCCGGATAAGTCAACTCACGGGGCTTGCAACATCGCTTTGGAGAACGACTGATAGGCAACCGATCTGACGGACACGGCGCTGGAAAACTCAGGCATTACTCACCGTGCGAAATTCCTTTCCTAATTTCTTCTAACGGGACCTCCACGATTGCTGTTTTTGTATATGTGTTGCGAATGTTAAAACAGACAGGGATCAGACCTCTAGAATAATGGAACATTTAGGCCAAAGATCGCAGCTGCATTTTTTCCTCTTAGCTCTCGCACCTCGTTTAAGCATCGCGGAGCCTGAACGTATTCACTTGTATCCTCACCATTCTGCGTCATTATCGAGTGACCCCTGTCGAGCCAACGCGAGCTCTCAGTCTCGCGAATTCCGACGTTAACCAATAGTCTTAAGATTTTAATATATTCCGGAAGGTGTGTTACCCATTGATCACCAACGGTATGGGTGAAAGCTGTTGTAAAAGTCAGACTAGTTTAGAGCGAACCACATGGGGTCACATCTCATTAGAGAAAAGGAC + /04//11,-/-)(&&+***)/1,'0(%&$#$&&&1*+0,))'(%(%&%'4433324/.,''(++&12022..0./0&(331110233//1%$'((224.(1++)30/.+,0.1**/10-,-2.,%)****1.-.)0001-,-)+..,-,4,)($*'+0.23+&+)*310.+(%''(,3442////03(,')0,+13./-,*('($$&'',,/+*))(%'$+--.1-/032.2-,,+*%&&'&&(*,'&#('**')((-,)+()'(($'%$##%&),*)&&$"'$%,'$#'$(0,"#$)''*0/452550+,1--02)).+',,,245.-0..(,,.0,*.,+,)&%'(&&-*,&+2)%&,+,1-4.-0/.../*)2420&(&-.++$+)',,(-1+(01/2-+-)(#)+43/+-))('''('$')),-++*-4'%*),)*0--)++-,++(*'+')4200+-++&+$&')#'-03+/01443,*'),10.,+*-+%$%%$##$$&&+$+.-+''*$(+'(-0,/.+*',42131(',('(&)((,,-....1.*,)-3/1,,&##%%().34214-'++''+/0.+*+$+-240*0++..31-,/+-0--)--.,.(&+%%#('*))%)#'('&(*+***),,,....20/.-2,0.)'#%&(*./('$%$%&&'*+())*(#,(''+)(**)'($&(*'$)+,,+.++41*-2,2432213-31-.1.+&%&%&-+./.,,'%&&(-/10***(+1,+-,2)++*.+*,.,)*,,12/-+*&''*)+-+143/(('-,,,),012322,3,+,-0,-11,+3.,13,'#$*#-,++%042+,+&')..$),&#%#%$,)01--3*-(%&&$(%*0&*120.+0/301./'%%%&()+*,01&,01220121,-$&-0332**'++2+230.*$0*&')+)+&/.,'-&,1/+*(**.)+%)%)%.4144235/2222231454542/&24131,403144441*/'00/412111210+)'(+.31/3.0524041.4-1)/43/**)($,))),)-1-/04505-32533155443+,+++11+3+%'#$-./3,+,&%&*,-.--3.,0.%,--000.---,00522/-*)*'+$$$)$$,1123122*),)(2*.22*&$%')(.*$%&')+*'+%**1+)&)$&&*(&-3304*'$'%$)$%%&('')/**0+41012.,.4,)+-2/./.0445.+)+)*%(**'/,*.+((3-+*-('(.+('%&%&*)(*-(%+.112534.-./).3..34-/3201/,,5/,(*'((+)*2232(*'/.*.12)1,+)*+%""#%'&)*-2.)2)%&()).-(*%''%'#))*,*(()210)(''-.$#*+)-.4554231-*)&'*(431351130)$&',1)&%+)+)0%+033++.--...-,+/142-0+--0./0,)(/(),)++)+*&%$%%,)(,000-0/*)%-,013/(5,*0(0.)+)(.)3231*,+%'(%%%#)(),.110'-&*-+)'/&('(+.031101+.-.,,(.--53&2//.-)*+*+1-/+,-3,1.&('(.*+3()'#)#'*0//-('*))-)%('00/'+...321/,00-**'(./1-/01210111111+-1/1*-3/-'''$'#)'+,))(1+...042443120/04.--%(,-+)/.03(2+211.%(++-30+-*$'%$*+++--,'*++00-*('('#$'*.(,.%*/$00/.*(*),)+,,/,(&())''()+,*+%-/+,.-55/-.(*33.-+++22/1.*,*((*,./-(*)%.013//4/31*'.+)&('%$(-0.-,)+)++&&+03,0+2323445..3,)0,,24.*(/2-.-&,402534212*11/&'%%'/-+*)&13/4221455432.43,110355443333./#$'%)(('&),2,()(*)..450144.....3/.-*&./0./-&.()'$%)**--.,1//%+(%'))++,13./3.3201-()(,((&,,)%%,-.)3/25/*)1/)%%)++4334*+0-*%#&'''$$)+*+%+(++1%(%**4/21-12/'22,+1.-3+*2&1+,,.22030-*,+/0.32.2./''+%()*1*'&)%)*.2403321.()$&#$$*,-+',4**35321&('#%+*,,114345333322.*+54,((&)+401+&#$*,--4,+/,)'*&&-/42234-(-&,1.'.0./4224+&%(#$#$%&)(')0&+4323502)%%&(..3322-)%-0).-)('%*((.0/,/)/+'&*&&%',0++++,32324220.1'+,,/0(40-)(02.-,-./3//3.-/1**#%$'(//-+.+.3).1.((+)).10..&2.//12*)0$$,**++(&*,((2/345/50.3/-)&%'&%%()1,)(1-)/,(,'')*1((%11-221*4440533553232-5*//-,+)-,,$0,-+(0&+.3243///.*(%$*+04.)&%($'*2-+'+))+,'(&+-,-'&.%'+#''+)&(('%()%&*%',(--2)'%$&,03//+((&$%$)(/0442-)3/1211&))(%$+,./5-'+$--,+$$(302231/0.,+&&..-/.2.03/01,(,)%+&(0,+)*+.,&,',)$&,.12,,)*,+*%()1.20,.,0/*/32153,)3,,3)12/13/'',//0/,-.3244/++,'0*(.'()&..1233320/0134)$'&)2,,21/124('*()1+*(33-)453432343,*&%((.233./0*./-*((*..,,$*+))&4541111031&&&%&,*242/**%(+1-11/.2$15--2544540+)'&(#&'*1-.2/.10...)$&#**&('(),/+ @test_split_3 TCATTACGTTTGTATTTTTCCGGGTTGACAAGAACGCCCATCTCACCCCGGCTTGTATTGCGCCGTCAGGAAGGTTGCGAAAACCTCAATGCAGGCCTCAGCGCGGCGAAAGGCGCGACCGTGTTCACAATCACATAGCTATATGCGCTAATAAGATTGGGGCGGCTCTCATTTCGAGAGCCGGATCTTTTGTTTGACAGACGTTGTAGTTGGTAGTAGCTACGATGTAGGCAACCACAGGGGCTCCGAAGTGAGTAGGGACTACTTAAAACATTTCCGGGGAGTAGAAGTAACGATTTGGAATACCGGGGAGGGTGGGCATCCGTCCCTTGGTGAAAATCTCGCAAGGGTTACACGACATTGAAATTCACCAAAAAATTCGCGGCGTATAGTAATCTTGCCCTACTTTGCTATGCCCTACTAGTGGGGGACTCAAATCGACTGGGTGCAGTCGGGAATAAGTTCAGCCCCGAGCCCGGGCCCACTCGTAGAGGCGCGCTCCCTTGGGTCTAGAGACATGAAGCGCACTCACGGTCCACAGTTTTGGTGGAAACGCTGTCAATACTACGTTCACTTGCCCATACGGTTATATAGGCGTTCGGAGCGCGATTGCAGGTGGGGATATAACCACAACGCGGGGGTGAGGACTGAACATCCATACCGGCTTGTACCGCGTGGGATCAAGACACTTGCTATCTGATCTAGCTTTTCGAAGTATTGCTCGGTGAAAGTAAATTGTGCCAGGTAGACTTATCACGTCCTGATACGGGAACATGGACTTGTTACTGCGGCGTTGAAATCAATCCTTGCTCTTCTGCCATGTGTATTGTGTTTCCACTCTGTATTTTGCCAGCGTCGTACATGGCACAGCTTTGACCCGTTGCAATGAGTAGGATATACAGTGTACTCGCGATTAAATGGTGTCACATGCCTTGGCGCCATGCGGTATAGTTCAGGCGCTCGTATTCCGGACCCGCTTCAAAATGGCGGCAATTGGTTGATGCTAACAAGATCCTCTAAGTGTGGGTTGTGTACTGTAACGTCCGTGAACTCTTAACTAGAAGAGAAATGCGACGCGACATGTTACCTGGACCTGTTCAGAAACGTGACTATCGCGGGCCGGGTATCCTATTAAAGGAAGCACCACCTTTAGCCTACGCTATAGCCAGATCAGCCACAAGACGTTTGACAAAGCGAATACCATGACATAAAGTCCGACAGGGCGGCAATGAGCTCAATATATTATTATTATGGACAGATTTTTAAGGTACGGCTTCAAGTCGATCGCACAACAGTGCTGCTTCGGTTCCCTGGAGCAGGCGTACAAGTACAAGCCTTTTAAGGAGACCAAGTTTATGAATATCTTGAGGCCCCCGCTCAGCAGTACCATTTATACTAAACGGAAGTCGCCTGGACAGTTAATTCGTACCTATATAGCCTGCTGGTAAGCGGCAAACCGCTGTGTCTGTACCATATTCAGGGAGTTCACGTGTCGATACCTCGAGTGCGGGAGCGCACCCGGGCGCCCTAATCGTGGTCGCCCTAGGTCTCCTCTACTATTGGGAAGCCATAACGCATCCTCCAGGGTTTGCCGGTAGCTTCCTTTGGTCGGTTAGCACTTCACTAATTTCGCGGTACTATTCAGTTGACGTCTGGCTCTCAAACGTTCTGGACTGTTACTCTTGCGGAGTTTATGCCCCCGATCTTAATTCGAAAATAGGCTTCAAAACGCTCCTTGTCAGGCCAGATATTCGTGCTGTCGGTGGCGCTGCAAGCGCAATACTGAACGCTCGCAGGTCCGATGGGCCCCCTGGTCATCTGTTCATCTTAGCAATAGAGGTCTTAATAGCAAGGACGTTACTATGTATCCTGCCCAGCTGAAGAAGTTCATCTCTGCTCACGTGTGACGCTAACTCGATCCATGTTAGCTCACTAGCGTTAGGCTGGGGGTCTCTGACCACCATGACTTCTCATGCGGGAGGGGCCGGCCCGATATTCATTGGGTCGTCGAAGCCCCTGCTTCTGTGACGACCTCGGTGGCCCAAAACGCACTGGGATGCAATCGGCTTTCCGTGGTCTCCCAGTAAAAACCGCCAGTTATTCTAGTTGCTGCTCGTTCCCTAGGTGGTTAAAGGTCCCCAACAACGAAATGGGTGAGTCTTACATTACGGACCTGGAAATCTACAAAAGCCTACGTACAAGTACGCACTTGGTCGTCAATGCCCTAGGCGGATGGGGACCCCCCGCCCTAGGTCCCAGATCCAGCTTCTTTGACGTCACATGTGCCATTATGGACTCTGTTCCGATAGGTCAATAGGAAGAATCACTCACTCTGATAAAAATTGACACCGCTGGGTCCTAGCTCGGCAAATGCAATCCCCCGGATAAGTCAACTCACGGGGCTTGCAACATCGCTTTGGAGAACGACTGATAGGCAACCGATCTGACGGACACGGCGCTGGAAAACTCAGGCATTACTCACCGTGCGAAATTCCTTTCCTAATTTCTTCTAACGGGACCTCCACGATTGCTGTTTTTGTATATGTGTTGCGAATGTTAAAACAGACAGGGATCAGACCTCTAGAATAATGGAACATTTAGGCCAAAGATCGCAGCTGCATTTTTTCCTCTTAGCTCTCGCACCTCGTTTAAGCATCGCGGAGCCTGAACGTATTCACTTGTATCCTCACCATTCTGCGTCATTATCGAGTGACCCCTGTCGAGCCAACGCGAGCTCTCAGTCTCGCGAATTCCGACGTTAACCAATAGTCTTAAGATTTTAATATATTCCGGAAGGTGTGTTACCCATTGATCACCAACGGTATGGGTGAAAGCTGTTGTAAAAGTCAGACTAGTTTAGAGCGAACCACATGGGGTCACATCTCATTAGAGAAAAGGAC + /04//11,-/-)(&&+***)/1,'0(%&$#$&&&1*+0,))'(%(%&%'4433324/.,''(++&12022..0./0&(331110233//1%$'((224.(1++)30/.+,0.1**/10-,-2.,%)****1.-.)0001-,-)+..,-,4,)($*'+0.23+&+)*310.+(%''(,3442////03(,')0,+13./-,*('($$&'',,/+*))(%'$+--.1-/032.2-,,+*%&&'&&(*,'&#('**')((-,)+()'(($'%$##%&),*)&&$"'$%,'$#'$(0,"#$)''*0/452550+,1--02)).+',,,245.-0..(,,.0,*.,+,)&%'(&&-*,&+2)%&,+,1-4.-0/.../*)2420&(&-.++$+)',,(-1+(01/2-+-)(#)+43/+-))('''('$')),-++*-4'%*),)*0--)++-,++(*'+')4200+-++&+$&')#'-03+/01443,*'),10.,+*-+%$%%$##$$&&+$+.-+''*$(+'(-0,/.+*',42131(',('(&)((,,-....1.*,)-3/1,,&##%%().34214-'++''+/0.+*+$+-240*0++..31-,/+-0--)--.,.(&+%%#('*))%)#'('&(*+***),,,....20/.-2,0.)'#%&(*./('$%$%&&'*+())*(#,(''+)(**)'($&(*'$)+,,+.++41*-2,2432213-31-.1.+&%&%&-+./.,,'%&&(-/10***(+1,+-,2)++*.+*,.,)*,,12/-+*&''*)+-+143/(('-,,,),012322,3,+,-0,-11,+3.,13,'#$*#-,++%042+,+&')..$),&#%#%$,)01--3*-(%&&$(%*0&*120.+0/301./'%%%&()+*,01&,01220121,-$&-0332**'++2+230.*$0*&')+)+&/.,'-&,1/+*(**.)+%)%)%.4144235/2222231454542/&24131,403144441*/'00/412111210+)'(+.31/3.0524041.4-1)/43/**)($,))),)-1-/04505-32533155443+,+++11+3+%'#$-./3,+,&%&*,-.--3.,0.%,--000.---,00522/-*)*'+$$$)$$,1123122*),)(2*.22*&$%')(.*$%&')+*'+%**1+)&)$&&*(&-3304*'$'%$)$%%&('')/**0+41012.,.4,)+-2/./.0445.+)+)*%(**'/,*.+((3-+*-('(.+('%&%&*)(*-(%+.112534.-./).3..34-/3201/,,5/,(*'((+)*2232(*'/.*.12)1,+)*+%""#%'&)*-2.)2)%&()).-(*%''%'#))*,*(()210)(''-.$#*+)-.4554231-*)&'*(431351130)$&',1)&%+)+)0%+033++.--...-,+/142-0+--0./0,)(/(),)++)+*&%$%%,)(,000-0/*)%-,013/(5,*0(0.)+)(.)3231*,+%'(%%%#)(),.110'-&*-+)'/&('(+.031101+.-.,,(.--53&2//.-)*+*+1-/+,-3,1.&('(.*+3()'#)#'*0//-('*))-)%('00/'+...321/,00-**'(./1-/01210111111+-1/1*-3/-'''$'#)'+,))(1+...042443120/04.--%(,-+)/.03(2+211.%(++-30+-*$'%$*+++--,'*++00-*('('#$'*.(,.%*/$00/.*(*),)+,,/,(&())''()+,*+%-/+,.-55/-.(*33.-+++22/1.*,*((*,./-(*)%.013//4/31*'.+)&('%$(-0.-,)+)++&&+03,0+2323445..3,)0,,24.*(/2-.-&,402534212*11/&'%%'/-+*)&13/4221455432.43,110355443333./#$'%)(('&),2,()(*)..450144.....3/.-*&./0./-&.()'$%)**--.,1//%+(%'))++,13./3.3201-()(,((&,,)%%,-.)3/25/*)1/)%%)++4334*+0-*%#&'''$$)+*+%+(++1%(%**4/21-12/'22,+1.-3+*2&1+,,.22030-*,+/0.32.2./''+%()*1*'&)%)*.2403321.()$&#$$*,-+',4**35321&('#%+*,,114345333322.*+54,((&)+401+&#$*,--4,+/,)'*&&-/42234-(-&,1.'.0./4224+&%(#$#$%&)(')0&+4323502)%%&(..3322-)%-0).-)('%*((.0/,/)/+'&*&&%',0++++,32324220.1'+,,/0(40-)(02.-,-./3//3.-/1**#%$'(//-+.+.3).1.((+)).10..&2.//12*)0$$,**++(&*,((2/345/50.3/-)&%'&%%()1,)(1-)/,(,'')*1((%11-221*4440533553232-5*//-,+)-,,$0,-+(0&+.3243///.*(%$*+04.)&%($'*2-+'+))+,'(&+-,-'&.%'+#''+)&(('%()%&*%',(--2)'%$&,03//+((&$%$)(/0442-)3/1211&))(%$+,./5-'+$--,+$$(302231/0.,+&&..-/.2.03/01,(,)%+&(0,+)*+.,&,',)$&,.12,,)*,+*%()1.20,.,0/*/32153,)3,,3)12/13/'',//0/,-.3244/++,'0*(.'()&..1233320/0134)$'&)2,,21/124('*()1+*(33-)453432343,*&%((.233./0*./-*((*..,,$*+))&4541111031&&&%&,*242/**%(+1-11/.2$15--2544540+)'&(#&'*1-.2/.10...)$&#**&('(),/+ @test_split_4 TCATTACGTTTGTATTTTTCCGGGTTGACAAGAACGCCCATCTCACCCCGGCTTGTATTGCGCCGTCAGGAAGGTTGCGAAAACCTCAATGCAGGCCTCAGCGCGGCGAAAGGCGCGACCGTGTTCACAATCACATAGCTATATGCGCTAATAAGATTGGGGCGGCTCTCATTTCGAGAGCCGGATCTTTTGTTTGACAGACGTTGTAGTTGGTAGTAGCTACGATGTAGGCAACCACAGGGGCTCCGAAGTGAGTAGGGACTACTTAAAACATTTCCGGGGAGTAGAAGTAACGATTTGGAATACCGGGGAGGGTGGGCATCCGTCCCTTGGTGAAAATCTCGCAAGGGTTACACGACATTGAAATTCACCAAAAAATTCGCGGCGTATAGTAATCTTGCCCTACTTTGCTATGCCCTACTAGTGGGGGACTCAAATCGACTGGGTGCAGTCGGGAATAAGTTCAGCCCCGAGCCCGGGCCCACTCGTAGAGGCGCGCTCCCTTGGGTCTAGAGACATGAAGCGCACTCACGGTCCACAGTTTTGGTGGAAACGCTGTCAATACTACGTTCACTTGCCCATACGGTTATATAGGCGTTCGGAGCGCGATTGCAGGTGGGGATATAACCACAACGCGGGGGTGAGGACTGAACATCCATACCGGCTTGTACCGCGTGGGATCAAGACACTTGCTATCTGATCTAGCTTTTCGAAGTATTGCTCGGTGAAAGTAAATTGTGCCAGGTAGACTTATCACGTCCTGATACGGGAACATGGACTTGTTACTGCGGCGTTGAAATCAATCCTTGCTCTTCTGCCATGTGTATTGTGTTTCCACTCTGTATTTTGCCAGCGTCGTACATGGCACAGCTTTGACCCGTTGCAATGAGTAGGATATACAGTGTACTCGCGATTAAATGGTGTCACATGCCTTGGCGCCATGCGGTATAGTTCAGGCGCTCGTATTCCGGACCCGCTTCAAAATGGCGGCAATTGGTTGGACCACTTAACGAAGGCTTTTCCACAATGCCCCTCGAAAGGATCCGCAACAACATAACACCCCAGTACAAGAGAGGGATAGTATCATTGCTGCTATATCTAAGACTCTCCATCGCATAGGAGCGGAGGTGTACCCCGAGGACACCTAGGTCAATCAACATCAATTCGTATGATAATCCACAATGAAGGGTTCTCCCTAACCCATGACATAAAGTCCGACAGGGCGGCAATGAGCTCAATATATTATTATTATGGACAGATTTTTAAGGTACGGCTTCAAGTCGATCGCACAACAGTGCTGCTTCGGTTCCCTGGAGCAGGCGTACAAGTACAAGCCTTTTAAGGAGACCAAGTTTATGAATATCTTGAGGCCCCCGCTCAGCAGTACCATTTATACTAAACGGAAGTCGCCTGGACAGTTAATTCGTACCTATATAGCCTGCTGGTAAGCGGCAAACCGCTGTGTCTGTACCATATTCAGGGAGTTCACGTGTCGATACCTCGAGTGCGGGAGCGCACCCGGGCGCCCTAATCGTGGTCGCCCTAGGTCTCCTCTACTATTGGGAAGCCATAACGCATCCTCCAGGGTTTGCCGGTAGCTTCCTTTGGTCGGTTAGCACTTCACTAATTTCGCGGTACTATTCAGTTGACGTCTGGCTCTCAAACGTTCTGGACTGTTACTCTTGCGGAGTTTATGCCCCCGATCTTAATTCGAAAATAGGCTTCAAAACGCTCCTTGTCAGGCCAGATATTCGTGCTGTCGGTGGCGCTGCAAGCGCAATACTGAACGCTCGCAGGTCCGATGGGCCCCCTGGTCATCTGTTCATCTTAGCAATAGAGGTCTTAATAGCAAGGACGTTACTATGTATCCTGCCCAGCTGAAGAAGTTCATCTCTGCTCACGTGTGACGCTAACTCGATCCATGTTAGCTCACTAGCGTTAGGCTGGGGGTCTCTGACCACCATGACTTCTCATGCGGGAGGGGCCGGCCCGATATTCATTGGGTCGTCGAAGCCCCTGCTTCTGTGACGACCTCGGTGGCCCAAAACGCACTGGGATGCAATCGGCTTTCCGTGGTCTCCCAGTAAAAACCGCCAGTTATTCTAGTTGCTGCTCGTTCCCTAGGTGGTTAAAGGTCCCCAACAACGAAATGGGTGAGTCTTACATTACGGACCTGGAAATCTACAAAAGCCTACGTACAAGTACGCACTTGGTCGTCAATGCCCTAGGCGGATGGGGACCCCCCGCCCTAGGTCCCAGATCCAGCTTCTTTGACGTCACATGTGCCATTATGGACTCTGTTCCGATAGGTCAATAGGAAGAATCACTCACTCTGATAAAAATTGACACCGCTGGGTCCTAGCTCGGCAAATGCAATCCCCCGGATAAGTCAACTCACGGGGCTTGCAACATCGCTTTGGAGAACGACTGATAGGCAACCGATCTGACGGACACGGCGCTGGAAAACTCAGGCATTACTCACCGTGCGAAATTCCTTTCCTAATTTCTTCTAACGGGACCTCCACGATTGCTGTTTTTGTATATGTGTTGCGAATGTTAAAACAGACAGGGATCAGACCTCTAGAATAATGGAACATTTAGGCCAAAGATCGCAGCTGCATTTTTTCCTCTTAGCTCTCGCACCTCGTTTAAGCATCGCGGAGCCTGAACGTATTCACTTGTATCCTCACCATTCTGCGTCATTATCGAGTGACCCCTGTCGAGCCAACGCGAGCTCTCAGTCTCGCGAATTCCGACGTTAACCAATAGTCTTAAGATTTTAATATATTCCGGAAGGTGTGTTACCCATTGATCACCAACGGTATGGGTGAAAGCTGTTGTAAAAGTCAGACTAGTTTAGAGCGAACCACATGGGGTCACATCTCATTAGAGAAAAGGAC + 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Filtlong-0.2.1/test/test_split.py000066400000000000000000000255321406722713100170250ustar00rootroot00000000000000""" Copyright 2017 Ryan Wick (rrwick@gmail.com) https://github.com/rrwick/Filtlong This module contains some tests for Filtlong. To run them, execute `python3 -m unittest` from the root Filtlong directory. This file is part of Filtlong. Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with Filtlong. If not, see . """ import unittest import os import subprocess def load_fastq(filename): reads = [] with open(filename, 'rb') as fastq: for line in fastq: stripped_line = line.strip() if len(stripped_line) == 0: continue if not stripped_line.startswith(b'@'): continue name = stripped_line[1:].split()[0] sequence = next(fastq).strip() _ = next(fastq) qualities = next(fastq).strip() reads.append((name, sequence, qualities)) return reads class TestSplit(unittest.TestCase): def run_command(self, command): binary_path = os.path.join(os.path.dirname(os.path.dirname(__file__)), 'bin', 'filtlong') input_path = os.path.join(os.path.dirname(__file__), 'test_split.fastq') assembly_reference = os.path.join(os.path.dirname(__file__), 'test_reference.fasta') illumina_reference_1 = os.path.join(os.path.dirname(__file__), 'test_reference_1.fastq.gz') illumina_reference_2 = os.path.join(os.path.dirname(__file__), 'test_reference_2.fastq.gz') command = command.replace('filtlong', binary_path) command = command.replace('INPUT', input_path) command = command.replace('ASSEMBLY', assembly_reference) command = command.replace('ILLUMINA_1', illumina_reference_1) command = command.replace('ILLUMINA_2', illumina_reference_2) output_name = 'TEMP_' + str(os.getpid()) command = command.replace('OUTPUT', output_name) try: self.output_file = [x for x in command.split() if output_name in x][0] except IndexError: self.output_file = '' p = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) _, err = p.communicate() return err.decode() def tearDown(self): if os.path.isfile(self.output_file): os.remove(self.output_file) def check_one_read(self, read, length, start_seq, end_seq): self.assertEqual(len(read[1]), length) self.assertEqual(len(read[2]), length) self.assertTrue(read[1].startswith(start_seq)) self.assertTrue(read[1].endswith(end_seq)) def test_split_1(self): """ When splitting is off, the reads aren't split. """ console_out = self.run_command('filtlong --min_length 1 -a ASSEMBLY INPUT > OUTPUT.fastq') self.assertTrue('4 reads (11,600 bp)' in console_out) self.assertTrue('after splitting' not in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 4) def test_split_2(self): """ When the split threshold is over 200, no reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 250 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 4 reads (11,600 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 4) for i in range(0, 4): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') def test_split_3(self): """ When the split threshold is over 200, no reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 201 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 4 reads (11,600 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 4) for i in range(0, 4): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') def test_split_4(self): """ When the split threshold is exactly 200, only the last read is split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 200 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 5 reads (11,400 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 5) for i in range(0, 3): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[3], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[4], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_5(self): """ When the split threshold is between 150 and 200, only the last read is split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 175 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 5 reads (11,400 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 5) for i in range(0, 3): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[3], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[4], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_6(self): """ When the split threshold is between 50 and 100, tbe last two reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 75 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 6 reads (11,300 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 6) for i in range(0, 2): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[2], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[3], 1800, b'GAAACGTG', b'AAAAGGAC') self.check_one_read(split_reads[4], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[5], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_7(self): """ When the split threshold is between 50 and 100, tbe last two reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 51 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 6 reads (11,300 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 6) for i in range(0, 2): self.check_one_read(split_reads[i], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[2], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[3], 1800, b'GAAACGTG', b'AAAAGGAC') self.check_one_read(split_reads[4], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[5], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_8(self): """ When the split threshold is exactly 50, tbe last three reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 50 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 7 reads (11,250 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 7) self.check_one_read(split_reads[0], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[1], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[2], 1850, b'TGATGAAT', b'AAAAGGAC') self.check_one_read(split_reads[3], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[4], 1800, b'GAAACGTG', b'AAAAGGAC') self.check_one_read(split_reads[5], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[6], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_9(self): """ When the split threshold is less than 50, tbe last three reads are split. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 25 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 7 reads (11,250 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 7) self.check_one_read(split_reads[0], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[1], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[2], 1850, b'TGATGAAT', b'AAAAGGAC') self.check_one_read(split_reads[3], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[4], 1800, b'GAAACGTG', b'AAAAGGAC') self.check_one_read(split_reads[5], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[6], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_10(self): """ Same as the previous test, but using reads as a reference. """ console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --split 25 INPUT > OUTPUT.fastq') self.assertTrue('after splitting: 7 reads (11,250 bp)' in console_out) split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 7) self.check_one_read(split_reads[0], 2900, b'TCATTACG', b'AAAAGGAC') self.check_one_read(split_reads[1], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[2], 1850, b'TGATGAAT', b'AAAAGGAC') self.check_one_read(split_reads[3], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[4], 1800, b'GAAACGTG', b'AAAAGGAC') self.check_one_read(split_reads[5], 1000, b'TCATTACG', b'ATTGGTTG') self.check_one_read(split_reads[6], 1700, b'CCATGACA', b'AAAAGGAC') def test_split_names(self): """ Makes sure the split reads are correctly named. """ console_out = self.run_command('filtlong -a ASSEMBLY --split 25 INPUT > OUTPUT.fastq') split_reads = load_fastq(self.output_file) self.assertEqual(len(split_reads), 7) self.assertEqual(split_reads[0][0], b'test_split_1') self.assertEqual(split_reads[1][0], b'test_split_2_1-1000') self.assertEqual(split_reads[2][0], b'test_split_2_1051-2900') self.assertEqual(split_reads[3][0], b'test_split_3_1-1000') self.assertEqual(split_reads[4][0], b'test_split_3_1101-2900') self.assertEqual(split_reads[5][0], b'test_split_4_1-1000') self.assertEqual(split_reads[6][0], b'test_split_4_1201-2900') Filtlong-0.2.1/test/test_split.txt000066400000000000000000000004001406722713100171770ustar00rootroot00000000000000test_split_1: all good (2900 good bases) test_split_2: 1000 good bases, then 50 bad bases, then 1850 good bases test_split_3: 1000 good bases, then 100 bad bases, then 1800 good bases test_split_4: 1000 good bases, then 200 bad bases, then 1700 good bases Filtlong-0.2.1/test/test_trim.fastq000066400000000000000000000232161406722713100173300ustar00rootroot00000000000000@test_trim_1 GCCCTGGCTAGCACTGCACTTCGCACGCAAGTTTCATCCAGTAACGGCGCCGATAGTCCTATTTCGCAGCGCTCTCTATTGTCCGGCGTCCGGGGTAAGGATTTGCAGACATCCCGCCTACTATGATACTATGACTCCTTGTATGTCCTGTCTCAGTTAATGGTTAGACATGTTGAGATGACTGGTTGAACTCAGTAGGTTATGCTAAGACTCCATCAGTTTATTCAACAATTAGAATTGGATCGAAGGGGGATACCCGGCAATGAAGAAGAGCCCGTTAGCCAAAAATAAGGTAGCAACTACAAAGTAGACGCGCAGAGCTAAATAGGTCGTACTTGATATTGCAACATGACCAACCGATGCAAAGTCCGCTCCTGCATATTCGTATAGGATGGGGGCCTCGGGGCCACGAAATGGCTCCATGCTTCGCAATCACCGCACCTTTAAGTAATCGCCGCGTATTTCCAAAAAGCCGGCATGGGTGCAGATTAATTGGGACTTTGTCGAAGACGTTACACAATCCCGCCAACTCGAATGGTTAATGTTCAGCGGATACACGCTCGCTGGCGTCCACGGATCTATCGCTTTGCTCCCGCTCGGCTCCCGAACGACGGGACTCGAGTAACCGATCACTCCGCTTTTCTCAACCTCAGCTTTTGGTGCCACAGCAGAGTGACACTCTCATGTTGGAGTATGGGGGTCCCTGCTAATGACCGCGGACGACTAGCAGTTATTTTCTGCGCGTCACTCAGAATAGCGATTGTATAAGCAAGGCAACCGGCGAGGTCGATAGCGTGTAGCTGCTCGGACGAATCTATATAGGTAGGGGCCTGCCTGTTCTTTCCCCACCCTGTATAGCAACAGAATAGGCTGTCCTAATTTTGGGCCCCACACTTGGAATACCTGTAAAATCTTTAGCTTGGCTATCGATATATCATGTAACGAGCTGTGAAGTACTTTATTCAAGAGAGCACCGTGGGACCGAGATAAGATCGTCAAGGACGTTTACCAATTGAGCGTTGGTCTTATCACTCCGATCGCAGAACTATATCTGGCGATAGTGTATCCGGTAGAGTGCTCCCATTCTAGTCTGTAAGAAATTGGAGCACGACTTTTCCAGTCATTTATCGAAACCGGCCGGTGGAGTAGTAGTAATTACTTTACTCAATACGCTTTGACCTGATTCATGCCCCCAAAAGTGGGGTACGTGAGTCTAAGTACCTGTGGAGTGCTTTTCCGCCGTACATAGTTAAGTATAGACTTATTCTACAGCTACCCGCGCGGGGGAGAAAGGGTCCAG + /04//11,-/-)(&&+***)/1,'0(%&$#$&&&1*+0,))'(%(%&%'4433324/.,''(++&12022..0./0&(331110233//1%$'((224.(1++)30/.+,0.1**/10-,-2.,%)****1.-.)0001-,-)+..,-,4,)($*'+0.23+&+)*310.+(%''(,3442////03(,')0,+13./-,*('($$&'',,/+*))(%'$+--.1-/032.2-,,+*%&&'&&(*,'&#('**')((-,)+()'(($'%$##%&),*)&&$"'$%,'$#'$(0,"#$)''*0/452550+,1--02)).+',,,245.-0..(,,.0,*.,+,)&%'(&&-*,&+2)%&,+,1-4.-0/.../*)2420&(&-.++$+)',,(-1+(01/2-+-)(#)+43/+-))('''('$')),-++*-4'%*),)*0--)++-,++(*'+')4200+-++&+$&')#'-03+/01443,*'),10.,+*-+%$%%$##$$&&+$+.-+''*$(+'(-0,/.+*',42131(',('(&)((,,-....1.*,)-3/1,,&##%%().34214-'++''+/0.+*+$+-240*0++..31-,/+-0--)--.,.(&+%%#('*))%)#'('&(*+***),,,....20/.-2,0.)'#%&(*./('$%$%&&'*+())*(#,(''+)(**)'($&(*'$)+,,+.++41*-2,2432213-31-.1.+&%&%&-+./.,,'%&&(-/10***(+1,+-,2)++*.+*,.,)*,,12/-+*&''*)+-+143/(('-,,,),012322,3,+,-0,-11,+3.,13,'#$*#-,++%042+,+&')..$),&#%#%$,)01--3*-(%&&$(%*0&*120.+0/301./'%%%&()+*,01&,01220121,-$&-0332**'++2+230.*$0*&')+)+&/.,'-&,1/+*(**.)+%)%)%.4144235/2222231454542/&24131,403144441*/'00/412111210+)'(+.31/3.0524041.4-1)/43/**)($,))),)-1-/04505-32533155443+,+++11+3+%'#$-./3,+,&%&*,-.--3.,0.%,--000.---,00522/-*)*'+$$$)$$,1123122*),)(2*.22*&$%')(.*$%&')+*'+%**1+)&)$&&*(&-3304*'$'%$)$%%&('')/**0+41012.,.4,)+-2/./.0445.+)+)*%(**'/,*.+((3-+*-('(.+('%&%&*)(*-(%+.112534.-./).3..34 @test_trim_2 ACCTGTTCAATTACCGAGCAGATTTATACAGCAGGAGTCCGATGCGGAAAAACGGGCCAGGCTCCATTTAATGCGAGCAAAAACCTGTCCCACCTTGAGTCGAGGATTACGAAAGAGTTGCAGCGCAAGCCGTTCACGCTGATATAAAGTCCAGCGCTGAAGACGGTGGGACGCCCAGGTAGTACAATTCTTGCTGAGAGCTTCGTCCTTGCAACGCATCCGAGTCCAATGATCACATAGTTGCAGTGGGCAAGCGGGCGGTGAGCAAAACAAACAATGCTATGGGTCTTAACGAGAACAGTCCGCCGTGGTTTTAGTGGCTCTCCTGCAGGTCACCGGTCAGCGTTAAGTACTGGCGTCAGCGTGAATGTACAGATTCCCTATGGTTGGCGAGTGATGCCCACCGTAGTACTATCGGTTAATGTTAGGAAGAGCGTAGAAGTGCCTACCTTGGATCGGGTATGGAGGGAAAGAGATCAGCAATATCAGGACCACGTGGTACTAGGGTTGGCGCGCGTATAACCCTAATCATTAAAACATCGCAAGGATAGCAAGATGATCTGAACGGGTGTATCACGTATCCCGAGTAGGTCAGTTTTAAGGACCACTGCATTGGACCTTTCGCGATAGGCTCAGGATCGCTAACTGTTATTAGGTGCGTAAAATGCAAGATCTGGACGTCCTAATGCACGCATGGCGAC + 1-..()(+%%''*''&$$'00(&'&&$)*.01220.*###)(*)+*/0/..+0/-(&()&))%**(*'&++-(-.)&$%'%()+-/124341--4--/+*22')%*%'&&$&')(&&%$%%%'),-0*++&%(1*,1&&%+'''%$,**%'),,(++('*11,0/31,+*,%)+-*'%-.1.1$+/-/343,))'&&*),0/2,&(/0,)&,+0(&&+'.14///--..23'&$##).+*/+*,1--&,+,.,--4014*'))%&)&$"##'%$')+,,&#')..(-+.)(+,*0$-.'&%&(*-,%&'*&*4103512333.-,)&'&&*+,1'*)(33-*,*)(/10,1000/*'%*(01.+-+/3132+('($$+*)/213225,&,'&*435541,*32))'&../0-01.23421.1-2/+*(-0533433-/.-3220.1.*./+/0.-)-0,)***')*#%#&*$--.1*+/+0321,+01-+//0+****,143+0.))'%#')/.,,+,(,,3+*)'&%(%+.0*../0--/,..0))&%'')&'(&+.123-3/+*(&'&%$&)*((-,.,,'+3/,,+.,)21)'&''$+,,(&(+-214+.*(&#*,/031+%%%&())-&'(&&)'&,(+3.+3121./0-03%/42+1/)*/+043315322+1,*)'33,+-01'-,/$-/2-1/- @test_trim_3 CTTGAACAGGCGAGAACGCCAAAGGCATACTGATGGCTAAATGACCTTGACCATATGCCGCGTTGTCGAATGCGAGTGGAAAACACCCAGGCCTAGCTACGGAGACCTTAAAAACCAAAATATTATAGGACGCATGGTTGCTCCCCGCCGAGTTGCGCTTCACCGCGTCACTTTGTTGATATATTTGAATGAATTCTCTGACTGCCAGAAAGACTCATGCACTGCTCTTTAGTAGTGCAGGCCGAGAAAATTCCGAGCGAACCCTGTCCGCGCCTTCCTGACACATCTTTCCTTCCGGAGTGGGCTTAGTATGGCTTCCGAATAGCCGAGCTACGTTGTTTTGATAGGAAAACCGCCAGTTGTTTATAAAAATGGGGTGCCCTATGGACGTCCGATTTGGTATCACTGACTATCTCCGCTTCACTGTACGGGGGATACACCAGAGTAACTGGGCAATAAGTGCCAAAGATTAGTTGCGAACGACTCACGTTCATGGTCAATTCGTTATCCTTAAAGTTATTGCCTGCTACCATAGCTTATCAGAACGAGCGGCCCTTTGAGTCGCCCGTGAGAAGACCGATTCCAGACTAGCAATATAGATTTGTGGGGTTAAAAGGCTGCCTCCGTGTAGTCTTTCAAAAAAGCACGTAAATCTGTAGTGGGCCTTTGGATATTGTAGGGGGAAAAGCGGAGACATTCAAGAGGCAGTTTTTTGCGTTTGTATGGCAGGCCATTTTAGAGCCTTTAAGTGGCACATAACCGCGGAAGCTTGGAATGGAAACGGGATCTAAGTCGCATTCAATTTCTTGATGAGTCTGGCGTTCGGTGGCGTGGCTCTTAGGTGAGAACGACTGACATACCCTGCTGCTGAATCCGTGAGAAACCGAACTTGGCGCAATCTTTAGCAGGCGTACACATGGGCGCGAGACGTGCGGTAGCGAGCAAGAGCTGCTAAGTGAGTCTCCTCCAGCGCTCCCACAGGCCAGGTCATGACACGTTG + -/153.461)+-**+,31(-'$&)%3+*3,%.%11012+./*+.-142411001++1+&&.14-'-%,,0+,**(),,,0,)*00+++*+,,+()/14323****(%%/$)*%-.5.++.))*3+.,&'$'&(+-./+*-+-((+(3./..+(&%&(,)/1/.0*+)(+&)-(+-%)&''+-0++'$(+01+3410)/(#&#)&01.'&#%.+13131-%&%$'+//&.*/++,0)&%%$#($,-31/32--+**(&$#"'$&)'$/10114224-1)&*'21**311,''*%*112//*&-)./334443-,3,+,,52)4104234430/&/1234'.1-$-***,&**-*++.,0++,((+0++4531,%+,0**1/**3135344*--$%#$('-+0*(+,0.,,0.-3/,.)+2200501221)&%00*,+/./52,2-./(&%&%#))()'&#'#,+(-24/234231,.&)*-$),%&,+-0+(()-(&2&,/-+.-234232200122.,,+**+1+10-,*0/2)2)+-2.//,-++3+'.-0-03312,)*+,-*#'&'%-%(%%$%()(-+.4/(+($(+011..//214234410//.(*+).,/2+,,545105335,((''#$%()-0)(+.*'%+&+224310),2130%$'(#$%*.12.100+%01,,4434-,4,2*3+(30).,++)'()(&-.0()*).-%210-/*(//101--0(&%)(,)&((()(&++-,&*.--...,'%&),240/42.($$%(&(44210,+-/1)'()(%&&&.,31/2&%#%%%033425.4204%.35120.(+..0-1$(,*(()*+*001342342*+0+*-(&&)--*+.01'/%()(),.-+..*.../02../12013)),1.)/1/22331.,%)((+,330.,$%*)2./51332,3,+)&/00132)'%(')'($%%%'%),0*24/03%//433311*0-.1,*,,,)(&$ @test_trim_4 AGGATGAAAGTGCCTTTCTTAACCATTTGTGCGCTGTCGAAGTTTACGAGGCCGCGGAGCCTGCTGCCGACATATGACCCCCGTGATCTTTGCTATATTCGGCTGCCGCCCGCTAACCGTATTAGTCGCCACTTTAGGCGACAAGTATTCCTGTACAGGCTGGTTCTTTCTGCTATCATCACACCGTGGGAATCGAGAGCCGGAATCGCTGCTGCTTCCAAAGGAGTGATGTTTATTTGACAACAGGGGTCCATAGGGTGTAGTAGAATTGTCCCGTTGGGCTTTAATTCGGGCTAGAGCCTTAGTCTAGGTTGAGTACATTTGATACACAACTTATGGCTCTAACGTCATCATCTTCCGCGCGCCTTCCGTTTCGGGCACTGGGTTAATTATTGAGGTCGAGGACAAAGACGAGTGGAGGTCAACCTTATCAGTATGATCTCAATTTATATATGCACAGCGCCAAACACCGACTCAAGGTACGCCACTGGCCACTCGGCACGCGGGCTCAAACTCAAACCAAGGTGACGTGGTACCCAGACTCCTAGATTGCAAGACTATCCCCAATGCTTTTGGCGGTCTCATCTACTCGAAAATTGTCTGTGCGGGGGCGTGGCATTTCTCCCACCTGCGGCCCTAAGGAGGAAGCATAGTACATCACTGGAAGCGACAAATGGACATGTGAATCTTTTGCCAGTATCTGAAGCGCTCCCCGCAGCCTTACCAACTTACAACTAAAGGCACCTCGAATCGCCTGGCGACGGCTCATTCGCGCTTCGATAAGGTCCTATAATCTAGGTGGTACCCGTACTCACAGTACGATATCGTTGGCCGGTAGTGAATAATCTACTATTCTAAACACGACTCTGGACTGTTTTGTAAATGGACACCGCCATACTTATTTGACTTCCTTTCACATTGTAGATGGGAGCCCGCTTGCGGACGGCTTTTATCGTTTGCACAACACAGGAAGGATCCATACTTATGGTGATTTTTTCTAACTACGCTGCATATGGAAAAGAGTCCAGGTGCTTGCAGAAGCTGCAAAAAAGGACGCCACGTCGCCCGTAACGAAGGGCAGGGCGGGTCTTTGATATACGCTCGCCATCTTGAAATAGGCTTCCCACGGACTGAACGGCGCCGCGCTCGGCAAGCATTTCGTGCCCGTCAAGGAAGCGCGTATCTATGGGTCAGTATTAGCATGTGACAGTTGACTTTGAGAGCCACAGCGTCTCACCCCTCGGGTCCCATAGTTATTTATCGACTATGGCCCGTGCAAGCAGTTTCCTTAGAGAATCGTTCAATTCATCCTTGAATATCTTCGTGACTATTATCACTAGTAAACAGTGCTCGCTCCGTTCTCCACCGCCTAGCACCGACGGTATTACCTCGGTACACCCCTACTCCGAAATGCACAATTACCATACTTCCATGCTCAGGTGCCATCGTATGAAGCCTACGCGTCTCGTAACGGGACAAGCCCTGAAATTACGGGGACAACACGAGCACATTTGTGTAGCTATGCCAGATGGGAATTAGGCTCATTCGGAAATTTGTTGCGAGAATCTTTCCGTCCTGATGATTGAAGGGAATGGCAACGGGGAGATTCTTTTCTCGCCGCAGGAAAGAATGACCGGTAACTTGAGGTCCCGCAAAAATTGAGCGGAGATCTGCAAGAATAGAATTAATTACATCCTGTCGACCTCGTTAGAAGGAGTAATACATACCGGAGGATCAAAGGGGCAGTAACTTAAAGCATGAGTTAGTGAACAACGTTGCCGGTCTTCAAAAGTTCAACTAGGTGCTGGGATCTGGTGTAGGAGCTGGCTAAAGGATCCTCGGCTAGGTGCCGGGCTCTCAAGCCCGGCGACAGTCTTTGATCACCACGGACTTGTTTACG + -/3201/,,5/,(*'((+)*2232(*'/.*.12)1,+)*+%""#%'&)*-2.)2)%&()).-(*%''%'#))*,*(()210)(''-.$#*+)-.4554231-*)&'*(431351130)$&',1)&%+)+)0%+033++.--...-,+/142-0+--0./0,)(/(),)++)+*&%$%%,)(,000-0/*)%-,013/(5,*0(0.)+)(.)3231*,+%'(%%%#)(),.110'-&*-+)'/&('(+.031101+.-.,,(.--53&2//.-)*+*+1-/+,-3,1.&('(.*+3()'#)#'*0//-('*))-)%('00/'+...321/,00-**'(./1-/01210111111+-1/1*-3/-'''$'#)'+,))(1+...042443120/04.--%(,-+)/.03(2+211.%(++-30+-*$'%$*+++--,'*++00-*('('#$'*.(,.%*/$00/.*(*),)+,,/,(&())''()+,*+%-/+,.-55/-.(*33.-+++22/1.*,*((*,./-(*)%.013//4/31*'.+)&('%$(-0.-,)+)++&&+03,0+2323445..3,)0,,24.*(/2-.-&,402534212*11/&'%%'/-+*)&13/4221455432.43,110355443333./#$'%)(('&),2,()(*)..450144.....3/.-*&./0./-&.()'$%)**--.,1//%+(%'))++,13./3.3201-()(,((&,,)%%,-.)3/25/*)1/)%%)++4334*+0-*%#&'''$$)+*+%+(++1%(%**4/21-12/'22,+1.-3+*2&1+,,.22030-*,+/0.32.2./''+%()*1*'&)%)*.2403321.()$&#$$*,-+',4**35321&('#%+*,,114345333322.*+54,((&)+401+&#$*,--4,+/,)'*&&-/42234-(-&,1.'.0./4224+&%(#$#$%&)(')0&+4323502)%%&(..3322-)%-0).-)('%*((.0/,/)/+'&*&&%',0++++,32324220.1'+,,/0(40-)(02.-,-./3//3.-/1**#%$'(//-+.+.3).1.((+)).10..&2.//12*)0$$,**++(&*,((2/345/50.3/-)&%'&%%()1,)(1-)/,(,'')*1((%11-221*4440533553232-5*//-,+)-,,$0,-+(0&+.3243///.*(%$*+04.)&%($'*2-+'+))+,'(&+-,-'&.%'+#''+)&(('%()%&*%',(--2)'%$&,03//+((&$%$)(/0442-)3/1211&))(%$+,./5-'+$--,+$$(302231/0.,+&&..-/.2.03/01,(,)%+&(0,+)*+.,&,',)$&,.12,,)*,+*%()1.20,.,0/*/32153,)3,,3)12/13/'',//0/,-.3244/++,'0*(.'()&..1233320/0134)$'&)2,,21/124('*()1+*(33-)453432343,*&%((.233./0*./-*((*..,,$*+))&4541111031&&&%&,*242/**%(+1-11/.2$15--2544540+)'&(#&'*1-.2/.10...)$&#**&('(),/++221.((&'1-4-/1/-))'*+/0,)-*.2545311*00)00)-+(*%&&%$&(,)-0/.)*.,-.--20/+)''$&*/.24331(,-,,03)+()1(&&$#'--,.-)*)+/5313.*--+('(&&.**/0--2/+1(++21(-112,%-.+)%$'%&#$$)-,.**3/,,-**')+*230011/+*)*)0.-**'')0-++23,3333522,/(+)*..)142+('/.-211'+$%'*1,2.*+1344+-'&)&'/4322..,(+((-'*.-')%&&'-34.-51))')(.4/*)&(, Filtlong-0.2.1/test/test_trim.py000066400000000000000000000116311406722713100166400ustar00rootroot00000000000000""" Copyright 2017 Ryan Wick (rrwick@gmail.com) https://github.com/rrwick/Filtlong This module contains some tests for Filtlong. To run them, execute `python3 -m unittest` from the root Filtlong directory. This file is part of Filtlong. Filtlong is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. Filtlong is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. You should have received a copy of the GNU General Public License along with Filtlong. If not, see . """ import unittest import os import subprocess def load_fastq(filename): reads = [] with open(filename, 'rb') as fastq: for line in fastq: stripped_line = line.strip() if len(stripped_line) == 0: continue if not stripped_line.startswith(b'@'): continue name = stripped_line[1:].split()[0] sequence = next(fastq).strip() _ = next(fastq) qualities = next(fastq).strip() reads.append((name, sequence, qualities)) return reads class TestTrim(unittest.TestCase): def run_command(self, command): binary_path = os.path.join(os.path.dirname(os.path.dirname(__file__)), 'bin', 'filtlong') input_path = os.path.join(os.path.dirname(__file__), 'test_trim.fastq') assembly_reference = os.path.join(os.path.dirname(__file__), 'test_reference.fasta') illumina_reference_1 = os.path.join(os.path.dirname(__file__), 'test_reference_1.fastq.gz') illumina_reference_2 = os.path.join(os.path.dirname(__file__), 'test_reference_2.fastq.gz') command = command.replace('filtlong', binary_path) command = command.replace('INPUT', input_path) command = command.replace('ASSEMBLY', assembly_reference) command = command.replace('ILLUMINA_1', illumina_reference_1) command = command.replace('ILLUMINA_2', illumina_reference_2) output_name = 'TEMP_' + str(os.getpid()) command = command.replace('OUTPUT', output_name) try: self.output_file = [x for x in command.split() if output_name in x][0] except IndexError: self.output_file = '' p = subprocess.Popen(command, stdout=subprocess.PIPE, stderr=subprocess.PIPE, shell=True) _, err = p.communicate() return err.decode() def tearDown(self): if os.path.isfile(self.output_file): os.remove(self.output_file) def check_one_read(self, read, length, start_seq, end_seq): self.assertEqual(len(read[1]), length) self.assertEqual(len(read[2]), length) self.assertTrue(read[1].startswith(start_seq)) self.assertTrue(read[1].endswith(end_seq)) def test_trim_1(self): """ Tests read trimming console output using an assembly reference. """ console_out = self.run_command('filtlong -a ASSEMBLY --trim INPUT > OUTPUT.fastq') self.assertTrue('4 reads (4,901 bp)' in console_out) self.assertTrue('after trimming: 4 reads (4,824 bp)' in console_out) def test_trim_2(self): """ Tests read trimming console output using Illumina read references. """ console_out = self.run_command('filtlong -1 ILLUMINA_1 -2 ILLUMINA_2 --trim INPUT > OUTPUT.fastq') self.assertTrue('4 reads (4,901 bp)' in console_out) self.assertTrue('after trimming: 4 reads (4,824 bp)' in console_out) def test_trim_3(self): """ Actually loads the trimmed reads to verify that they look good. """ console_out = self.run_command('filtlong -a ASSEMBLY --trim INPUT > OUTPUT.fastq') trimmed_reads = load_fastq(self.output_file) self.assertEqual(len(trimmed_reads), 4) self.check_one_read(trimmed_reads[0], 1300, b'GCCCTGGC', b'GGGTCCAG') self.check_one_read(trimmed_reads[1], 701 - 20, b'GATTTATA', b'ATGGCGAC') self.check_one_read(trimmed_reads[2], 1000 - 30, b'CTTGAACA', b'TCCTCCAG') self.check_one_read(trimmed_reads[3], 1900 - 27, b'CCTTTCTT', b'TGATCACC') def test_trim_names(self): """ Makes sure the trimmed reads are correctly named. """ console_out = self.run_command('filtlong -a ASSEMBLY --trim INPUT > OUTPUT.fastq') trimmed_reads = load_fastq(self.output_file) self.assertEqual(len(trimmed_reads), 4) self.assertEqual(trimmed_reads[0][0], b'test_trim_1') self.assertEqual(trimmed_reads[1][0], b'test_trim_2_21-701') self.assertEqual(trimmed_reads[2][0], b'test_trim_3_1-970') self.assertEqual(trimmed_reads[3][0], b'test_trim_4_13-1885') Filtlong-0.2.1/test/test_trim.txt000066400000000000000000000002701406722713100170240ustar00rootroot00000000000000test_trim_1: all good (no trimming) test_trim_2: 20 bogus bases at the start test_trim_3: 30 bogus bases at the end test_trim_4: 12 bogus bases at the start, 15 bogus bases at the end